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Protein

Growth/differentiation factor 2

Gene

Gdf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Potent circulating inhibitor of angiogenesis. Could be involved in bone formation. Signals through the type I activin receptor ACVRL1 but not other Alks. Signaling through SMAD1 in endothelial cells requires TGF-beta coreceptor endoglin/ENG.1 Publication

GO - Molecular functioni

GO - Biological processi

  • activin receptor signaling pathway Source: MGI
  • angiogenesis Source: UniProtKB
  • blood vessel morphogenesis Source: MGI
  • BMP signaling pathway Source: MGI
  • cell development Source: GO_Central
  • cellular iron ion homeostasis Source: DFLAT
  • cellular response to BMP stimulus Source: MGI
  • glucose metabolic process Source: DFLAT
  • growth Source: InterPro
  • negative regulation of cell growth Source: MGI
  • negative regulation of DNA biosynthetic process Source: MGI
  • negative regulation of endothelial cell migration Source: MGI
  • negative regulation of endothelial cell proliferation Source: MGI
  • neuron differentiation Source: DFLAT
  • osteoblast differentiation Source: MGI
  • pathway-restricted SMAD protein phosphorylation Source: MGI
  • patterning of blood vessels Source: DFLAT
  • positive regulation of angiogenesis Source: DFLAT
  • positive regulation of BMP signaling pathway Source: DFLAT
  • positive regulation of cartilage development Source: UniProtKB
  • positive regulation of endothelial cell differentiation Source: DFLAT
  • positive regulation of endothelial cell proliferation Source: DFLAT
  • positive regulation of gene expression Source: MGI
  • positive regulation of interleukin-8 production Source: MGI
  • positive regulation of osteoblast differentiation Source: MGI
  • positive regulation of pathway-restricted SMAD protein phosphorylation Source: MGI
  • positive regulation of transcription, DNA-templated Source: DFLAT
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of apoptotic process Source: GO_Central
  • regulation of MAPK cascade Source: GO_Central
  • SMAD protein signal transduction Source: GO_Central
  • vasculogenesis Source: DFLAT
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Growth factor

Keywords - Biological processi

Angiogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Growth/differentiation factor 2
Short name:
GDF-2
Alternative name(s):
Bone morphogenetic protein 9
Short name:
BMP-9
Gene namesi
Name:Gdf2
Synonyms:Bmp9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1321394. Gdf2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
PropeptideiPRO_000003390423 – 318By similarityAdd BLAST296
ChainiPRO_0000033905319 – 428Growth/differentiation factor 2Add BLAST110

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi70N-linked (GlcNAc...)Sequence analysis1
Glycosylationi135N-linked (GlcNAc...)Sequence analysis1
Glycosylationi262N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi326 ↔ 392By similarity
Disulfide bondi355 ↔ 425By similarity
Disulfide bondi359 ↔ 427By similarity
Disulfide bondi391InterchainBy similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9WV56.
PRIDEiQ9WV56.

PTM databases

iPTMnetiQ9WV56.
PhosphoSitePlusiQ9WV56.

Expressioni

Gene expression databases

BgeeiENSMUSG00000072625.
CleanExiMM_GDF2.
GenevisibleiQ9WV56. MM.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Interacts with ENG (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000098286.

Structurei

Secondary structure

1428
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi86 – 96Combined sources11
Beta strandi106 – 112Combined sources7
Beta strandi115 – 119Combined sources5
Beta strandi123 – 125Combined sources3
Beta strandi127 – 133Combined sources7
Beta strandi142 – 155Combined sources14
Helixi161 – 163Combined sources3
Beta strandi167 – 176Combined sources10
Beta strandi185 – 196Combined sources12
Beta strandi198 – 205Combined sources8
Helixi207 – 214Combined sources8
Turni217 – 219Combined sources3
Beta strandi223 – 231Combined sources9
Beta strandi233 – 235Combined sources3
Beta strandi239 – 241Combined sources3
Beta strandi252 – 259Combined sources8
Turni260 – 263Combined sources4
Helixi264 – 277Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4YCGX-ray3.30A/B23-318[»]
4YCIX-ray3.25A/B23-318[»]
ProteinModelPortaliQ9WV56.
SMRiQ9WV56.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TGF-beta family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3900. Eukaryota.
ENOG410XT8Z. LUCA.
GeneTreeiENSGT00760000118883.
HOGENOMiHOG000249477.
HOVERGENiHBG106648.
InParanoidiQ9WV56.
KOiK05503.
OMAiENMKVDF.
OrthoDBiEOG091G075U.
TreeFamiTF316134.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR002405. Inhibin_asu.
IPR001839. TGF-b_C.
IPR001111. TGF-b_N.
IPR015615. TGF-beta-rel.
IPR017948. TGFb_CS.
[Graphical view]
PANTHERiPTHR11848. PTHR11848. 2 hits.
PfamiPF00019. TGF_beta. 1 hit.
PF00688. TGFb_propeptide. 1 hit.
[Graphical view]
PRINTSiPR00669. INHIBINA.
SMARTiSM00204. TGFB. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00250. TGF_BETA_1. 1 hit.
PS51362. TGF_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9WV56-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPGAFRVAL LPLFLLVCVT QQKPLQNWEQ ASPGENAHSS LGLSGAGEEG
60 70 80 90 100
VFDLQMFLEN MKVDFLRSLN LSGIPSQDKT RAEPPQYMID LYNRYTTDKS
110 120 130 140 150
STPASNIVRS FSVEDAISTA ATEDFPFQKH ILIFNISIPR HEQITRAELR
160 170 180 190 200
LYVSCQNDVD STHGLEGSMV VYDVLEDSET WDQATGTKTF LVSQDIRDEG
210 220 230 240 250
WETLEVSSAV KRWVRADSTT NKNKLEVTVQ SHRESCDTLD ISVPPGSKNL
260 270 280 290 300
PFFVVFSNDR SNGTKETRLE LKEMIGHEQE TMLVKTAKNA YQVAGESQEE
310 320 330 340 350
EGLDGYTAVG PLLARRKRST GASSHCQKTS LRVNFEDIGW DSWIIAPKEY
360 370 380 390 400
DAYECKGGCF FPLADDVTPT KHAIVQTLVH LKFPTKVGKA CCVPTKLSPI
410 420
SILYKDDMGV PTLKYHYEGM SVAECGCR
Length:428
Mass (Da):47,703
Last modified:July 27, 2011 - v2
Checksum:i82E4475FF8B27F36
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti293V → G in AAD40308 (Ref. 1) Curated1
Sequence conflicti382K → E in AAD56961 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF156890 mRNA. Translation: AAD40308.1.
AF188286 mRNA. Translation: AAD56961.1.
AK147029 mRNA. Translation: BAE27621.1.
CH466573 Genomic DNA. Translation: EDL24862.1.
BC103625 mRNA. Translation: AAI03626.1.
BC103679 mRNA. Translation: AAI03680.1.
BC103680 mRNA. Translation: AAI03681.1.
BC103681 mRNA. Translation: AAI03682.1.
CCDSiCCDS26928.1.
RefSeqiNP_062379.3. NM_019506.4.
UniGeneiMm.422844.

Genome annotation databases

EnsembliENSMUST00000100720; ENSMUSP00000098286; ENSMUSG00000072625.
GeneIDi12165.
KEGGimmu:12165.
UCSCiuc007tac.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF156890 mRNA. Translation: AAD40308.1.
AF188286 mRNA. Translation: AAD56961.1.
AK147029 mRNA. Translation: BAE27621.1.
CH466573 Genomic DNA. Translation: EDL24862.1.
BC103625 mRNA. Translation: AAI03626.1.
BC103679 mRNA. Translation: AAI03680.1.
BC103680 mRNA. Translation: AAI03681.1.
BC103681 mRNA. Translation: AAI03682.1.
CCDSiCCDS26928.1.
RefSeqiNP_062379.3. NM_019506.4.
UniGeneiMm.422844.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4YCGX-ray3.30A/B23-318[»]
4YCIX-ray3.25A/B23-318[»]
ProteinModelPortaliQ9WV56.
SMRiQ9WV56.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000098286.

PTM databases

iPTMnetiQ9WV56.
PhosphoSitePlusiQ9WV56.

Proteomic databases

PaxDbiQ9WV56.
PRIDEiQ9WV56.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000100720; ENSMUSP00000098286; ENSMUSG00000072625.
GeneIDi12165.
KEGGimmu:12165.
UCSCiuc007tac.1. mouse.

Organism-specific databases

CTDi2658.
MGIiMGI:1321394. Gdf2.

Phylogenomic databases

eggNOGiKOG3900. Eukaryota.
ENOG410XT8Z. LUCA.
GeneTreeiENSGT00760000118883.
HOGENOMiHOG000249477.
HOVERGENiHBG106648.
InParanoidiQ9WV56.
KOiK05503.
OMAiENMKVDF.
OrthoDBiEOG091G075U.
TreeFamiTF316134.

Miscellaneous databases

PROiQ9WV56.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000072625.
CleanExiMM_GDF2.
GenevisibleiQ9WV56. MM.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR002405. Inhibin_asu.
IPR001839. TGF-b_C.
IPR001111. TGF-b_N.
IPR015615. TGF-beta-rel.
IPR017948. TGFb_CS.
[Graphical view]
PANTHERiPTHR11848. PTHR11848. 2 hits.
PfamiPF00019. TGF_beta. 1 hit.
PF00688. TGFb_propeptide. 1 hit.
[Graphical view]
PRINTSiPR00669. INHIBINA.
SMARTiSM00204. TGFB. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00250. TGF_BETA_1. 1 hit.
PS51362. TGF_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGDF2_MOUSE
AccessioniPrimary (citable) accession number: Q9WV56
Secondary accession number(s): Q3ZAS6, Q9QZE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.