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Protein

NADPH oxidase 1

Gene

Nox1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H+ ions as part of its electron transport mechanism.

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

The oxidase activity is potentiated by NOXA1 and NOXO1.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi101Iron (heme axial ligand)Curated1
Metal bindingi115Iron (heme axial ligand)Curated1
Metal bindingi208Iron (heme axial ligand)Curated1
Metal bindingi220Iron (heme axial ligand)Curated1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi337 – 343FADSequence analysis7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Transport
LigandFAD, Flavoprotein, Heme, Iron, Metal-binding, NADP

Protein family/group databases

PeroxiBasei5408. RnoNOx01.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH oxidase 1 (EC:1.-.-.-)
Short name:
NOX-1
Alternative name(s):
Mitogenic oxidase 1
Short name:
MOX-1
NADH/NADPH mitogenic oxidase subunit P65-MOX
NOH-1
Gene namesi
Name:Nox1
Synonyms:Mox1, Noh1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620598. Nox1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 31HelicalSequence analysisAdd BLAST23
Topological domaini32 – 44ExtracellularSequence analysisAdd BLAST13
Transmembranei45 – 69HelicalSequence analysisAdd BLAST25
Topological domaini70 – 102CytoplasmicSequence analysisAdd BLAST33
Transmembranei103 – 123HelicalSequence analysisAdd BLAST21
Topological domaini124 – 167ExtracellularSequence analysisAdd BLAST44
Transmembranei168 – 188HelicalSequence analysisAdd BLAST21
Topological domaini189 – 206CytoplasmicSequence analysisAdd BLAST18
Transmembranei207 – 227HelicalSequence analysisAdd BLAST21
Topological domaini228 – 395ExtracellularSequence analysisAdd BLAST168
Transmembranei396 – 416HelicalSequence analysisAdd BLAST21
Topological domaini417 – 563CytoplasmicSequence analysisAdd BLAST147

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075231.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002101491 – 563NADPH oxidase 1Add BLAST563

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi161N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi241N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9WV87.

PTM databases

iPTMnetiQ9WV87.
PhosphoSitePlusiQ9WV87.

Expressioni

Tissue specificityi

Expressed in vascular smooth muscle cells.

Interactioni

Subunit structurei

NOX1, NOXA1, NOXO1, RAC1 and CYBA forms a functional multimeric complex supporting ROS production. Interacts with NOXA1 and NOXO1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

MINTiMINT-4998488.
STRINGi10116.ENSRNOP00000065995.

Structurei

3D structure databases

ProteinModelPortaliQ9WV87.
SMRiQ9WV87.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 282Ferric oxidoreductaseAdd BLAST229
Domaini283 – 390FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST108

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni396 – 535Interaction with NOXO1By similarityAdd BLAST140

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
HOVERGENiHBG003760.
InParanoidiQ9WV87.
KOiK08008.
PhylomeDBiQ9WV87.

Family and domain databases

InterProiView protein in InterPro
IPR000778. Cyt_b245_heavy_chain.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR029650. NOX1.
IPR017938. Riboflavin_synthase-like_b-brl.
PANTHERiPTHR11972:SF71. PTHR11972:SF71. 1 hit.
PfamiView protein in Pfam
PF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiView protein in PROSITE
PS51384. FAD_FR. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9WV87-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNWLVNHWL SVLFLVSWLG LNIFLFVYVF LNYEKSDKYY YTREILGTAL
60 70 80 90 100
ALARASALCL NFNSMVILIP VCRNLLSFLR GTCSFCNHTL RKPLDHNLTF
110 120 130 140 150
HKLVAYMICI FTAIHIIAHL FNFERYSRSQ QAMDGSLASV LSSLFHPEKE
160 170 180 190 200
DSWLNPIQSP NVTVMYAAFT SIAGLTGVVA TVALVLMVTS AMEFIRRNYF
210 220 230 240 250
ELFWYTHHLF IIYIICLGIH GLGGIVRGQT EESMSESHPR NCSYSFHEWD
260 270 280 290 300
KYERSCRSPH FVGQPPESWK WILAPIAFYI FERILRFYRS RQKVVITKVV
310 320 330 340 350
MHPCKVLELQ MRKRGFTMGI GQYIFVNCPS ISFLEWHPFT LTSAPEEEFF
360 370 380 390 400
SIHIRAAGDW TENLIRTFEQ QHSPMPRIEV DGPFGTVSED VFQYEVAVLV
410 420 430 440 450
GAGIGVTPFA SFLKSIWYKF QRAHNKLKTQ KIYFYWICRE TGAFAWFNNL
460 470 480 490 500
LNSLEQEMDE LGKPDFLNYR LFLTGWDSNI AGHAALNFDR ATDVLTGLKQ
510 520 530 540 550
KTSFGRPMWD NEFSRIATAH PKSVVGVFLC GPPTLAKSLR KCCRRYSSLD
560
PRKVQFYFNK ETF
Length:563
Mass (Da):65,177
Last modified:November 1, 1999 - v1
Checksum:i2B74BCA021CC3335
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF152963 mRNA. Translation: AAD39542.1.
RefSeqiNP_446135.1. NM_053683.1.
UniGeneiRn.220465.

Genome annotation databases

GeneIDi114243.
KEGGirno:114243.

Similar proteinsi

Entry informationi

Entry nameiNOX1_RAT
AccessioniPrimary (citable) accession number: Q9WV87
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: November 22, 2017
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome