UniProtKB - Q9WV48 (SHAN1_RAT)
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Protein
SH3 and multiple ankyrin repeat domains protein 1
Gene
Shank1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction. Overexpression promotes maturation of dendritic spines and the enlargement of spine heads via its ability to recruit Homer to postsynaptic sites, and enhances presynaptic function.2 Publications
GO - Molecular functioni
- ankyrin repeat binding Source: BHF-UCL
- GKAP/Homer scaffold activity Source: BHF-UCL
- identical protein binding Source: BHF-UCL
- ionotropic glutamate receptor binding Source: RGD
- protein complex binding Source: RGD
- protein C-terminus binding Source: RGD
- receptor signaling complex scaffold activity Source: RGD
- scaffold protein binding Source: BHF-UCL
- SH3 domain binding Source: BHF-UCL
- somatostatin receptor binding Source: UniProtKB
GO - Biological processi
- adult behavior Source: RGD
- associative learning Source: BHF-UCL
- cytoskeletal anchoring at plasma membrane Source: UniProtKB
- dendritic spine morphogenesis Source: BHF-UCL
- determination of affect Source: RGD
- habituation Source: BHF-UCL
- long-term memory Source: BHF-UCL
- negative regulation of actin filament bundle assembly Source: BHF-UCL
- neuromuscular process controlling balance Source: BHF-UCL
- olfactory behavior Source: BHF-UCL
- positive regulation of dendritic spine development Source: UniProtKB
- positive regulation of excitatory postsynaptic potential Source: BHF-UCL
- protein complex assembly Source: BHF-UCL
- protein localization to synapse Source: BHF-UCL
- regulation of AMPA receptor activity Source: BHF-UCL
- righting reflex Source: BHF-UCL
- social behavior Source: RGD
- synapse maturation Source: UniProtKB
- vocalization behavior Source: RGD
Keywordsi
Biological process | Differentiation, Neurogenesis |
Enzyme and pathway databases
Reactomei | R-RNO-6794361. Neurexins and neuroligins. |
Names & Taxonomyi
Protein namesi | Recommended name: SH3 and multiple ankyrin repeat domains protein 1Short name: Shank1 Alternative name(s): GKAP/SAPAP-interacting protein SPANK-1 Somatostatin receptor-interacting protein Short name: SSTR-interacting protein Short name: SSTRIP Synamon |
Gene namesi | Name:Shank1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621011. Shank1. |
Subcellular locationi
Keywords - Cellular componenti
Cell junction, Cell membrane, Cytoplasm, Membrane, Postsynaptic cell membrane, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000174672 | 1 – 2167 | SH3 and multiple ankyrin repeat domains protein 1Add BLAST | 2167 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Modified residuei | 43 | Omega-N-methylarginineBy similarity | 1 | ||
Modified residuei | 186 | PhosphotyrosineBy similarity | 1 | ||
Modified residuei | 540 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 544 | Omega-N-methylarginineBy similarity | 1 | ||
Modified residuei | 671 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 791 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 898 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 958 | Omega-N-methylarginineBy similarity | 1 | ||
Modified residuei | 1059 | Omega-N-methylarginineBy similarity | 1 | ||
Modified residuei | 1098 | Omega-N-methylarginineBy similarity | 1 | ||
Modified residuei | 1109 | Omega-N-methylarginineBy similarity | 1 | ||
Modified residuei | 1257 | Asymmetric dimethylarginineBy similarity | 1 | ||
Modified residuei | 1291 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 1429 | Omega-N-methylarginineBy similarity | 1 | ||
Modified residuei | 1442 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 1901 | Omega-N-methylarginineBy similarity | 1 | ||
Modified residuei | 2022 | Omega-N-methylarginineBy similarity | 1 | ||
Modified residuei | 2042 | Omega-N-methylarginineBy similarity | 1 | ||
Modified residuei | 2080 | Omega-N-methylarginineBy similarity | 1 | ||
Isoform 3 (identifier: Q9WV48-3) | |||||
Modified residuei | 638 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 641 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
PaxDbi | Q9WV48. |
PRIDEi | Q9WV48. |
PTM databases
iPTMneti | Q9WV48. |
PhosphoSitePlusi | Q9WV48. |
Expressioni
Tissue specificityi
Expressed only in brain (neuropil of cortex, CA1 region hippocampus and molecular layer of cerebellum).
Developmental stagei
Expression increases from low levels at birth to high levels at 3-4 weeks before dropping slightly in adulthood. Expressed in the cortex and the molecular layer of the cerebellum at postnatal day 7. Isoform 2 expression does not change during development of both cortex and cerebellum. Isoform 4 expression decreases significantly during development of cortex but not cerebellum.1 Publication
Gene expression databases
Bgeei | ENSRNOG00000019207. |
Genevisiblei | Q9WV48. RN. |
Interactioni
Subunit structurei
May homomultimerize via its SAM domain. Interacts with the C-terminus of SSTR2 via the PDZ domain. Interacts with SHARPIN, SPTAN1, HOMER1 and DLGAP1/GKAP. Part of a complex with DLG4/PSD-95 and DLGAP1/GKAP. Interacts with BAIAP2 (By similarity). Interacts with IGSF9.By similarity7 Publications
Binary interactionsi
GO - Molecular functioni
- ankyrin repeat binding Source: BHF-UCL
- GKAP/Homer scaffold activity Source: BHF-UCL
- identical protein binding Source: BHF-UCL
- ionotropic glutamate receptor binding Source: RGD
- protein complex binding Source: RGD
- protein C-terminus binding Source: RGD
- receptor signaling complex scaffold activity Source: RGD
- scaffold protein binding Source: BHF-UCL
- SH3 domain binding Source: BHF-UCL
- somatostatin receptor binding Source: UniProtKB
Protein-protein interaction databases
BioGridi | 249365. 5 interactors. |
CORUMi | Q9WV48. |
ELMi | Q9WV48. |
IntActi | Q9WV48. 17 interactors. |
MINTi | Q9WV48. |
STRINGi | 10116.ENSRNOP00000026100. |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more detailsFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Beta strandi | 657 – 667 | Combined sources | 11 | |
Beta strandi | 670 – 672 | Combined sources | 3 | |
Beta strandi | 675 – 682 | Combined sources | 8 | |
Beta strandi | 694 – 696 | Combined sources | 3 | |
Beta strandi | 698 – 705 | Combined sources | 8 | |
Helixi | 710 – 713 | Combined sources | 4 | |
Beta strandi | 721 – 725 | Combined sources | 5 | |
Helixi | 735 – 744 | Combined sources | 10 | |
Turni | 745 – 747 | Combined sources | 3 | |
Beta strandi | 748 – 757 | Combined sources | 10 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1Q3O | X-ray | 1.80 | A/B | 654-762 | [»] | |
1Q3P | X-ray | 2.25 | A/B | 654-762 | [»] | |
3L4F | X-ray | 2.80 | D | 653-765 | [»] | |
3QJM | X-ray | 2.31 | A/B | 654-768 | [»] | |
3QJN | X-ray | 2.71 | A/B/C/D/E/F/G/H | 654-768 | [»] | |
ProteinModelPortali | Q9WV48. | |||||
SMRi | Q9WV48. | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9WV48. |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 195 – 210 | ANK 1Add BLAST | 16 | |
Repeati | 212 – 245 | ANK 2Add BLAST | 34 | |
Repeati | 246 – 278 | ANK 3Add BLAST | 33 | |
Repeati | 279 – 312 | ANK 4Add BLAST | 34 | |
Repeati | 313 – 345 | ANK 5Add BLAST | 33 | |
Repeati | 346 – 378 | ANK 6Add BLAST | 33 | |
Repeati | 379 – 395 | ANK 7Add BLAST | 17 | |
Domaini | 554 – 613 | SH3PROSITE-ProRule annotationAdd BLAST | 60 | |
Domaini | 663 – 757 | PDZPROSITE-ProRule annotationAdd BLAST | 95 | |
Domaini | 2104 – 2167 | SAMPROSITE-ProRule annotationAdd BLAST | 64 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 929 – 932 | Poly-Pro | 4 | |
Compositional biasi | 1010 – 1015 | Poly-His | 6 | |
Compositional biasi | 1022 – 1027 | Poly-His | 6 | |
Compositional biasi | 1194 – 1199 | Poly-Gly | 6 | |
Compositional biasi | 1850 – 1860 | Poly-ProAdd BLAST | 11 |
Sequence similaritiesi
Belongs to the SHANK family.Curated
Keywords - Domaini
ANK repeat, Repeat, SH3 domainPhylogenomic databases
eggNOGi | KOG0504. Eukaryota. KOG4375. Eukaryota. COG0666. LUCA. |
GeneTreei | ENSGT00510000046474. |
HOGENOMi | HOG000293276. |
HOVERGENi | HBG079186. |
InParanoidi | Q9WV48. |
KOi | K15009. |
OMAi | DHPLETI. |
OrthoDBi | EOG091G00OW. |
PhylomeDBi | Q9WV48. |
Family and domain databases
CDDi | cd00204. ANK. 2 hits. cd11982. SH3_Shank1. 1 hit. |
Gene3Di | 1.25.40.20. 2 hits. |
InterProi | View protein in InterPro IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR036770. Ankyrin_rpt-contain_sf. IPR032425. FERM_f0. IPR001478. PDZ. IPR036034. PDZ_sf. IPR001660. SAM. IPR013761. SAM/pointed_sf. IPR036028. SH3-like_dom_sf. IPR001452. SH3_domain. IPR035735. Shank1_SH3. |
Pfami | View protein in Pfam PF12796. Ank_2. 2 hits. PF16511. FERM_f0. 1 hit. PF00595. PDZ. 1 hit. PF00536. SAM_1. 1 hit. PF07653. SH3_2. 1 hit. |
SMARTi | View protein in SMART SM00248. ANK. 6 hits. SM00228. PDZ. 1 hit. SM00454. SAM. 1 hit. SM00326. SH3. 1 hit. |
SUPFAMi | SSF47769. SSF47769. 1 hit. SSF48403. SSF48403. 1 hit. SSF50044. SSF50044. 1 hit. SSF50156. SSF50156. 1 hit. |
PROSITEi | View protein in PROSITE PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 3 hits. PS50106. PDZ. 1 hit. PS50105. SAM_DOMAIN. 1 hit. PS50002. SH3. 1 hit. |
s (5)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basket
Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q9WV48-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MTHSPATSED EERHSASECP EGGSESDSSP DGPGRGPQGT RGRGSGAPGN
60 70 80 90 100
LASTRGLQGR SMSVPDDAHF SMMVFRIGIP DLHQTKCLRF NPDATIWTAK
110 120 130 140 150
QQVLCALSES LQDVLNYGLF QPATSGRDAN FLEEERLLRE YPQSFEKGVP
160 170 180 190 200
YLEFRYKTRV YKQTNLDEKQ LAKLHTKTGL KKFLEYVQLG TSDKVARLLD
210 220 230 240 250
KGLDPNYHDS DSGETPLTLA AQTEGSVEVI RTLCLGGAHI DFRARDGMTA
260 270 280 290 300
LHKAACARHC LALTALLDLG GSPNYKDRRG LTPLFHTAMV GGDPRCCELL
310 320 330 340 350
