Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9WV80 (SNX1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sorting nexin-1
Gene names
Name:Snx1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length522 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in several stages of intracellular trafficking. Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) By similarity.

Subunit structure

Self-assembles into a complex of approximately 300 kDa. Interacts with HGS. Component of the retromer complex composed of VPS26 (VPS26A or VPS26B), VPS29, VPS35, SNX1 and SNX2. Interacts with SNX6 By similarity. Ref.2

Subcellular location

Endosome membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Golgi apparatustrans-Golgi network membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Note: Enriched on tubular elements of the early endosome membrane. Binds preferentially to highly curved membranes enriched in phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) By similarity.

Disruption phenotype

No visible phenotype. Mice are born at the expected Mendelian ratio and are fertile. Mice lacking both Snx1 and Snx2 die during embryonic development, around 9.5 and 11.5 dpc. Ref.3

Miscellaneous

Binds phosphatidylinositol 3-phosphate (PtdIns-3P) and phosphatidylinositol 3,5-bisphosphate (PtdIns-(3,5)P2) in liposome-based assays. Can bind PtdIns(3,4,5)P3 in protein:lipid overlay assays, but not in liposome-based assays By similarity.

Sequence similarities

Belongs to the sorting nexin family.

Contains 1 BAR domain.

Contains 1 PX (phox homology) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 522522Sorting nexin-1
PRO_0000213836

Regions

Domain143 – 272130PX
Domain302 – 522221BAR

Sites

Binding site1861Phosphatidylinositol 3-phosphate By similarity
Binding site1881Phosphatidylinositol 3-phosphate; via amide nitrogen and carbonyl oxygen By similarity
Binding site2141Phosphatidylinositol 3-phosphate By similarity
Binding site2381Phosphatidylinositol 3-phosphate By similarity

Amino acid modifications

Modified residue321Phosphoserine By similarity
Modified residue391Phosphoserine By similarity
Modified residue1881Phosphoserine Ref.4
Modified residue2371N6-acetyllysine By similarity

Experimental info

Sequence conflict311A → G in BAB20283. Ref.2
Sequence conflict1171S → P in BAB20283. Ref.2
Sequence conflict1241S → C in BAB20283. Ref.2
Sequence conflict1381Missing in BAB20283. Ref.2
Sequence conflict3891S → F in BAB20283. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9WV80 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 1EFC06B3EA551311

FASTA52258,952
        10         20         30         40         50         60 
MASGGGGCSA SERLPPPFPG MDPESEGAAG ASEPEAGDSD TEGEDIFTGA AAATKPQSPK 

        70         80         90        100        110        120 
KTTSLFPIKN GSKENGIHED QDQEPQDLFA DATVELSLDS TQNNQKTMPG KTLTSHSPQE 

       130        140        150        160        170        180 
ATNSPKPQPS YEELEEEEQE DQFDLTVGIT DPEKIGDGMN AYVAYKVTTQ TSLPMFRSRQ 

       190        200        210        220        230        240 
FAVKRRFSDF LGLYEKLSEK HSQNGFIVPP PPEKSLIGMT KVKVGKEDSS SAEFLEKRRA 

       250        260        270        280        290        300 
ALERYLQRIV NHPTMLQDPD VREFLEKEEL PRAVGTQALS GAGLLKMFNK ATDAVSKMTI 

       310        320        330        340        350        360 
KMNESDIWFE EKLQEVECEE QRLRKLHAVV ETLVNHRKEL ALNTALFAKS LAMLGSSEDN 

       370        380        390        400        410        420 
TALSRALSQL AEVEEKIEQL HQEQANNDSF LLAELLSDYI RLLAIVRAAF DQRMKTWQRW 

       430        440        450        460        470        480 
QDAQATLQKK RESEARLLWA NKPDKLQQAK DEITEWESRV TQYERDFERI STVVRKEVTR 

       490        500        510        520 
FEKEKSKDFK NHVMKYLETL LHSQQQLAKY WEAFLPEAKA IS 

« Hide

References

« Hide 'large scale' references
[1]"Complete sequence of mouse sorting nexin 1 (Snx1) cDNA."
Takahara K., Omatsu Y., Maeda Y., Shimoyama S., Inaba K.
Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Thymus.
[2]"Association of mouse sorting nexin 1 with early endosomes."
Nakamura N., Sun-Wada G.H., Yamamoto A., Wada Y., Futai M.
J. Biochem. 130:765-771(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT.
Strain: C57BL/6.
[3]"Genetic analysis of sorting nexins 1 and 2 reveals a redundant and essential function in mice."
Schwarz D.G., Griffin C.T., Schneider E.A., Yee D., Magnuson T.
Mol. Biol. Cell 13:3588-3600(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[4]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-188, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF154120 mRNA. Translation: AAD38805.1.
AB019214 mRNA. Translation: BAB20283.1.
RefSeqNP_062701.2. NM_019727.2.
UniGeneMm.271891.

3D structure databases

ProteinModelPortalQ9WV80.
SMRQ9WV80. Positions 142-269, 305-513.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid207981. 1 interaction.
IntActQ9WV80. 2 interactions.
MINTMINT-1864956.

PTM databases

PhosphoSiteQ9WV80.

Proteomic databases

MaxQBQ9WV80.
PaxDbQ9WV80.
PRIDEQ9WV80.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID56440.
KEGGmmu:56440.

Organism-specific databases

CTD6642.
MGIMGI:1928395. Snx1.

Phylogenomic databases

eggNOGCOG5391.
HOGENOMHOG000293327.
HOVERGENHBG000618.
InParanoidQ9WV80.
KOK17917.
PhylomeDBQ9WV80.

Gene expression databases

CleanExMM_SNX1.
GenevestigatorQ9WV80.

Family and domain databases

Gene3D3.30.1520.10. 1 hit.
InterProIPR001683. Phox.
IPR028660. SNX1.
IPR005329. Sorting_nexin_N.
IPR015404. Vps5_C.
[Graphical view]
PANTHERPTHR10555:SF129. PTHR10555:SF129. 1 hit.
PfamPF00787. PX. 1 hit.
PF03700. Sorting_nexin. 1 hit.
PF09325. Vps5. 1 hit.
[Graphical view]
SMARTSM00312. PX. 1 hit.
[Graphical view]
SUPFAMSSF64268. SSF64268. 1 hit.
PROSITEPS50195. PX. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSNX1. mouse.
NextBio312630.
PROQ9WV80.
SOURCESearch...

Entry information

Entry nameSNX1_MOUSE
AccessionPrimary (citable) accession number: Q9WV80
Secondary accession number(s): Q9EQZ9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1999
Last modified: July 9, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot