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Protein

Ankyrin repeat and SOCS box protein 4

Gene

Asb4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes differentiation and maturation of the vascular lineage by an oxygen-dependent mechanism.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei256 – 2561Essential for interaction with HIF1AN

GO - Molecular functioni

  • enzyme binding Source: UniProtKB
  • ubiquitin protein ligase activity Source: MGI
  • ubiquitin protein ligase binding Source: UniProtKB
  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  • intracellular signal transduction Source: InterPro
  • positive regulation of vasculogenesis Source: UniProtKB
  • protein autoubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ankyrin repeat and SOCS box protein 4
Short name:
ASB-4
Gene namesi
Name:Asb4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1929751. Asb4.

Subcellular locationi

GO - Cellular componenti

  • Cul2-RING ubiquitin ligase complex Source: UniProtKB
  • Cul5-RING ubiquitin ligase complex Source: UniProtKB
  • cytoplasm Source: GO_Central
  • nucleus Source: GO_Central
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi238 – 2381L → D: Abolishes interaction with HIF1AN. 1 Publication
Mutagenesisi239 – 2391L → D: Abolishes interaction with HIF1AN. 1 Publication
Mutagenesisi244 – 2463EVN → ATA: Abolishes interaction with HIF1AN. Fails to induce expansion of the hematovascular lineage in embryonic stem cells. 1 Publication
Mutagenesisi244 – 2441E → A: Impairs interaction with HIF1AN. 1 Publication
Mutagenesisi245 – 2451V → T: Impairs interaction with HIF1AN. 1 Publication
Mutagenesisi246 – 2461N → A: Abolishes interaction with HIF1AN. 1 Publication
Mutagenesisi256 – 2561L → D: Abolishes interaction with HIF1AN. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 426426Ankyrin repeat and SOCS box protein 4PRO_0000066929Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei246 – 2461(3S)-3-hydroxyasparagine; by HIF1AN; partial1 Publication

Post-translational modificationi

Hydroxylation at Asn-246 by HIF1AN may provide an oxygen-dependent regulation mechanism for the function of ASB4 in promoting vascular differentiation.1 Publication

Keywords - PTMi

Hydroxylation

Proteomic databases

MaxQBiQ9WV71.
PaxDbiQ9WV71.
PRIDEiQ9WV71.

PTM databases

iPTMnetiQ9WV71.
PhosphoSiteiQ9WV71.

Expressioni

Tissue specificityi

Highest expression detected in testis, with some expression detected in ovary and heart. Not detected in lung, kidney, liver, spleen and bone marrow.2 Publications

Developmental stagei

Differentially expressed in the vascular lineage during embryonic stem (ES) cell differentiation, with expression increasing when the cells differentiate. At E7.5, no remarkable expression is detected. At E9.5, expressed in intersomitic vessels, dorsal aorta, forelimb buds, allantois/umbilical vessels, vitelline vessels, septum transversum, proepicardium, capillary plexi of the head and branchial arches, endocardium, yolk sac vasculature and placenta. At E10.5, expressed in forelimb and hind limb buds, intersomitic vessels, peripheral liver cells and umbilical vessels, with expression in the capillary plexi of the head and branchial arches no longer detected. At 11.5, high expression levels are limited to forelimbs and hind limbs and the most caudal intersomitic vessels.1 Publication

Gene expression databases

BgeeiENSMUSG00000042607.
CleanExiMM_ASB4.
ExpressionAtlasiQ9WV71. baseline and differential.
GenevisibleiQ9WV71. MM.

Interactioni

Subunit structurei

Interacts with HIF1AN. Component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex formed of CUL2 or CUL5, Elongin BC (TCEB1 and TCEB2), RBX1 and ASB4.1 Publication

GO - Molecular functioni

  • enzyme binding Source: UniProtKB
  • ubiquitin protein ligase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi211144. 3 interactions.
STRINGi10090.ENSMUSP00000040331.

Structurei

3D structure databases

ProteinModelPortaliQ9WV71.
SMRiQ9WV71. Positions 75-317.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati74 – 10330ANK 1Add
BLAST
Repeati106 – 13530ANK 2Add
BLAST
Repeati139 – 16830ANK 3Add
BLAST
Repeati174 – 20330ANK 4Add
BLAST
Repeati207 – 24741ANK 5Add
BLAST
Repeati251 – 28030ANK 6Add
BLAST
Domaini371 – 42353SOCS boxPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni238 – 2392Essential for interaction with HIF1AN
Regioni244 – 2463Essential for interaction with HIF1AN

Domaini

The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin-protein ligase complexes.By similarity

Sequence similaritiesi

Belongs to the ankyrin SOCS box (ASB) family.Curated
Contains 6 ANK repeats.PROSITE-ProRule annotation
Contains 1 SOCS box domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00390000011960.
HOGENOMiHOG000232066.
HOVERGENiHBG024357.
InParanoidiQ9WV71.
KOiK10326.
OMAiHHLICRM.
OrthoDBiEOG091G064L.
PhylomeDBiQ9WV71.
TreeFamiTF331836.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001496. SOCS_box.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF07525. SOCS_box. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 6 hits.
SM00969. SOCS_box. 1 hit.
[Graphical view]
SUPFAMiSSF158235. SSF158235. 1 hit.
SSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 5 hits.
PS50225. SOCS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WV71-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGITAPISK AGAAKLVKKD FLEALKTNDF GKLKAILIER QIDVDTVFEV
60 70 80 90 100
EDENMILASY KQGYWLPSYK LKSSWATGLH LSVLLGHVEC LMVLLDHNAT
110 120 130 140 150
INCRPNGKTP LHVACEIANL ECVKILCDRG AKLNCYSLSG HTALHFCTTP
160 170 180 190 200
SSILCAKQLV LRGANVNMKT NNQDEETPLH TAAHFGLSEL VAFYVENGAI
210 220 230 240 250
VDSMNAHMET PLAIATYWAL RFKEQEYSRE HHLICRTLLD NNAEVNARDD
260 270 280 290 300
DFKSPLHKAA WNCDHVLMHM MLEAGAEANL MDINGCAAIQ YVLKVTSVRP
310 320 330 340 350
AAQPEICYQL LLNHGAARIY PPQFHKVIQA CHSCPKAIEV VVNAYEHIRW
360 370 380 390 400
NIKWRRAIPD DDLEKHWDFY HSLFKVCCNT PRTLMHLSRC AIRKALHNRC
410 420
HKAIPMLSLP LPLKKYLLLE PEGIIY
Length:426
Mass (Da):48,098
Last modified:November 1, 1999 - v1
Checksum:iD8F52119CDF25648
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF155355 mRNA. Translation: AAD38811.1.
AK018373 mRNA. Translation: BAB31183.1.
AK133400 mRNA. Translation: BAE21634.1.
AK133894 mRNA. Translation: BAE21916.1.
BC054745 mRNA. Translation: AAH54745.1.
BC046819 mRNA. Translation: AAH46819.1.
BC056440 mRNA. Translation: AAH56440.1.
CCDSiCCDS19901.1.
RefSeqiNP_075535.1. NM_023048.5.
UniGeneiMm.51340.

Genome annotation databases

EnsembliENSMUST00000043294; ENSMUSP00000040331; ENSMUSG00000042607.
GeneIDi65255.
KEGGimmu:65255.
UCSCiuc009awi.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF155355 mRNA. Translation: AAD38811.1.
AK018373 mRNA. Translation: BAB31183.1.
AK133400 mRNA. Translation: BAE21634.1.
AK133894 mRNA. Translation: BAE21916.1.
BC054745 mRNA. Translation: AAH54745.1.
BC046819 mRNA. Translation: AAH46819.1.
BC056440 mRNA. Translation: AAH56440.1.
CCDSiCCDS19901.1.
RefSeqiNP_075535.1. NM_023048.5.
UniGeneiMm.51340.

3D structure databases

ProteinModelPortaliQ9WV71.
SMRiQ9WV71. Positions 75-317.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211144. 3 interactions.
STRINGi10090.ENSMUSP00000040331.

PTM databases

iPTMnetiQ9WV71.
PhosphoSiteiQ9WV71.

Proteomic databases

MaxQBiQ9WV71.
PaxDbiQ9WV71.
PRIDEiQ9WV71.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043294; ENSMUSP00000040331; ENSMUSG00000042607.
GeneIDi65255.
KEGGimmu:65255.
UCSCiuc009awi.1. mouse.

Organism-specific databases

CTDi51666.
MGIiMGI:1929751. Asb4.

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00390000011960.
HOGENOMiHOG000232066.
HOVERGENiHBG024357.
InParanoidiQ9WV71.
KOiK10326.
OMAiHHLICRM.
OrthoDBiEOG091G064L.
PhylomeDBiQ9WV71.
TreeFamiTF331836.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ9WV71.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042607.
CleanExiMM_ASB4.
ExpressionAtlasiQ9WV71. baseline and differential.
GenevisibleiQ9WV71. MM.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001496. SOCS_box.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF07525. SOCS_box. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 6 hits.
SM00969. SOCS_box. 1 hit.
[Graphical view]
SUPFAMiSSF158235. SSF158235. 1 hit.
SSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 5 hits.
PS50225. SOCS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASB4_MOUSE
AccessioniPrimary (citable) accession number: Q9WV71
Secondary accession number(s): Q3UZE1, Q3V070
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: November 1, 1999
Last modified: September 7, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.