LYNRAQLGIA DENGWQEIHQ ACQRGHSQHL EHLLFYGAEP GAQNASGNTA
360 370 380 390 400
LHICALYNKE TCARILLYRG ANKDVKNNNG QTPFQVAVIA GNFELGELIR
410 420 430 440 450
NHREQDVVPF QESPKYAARR RGPPGAGLTV PPALLRANSD TSMALPDWMV
460 470 480 490 500
FSAPGASSSG TPGPTSGPQG QSQPSAPSTK LSSGTLRSAS SPRGARARSP
510 520 530 540 550
SRGRHPEDAK RQPRGRPSSS GTPRDGPAGG TGGSGGPGGS LGSRGRRRKL
560 570 580 590 600
YSAVPGRSFM AVKSYQAQGE GEISLSKGEK IKVLSIGEGG FWEGQVKGRV
610 620 630 640 650
GWFPSDCLEE VANRSQEGRQ ESRSDKAKRL FRHYTVGSYD SFDAPSLIDG
660 670 680 690 700
IDSGSDYIIK EKTVLLQKKD SEGFGFVLRG AKAQTPIEEF TPTPAFPALQ
710 720 730 740 750
YLESVDEGGV AWRAGLRMGD FLIEVNGQNV VKVGHRQVVN MIRQGGNTLM
760 770 780 790 800
VKVVMVTRHP DMDEAVHKKA SQQAKRLPPP AISLRSKSMT SELEEMVSPW
810 820 830 840 850
KKKIEYEQQP AAVPSMEKKR TVYQMALNKL DEILAAAQQT ISASESPGPG
860 870 880 890 900
GLASLGKHRP KGFFATESSF DPHHRSQPSY DRPSFLPPGP GLMLRQKSIG
910 920 930 940 950
AAEDDRPYLA PPAMKFSRSL SVPGSEDIPP PPTTSPPEPP YSTPPAPSSS
960 970 980 990 1000
GRLTPSPRGG PFNPSSGGPL PASSPSSFDG PSPPDTRGGG REKSLYHSAA
1010 1020 1030 1040 1050
LPPAHHHPPH HHHHHAPPPQ PHHHHAHPPH PPEMETGGSP DDPPPRLALG
1060 1070 1080 1090 1100
PQPSLRGWRG GGPSPTSGAP SPSHHSSSGG SSGPTQAPAL RYFQLPPRAA
1110 1120 1130 1140 1150
SAAMYVPARS GRGRKGPLVK QTKVEGEPQK GSIPSASSPT SPALPRSEPP
1160 1170 1180 1190 1200
PAGPSEKNSI PIPTIIIKAP STSSSGRSSQ GSSTEAEPPT QPDGAGGGGS
1210 1220 1230 1240 1250
SPSPAPATSP VPPSPSPVPT PASPSGPATL DFTSQFGAAL VGAARREGGW
1260 1270 1280 1290 1300
QNEARRRSTL FLSTDAGDED GGDSGLGPGG PPGPRLRHSK SIDEGMFSAE
1310 1320 1330 1340 1350
PYLRLESGGS SGGYGAYAAG SRAYGGSGSS SAFTSFLPPR PLVHPLTGKA
1360 1370 1380 1390 1400
LDPASPLGLA LAARERALKE SSEGGGTPQP PPRPPSPRYD APPPTLHHHS
1410 1420 1430 1440 1450
PHSPHSPHAR HEPVLRLWGD PARRELGYRA GLGSQEKALT ASPPAARRSL
1460 1470 1480 1490 1500
LHRLPPTAPG VGPLLLQLGP EPPTPHPGVS KAWRTAAPEE PERLPLHVRF
1510 1520 1530 1540 1550
LENCQARPPP AGTRGSSTED GPGVPPPSPR RVLPTSPTSP RGNEENGLPL
1560 1570 1580 1590 1600
LVLPPPAPSV DVDDGEFLFA EPLPPPLEFS NSFEKPESPL TPGPPHPLPD
1610 1620 1630 1640 1650
PPSPATPLPA APPPAVAAAP PTLDSTASSL TSYDSEVATL TQGAPAAPGD
1660 1670 1680 1690 1700
PPAPGPPAPA APAPPAPQPG PDPPPGTDSG IEEVDSRSSS DHPLETISSA
1710 1720 1730 1740 1750
STLSSLSAEG GGNTGGVAGG GAGVASGTEL LDTYVAYLDG QAFGGSGTPG
1760 1770 1780 1790 1800
PPYPPQLMTP SKLRGRALGT SGNLRPGPSG GLRDPVTPTS PTVSVTGAGT
1810 1820 1830 1840 1850
DGLLALSACP GPSTAGVAGG PVAVEPEVPP VPLPAASSLP RKLLPWEEGP
1860 1870 1880 1890 1900
GPPPPPLPGP LSQPQASALA TVKASIISEL SSKLQQFGGS STAGGALPWA
1910 1920 1930 1940 1950
RGGSGGSTDS HHGGASYIPE RTSSLQRQRL SEDSQTSLLS KPSSSIFQNW
1960 1970 1980 1990 2000
PKPPLPPLPT GSGVSSSTAA APGATSPSAS SASASTRHLQ GVEFEMRPPL
2010 2020 2030 2040 2050
LRRAPSPSLL PASDHKVSPA PRPSSLPILP SGPIYPGLFD IRSSPTGGAG
