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Protein

Nucleoside diphosphate kinase, mitochondrial

Gene

Nme4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.

Catalytic activityi

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei44 – 441ATPBy similarity
Binding sitei92 – 921ATPBy similarity
Binding sitei120 – 1201ATPBy similarity
Binding sitei126 – 1261ATPBy similarity
Binding sitei137 – 1371ATPBy similarity
Binding sitei147 – 1471ATPBy similarity
Active sitei150 – 1501Pros-phosphohistidine intermediateBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. nucleoside diphosphate kinase activity Source: MGI

GO - Biological processi

  1. CTP biosynthetic process Source: InterPro
  2. GTP biosynthetic process Source: InterPro
  3. nucleoside triphosphate biosynthetic process Source: GO_Central
  4. purine nucleotide metabolic process Source: GO_Central
  5. pyrimidine nucleotide metabolic process Source: GO_Central
  6. regulation of apoptotic process Source: GO_Central
  7. UTP biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_238368. Synthesis and interconversion of nucleotide di- and triphosphates.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoside diphosphate kinase, mitochondrial (EC:2.7.4.6)
Short name:
NDK
Short name:
NDP kinase, mitochondrial
Alternative name(s):
Nucleoside diphosphate kinase D
Short name:
NDPKD
nm23-M4
Gene namesi
Name:Nme4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:1931148. Nme4.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: GO_Central
  2. mitochondrial intermembrane space Source: UniProtKB-SubCell
  3. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3232MitochondrionSequence AnalysisAdd
BLAST
Chaini33 – 186154Nucleoside diphosphate kinase, mitochondrialPRO_0000019433Add
BLAST

Proteomic databases

MaxQBiQ9WV84.
PaxDbiQ9WV84.
PRIDEiQ9WV84.

PTM databases

PhosphoSiteiQ9WV84.

Expressioni

Tissue specificityi

Expressed in the base region of the oxyntic and pyloric mucosae.1 Publication

Gene expression databases

BgeeiQ9WV84.
CleanExiMM_NME4.
GenevestigatoriQ9WV84.

Interactioni

Subunit structurei

Homohexamer (By similarity). Interacts with CAPN8.By similarity1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9WV84.
SMRiQ9WV84. Positions 36-174.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NDK family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0105.
GeneTreeiENSGT00760000119146.
HOVERGENiHBG000423.
InParanoidiQ9WV84.
KOiK00940.
OMAiMVHLSQE.
OrthoDBiEOG7GJ6FG.
PhylomeDBiQ9WV84.
TreeFamiTF106373.

Family and domain databases

Gene3Di3.30.70.141. 1 hit.
HAMAPiMF_00451. NDP_kinase.
InterProiIPR001564. Nucleoside_diP_kinase.
IPR023005. Nucleoside_diP_kinase_AS.
[Graphical view]
PfamiPF00334. NDK. 1 hit.
[Graphical view]
PRINTSiPR01243. NUCDPKINASE.
SMARTiSM00562. NDK. 1 hit.
[Graphical view]
SUPFAMiSSF54919. SSF54919. 1 hit.
PROSITEiPS00469. NDP_KINASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9WV84-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGSLFGRVAA LRALLCGPRF QCLLVRPSSG GPPWPQERTL VAVKPDGVQR
60 70 80 90 100
RLVGTVIQRF ERRGFKLVGM KMLQAPESIL AEHYRDLQRK PFYPALISYM
110 120 130 140 150
SSGPVVAMVW EGPNVVHISR AMIGHTDSTE AAPGTIRGDF SVHISRNVIH
160 170 180
ASDSVDGAQR EIELWFQSSE LLNWADGGHH SSCYPA
Length:186
Mass (Da):20,549
Last modified:November 1, 1999 - v1
Checksum:i472A32D65C0B25A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF153451 mRNA. Translation: AAD38977.1.
AF288690 mRNA. Translation: AAG02200.1.
AF288692 Genomic DNA. Translation: AAG02202.1.
BC027277 mRNA. Translation: AAH27277.1.
CCDSiCCDS28544.1.
RefSeqiNP_062705.1. NM_019731.1.
UniGeneiMm.41787.

Genome annotation databases

EnsembliENSMUST00000025007; ENSMUSP00000025007; ENSMUSG00000024177.
GeneIDi56520.
KEGGimmu:56520.
UCSCiuc008bdh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF153451 mRNA. Translation: AAD38977.1.
AF288690 mRNA. Translation: AAG02200.1.
AF288692 Genomic DNA. Translation: AAG02202.1.
BC027277 mRNA. Translation: AAH27277.1.
CCDSiCCDS28544.1.
RefSeqiNP_062705.1. NM_019731.1.
UniGeneiMm.41787.

3D structure databases

ProteinModelPortaliQ9WV84.
SMRiQ9WV84. Positions 36-174.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ9WV84.

Proteomic databases

MaxQBiQ9WV84.
PaxDbiQ9WV84.
PRIDEiQ9WV84.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025007; ENSMUSP00000025007; ENSMUSG00000024177.
GeneIDi56520.
KEGGimmu:56520.
UCSCiuc008bdh.1. mouse.

Organism-specific databases

CTDi4833.
MGIiMGI:1931148. Nme4.

Phylogenomic databases

eggNOGiCOG0105.
GeneTreeiENSGT00760000119146.
HOVERGENiHBG000423.
InParanoidiQ9WV84.
KOiK00940.
OMAiMVHLSQE.
OrthoDBiEOG7GJ6FG.
PhylomeDBiQ9WV84.
TreeFamiTF106373.

Enzyme and pathway databases

ReactomeiREACT_238368. Synthesis and interconversion of nucleotide di- and triphosphates.

Miscellaneous databases

NextBioi312846.
PROiQ9WV84.
SOURCEiSearch...

Gene expression databases

BgeeiQ9WV84.
CleanExiMM_NME4.
GenevestigatoriQ9WV84.

Family and domain databases

Gene3Di3.30.70.141. 1 hit.
HAMAPiMF_00451. NDP_kinase.
InterProiIPR001564. Nucleoside_diP_kinase.
IPR023005. Nucleoside_diP_kinase_AS.
[Graphical view]
PfamiPF00334. NDK. 1 hit.
[Graphical view]
PRINTSiPR01243. NUCDPKINASE.
SMARTiSM00562. NDK. 1 hit.
[Graphical view]
SUPFAMiSSF54919. SSF54919. 1 hit.
PROSITEiPS00469. NDP_KINASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Mehus J.G., Lambeth D.O.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Characterization of the nm23-M2, nm23-M3 and nm23-M4 mouse genes: comparison with their human orthologs."
    Masse K., Dabernat S., Bourbon P.-M., Larou M., Amrein L., Barraud P., Perel Y., Camara M., Landry M., Lacombe M.L., Daniel J.-Y.
    Gene 296:87-97(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary gland.
  4. "Stomach-specific calpain, nCL-2, localizes in mucus cells and proteolyzes the beta-subunit of coatomer complex, beta-COP."
    Hata S., Koyama S., Kawahara H., Doi N., Maeda T., Toyama-Sorimachi N., Abe K., Suzuki K., Sorimachi H.
    J. Biol. Chem. 281:11214-11224(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CAPN8, TISSUE SPECIFICITY.

Entry informationi

Entry nameiNDKM_MOUSE
AccessioniPrimary (citable) accession number: Q9WV84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1999
Last modified: February 4, 2015
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.