Glycogen synthase kinase-3 beta - Mus musculus (Mouse)

Contribute

Send feedback

Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q9WV60 (GSK3B_MOUSE)

Last modified December 15, 2009. Version 98. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data |

Customize display

text xml rdf/xml gff fasta

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein names

Recommended name:
    Glycogen synthase kinase-3 beta
      Short name=GSK-3 beta
    EC=2.7.11.26

Gene names

Name:

Gsk3b

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Hide | Top

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

Hide | Top

General annotation (Comments)

Function	Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. May phosphorylate MUC1 and decrease the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin By similarity.
Catalytic activity	ATP + [tau protein] = ADP + [tau protein] phosphate.
Subunit structure	Monomer. Interacts with AXIN1, CABYR, MUC1, NIN and PRUNE By similarity. Interacts with ARRB2.
Post-translational modification	Phosphorylation on Tyr-216 is necessary for the activity By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily. Contains 1 protein kinase domain.

Hide | Top

Ontologies

Keywords
Biological process	Wnt signaling pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	ER overload response Inferred from direct assay. Source: MGI Wnt receptor signaling pathway Inferred from genetic interaction. Source: MGI anti-apoptosis Inferred from mutant phenotype. Source: MGI cell proliferation Traceable author statement. Source: MGI fat cell differentiation Inferred from direct assay. Source: MGI organ morphogenesis Inferred from mutant phenotype. Source: MGI protein amino acid phosphorylation Inferred from direct assay. Source: MGI protein export from nucleus Inferred from direct assay. Source: MGI re-entry into mitotic cell cycle Inferred from direct assay. Source: MGI
Cellular component	cell soma Inferred from direct assay. Source: MGI cytosol Inferred from direct assay. Source: MGI dendritic shaft Inferred from direct assay. Source: MGI growth cone Inferred from direct assay. Source: MGI nucleoplasm Inferred from Experiment. Source: Reactome
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW beta-catenin binding Inferred from physical interaction. Source: MGI glycogen synthase kinase 3 activity Inferred from direct assay. Source: MGI tau-protein kinase activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Hide | Top

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Disc1	Q811T9	1	EBI-400793,EBI-2298259
Epm2a	Q9WUA5	2	EBI-400793,EBI-1040928

Hide | Top

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 420

420

Glycogen synthase kinase-3 beta

PRO_0000085981

Regions

Domain

56 – 340

285

Protein kinase

Nucleotide binding

62 – 70

ATP By similarity

Sites

Active site

181

Proton acceptor By similarity

Binding site

ATP By similarity

Amino acid modifications

Modified residue

Phosphothreonine Ref.11

Modified residue

Phosphoserine; by PKB/AKT1 By similarity

Modified residue

215

Phosphoserine Ref.10 Ref.12

Modified residue

216

Phosphotyrosine Ref.11 Ref.10 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9

Modified residue

219

Phosphoserine Ref.11 Ref.7 Ref.9

Modified residue

275

Phosphothreonine By similarity

Modified residue

277

Phosphothreonine By similarity

Modified residue

389

Phosphoserine Ref.10 Ref.3

Modified residue

395

Phosphothreonine Ref.10

Hide | Top

Sequences

Sequence

Length

Mass (Da)

Tools

Q9WV60-1 [UniParc].

Last modified May 1, 2000. Version 2.
Checksum: 200C3FD1B38B4883
Show »

FASTA

420

46,710

        10         20         30         40         50         60 
MSGRPRTTSF AESCKPVQQP SAFGSMKVSR DKDGSKVTTV VATPGQGPDR PQEVSYTDTK 

        70         80         90        100        110        120 
VIGNGSFGVV YQAKLCDSGE LVAIKKVLQD KRFKNRELQI MRKLDHCNIV RLRYFFYSSG 

       130        140        150        160        170        180 
EKKDEVYLNL VLDYVPETVY RVARHYSRAK QTLPVIYVKL YMYQLFRSLA YIHSFGICHR 

       190        200        210        220        230        240 
DIKPQNLLLD PDTAVLKLCD FGSAKQLVRG EPNVSYICSR YYRAPELIFG ATDYTSSIDV 

       250        260        270        280        290        300 
WSAGCVLAEL LLGQPIFPGD SGVDQLVEII KVLGTPTREQ IREMNPNYTE FKFPQIKAHP 

       310        320        330        340        350        360 
WTKVFRPRTP PEAIALCSRL LEYTPTARLT PLEACAHSFF DELRDPNVKL PNGRDTPALF 

       370        380        390        400        410        420 
NFTTQELSSN PPLATILIPP HARIQAAASP PANATAASDT NAGDRGQTNN AASASASNST

« Hide

Hide | Top

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Testicular expression and hormonal control of glycogen synthase kinase 3, a homologue of yeast RIM11." Salameh W.A., Guo T.B., Chan K.C., Mitchell A.P. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II and FVB/N. Tissue: Mammary gland.
[3]	"Phosphoproteomic analysis of the developing mouse brain." Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P. Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed: 15345747] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-389, MASS SPECTROMETRY. Tissue: Brain.
[4]	"An Akt/beta-arrestin 2/PP2A signaling complex mediates dopaminergic neurotransmission and behavior." Beaulieu J.-M., Sotnikova T.D., Marion S., Lefkowitz R.J., Gainetdinov R.R., Caron M.G. Cell 122:261-273(2005) [PubMed: 16051150] [Abstract] Cited for: INTERACTION WITH ARRB2.
[5]	"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2005) [PubMed: 15592455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-216, MASS SPECTROMETRY.
[6]	"Comprehensive identification of phosphorylation sites in postsynaptic density preparations." Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L. Mol. Cell. Proteomics 5:914-922(2006) [PubMed: 16452087] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-216, MASS SPECTROMETRY. Tissue: Brain.
[7]	"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling." Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R. J. Immunol. 179:5864-5876(2007) [PubMed: 17947660] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-216 AND SER-219, MASS SPECTROMETRY. Tissue: Mast cell.
[8]	"Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networks." Wolf-Yadlin A., Hautaniemi S., Lauffenburger D.A., White F.M. Proc. Natl. Acad. Sci. U.S.A. 104:5860-5865(2007) [PubMed: 17389395] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-216, MASS SPECTROMETRY.
[9]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed: 18034455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-216 AND SER-219, MASS SPECTROMETRY. Tissue: Brain.
[10]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed: 17114649] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-215; TYR-216; SER-389 AND THR-395, MASS SPECTROMETRY. Tissue: Brain cortex.
[11]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-7; TYR-216 AND SER-219, MASS SPECTROMETRY. Tissue: Liver.
[12]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed: 19131326] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-215, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases
	AF156099 mRNA. Translation: AAD39258.2. BC006936 mRNA. Translation: AAH06936.1. BC060743 mRNA. Translation: AAH60743.1.
IPI	IPI00125319.
RefSeq	NP_062801.1.
UniGene	Mm.394930
3D structure databases
SMR	Q9WV60. Positions 23-386.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9WV60. 4 interactions.
STRING	Q9WV60.
PTM databases
PhosphoSite	Q9WV60.
Proteomic databases
PRIDE	Q9WV60.
Genome annotation databases
Ensembl	ENSMUST00000023507; ENSMUSP00000023507; ENSMUSG00000022812; Mus musculus. [Genome view]
GeneID	56637.
KEGG	mmu:56637.
UCSC	uc007zen.1. mouse.
Organism-specific databases
CTD	56637.
MGI	MGI:1861437. Gsk3b.
Phylogenomic databases
HOVERGEN	Q9WV60.
OrthoDB	EOG9R52NK.
Enzyme and pathway databases
BRENDA	2.7.11.26. 244.
Gene expression databases
ArrayExpress	Q9WV60.
Bgee	Q9WV60.
CleanEx	MM_GSK3B.
Genevestigator	Q9WV60.
GermOnline	ENSMUSG00000022812. Mus musculus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	313081.
PMAP-CutDB	Q9WV60.
SOURCE	Search...

Hide | Top

Entry information

Entry name

GSK3B_MOUSE

Accession

Primary (citable) accession number: Q9WV60

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	December 15, 2009
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Hide | Top

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents