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Protein

AP-4 complex subunit beta-1

Gene

Ap4b1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the adaptor protein complex 4 (AP-4). Adaptor protein complexes are vesicle coat components involved both in vesicle formation and cargo selection. They control the vesicular transport of proteins in different trafficking pathways. AP-4 forms a non clathrin-associated coat on vesicles departing the trans-Golgi network (TGN) and may be involved in the targeting of proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system (By similarity). It is also involved in protein sorting to the basolateral membrane in epithelial cells and the proper asymmetric localization of somatodendritic proteins in neurons (PubMed:18341993). AP-4 is involved in the recognition and binding of tyrosine-based sorting signals found in the cytoplasmic part of cargos, but may also recognize other types of sorting signal (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • intracellular protein transport Source: MGI
  • protein localization to somatodendritic compartment Source: UniProtKB
  • protein targeting Source: UniProtKB
  • vesicle-mediated transport Source: MGI

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-432720. Lysosome Vesicle Biogenesis.
R-MMU-432722. Golgi Associated Vesicle Biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-4 complex subunit beta-1Curated
Alternative name(s):
AP-4 adaptor complex subunit beta
Adaptor-related protein complex 4 subunit beta-1
Beta subunit of AP-4
Beta4-adaptin
Gene namesi
Name:Ap4b1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1337130. Ap4b1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice lacking Ap4b1 are fertile, have no overt anatomical abnormalities and have normal life spans. They only show a significantly poorer rotorod performance than wild-type mice. No significant differences in body weight or grip power is observed compared to wild-type mice. The cerebella has normal foliation and a normal laminated cortical structure. Main neuronal types are present in the cerebella and the gross morphology of the soma and dendrites of Purkinje cells is normal.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001937511 – 738AP-4 complex subunit beta-1Add BLAST738

Proteomic databases

MaxQBiQ9WV76.
PaxDbiQ9WV76.
PRIDEiQ9WV76.

PTM databases

iPTMnetiQ9WV76.
PhosphoSitePlusiQ9WV76.

Expressioni

Gene expression databases

BgeeiENSMUSG00000032952.
CleanExiMM_AP4B1.
ExpressionAtlasiQ9WV76. baseline and differential.
GenevisibleiQ9WV76. MM.

Interactioni

Subunit structurei

Adaptor protein complex 4 (AP-4) is a heterotetramer composed of two large adaptins (epsilon-type subunit AP4E1 and beta-type subunit AP4B1), a medium adaptin (mu-type subunit AP4M1) and a small adaptin (sigma-type AP4S1). Interacts with TEPSIN; this interaction requires the presence of a functional AP-4 complex (By similarity). Interacts with GRIA2; probably indirect it mediates the somatodendritic localization of GRIA2 in neurons (PubMed:18341993).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044262.

Structurei

3D structure databases

ProteinModelPortaliQ9WV76.
SMRiQ9WV76.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni534 – 600HingeBy similarityAdd BLAST67
Regioni601 – 738Ear; mediates interaction with TEPSINBy similarityAdd BLAST138

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1061. Eukaryota.
COG5096. LUCA.
GeneTreeiENSGT00530000063138.
HOGENOMiHOG000294128.
HOVERGENiHBG050521.
InParanoidiQ9WV76.
KOiK12401.
OMAiMSNNDMV.
OrthoDBiEOG091G058E.
TreeFamiTF354235.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
3.30.310.10. 1 hit.
InterProiView protein in InterPro
IPR026739. AP_beta.
IPR016342. AP_complex_bsu_1_2_4.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR015151. B-adaptin_app_sub_C.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR012295. TBP_dom_sf.
PANTHERiPTHR11134. PTHR11134. 1 hit.
PfamiView protein in Pfam
PF01602. Adaptin_N. 1 hit.
PF09066. B2-adapt-app_C. 1 hit.
PIRSFiPIRSF002291. AP_complex_beta. 1 hit.
SMARTiView protein in SMART
SM01020. B2-adapt-app_C. 1 hit.
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9WV76-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPYLGSEDVV KELKKALCNP HIQADRLRYR NVIQRVIRHM TQGLDMSDVF
60 70 80 90 100
MEMVKASATV DIVQKKLVYL YMGTYAPLKP DLALLAINTL CKDCSDPNPM
110 120 130 140 150
VRGLALRSMC SLRMPGVQEY IQQPVVNGLR DKASYVRRVA VLGCAKMHNL
160 170 180 190 200
HGDSEVDGAL VNELYSLLRD QDPIVVVNCL RSLEEILKQE GGVVINKPIA
210 220 230 240 250
HHLLNRMSKL DQWGQAEVLN FLLRYQPRSE EELFDILNLL DSYLKSSSTG
260 270 280 290 300
VVMGATKLFL ILAKKFPHVQ TDVLVRVKGP LLAACSSESR ELCFAALCHV
310 320 330 340 350
RQVLHSLPGH FSSHYKKFFC SYSEPHYIKL QKVEVLCELV NDENVQQVLE
360 370 380 390 400
ELRGYCTDVA ADFAQAAIFA IGSIAKTYTD QCVQILTELL GLRQEHITTV
410 420 430 440 450
VVQTFRDLVW LCPQCTEAVC QALPGCEENI QDSEGKQALI WLLGVHGEKI
460 470 480 490 500
PNAPYVLEDF VDNVKSETFP AVKMELLTAL MRLVLSRPAE CQDMLGRLLH
510 520 530 540 550
YCIEEEKDMA VRDRGLFYYR LLLVGIDKVK QILCSPKSDP SLGLLEDQPE
560 570 580 590 600
RPVNSWASDF NTLAPVYGRA HWATISKCQQ VERHRLELPH NASFATSGHL
610 620 630 640 650
ISEENKEGAQ EPDSDTLMLV PNLQLTAEYF EKTWLSLRVS YQQVFPWQGE
660 670 680 690 700
VQPDTLQMAL KVVNIQTIAM SRAGAQPWKA YLSAQDDTGG LFLAELLLKP
710 720 730
ENSEMQISVK QSKARTESLH GFVSVLETVI GTVGDIKS
Length:738
Mass (Da):82,976
Last modified:October 3, 2012 - v2
Checksum:i1F05223F89E1A360
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti228R → L in AAD43327 (PubMed:10436028).Curated1
Sequence conflicti360A → S in AAD43327 (PubMed:10436028).Curated1
Sequence conflicti373S → N in AAD43327 (PubMed:10436028).Curated1
Sequence conflicti462 – 463DN → QS in AAD43327 (PubMed:10436028).Curated2
Sequence conflicti484V → F in AAD43327 (PubMed:10436028).Curated1
Sequence conflicti494M → V in AAD43327 (PubMed:10436028).Curated1
Sequence conflicti500H → L in AAD43327 (PubMed:10436028).Curated1
Sequence conflicti504E → Q in AAD43327 (PubMed:10436028).Curated1
Sequence conflicti529V → T in AAD43327 (PubMed:10436028).Curated1
Sequence conflicti545L → S in AAD43327 (PubMed:10436028).Curated1
Sequence conflicti569R → K in AAD43327 (PubMed:10436028).Curated1
Sequence conflicti580 – 583QVER → KIEP in AAD43327 (PubMed:10436028).Curated4
Sequence conflicti602S → A in AAD43327 (PubMed:10436028).Curated1
Sequence conflicti611E → EP in AAD43327 (PubMed:10436028).Curated1
Sequence conflicti615 – 618DTLM → GALV in AAD43327 (PubMed:10436028).Curated4
Sequence conflicti629Y → C in AAD43327 (PubMed:10436028).Curated1
Sequence conflicti638R → T in AAD43327 (PubMed:10436028).Curated1
Sequence conflicti642Q → H in AAD43327 (PubMed:10436028).Curated1
Sequence conflicti645F → L in AAD43327 (PubMed:10436028).Curated1
Sequence conflicti672 – 673RA → KP in AAD43327 (PubMed:10436028).Curated2
Sequence conflicti687D → G in AAD43327 (PubMed:10436028).Curated1
Sequence conflicti694A → T in AAD43327 (PubMed:10436028).Curated1
Sequence conflicti705M → V in AAD43327 (PubMed:10436028).Curated1
Sequence conflicti713K → E in AAD43327 (PubMed:10436028).Curated1
Sequence conflicti718 – 720SLH → ALR in AAD43327 (PubMed:10436028).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF155157 mRNA. Translation: AAD43327.1.
AC124698 Genomic DNA. No translation available.
CU210953 Genomic DNA. Translation: CAQ12171.1.
CCDSiCCDS17696.1.
RefSeqiNP_001157024.1. NM_001163552.1.
NP_080469.2. NM_026193.2.
UniGeneiMm.197568.

Genome annotation databases

EnsembliENSMUST00000047285; ENSMUSP00000044262; ENSMUSG00000032952.
ENSMUST00000076599; ENSMUSP00000075904; ENSMUSG00000032952.
GeneIDi67489.
KEGGimmu:67489.
UCSCiuc008qtq.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiAP4B1_MOUSE
AccessioniPrimary (citable) accession number: Q9WV76
Secondary accession number(s): B0V3P2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: October 3, 2012
Last modified: February 28, 2018
This is version 119 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome