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Protein

NADPH oxidase 1

Gene

Nox1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H+ ions as part of its electron transport mechanism.

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

The oxidase activity is potentiated by NOXA1 and NOXO1.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi101Iron (heme axial ligand)Curated1
Metal bindingi115Iron (heme axial ligand)Curated1
Metal bindingi208Iron (heme axial ligand)Curated1
Metal bindingi220Iron (heme axial ligand)Curated1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi337 – 343FADSequence analysis7

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • superoxide-generating NADPH oxidase activity Source: RGD

GO - Biological processi

  • positive regulation of reactive oxygen species metabolic process Source: RGD
  • positive regulation of smooth muscle cell proliferation Source: BHF-UCL
  • response to reactive oxygen species Source: RGD
  • signal transduction Source: RGD
  • superoxide anion generation Source: BHF-UCL

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Transport
LigandFAD, Flavoprotein, Heme, Iron, Metal-binding, NADP

Protein family/group databases

PeroxiBasei5408 RnoNOx01

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH oxidase 1 (EC:1.-.-.-)
Short name:
NOX-1
Alternative name(s):
Mitogenic oxidase 1
Short name:
MOX-1
NADH/NADPH mitogenic oxidase subunit P65-MOX
NOH-1
Gene namesi
Name:Nox1
Synonyms:Mox1, Noh1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620598 Nox1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 31HelicalSequence analysisAdd BLAST23
Topological domaini32 – 44ExtracellularSequence analysisAdd BLAST13
Transmembranei45 – 69HelicalSequence analysisAdd BLAST25
Topological domaini70 – 102CytoplasmicSequence analysisAdd BLAST33
Transmembranei103 – 123HelicalSequence analysisAdd BLAST21
Topological domaini124 – 167ExtracellularSequence analysisAdd BLAST44
Transmembranei168 – 188HelicalSequence analysisAdd BLAST21
Topological domaini189 – 206CytoplasmicSequence analysisAdd BLAST18
Transmembranei207 – 227HelicalSequence analysisAdd BLAST21
Topological domaini228 – 395ExtracellularSequence analysisAdd BLAST168
Transmembranei396 – 416HelicalSequence analysisAdd BLAST21
Topological domaini417 – 563CytoplasmicSequence analysisAdd BLAST147

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075231

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002101491 – 563NADPH oxidase 1Add BLAST563

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi161N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi241N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9WV87
PRIDEiQ9WV87

PTM databases

iPTMnetiQ9WV87
PhosphoSitePlusiQ9WV87

Expressioni

Tissue specificityi

Expressed in vascular smooth muscle cells.

Interactioni

Subunit structurei

NOX1, NOXA1, NOXO1, RAC1 and CYBA forms a functional multimeric complex supporting ROS production. Interacts with NOXA1 and NOXO1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000065995

Structurei

3D structure databases

ProteinModelPortaliQ9WV87
SMRiQ9WV87
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 282Ferric oxidoreductaseAdd BLAST229
Domaini283 – 390FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST108

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni396 – 535Interaction with NOXO1By similarityAdd BLAST140

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0039 Eukaryota
ENOG410XNZY LUCA
HOVERGENiHBG003760
InParanoidiQ9WV87
KOiK08008
PhylomeDBiQ9WV87

Family and domain databases

InterProiView protein in InterPro
IPR000778 Cyt_b245_heavy_chain
IPR013112 FAD-bd_8
IPR017927 Fd_Rdtase_FAD-bd
IPR013130 Fe3_Rdtase_TM_dom
IPR013121 Fe_red_NAD-bd_6
IPR029650 NOX1
IPR017938 Riboflavin_synthase-like_b-brl
PANTHERiPTHR11972:SF71 PTHR11972:SF71, 1 hit
PfamiView protein in Pfam
PF08022 FAD_binding_8, 1 hit
PF01794 Ferric_reduct, 1 hit
PF08030 NAD_binding_6, 1 hit
PRINTSiPR00466 GP91PHOX
SUPFAMiSSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit

Sequencei

Sequence statusi: Complete.

Q9WV87-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNWLVNHWL SVLFLVSWLG LNIFLFVYVF LNYEKSDKYY YTREILGTAL
60 70 80 90 100
ALARASALCL NFNSMVILIP VCRNLLSFLR GTCSFCNHTL RKPLDHNLTF
110 120 130 140 150
HKLVAYMICI FTAIHIIAHL FNFERYSRSQ QAMDGSLASV LSSLFHPEKE
160 170 180 190 200
DSWLNPIQSP NVTVMYAAFT SIAGLTGVVA TVALVLMVTS AMEFIRRNYF
210 220 230 240 250
ELFWYTHHLF IIYIICLGIH GLGGIVRGQT EESMSESHPR NCSYSFHEWD
260 270 280 290 300
KYERSCRSPH FVGQPPESWK WILAPIAFYI FERILRFYRS RQKVVITKVV
310 320 330 340 350
MHPCKVLELQ MRKRGFTMGI GQYIFVNCPS ISFLEWHPFT LTSAPEEEFF
360 370 380 390 400
SIHIRAAGDW TENLIRTFEQ QHSPMPRIEV DGPFGTVSED VFQYEVAVLV
410 420 430 440 450
GAGIGVTPFA SFLKSIWYKF QRAHNKLKTQ KIYFYWICRE TGAFAWFNNL
460 470 480 490 500
LNSLEQEMDE LGKPDFLNYR LFLTGWDSNI AGHAALNFDR ATDVLTGLKQ
510 520 530 540 550
KTSFGRPMWD NEFSRIATAH PKSVVGVFLC GPPTLAKSLR KCCRRYSSLD
560
PRKVQFYFNK ETF
Length:563
Mass (Da):65,177
Last modified:November 1, 1999 - v1
Checksum:i2B74BCA021CC3335
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF152963 mRNA Translation: AAD39542.1
RefSeqiNP_446135.1, NM_053683.1
UniGeneiRn.220465

Genome annotation databases

GeneIDi114243
KEGGirno:114243

Similar proteinsi

Entry informationi

Entry nameiNOX1_RAT
AccessioniPrimary (citable) accession number: Q9WV87
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: May 23, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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