Sorting nexin-1 - Mus musculus (Mouse)

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Q9WV80 (SNX1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified October 19, 2011. Version 92. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Sorting nexin-1

Gene names

Name:

Snx1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	522 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in several stages of intracellular trafficking. Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) By similarity.
Subunit structure	Self-assembles into a complex of approximately 300 kDa. Interacts with HGS. Component of the retromer complex composed of VPS26 (VPS26A or VPS26B), VPS29, VPS35, SNX1 and SNX2. Interacts with SNX6 By similarity. Ref.2
Subcellular location	Endosome membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Golgi apparatus › trans-Golgi network membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Note: Enriched on tubular elements of the early endosome membrane. Binds preferentially to highly curved membranes enriched in phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) By similarity.
Disruption phenotype	No visible phenotype. Mice are born at the expected Mendelian ratio and are fertile. Mice lacking both Snx1 and Snx2 die during embryonic development, around 9.5 and 11.5 dpc. Ref.3
Miscellaneous	Binds phosphatidylinositol 3-phosphate (PtdIns-₃P) and phosphatidylinositol 3,5-bisphosphate (PtdIns-(3,5)P2) in liposome-based assays. Can bind PtdIns(3,4,5)P3 in protein:lipid overlay assays, but not in liposome-based assays By similarity.
Sequence similarities	Belongs to the sorting nexin family. Contains 1 BAR domain. Contains 1 PX (phox homology) domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Endosome Golgi apparatus Membrane
Ligand	Lipid-binding
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell communication Inferred from electronic annotation. Source: InterPro intracellular protein transport Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell early endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphatidylinositol binding Inferred from sequence or structural similarity. Source: UniProtKB protein transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 522

522

Sorting nexin-1

PRO_0000213836

Regions

Domain

143 – 272

130

Domain

302 – 522

221

BAR

Sites

Binding site

186

Phosphatidylinositol 3-phosphate By similarity

Binding site

188

Phosphatidylinositol 3-phosphate; via amide nitrogen and carbonyl oxygen By similarity

Binding site

214

Phosphatidylinositol 3-phosphate By similarity

Binding site

238

Phosphatidylinositol 3-phosphate By similarity

Amino acid modifications

Modified residue

Phosphoserine By similarity

Modified residue

Phosphoserine By similarity

Modified residue

Phosphothreonine By similarity

Modified residue

188

Phosphoserine Ref.4

Modified residue

237

N6-acetyllysine By similarity

Experimental info

Sequence conflict

A → G in BAB20283. Ref.2

Sequence conflict

117

S → P in BAB20283. Ref.2

Sequence conflict

124

S → C in BAB20283. Ref.2

Sequence conflict

138

Missing in BAB20283. Ref.2

Sequence conflict

389

S → F in BAB20283. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q9WV80 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 1EFC06B3EA551311
Show »

FASTA

522

58,952

        10         20         30         40         50         60 
MASGGGGCSA SERLPPPFPG MDPESEGAAG ASEPEAGDSD TEGEDIFTGA AAATKPQSPK 

        70         80         90        100        110        120 
KTTSLFPIKN GSKENGIHED QDQEPQDLFA DATVELSLDS TQNNQKTMPG KTLTSHSPQE 

       130        140        150        160        170        180 
ATNSPKPQPS YEELEEEEQE DQFDLTVGIT DPEKIGDGMN AYVAYKVTTQ TSLPMFRSRQ 

       190        200        210        220        230        240 
FAVKRRFSDF LGLYEKLSEK HSQNGFIVPP PPEKSLIGMT KVKVGKEDSS SAEFLEKRRA 

       250        260        270        280        290        300 
ALERYLQRIV NHPTMLQDPD VREFLEKEEL PRAVGTQALS GAGLLKMFNK ATDAVSKMTI 

       310        320        330        340        350        360 
KMNESDIWFE EKLQEVECEE QRLRKLHAVV ETLVNHRKEL ALNTALFAKS LAMLGSSEDN 

       370        380        390        400        410        420 
TALSRALSQL AEVEEKIEQL HQEQANNDSF LLAELLSDYI RLLAIVRAAF DQRMKTWQRW 

       430        440        450        460        470        480 
QDAQATLQKK RESEARLLWA NKPDKLQQAK DEITEWESRV TQYERDFERI STVVRKEVTR 

       490        500        510        520 
FEKEKSKDFK NHVMKYLETL LHSQQQLAKY WEAFLPEAKA IS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence of mouse sorting nexin 1 (Snx1) cDNA." Takahara K., Omatsu Y., Maeda Y., Shimoyama S., Inaba K. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Thymus.
[2]	"Association of mouse sorting nexin 1 with early endosomes." Nakamura N., Sun-Wada G.H., Yamamoto A., Wada Y., Futai M. J. Biochem. 130:765-771(2001) [PubMed: 11726276] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT. Strain: C57BL/6.
[3]	"Genetic analysis of sorting nexins 1 and 2 reveals a redundant and essential function in mice." Schwarz D.G., Griffin C.T., Schneider E.A., Yee D., Magnuson T. Mol. Biol. Cell 13:3588-3600(2002) [PubMed: 12388759] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[4]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed: 19367708] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-188, MASS SPECTROMETRY. Tissue: Melanoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF154120 mRNA. Translation: AAD38805.1. AB019214 mRNA. Translation: BAB20283.1.
IPI	IPI00622364.
RefSeq	NP_062701.2. NM_019727.2.
UniGene	Mm.271891.
3D structure databases
ProteinModelPortal	Q9WV80.
SMR	Q9WV80. Positions 142-269.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9WV80. 1 interaction.
STRING	Q9WV80.
PTM databases
PhosphoSite	Q9WV80.
Proteomic databases
PRIDE	Q9WV80.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	56440.
KEGG	mmu:56440.
Organism-specific databases
CTD	6642.
MGI	MGI:1928395. Snx1.
Phylogenomic databases
GeneTree	ENSGT00550000074512.
HOGENOM	HBG445848.
HOVERGEN	HBG000618.
InParanoid	Q9WV80.
OrthoDB	EOG418BNH.
PhylomeDB	Q9WV80.
Gene expression databases
ArrayExpress	Q9WV80.
Bgee	Q9WV80.
CleanEx	MM_SNX1.
Genevestigator	Q9WV80.
GermOnline	ENSMUSG00000032382. Mus musculus.
Family and domain databases
InterPro	IPR001683. Phox. IPR005329. Sorting_nexin_N. IPR015404. Vps5_C. [Graphical view]
Gene3D	G3DSA:3.30.1520.10. PX. 1 hit.
Pfam	PF00787. PX. 1 hit. PF03700. Sorting_nexin. 1 hit. PF09325. Vps5. 1 hit. [Graphical view]
SMART	SM00312. PX. 1 hit. [Graphical view]
SUPFAM	SSF64268. PX. 1 hit.
PROSITE	PS51021. BAR. False negative. PS50195. PX. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	312630.
SOURCE	Search...

Entry information

Entry name

SNX1_MOUSE

Accession

Primary (citable) accession number: Q9WV80
Secondary accession number(s): Q9EQZ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	November 1, 1999
Last modified:	October 19, 2011
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents