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Q9WV56 (GDF2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Growth/differentiation factor 2

Short name=GDF-2
Alternative name(s):
Bone morphogenetic protein 9
Short name=BMP-9
Gene names
Name:Gdf2
Synonyms:Bmp9
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length428 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Potent circulating inhibitor of angiogenesis. Could be involved in bone formation. Signals through the type I activin receptor ACVRL1 but not other Alks. Signaling through SMAD1 in endothelial cells requires TGF-beta coreceptor endoglin/ENG. Ref.6

Subunit structure

Homodimer; disulfide-linked. Interacts with ENG By similarity.

Subcellular location

Secreted.

Sequence similarities

Belongs to the TGF-beta family.

Ontologies

Keywords
   Biological processAngiogenesis
   Cellular componentSecreted
   DomainSignal
   Molecular functionCytokine
Growth factor
   PTMCleavage on pair of basic residues
Disulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processBMP signaling pathway

Inferred from electronic annotation. Source: Ensembl

activin receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

cellular iron ion homeostasis

Traceable author statement PubMed 20406889. Source: DFLAT

glucose metabolic process

Traceable author statement PubMed 20406889. Source: DFLAT

growth

Inferred from electronic annotation. Source: InterPro

negative regulation of DNA biosynthetic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of cell growth

Inferred from electronic annotation. Source: Ensembl

negative regulation of endothelial cell migration

Inferred from electronic annotation. Source: Ensembl

negative regulation of endothelial cell proliferation

Inferred from electronic annotation. Source: Ensembl

neuron differentiation

Traceable author statement PubMed 20406889. Source: DFLAT

osteoblast differentiation

Inferred from genetic interaction PubMed 18986983. Source: MGI

pathway-restricted SMAD protein phosphorylation

Inferred from electronic annotation. Source: Ensembl

patterning of blood vessels

Inferred from direct assay PubMed 20406889. Source: DFLAT

positive regulation of BMP signaling pathway

Inferred from direct assay PubMed 20406889. Source: DFLAT

positive regulation of angiogenesis

Inferred from direct assay PubMed 20406889. Source: DFLAT

positive regulation of endothelial cell differentiation

Inferred from direct assay PubMed 20406889. Source: DFLAT

positive regulation of endothelial cell proliferation

Inferred from direct assay PubMed 20406889. Source: DFLAT

positive regulation of gene expression

Inferred from direct assay PubMed 22783020. Source: MGI

positive regulation of interleukin-8 production

Inferred from electronic annotation. Source: Ensembl

positive regulation of osteoblast differentiation

Inferred from genetic interaction PubMed 15161906. Source: MGI

positive regulation of pathway-restricted SMAD protein phosphorylation

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from genetic interaction PubMed 18986983. Source: MGI

positive regulation of transcription, DNA-templated

Inferred from mutant phenotype PubMed 20406889. Source: DFLAT

vasculogenesis

Inferred from direct assay PubMed 20406889. Source: DFLAT

   Cellular_componentextracellular space

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Propeptide23 – 318296 By similarity
PRO_0000033904
Chain319 – 428110Growth/differentiation factor 2
PRO_0000033905

Amino acid modifications

Glycosylation701N-linked (GlcNAc...) Potential
Glycosylation1351N-linked (GlcNAc...) Potential
Glycosylation2621N-linked (GlcNAc...) Potential
Disulfide bond326 ↔ 392 By similarity
Disulfide bond355 ↔ 425 By similarity
Disulfide bond359 ↔ 427 By similarity
Disulfide bond391Interchain By similarity

Experimental info

Sequence conflict2931V → G in AAD40308. Ref.1
Sequence conflict3821K → E in AAD56961. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9WV56 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 82E4475FF8B27F36

FASTA42847,703
        10         20         30         40         50         60 
MSPGAFRVAL LPLFLLVCVT QQKPLQNWEQ ASPGENAHSS LGLSGAGEEG VFDLQMFLEN 

