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Protein

AP-4 complex subunit beta-1

Gene

Ap4b1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of novel type of clathrin- or non-clathrin-associated protein coat involved in targeting proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system.By similarity

GO - Molecular functioni

GO - Biological processi

  • intracellular protein transport Source: MGI
  • vesicle-mediated transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-432720. Lysosome Vesicle Biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-4 complex subunit beta-1
Alternative name(s):
AP-4 adaptor complex subunit beta
Adaptor-related protein complex 4 subunit beta-1
Beta subunit of AP-4
Beta4-adaptin
Gene namesi
Name:Ap4b1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1337130. Ap4b1.

Subcellular locationi

GO - Cellular componenti

  • clathrin adaptor complex Source: InterPro
  • coated pit Source: UniProtKB-SubCell
  • trans-Golgi network Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Coated pit, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 738738AP-4 complex subunit beta-1PRO_0000193751Add
BLAST

Proteomic databases

EPDiQ9WV76.
MaxQBiQ9WV76.
PaxDbiQ9WV76.
PRIDEiQ9WV76.

PTM databases

PhosphoSiteiQ9WV76.

Expressioni

Gene expression databases

CleanExiMM_AP4B1.
ExpressionAtlasiQ9WV76. baseline.
GenevisibleiQ9WV76. MM.

Interactioni

Subunit structurei

Adaptor protein complex 4 (AP-4) is a heterotetramer composed of two large adaptins (epsilon-type subunit AP4E1 and beta-type subunit AP4B1), a medium adaptin (mu-type subunit AP4M1) and a small adaptin (sigma-type AP4S1). Interacts with ENTHD2; this interaction requires the presence of a functional AP-4 complex (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044262.

Structurei

3D structure databases

ProteinModelPortaliQ9WV76.
SMRiQ9WV76. Positions 12-537, 612-738.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1061. Eukaryota.
COG5096. LUCA.
GeneTreeiENSGT00530000063138.
HOGENOMiHOG000294128.
HOVERGENiHBG050521.
InParanoidiQ9WV76.
KOiK12401.
OMAiLNRMPKL.
OrthoDBiEOG7DJSKJ.
TreeFamiTF354235.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
3.30.310.10. 1 hit.
InterProiIPR026739. AP_beta.
IPR016342. AP_complex_bsu_1_2_4.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR015151. B-adaptin_app_sub_C.
IPR012295. Beta2_adaptin/TBP_C_dom.
IPR002553. Clathrin/coatomer_adapt-like_N.
[Graphical view]
PANTHERiPTHR11134. PTHR11134. 1 hit.
PfamiPF01602. Adaptin_N. 1 hit.
PF09066. B2-adapt-app_C. 1 hit.
[Graphical view]
PIRSFiPIRSF002291. AP_complex_beta. 1 hit.
SMARTiSM01020. B2-adapt-app_C. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9WV76-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPYLGSEDVV KELKKALCNP HIQADRLRYR NVIQRVIRHM TQGLDMSDVF
60 70 80 90 100
MEMVKASATV DIVQKKLVYL YMGTYAPLKP DLALLAINTL CKDCSDPNPM
110 120 130 140 150
VRGLALRSMC SLRMPGVQEY IQQPVVNGLR DKASYVRRVA VLGCAKMHNL
160 170 180 190 200
HGDSEVDGAL VNELYSLLRD QDPIVVVNCL RSLEEILKQE GGVVINKPIA
210 220 230 240 250
HHLLNRMSKL DQWGQAEVLN FLLRYQPRSE EELFDILNLL DSYLKSSSTG
260 270 280 290 300
VVMGATKLFL ILAKKFPHVQ TDVLVRVKGP LLAACSSESR ELCFAALCHV
310 320 330 340 350
RQVLHSLPGH FSSHYKKFFC SYSEPHYIKL QKVEVLCELV NDENVQQVLE
360 370 380 390 400
ELRGYCTDVA ADFAQAAIFA IGSIAKTYTD QCVQILTELL GLRQEHITTV
410 420 430 440 450
VVQTFRDLVW LCPQCTEAVC QALPGCEENI QDSEGKQALI WLLGVHGEKI
460 470 480 490 500
PNAPYVLEDF VDNVKSETFP AVKMELLTAL MRLVLSRPAE CQDMLGRLLH
510 520 530 540 550
YCIEEEKDMA VRDRGLFYYR LLLVGIDKVK QILCSPKSDP SLGLLEDQPE
560 570 580 590 600
RPVNSWASDF NTLAPVYGRA HWATISKCQQ VERHRLELPH NASFATSGHL
610 620 630 640 650
ISEENKEGAQ EPDSDTLMLV PNLQLTAEYF EKTWLSLRVS YQQVFPWQGE
660 670 680 690 700
VQPDTLQMAL KVVNIQTIAM SRAGAQPWKA YLSAQDDTGG LFLAELLLKP
710 720 730
ENSEMQISVK QSKARTESLH GFVSVLETVI GTVGDIKS
Length:738
Mass (Da):82,976
Last modified:October 3, 2012 - v2
Checksum:i1F05223F89E1A360
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti228 – 2281R → L in AAD43327 (PubMed:10436028).Curated
Sequence conflicti360 – 3601A → S in AAD43327 (PubMed:10436028).Curated
Sequence conflicti373 – 3731S → N in AAD43327 (PubMed:10436028).Curated
Sequence conflicti462 – 4632DN → QS in AAD43327 (PubMed:10436028).Curated
Sequence conflicti484 – 4841V → F in AAD43327 (PubMed:10436028).Curated
Sequence conflicti494 – 4941M → V in AAD43327 (PubMed:10436028).Curated
Sequence conflicti500 – 5001H → L in AAD43327 (PubMed:10436028).Curated
Sequence conflicti504 – 5041E → Q in AAD43327 (PubMed:10436028).Curated
Sequence conflicti529 – 5291V → T in AAD43327 (PubMed:10436028).Curated
Sequence conflicti545 – 5451L → S in AAD43327 (PubMed:10436028).Curated
Sequence conflicti569 – 5691R → K in AAD43327 (PubMed:10436028).Curated
Sequence conflicti580 – 5834QVER → KIEP in AAD43327 (PubMed:10436028).Curated
Sequence conflicti602 – 6021S → A in AAD43327 (PubMed:10436028).Curated
Sequence conflicti611 – 6111E → EP in AAD43327 (PubMed:10436028).Curated
Sequence conflicti615 – 6184DTLM → GALV in AAD43327 (PubMed:10436028).Curated
Sequence conflicti629 – 6291Y → C in AAD43327 (PubMed:10436028).Curated
Sequence conflicti638 – 6381R → T in AAD43327 (PubMed:10436028).Curated
Sequence conflicti642 – 6421Q → H in AAD43327 (PubMed:10436028).Curated
Sequence conflicti645 – 6451F → L in AAD43327 (PubMed:10436028).Curated
Sequence conflicti672 – 6732RA → KP in AAD43327 (PubMed:10436028).Curated
Sequence conflicti687 – 6871D → G in AAD43327 (PubMed:10436028).Curated
Sequence conflicti694 – 6941A → T in AAD43327 (PubMed:10436028).Curated
Sequence conflicti705 – 7051M → V in AAD43327 (PubMed:10436028).Curated
Sequence conflicti713 – 7131K → E in AAD43327 (PubMed:10436028).Curated
Sequence conflicti718 – 7203SLH → ALR in AAD43327 (PubMed:10436028).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF155157 mRNA. Translation: AAD43327.1.
AC124698 Genomic DNA. No translation available.
CU210953 Genomic DNA. Translation: CAQ12171.1.
CCDSiCCDS17696.1.
RefSeqiNP_001157024.1. NM_001163552.1.
NP_080469.2. NM_026193.2.
UniGeneiMm.197568.

Genome annotation databases

EnsembliENSMUST00000047285; ENSMUSP00000044262; ENSMUSG00000032952.
ENSMUST00000076599; ENSMUSP00000075904; ENSMUSG00000032952.
GeneIDi67489.
KEGGimmu:67489.
UCSCiuc008qtq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF155157 mRNA. Translation: AAD43327.1.
AC124698 Genomic DNA. No translation available.
CU210953 Genomic DNA. Translation: CAQ12171.1.
CCDSiCCDS17696.1.
RefSeqiNP_001157024.1. NM_001163552.1.
NP_080469.2. NM_026193.2.
UniGeneiMm.197568.

3D structure databases

ProteinModelPortaliQ9WV76.
SMRiQ9WV76. Positions 12-537, 612-738.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044262.

PTM databases

PhosphoSiteiQ9WV76.

Proteomic databases

EPDiQ9WV76.
MaxQBiQ9WV76.
PaxDbiQ9WV76.
PRIDEiQ9WV76.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047285; ENSMUSP00000044262; ENSMUSG00000032952.
ENSMUST00000076599; ENSMUSP00000075904; ENSMUSG00000032952.
GeneIDi67489.
KEGGimmu:67489.
UCSCiuc008qtq.2. mouse.

Organism-specific databases

CTDi10717.
MGIiMGI:1337130. Ap4b1.

Phylogenomic databases

eggNOGiKOG1061. Eukaryota.
COG5096. LUCA.
GeneTreeiENSGT00530000063138.
HOGENOMiHOG000294128.
HOVERGENiHBG050521.
InParanoidiQ9WV76.
KOiK12401.
OMAiLNRMPKL.
OrthoDBiEOG7DJSKJ.
TreeFamiTF354235.

Enzyme and pathway databases

ReactomeiR-MMU-432720. Lysosome Vesicle Biogenesis.

Miscellaneous databases

ChiTaRSiAp4b1. mouse.
NextBioi324726.
PROiQ9WV76.
SOURCEiSearch...

Gene expression databases

CleanExiMM_AP4B1.
ExpressionAtlasiQ9WV76. baseline.
GenevisibleiQ9WV76. MM.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
3.30.310.10. 1 hit.
InterProiIPR026739. AP_beta.
IPR016342. AP_complex_bsu_1_2_4.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR015151. B-adaptin_app_sub_C.
IPR012295. Beta2_adaptin/TBP_C_dom.
IPR002553. Clathrin/coatomer_adapt-like_N.
[Graphical view]
PANTHERiPTHR11134. PTHR11134. 1 hit.
PfamiPF01602. Adaptin_N. 1 hit.
PF09066. B2-adapt-app_C. 1 hit.
[Graphical view]
PIRSFiPIRSF002291. AP_complex_beta. 1 hit.
SMARTiSM01020. B2-adapt-app_C. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a fourth adaptor-related protein complex."
    Hirst J., Bright N.A., Rous B., Robinson M.S.
    Mol. Biol. Cell 10:2787-2802(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Lung, Spleen and Testis.

Entry informationi

Entry nameiAP4B1_MOUSE
AccessioniPrimary (citable) accession number: Q9WV76
Secondary accession number(s): B0V3P2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: October 3, 2012
Last modified: March 16, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.