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Protein

Vesicle-associated membrane protein-associated protein A

Gene

Vapa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to OSBPL3, which mediates recruitment of VAPA to plasma membrane sites. The ORP3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface. With OSBPL3, may regulate ER morphology. May play a role in vesicle trafficking.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-1660661. Sphingolipid de novo biosynthesis.
R-MMU-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein-associated protein A
Short name:
VAMP-A
Short name:
VAMP-associated protein A
Short name:
VAP-A
Alternative name(s):
33 kDa VAMP-associated protein
Short name:
VAP-33
Gene namesi
Name:Vapa
Synonyms:Vap33
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1353561. Vapa.

Subcellular locationi

  • Endoplasmic reticulum membrane 1 Publication; Single-pass type IV membrane protein By similarity
  • Cell membrane By similarity; Single-pass type IV membrane protein Curated
  • Cell junctiontight junction By similarity
  • Nucleus membrane By similarity

  • Note: Present in the plasma membrane and in intracellular vesicles, together with SNARE proteins. May also associate with the cytoskeleton. Colocalizes with OCLN at the tight junction in polarized epithelial cells.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 228CytoplasmicSequence analysisAdd BLAST227
Transmembranei229 – 249Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Endoplasmic reticulum, Membrane, Nucleus, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002134712 – 249Vesicle-associated membrane protein-associated protein AAdd BLAST248

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei125N6-acetyllysineBy similarity1
Modified residuei166PhosphoserineBy similarity1
Modified residuei170PhosphothreonineBy similarity1
Modified residuei214PhosphoserineBy similarity1
Modified residuei216PhosphoserineBy similarity1
Modified residuei219PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9WV55.
MaxQBiQ9WV55.
PaxDbiQ9WV55.
PeptideAtlasiQ9WV55.
PRIDEiQ9WV55.
TopDownProteomicsiQ9WV55.

PTM databases

iPTMnetiQ9WV55.
PhosphoSitePlusiQ9WV55.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSMUSG00000024091.
GenevisibleiQ9WV55. MM.

Interactioni

Subunit structurei

Homodimer, and heterodimer with VAPB. Interacts with VAMP1, VAMP2, STX1A, BET1, SEC22C and with the C-terminal domain of OCLN. Interacts with OSBPL1A (By similarity). Interacts (via MSP domain) with ZFYVE27 (PubMed:24251978). May retain ZFYVE27 in the endoplasmic reticulum and regulate its function in cell projections formation. Interacts with OSBP. Interacts (via C-terminus) with RSAD2/viperin (via C-terminus). Interacts with OSBPL3 (phosphorylated form). Interacts with KIF5A in a ZFYVE27-dependent manner (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi206035. 4 interactors.
IntActiQ9WV55. 14 interactors.
MINTiMINT-4998482.
STRINGi10090.ENSMUSP00000024897.

Structurei

Secondary structure

1249
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi15 – 26Combined sources12
Beta strandi29 – 31Combined sources3
Beta strandi35 – 40Combined sources6
Beta strandi43 – 45Combined sources3
Beta strandi47 – 54Combined sources8
Beta strandi58 – 67Combined sources10
Beta strandi73 – 80Combined sources8
Beta strandi95 – 101Combined sources7
Helixi109 – 115Combined sources7
Turni118 – 120Combined sources3
Beta strandi122 – 131Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CRINMR-A8-141[»]
ProteinModelPortaliQ9WV55.
SMRiQ9WV55.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WV55.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 131MSPPROSITE-ProRule annotationAdd BLAST118

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili168 – 207Sequence analysisAdd BLAST40

Sequence similaritiesi

Contains 1 MSP domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0439. Eukaryota.
COG5066. LUCA.
GeneTreeiENSGT00390000006947.
HOGENOMiHOG000293182.
HOVERGENiHBG028551.
InParanoidiQ9WV55.
KOiK06096.
OMAiFEMPSEN.
OrthoDBiEOG091G0KJ2.
PhylomeDBiQ9WV55.
TreeFamiTF317024.

Family and domain databases

Gene3Di2.60.40.360. 1 hit.
InterProiIPR000535. MSP_dom.
IPR008962. PapD-like.
IPR016763. VAP.
IPR030229. VAPA.
[Graphical view]
PANTHERiPTHR10809. PTHR10809. 1 hit.
PTHR10809:SF40. PTHR10809:SF40. 1 hit.
PfamiPF00635. Motile_Sperm. 1 hit.
[Graphical view]
PIRSFiPIRSF019693. VAMP-associated. 1 hit.
SUPFAMiSSF49354. SSF49354. 1 hit.
PROSITEiPS50202. MSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9WV55-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASASGAMAK HEQILVLDPP SDLKFKGPFT DVVTTNLKLQ NPSDRKVCFK
60 70 80 90 100
VKTTAPRRYC VRPNSGIIDP GSIVTVSVML QPFDYDPNEK SKHKFMVQTI
110 120 130 140 150
FAPPNISDME AVWKEAKPDE LMDSKLRCVF EMPNENDKLN DMEPSKAVPL
160 170 180 190 200
NASKQDGPLP KPHSVSLNDT ETRKLMEECK RLQGEMMKLS EENRHLRDEG
210 220 230 240
LRLRKVAHSD KPGSTSAVSF RDNVTSPLPS LLVVIAAIFI GFFLGKFIL
Length:249
Mass (Da):27,855
Last modified:January 9, 2007 - v2
Checksum:iFE8F956C1F2C71CA
GO

Sequence cautioni

The sequence AAD45320 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB22868 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti10 – 11KH → ND in AAF23076 (Ref. 4) Curated2
Sequence conflicti154K → R in AAF23076 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF157497 mRNA. Translation: AAD45320.1. Different initiation.
AK003576 mRNA. Translation: BAB22868.1. Different initiation.
AK167381 mRNA. Translation: BAE39474.1.
AK168824 mRNA. Translation: BAE40651.1.
AK159582 mRNA. Translation: BAE35202.1.
BC003866 mRNA. Translation: AAH03866.2.
AF115503 mRNA. Translation: AAF23076.1.
CCDSiCCDS37677.1.
RefSeqiNP_038961.2. NM_013933.3.
UniGeneiMm.266767.
Mm.476204.

Genome annotation databases

EnsembliENSMUST00000024897; ENSMUSP00000024897; ENSMUSG00000024091.
GeneIDi30960.
KEGGimmu:30960.
UCSCiuc008dgd.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF157497 mRNA. Translation: AAD45320.1. Different initiation.
AK003576 mRNA. Translation: BAB22868.1. Different initiation.
AK167381 mRNA. Translation: BAE39474.1.
AK168824 mRNA. Translation: BAE40651.1.
AK159582 mRNA. Translation: BAE35202.1.
BC003866 mRNA. Translation: AAH03866.2.
AF115503 mRNA. Translation: AAF23076.1.
CCDSiCCDS37677.1.
RefSeqiNP_038961.2. NM_013933.3.
UniGeneiMm.266767.
Mm.476204.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CRINMR-A8-141[»]
ProteinModelPortaliQ9WV55.
SMRiQ9WV55.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206035. 4 interactors.
IntActiQ9WV55. 14 interactors.
MINTiMINT-4998482.
STRINGi10090.ENSMUSP00000024897.

PTM databases

iPTMnetiQ9WV55.
PhosphoSitePlusiQ9WV55.

Proteomic databases

EPDiQ9WV55.
MaxQBiQ9WV55.
PaxDbiQ9WV55.
PeptideAtlasiQ9WV55.
PRIDEiQ9WV55.
TopDownProteomicsiQ9WV55.

Protocols and materials databases

DNASUi30960.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000024897; ENSMUSP00000024897; ENSMUSG00000024091.
GeneIDi30960.
KEGGimmu:30960.
UCSCiuc008dgd.2. mouse.

Organism-specific databases

CTDi9218.
MGIiMGI:1353561. Vapa.

Phylogenomic databases

eggNOGiKOG0439. Eukaryota.
COG5066. LUCA.
GeneTreeiENSGT00390000006947.
HOGENOMiHOG000293182.
HOVERGENiHBG028551.
InParanoidiQ9WV55.
KOiK06096.
OMAiFEMPSEN.
OrthoDBiEOG091G0KJ2.
PhylomeDBiQ9WV55.
TreeFamiTF317024.

Enzyme and pathway databases

ReactomeiR-MMU-1660661. Sphingolipid de novo biosynthesis.
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

ChiTaRSiVapa. mouse.
EvolutionaryTraceiQ9WV55.
PROiQ9WV55.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024091.
GenevisibleiQ9WV55. MM.

Family and domain databases

Gene3Di2.60.40.360. 1 hit.
InterProiIPR000535. MSP_dom.
IPR008962. PapD-like.
IPR016763. VAP.
IPR030229. VAPA.
[Graphical view]
PANTHERiPTHR10809. PTHR10809. 1 hit.
PTHR10809:SF40. PTHR10809:SF40. 1 hit.
PfamiPF00635. Motile_Sperm. 1 hit.
[Graphical view]
PIRSFiPIRSF019693. VAMP-associated. 1 hit.
SUPFAMiSSF49354. SSF49354. 1 hit.
PROSITEiPS50202. MSP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVAPA_MOUSE
AccessioniPrimary (citable) accession number: Q9WV55
Secondary accession number(s): Q3TJM1, Q9QY77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: January 9, 2007
Last modified: November 30, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.