        70         80         90        100        110        120 
MKVDFLRSLN LSGIPSQDKT RAEPPQYMID LYNRYTTDKS STPASNIVRS FSVEDAISTA 

       130        140        150        160        170        180 
ATEDFPFQKH ILIFNISIPR HEQITRAELR LYVSCQNDVD STHGLEGSMV VYDVLEDSET 

       190        200        210        220        230        240 
WDQATGTKTF LVSQDIRDEG WETLEVSSAV KRWVRADSTT NKNKLEVTVQ SHRESCDTLD 

       250        260        270        280        290        300 
ISVPPGSKNL PFFVVFSNDR SNGTKETRLE LKEMIGHEQE TMLVKTAKNA YQVAGESQEE 

       310        320        330        340        350        360 
EGLDGYTAVG PLLARRKRST GASSHCQKTS LRVNFEDIGW DSWIIAPKEY DAYECKGGCF 

       370        380        390        400        410        420 
FPLADDVTPT KHAIVQTLVH LKFPTKVGKA CCVPTKLSPI SILYKDDMGV PTLKYHYEGM 


SVAECGCR 

« Hide

References

« Hide 'large scale' references
[1]"Growth/differentiation factor-2, a new TGF-beta family member with bone promoting activities."
Zimmers T.A., Koniaris L.G., Sitzmann J.V., Lee S.-J.
Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: CD-1.
Tissue: Liver.
[2]Celeste A.J.
Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Liver.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"Endoglin requirement for BMP9 signaling in endothelial cells reveals new mechanism of action for selective anti-endoglin antibodies."
Nolan-Stevaux O., Zhong W., Culp S., Shaffer K., Hoover J., Wickramasinghe D., Ruefli-Brasse A.
PLoS ONE 7:E50920-E50920(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF156890 mRNA. Translation: AAD40308.1.
AF188286 mRNA. Translation: AAD56961.1.
AK147029 mRNA. Translation: BAE27621.1.
CH466573 Genomic DNA. Translation: EDL24862.1.
BC103625 mRNA. Translation: AAI03626.1.
BC103679 mRNA. Translation: AAI03680.1.
BC103680 mRNA. Translation: AAI03681.1.
BC103681 mRNA. Translation: AAI03682.1.
RefSeqNP_062379.3. NM_019506.4.
UniGeneMm.422844.

3D structure databases

ProteinModelPortalQ9WV56.
SMRQ9WV56. Positions 324-428.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ9WV56.

Proteomic databases

PRIDEQ9WV56.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000100720; ENSMUSP00000098286; ENSMUSG00000072625.
GeneID12165.
KEGGmmu:12165.
UCSCuc007tac.1. mouse.

Organism-specific databases

CTD2658.
MGIMGI:1321394. Gdf2.

Phylogenomic databases

eggNOGNOG280573.
GeneTreeENSGT00740000115456.
HOGENOMHOG000249477.
HOVERGENHBG106648.
InParanoidQ3ZAS6.
KOK05503.
OMAENMKVDF.
OrthoDBEOG7ZD1V3.
TreeFamTF316134.

Gene expression databases

BgeeQ9WV56.
CleanExMM_GDF2.
GenevestigatorQ9WV56.

Family and domain databases

InterProIPR002405. Inhibin_asu.
IPR001839. TGF-b_C.
IPR001111. TGF-b_N.
IPR015615. TGF-beta-rel.
IPR017948. TGFb_CS.
[Graphical view]
PANTHERPTHR11848. PTHR11848. 1 hit.
PfamPF00019. TGF_beta. 1 hit.
PF00688. TGFb_propeptide. 1 hit.
[Graphical view]
PRINTSPR00669. INHIBINA.
SMARTSM00204. TGFB. 1 hit.
[Graphical view]
PROSITEPS00250. TGF_BETA_1. 1 hit.
PS51362. TGF_BETA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio280527.
PROQ9WV56.
SOURCESearch...

Entry information

Entry nameGDF2_MOUSE
AccessionPrimary (citable) accession number: Q9WV56
Secondary accession number(s): Q3ZAS6, Q9QZE0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot