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Protein

Peroxisomal 2,4-dienoyl-CoA reductase

Gene

Decr2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Auxiliary enzyme of beta-oxidation. Participates in the degradation of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions in peroxisome. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA. Has activity towards short and medium chain 2,4-dienoyl-CoAs, but also towards 2,4,7,10,13,16,19-docosaheptaenoyl-CoA, suggesting that it does not constitute a rate limiting step in the peroxisomal degradation of docosahexaenoic acid.

Catalytic activityi

Trans-2,3-didehydroacyl-CoA + NADP+ = trans,trans-2,3,4,5-tetradehydroacyl-CoA + NADPH.1 Publication

Kineticsi

  1. KM=108 µM for 2,4-hexadienoyl-CoA1 Publication
  2. KM=6 µM for 2,4-decadienoyl-CoA1 Publication
  3. KM=155 µM for 2,4,7,10,13,16,19-docosaheptaenoyl-CoA1 Publication
  1. Vmax=8 nmol/min/mg enzyme with 2,4-hexadienoyl-CoA as substrate1 Publication
  2. Vmax=20 nmol/min/mg enzyme with 2,4-decadienoyl-CoA as substrate1 Publication
  3. Vmax=5 nmol/min/mg enzyme with 2,4,7,10,13,16,19-docosaheptaenoyl-CoA as substrate1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei60 – 601SubstrateBy similarity
Binding sitei86 – 861NADPBy similarity
Binding sitei88 – 881SubstrateBy similarity
Binding sitei118 – 1181SubstrateBy similarity
Binding sitei182 – 1821NADPBy similarity
Binding sitei219 – 2191SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi35 – 406NADPBy similarity
Nucleotide bindingi60 – 645NADPBy similarity
Nucleotide bindingi208 – 2147NADPBy similarity

GO - Molecular functioni

GO - Biological processi

  • unsaturated fatty acid biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiR-MMU-535734. Fatty acid, triacylglycerol, and ketone body metabolism.
SABIO-RKQ9WV68.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal 2,4-dienoyl-CoA reductase (EC:1.3.1.34)
Alternative name(s):
2,4-dienoyl-CoA reductase 2
Gene namesi
Name:Decr2
Synonyms:Pdcr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1347059. Decr2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 292291Peroxisomal 2,4-dienoyl-CoA reductasePRO_0000054560Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei64 – 641N6-acetyllysineCombined sources
Modified residuei151 – 1511N6-acetyllysineBy similarity
Modified residuei291 – 2911N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9WV68.
MaxQBiQ9WV68.
PaxDbiQ9WV68.
PRIDEiQ9WV68.

PTM databases

iPTMnetiQ9WV68.
PhosphoSiteiQ9WV68.
SwissPalmiQ9WV68.

Expressioni

Gene expression databases

BgeeiQ9WV68.
CleanExiMM_DECR2.
ExpressionAtlasiQ9WV68. baseline and differential.
GenevisibleiQ9WV68. MM.

Interactioni

Subunit structurei

Monomer, dimer and oligomer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9WV68. 2 interactions.
MINTiMINT-1870221.
STRINGi10090.ENSMUSP00000045621.

Structurei

3D structure databases

ProteinModelPortaliQ9WV68.
SMRiQ9WV68. Positions 3-278.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni126 – 1283Substrate bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi290 – 2923Microbody targeting signalBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0725. Eukaryota.
COG1028. LUCA.
GeneTreeiENSGT00760000118868.
HOVERGENiHBG100327.
InParanoidiQ9WV68.
KOiK13237.
OMAiHTVSIEY.
OrthoDBiEOG789CBR.
PhylomeDBiQ9WV68.
TreeFamiTF315256.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9WV68-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQPPPDVEG DDCLPEYHHL FCPDLLQDKV AFITGGGSGI GFRIAEIFMR
60 70 80 90 100
HGCHTVIVGR SLQKVTTAAK KLVAATGKRC LPLSMDVRVP PEVMTAVDQA
110 120 130 140 150
LQEFGKINIL INCAAGNFLC PASALSFNAF KTVVDIDTIG TFNVSSVLYK
160 170 180 190 200
KFFRDHGGVI VNITATLSMR GQVLQLHAGA AKAAVDAMTR HLAVEWGPQN
210 220 230 240 250
IRVNSLAPGA ISGTEGLRRL RGSNASSKLK HFSNPIPRLG TKTEIAHSVL
260 270 280 290
YLASPLASYV SGIVLVVDGG SWMTFPNGIK QLLEFESFSA KL
Length:292
Mass (Da):31,300
Last modified:November 1, 1999 - v1
Checksum:iE25ED366830CC6AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF155575 mRNA. Translation: AAD38196.1.
BC021865 mRNA. Translation: AAH21865.1.
CCDSiCCDS28543.1.
RefSeqiNP_036063.1. NM_011933.2.
UniGeneiMm.292869.

Genome annotation databases

EnsembliENSMUST00000040907; ENSMUSP00000045621; ENSMUSG00000036775.
GeneIDi26378.
KEGGimmu:26378.
UCSCiuc008bdg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF155575 mRNA. Translation: AAD38196.1.
BC021865 mRNA. Translation: AAH21865.1.
CCDSiCCDS28543.1.
RefSeqiNP_036063.1. NM_011933.2.
UniGeneiMm.292869.

3D structure databases

ProteinModelPortaliQ9WV68.
SMRiQ9WV68. Positions 3-278.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9WV68. 2 interactions.
MINTiMINT-1870221.
STRINGi10090.ENSMUSP00000045621.

PTM databases

iPTMnetiQ9WV68.
PhosphoSiteiQ9WV68.
SwissPalmiQ9WV68.

Proteomic databases

EPDiQ9WV68.
MaxQBiQ9WV68.
PaxDbiQ9WV68.
PRIDEiQ9WV68.

Protocols and materials databases

DNASUi26378.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040907; ENSMUSP00000045621; ENSMUSG00000036775.
GeneIDi26378.
KEGGimmu:26378.
UCSCiuc008bdg.1. mouse.

Organism-specific databases

CTDi26063.
MGIiMGI:1347059. Decr2.

Phylogenomic databases

eggNOGiKOG0725. Eukaryota.
COG1028. LUCA.
GeneTreeiENSGT00760000118868.
HOVERGENiHBG100327.
InParanoidiQ9WV68.
KOiK13237.
OMAiHTVSIEY.
OrthoDBiEOG789CBR.
PhylomeDBiQ9WV68.
TreeFamiTF315256.

Enzyme and pathway databases

ReactomeiR-MMU-535734. Fatty acid, triacylglycerol, and ketone body metabolism.
SABIO-RKQ9WV68.

Miscellaneous databases

NextBioi304285.
PROiQ9WV68.
SOURCEiSearch...

Gene expression databases

BgeeiQ9WV68.
CleanExiMM_DECR2.
ExpressionAtlasiQ9WV68. baseline and differential.
GenevisibleiQ9WV68. MM.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The mouse gene PDCR encodes a peroxisomal delta(2), delta(4)-dienoyl-CoA reductase."
    Geisbrecht B.V., Liang X., Morrell J.C., Schulz H., Gould S.J.
    J. Biol. Chem. 274:25814-25820(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME ACTIVITY, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney and Liver.
  4. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-64, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiDECR2_MOUSE
AccessioniPrimary (citable) accession number: Q9WV68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: November 1, 1999
Last modified: May 11, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.