Reviewed, UniProtKB/Swiss-Prot Q9WV84 (NDKM_MOUSE)

Names and origin

Protein names

Recommended name:
    Nucleoside diphosphate kinase, mitochondrial
      Short name=NDP kinase, mitochondrial
      Short name=NDK
    EC=2.7.4.6
Alternative name(s):
    nm23-M4
    Nucleoside diphosphate kinase D
      Short name=NDPKD

Gene names

Name:

Nme4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	186 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.
Cofactor	Magnesium By similarity.
Subunit structure	Homohexamer By similarity. Interacts with CAPN8.
Subcellular location	Mitochondrion intermembrane space By similarity.
Tissue specificity	Expressed in the base region of the oxyntic and pyloric mucosae. Ref.4
Sequence similarities	Belongs to the NDK family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	CTP biosynthetic process Inferred from electronic annotation. Source: InterPro GTP biosynthetic process Inferred from electronic annotation. Source: InterPro UTP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial intermembrane space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 32	32	Mitochondrion Potential
	Chain	33 – 186	154	Nucleoside diphosphate kinase, mitochondrial		PRO_0000019433
Sites
	Active site	150	1	Pros-phosphohistidine intermediate By similarity
	Binding site	44	1	ATP By similarity
	Binding site	92	1	ATP By similarity
	Binding site	120	1	ATP By similarity
	Binding site	126	1	ATP By similarity
	Binding site	137	1	ATP By similarity
	Binding site	147	1	ATP By similarity
Amino acid modifications
	Modified residue	90	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9WV84-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 472A32D65C0B25A0	FASTA	186	20,549
	10 20 30 40 50 60 MGSLFGRVAA LRALLCGPRF QCLLVRPSSG GPPWPQERTL VAVKPDGVQR RLVGTVIQRF 70 80 90 100 110 120 ERRGFKLVGM KMLQAPESIL AEHYRDLQRK PFYPALISYM SSGPVVAMVW EGPNVVHISR 130 140 150 160 170 180 AMIGHTDSTE AAPGTIRGDF SVHISRNVIH ASDSVDGAQR EIELWFQSSE LLNWADGGHH SSCYPA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Mehus J.G., Lambeth D.O. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Characterization of the nm23-M2, nm23-M3 and nm23-M4 mouse genes: comparison with their human orthologs." Masse K., Dabernat S., Bourbon P.-M., Larou M., Amrein L., Barraud P., Perel Y., Camara M., Landry M., Lacombe M.L., Daniel J.-Y. Gene 296:87-97(2002) [PubMed: 12383506] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. Tissue: Mammary gland.
[4]	"Stomach-specific calpain, nCL-2, localizes in mucus cells and proteolyzes the beta-subunit of coatomer complex, beta-COP." Hata S., Koyama S., Kawahara H., Doi N., Maeda T., Toyama-Sorimachi N., Abe K., Suzuki K., Sorimachi H. J. Biol. Chem. 281:11214-11224(2006) [PubMed: 16476741] [Abstract] Cited for: INTERACTION WITH CAPN8, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF153451 mRNA. Translation: AAD38977.1. AF288690 mRNA. Translation: AAG02200.1. AF288692 Genomic DNA. Translation: AAG02202.1. BC027277 mRNA. Translation: AAH27277.1.
IPI	IPI00125448.
RefSeq	NP_062705.1.
UniGene	Mm.41787
3D structure databases
HSSP	HSSP built from PDB template 1EHW based on UniProtKB O00746.
SMR	Q9WV84. Positions 36-174.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9WV84.
Proteomic databases
PRIDE	Q9WV84.
Genome annotation databases
Ensembl	ENSMUST00000025007; ENSMUSP00000025007; ENSMUSG00000024177; Mus musculus. [Genome view]
GeneID	56520.
KEGG	mmu:56520.
NMPDR	fig|10090.3.peg.2430.
UCSC	uc008bdh.1. mouse.
Organism-specific databases
CTD	56520.
MGI	MGI:1931148. Nme4.
Phylogenomic databases
HOVERGEN	Q9WV84.
OMA	GKEFYER.
Enzyme and pathway databases
BRENDA	2.7.4.6. 244.
Gene expression databases
ArrayExpress	Q9WV84.
Bgee	Q9WV84.
CleanEx	MM_NME4.
Genevestigator	Q9WV84.
GermOnline	ENSMUSG00000024177. Mus musculus.
Family and domain databases
InterPro	IPR001564. Nuc_diP_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.70.141. NDK. 1 hit.
PANTHER	PTHR11349. Nuc_diP_kinase_core. 1 hit.
Pfam	PF00334. NDK. 1 hit. [Graphical view]
PRINTS	PR01243. NUCDPKINASE.
ProDom	PD001018. NDK. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00562. NDK. 1 hit. [Graphical view]
PROSITE	PS00469. NDP_KINASES. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	312846.
SOURCE	Search...

Entry information

Entry name

NDKM_MOUSE

Accession

Primary (citable) accession number: Q9WV84

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	November 1, 1999
Last modified:	November 3, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)