2060 2070 2080 2090 2100
GSTDPFAPVF VPPHPGISGG LGGALSGASR SLSPTRLLSL PPDKPFGAKP
2110 2120 2130 2140 2150
LGFWTKFDVA DWLEWLGLSE HRAQFLDHEI DGSHLPALTK EDYVDLGVTR
2160
VGHRMNIDRA LKFFLER
Sequence cautioni
The sequence AAD29417 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAF02498 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1141 | S → T in AAD04569 (PubMed:10488079).Curated | 1 | |
Sequence conflicti | 1174 | S → N in AAD29417 (PubMed:10433268).Curated | 1 | |
Sequence conflicti | 1246 | R → K in AAD04569 (PubMed:10488079).Curated | 1 | |
Sequence conflicti | 1323 | A → T in AAD04569 (PubMed:10488079).Curated | 1 | |
Sequence conflicti | 1331 | S → D in AAD04569 (PubMed:10488079).Curated | 1 | |
Sequence conflicti | 1726 | S → N in AAD29417 (PubMed:10433268).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_006072 | 1 – 614 | Missing in isoform 2. CuratedAdd BLAST | 614 | |
Alternative sequenceiVSP_006073 | 615 – 654 | SQEGR…GIDSG → MALSAVGGGPGGGALPQPPP ALSSSWPALGPRRRSVWYIY in isoform 2. CuratedAdd BLAST | 40 | |
Alternative sequenceiVSP_006074 | 646 – 654 | Missing in isoform 3. 1 Publication | 9 | |
Alternative sequenceiVSP_006075 | 797 – 804 | Missing in isoform 4. 1 Publication | 8 | |
Alternative sequenceiVSP_006076 | 1930 – 1943 | LSEDS…LSKPS → QYRIVVKSSDFGDF in isoform 5. CuratedAdd BLAST | 14 | |
Alternative sequenceiVSP_006077 | 1944 – 2167 | Missing in isoform 5. CuratedAdd BLAST | 224 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF102855 mRNA. Translation: AAD04569.2. AF131951 mRNA. Translation: AAD29417.1. Different initiation. AF159046 mRNA. Translation: AAD42975.1. AF141904 mRNA. Translation: AAF02498.1. Different initiation. |
RefSeqi | NP_113939.2. NM_031751.3. [Q9WV48-1] XP_017445247.1. XM_017589758.1. [Q9WV48-1] XP_017445248.1. XM_017589759.1. [Q9WV48-4] XP_017445249.1. XM_017589760.1. [Q9WV48-3] XP_017445250.1. XM_017589761.1. [Q9WV48-5] |
UniGenei | Rn.225968. |
Genome annotation databases
Ensembli | ENSRNOT00000026100; ENSRNOP00000026100; ENSRNOG00000019207. [Q9WV48-1] ENSRNOT00000044257; ENSRNOP00000039860; ENSRNOG00000019207. [Q9WV48-3] ENSRNOT00000092327; ENSRNOP00000075838; ENSRNOG00000019207. [Q9WV48-4] |
GeneIDi | 78957. |
KEGGi | rno:78957. |
UCSCi | RGD:621011. rat. [Q9WV48-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Entry informationi
Entry namei | SHAN1_RAT | |
Accessioni | Q9WV48Primary (citable) accession number: Q9WV48 Secondary accession number(s): Q9QZZ8, Q9WU13, Q9WUE8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 26, 2002 |
Last sequence update: | November 1, 1999 | |
Last modified: | March 28, 2018 | |
This is version 170 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |