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UniProtKB - Q9WV48 (SHAN1_RAT)

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Protein

SH3 and multiple ankyrin repeat domains protein 1

Gene

Shank1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction. Overexpression promotes maturation of dendritic spines and the enlargement of spine heads via its ability to recruit Homer to postsynaptic sites, and enhances presynaptic function.2 Publications

GO - Molecular functioni

  • ankyrin repeat binding Source: BHF-UCL
  • GKAP/Homer scaffold activity Source: BHF-UCL
  • identical protein binding Source: BHF-UCL
  • ionotropic glutamate receptor binding Source: RGD
  • protein complex binding Source: RGD
  • protein C-terminus binding Source: RGD
  • receptor signaling complex scaffold activity Source: RGD
  • scaffold protein binding Source: BHF-UCL
  • SH3 domain binding Source: BHF-UCL
  • somatostatin receptor binding Source: UniProtKB
Complete GO annotation...

GO - Biological processi

Complete GO annotation...

Keywordsi

Biological processDifferentiation, Neurogenesis

Enzyme and pathway databases

ReactomeiR-RNO-6794361. Interactions of neurexins and neuroligins at synapses.

Names & Taxonomyi

Protein namesi
Recommended name:
SH3 and multiple ankyrin repeat domains protein 1
Short name:
Shank1
Alternative name(s):
GKAP/SAPAP-interacting protein
SPANK-1
Somatostatin receptor-interacting protein
Short name:
SSTR-interacting protein
Short name:
SSTRIP
Synamon
Gene namesi
Name:Shank1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi621011. Shank1.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cytosol Source: Reactome
  • dendrite Source: UniProtKB
  • dendritic spine Source: BHF-UCL
  • excitatory synapse Source: BHF-UCL
  • intracellular Source: UniProtKB
  • ionotropic glutamate receptor complex Source: Ensembl
  • neuron projection Source: BHF-UCL
  • plasma membrane Source: BHF-UCL
  • postsynaptic density Source: BHF-UCL
  • postsynaptic membrane Source: RGD
  • synapse Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001746721 – 2167SH3 and multiple ankyrin repeat domains protein 1Add BLAST2167

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei43Omega-N-methylarginineBy similarity1
Modified residuei186PhosphotyrosineBy similarity1
Modified residuei540PhosphoserineCombined sources1
Modified residuei544Omega-N-methylarginineBy similarity1
Modified residuei671PhosphoserineBy similarity1
Modified residuei791PhosphoserineBy similarity1
Modified residuei898PhosphoserineCombined sources1
Modified residuei958Omega-N-methylarginineBy similarity1
Modified residuei1059Omega-N-methylarginineBy similarity1
Modified residuei1098Omega-N-methylarginineBy similarity1
Modified residuei1109Omega-N-methylarginineBy similarity1
Modified residuei1257Asymmetric dimethylarginineBy similarity1
Modified residuei1291PhosphoserineCombined sources1
Modified residuei1429Omega-N-methylarginineBy similarity1
Modified residuei1442PhosphoserineCombined sources1
Modified residuei1901Omega-N-methylarginineBy similarity1
Modified residuei2022Omega-N-methylarginineBy similarity1
Modified residuei2042Omega-N-methylarginineBy similarity1
Modified residuei2080Omega-N-methylarginineBy similarity1
Isoform 3 (identifier: Q9WV48-3)
Modified residuei638PhosphoserineCombined sources1
Modified residuei641PhosphoserineCombined sources1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ9WV48.
PRIDEiQ9WV48.

PTM databases

iPTMnetiQ9WV48.
PhosphoSitePlusiQ9WV48.

Expressioni

Tissue specificityi

Expressed only in brain (neuropil of cortex, CA1 region hippocampus and molecular layer of cerebellum).

Developmental stagei

Expression increases from low levels at birth to high levels at 3-4 weeks before dropping slightly in adulthood. Expressed in the cortex and the molecular layer of the cerebellum at postnatal day 7. Isoform 2 expression does not change during development of both cortex and cerebellum. Isoform 4 expression decreases significantly during development of cortex but not cerebellum.1 Publication

Gene expression databases

BgeeiENSRNOG00000019207.
GenevisibleiQ9WV48. RN.

Interactioni

Subunit structurei

May homomultimerize via its SAM domain. Interacts with the C-terminus of SSTR2 via the PDZ domain. Interacts with SHARPIN, SPTAN1, HOMER1 and DLGAP1/GKAP. Part of a complex with DLG4/PSD-95 and DLGAP1/GKAP. Interacts with BAIAP2 (By similarity). Interacts with IGSF9.By similarity7 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • ankyrin repeat binding Source: BHF-UCL
  • GKAP/Homer scaffold activity Source: BHF-UCL
  • identical protein binding Source: BHF-UCL
  • ionotropic glutamate receptor binding Source: RGD
  • protein complex binding Source: RGD
  • protein C-terminus binding Source: RGD
  • receptor signaling complex scaffold activity Source: RGD
  • scaffold protein binding Source: BHF-UCL
  • SH3 domain binding Source: BHF-UCL
  • somatostatin receptor binding Source: UniProtKB
Complete GO annotation...

Protein-protein interaction databases

BioGridi249365. 5 interactors.
IntActiQ9WV48. 17 interactors.
MINTiMINT-101286.
STRINGi10116.ENSRNOP00000026100.

Structurei

Secondary structure

12167
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi657 – 667Combined sources11
Beta strandi670 – 672Combined sources3
Beta strandi675 – 682Combined sources8
Beta strandi694 – 696Combined sources3
Beta strandi698 – 705Combined sources8
Helixi710 – 713Combined sources4
Beta strandi721 – 725Combined sources5
Helixi735 – 744Combined sources10
Turni745 – 747Combined sources3
Beta strandi748 – 757Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Q3OX-ray1.80A/B654-762[»]
1Q3PX-ray2.25A/B654-762[»]
3L4FX-ray2.80D653-765[»]
3QJMX-ray2.31A/B654-768[»]
3QJNX-ray2.71A/B/C/D/E/F/G/H654-768[»]
ProteinModelPortaliQ9WV48.
SMRiQ9WV48.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WV48.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati195 – 210ANK 1Add BLAST16
Repeati212 – 245ANK 2Add BLAST34
Repeati246 – 278ANK 3Add BLAST33
Repeati279 – 312ANK 4Add BLAST34
Repeati313 – 345ANK 5Add BLAST33
Repeati346 – 378ANK 6Add BLAST33
Repeati379 – 395ANK 7Add BLAST17
Domaini554 – 613SH3PROSITE-ProRule annotationAdd BLAST60
Domaini663 – 757PDZPROSITE-ProRule annotationAdd BLAST95
Domaini2104 – 2167SAMPROSITE-ProRule annotationAdd BLAST64

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi929 – 932Poly-Pro4
Compositional biasi1010 – 1015Poly-His6
Compositional biasi1022 – 1027Poly-His6
Compositional biasi1194 – 1199Poly-Gly6
Compositional biasi1850 – 1860Poly-ProAdd BLAST11

Sequence similaritiesi

Belongs to the SHANK family.Curated

Keywords - Domaini

ANK repeat, Repeat, SH3 domain

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
KOG4375. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00510000046474.
HOGENOMiHOG000293276.
HOVERGENiHBG079186.
InParanoidiQ9WV48.
KOiK15009.
OMAiDHPLETI.
OrthoDBiEOG091G00OW.
PhylomeDBiQ9WV48.

Family and domain databases

CDDicd00204. ANK. 2 hits.
Gene3Di1.25.40.20. 2 hits.
InterProiView protein in InterPro
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR032425. FERM_f0.
IPR001478. PDZ.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR011511. SH3_2.
IPR001452. SH3_domain.
PfamiView protein in Pfam
PF12796. Ank_2. 2 hits.
PF16511. FERM_f0. 1 hit.
PF00595. PDZ. 1 hit.
PF00536. SAM_1. 1 hit.
PF07653. SH3_2. 1 hit.
SMARTiView protein in SMART
SM00248. ANK. 6 hits.
SM00228. PDZ. 1 hit.
SM00454. SAM. 1 hit.
SM00326. SH3. 1 hit.
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF48403. SSF48403. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50156. SSF50156. 1 hit.
PROSITEiView protein in PROSITE
PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS50106. PDZ. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q9WV48-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTHSPATSED EERHSASECP EGGSESDSSP DGPGRGPQGT RGRGSGAPGN 
        60         70         80         90        100
LASTRGLQGR SMSVPDDAHF SMMVFRIGIP DLHQTKCLRF NPDATIWTAK 
       110        120        130        140        150
QQVLCALSES LQDVLNYGLF QPATSGRDAN FLEEERLLRE YPQSFEKGVP 
       160        170        180        190        200
YLEFRYKTRV YKQTNLDEKQ LAKLHTKTGL KKFLEYVQLG TSDKVARLLD 
       210        220        230        240        250
KGLDPNYHDS DSGETPLTLA AQTEGSVEVI RTLCLGGAHI DFRARDGMTA 
       260        270        280        290        300
LHKAACARHC LALTALLDLG GSPNYKDRRG LTPLFHTAMV GGDPRCCELL 
       310        320        330        340        350
LYNRAQLGIA DENGWQEIHQ ACQRGHSQHL EHLLFYGAEP GAQNASGNTA 
       360        370        380        390        400
LHICALYNKE TCARILLYRG ANKDVKNNNG QTPFQVAVIA GNFELGELIR 
       410        420        430        440        450
NHREQDVVPF QESPKYAARR RGPPGAGLTV PPALLRANSD TSMALPDWMV 
       460        470        480        490        500
FSAPGASSSG TPGPTSGPQG QSQPSAPSTK LSSGTLRSAS SPRGARARSP 
       510        520        530        540        550
SRGRHPEDAK RQPRGRPSSS GTPRDGPAGG TGGSGGPGGS LGSRGRRRKL 
       560        570        580        590        600
YSAVPGRSFM AVKSYQAQGE GEISLSKGEK IKVLSIGEGG FWEGQVKGRV 
       610        620        630        640        650
GWFPSDCLEE VANRSQEGRQ ESRSDKAKRL FRHYTVGSYD SFDAPSLIDG 
       660        670        680        690        700
IDSGSDYIIK EKTVLLQKKD SEGFGFVLRG AKAQTPIEEF TPTPAFPALQ 
       710        720        730        740        750
YLESVDEGGV AWRAGLRMGD FLIEVNGQNV VKVGHRQVVN MIRQGGNTLM 
       760        770        780        790        800
VKVVMVTRHP DMDEAVHKKA SQQAKRLPPP AISLRSKSMT SELEEMVSPW 
       810        820        830        840        850
KKKIEYEQQP AAVPSMEKKR TVYQMALNKL DEILAAAQQT ISASESPGPG 
       860        870        880        890        900
GLASLGKHRP KGFFATESSF DPHHRSQPSY DRPSFLPPGP GLMLRQKSIG 
       910        920        930        940        950
AAEDDRPYLA PPAMKFSRSL SVPGSEDIPP PPTTSPPEPP YSTPPAPSSS 
       960        970        980        990       1000
GRLTPSPRGG PFNPSSGGPL PASSPSSFDG PSPPDTRGGG REKSLYHSAA 
      1010       1020       1030       1040       1050
LPPAHHHPPH HHHHHAPPPQ PHHHHAHPPH PPEMETGGSP DDPPPRLALG 
      1060       1070       1080       1090       1100
PQPSLRGWRG GGPSPTSGAP SPSHHSSSGG SSGPTQAPAL RYFQLPPRAA 
      1110       1120       1130       1140       1150
SAAMYVPARS GRGRKGPLVK QTKVEGEPQK GSIPSASSPT SPALPRSEPP 
      1160       1170       1180       1190       1200
PAGPSEKNSI PIPTIIIKAP STSSSGRSSQ GSSTEAEPPT QPDGAGGGGS 
      1210       1220       1230       1240       1250
SPSPAPATSP VPPSPSPVPT PASPSGPATL DFTSQFGAAL VGAARREGGW 
      1260       1270       1280       1290       1300
QNEARRRSTL FLSTDAGDED GGDSGLGPGG PPGPRLRHSK SIDEGMFSAE 
      1310       1320       1330       1340       1350
PYLRLESGGS SGGYGAYAAG SRAYGGSGSS SAFTSFLPPR PLVHPLTGKA 
      1360       1370       1380       1390       1400
LDPASPLGLA LAARERALKE SSEGGGTPQP PPRPPSPRYD APPPTLHHHS 
      1410       1420       1430       1440       1450
PHSPHSPHAR HEPVLRLWGD PARRELGYRA GLGSQEKALT ASPPAARRSL 
      1460       1470       1480       1490       1500
LHRLPPTAPG VGPLLLQLGP EPPTPHPGVS KAWRTAAPEE PERLPLHVRF 
      1510       1520       1530       1540       1550
LENCQARPPP AGTRGSSTED GPGVPPPSPR RVLPTSPTSP RGNEENGLPL 
      1560       1570       1580       1590       1600
LVLPPPAPSV DVDDGEFLFA EPLPPPLEFS NSFEKPESPL TPGPPHPLPD 
      1610       1620       1630       1640       1650
PPSPATPLPA APPPAVAAAP PTLDSTASSL TSYDSEVATL TQGAPAAPGD 
      1660       1670       1680       1690       1700
PPAPGPPAPA APAPPAPQPG PDPPPGTDSG IEEVDSRSSS DHPLETISSA 
      1710       1720       1730       1740       1750
STLSSLSAEG GGNTGGVAGG GAGVASGTEL LDTYVAYLDG QAFGGSGTPG 
      1760       1770       1780       1790       1800
PPYPPQLMTP SKLRGRALGT SGNLRPGPSG GLRDPVTPTS PTVSVTGAGT 
      1810       1820       1830       1840       1850
DGLLALSACP GPSTAGVAGG PVAVEPEVPP VPLPAASSLP RKLLPWEEGP 
      1860       1870       1880       1890       1900
GPPPPPLPGP LSQPQASALA TVKASIISEL SSKLQQFGGS STAGGALPWA 
      1910       1920       1930       1940       1950
RGGSGGSTDS HHGGASYIPE RTSSLQRQRL SEDSQTSLLS KPSSSIFQNW 
      1960       1970       1980       1990       2000
PKPPLPPLPT GSGVSSSTAA APGATSPSAS SASASTRHLQ GVEFEMRPPL 
      2010       2020       2030       2040       2050
LRRAPSPSLL PASDHKVSPA PRPSSLPILP SGPIYPGLFD IRSSPTGGAG 
      2060       2070       2080       2090       2100
GSTDPFAPVF VPPHPGISGG LGGALSGASR SLSPTRLLSL PPDKPFGAKP 
      2110       2120       2130       2140       2150
LGFWTKFDVA DWLEWLGLSE HRAQFLDHEI DGSHLPALTK EDYVDLGVTR 
      2160 
VGHRMNIDRA LKFFLER
Length:2,167
Mass (Da):226,335
Last modified:November 1, 1999 - v1
Checksum:i3F478B5A7B18BA86
GO
Isoform 2 (identifier: Q9WV48-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-614: Missing.
     615-654: SQEGRQESRS...PSLIDGIDSG → MALSAVGGGP...PRRRSVWYIY

Show »
Length:1,553
Mass (Da):159,899
Checksum:iB9B7E6A33E26DE48
GO
Isoform 3 (identifier: Q9WV48-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     646-654: Missing.

Show »
Length:2,158
Mass (Da):225,477
Checksum:iBF18578E26D6578C
GO
Isoform 4 (identifier: Q9WV48-4) [UniParc]FASTAAdd to basket
Also known as: A

The sequence of this isoform differs from the canonical sequence as follows:
     797-804: Missing.

Show »
Length:2,159
Mass (Da):225,368
Checksum:i912C1737EC330CB1
GO
Isoform 5 (identifier: Q9WV48-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1930-1943: LSEDSQTSLLSKPS → QYRIVVKSSDFGDF
     1944-2167: Missing.

Show »
Length:1,943
Mass (Da):202,919
Checksum:iD290DD45558B73AF
GO

Sequence cautioni

The sequence AAD29417 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAF02498 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1141S → T in AAD04569 (PubMed:10488079).Curated1
Sequence conflicti1174S → N in AAD29417 (PubMed:10433268).Curated1
Sequence conflicti1246R → K in AAD04569 (PubMed:10488079).Curated1
Sequence conflicti1323A → T in AAD04569 (PubMed:10488079).Curated1
Sequence conflicti1331S → D in AAD04569 (PubMed:10488079).Curated1
Sequence conflicti1726S → N in AAD29417 (PubMed:10433268).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0060721 – 614Missing in isoform 2. CuratedAdd BLAST614
Alternative sequenceiVSP_006073615 – 654SQEGR…GIDSG → MALSAVGGGPGGGALPQPPP ALSSSWPALGPRRRSVWYIY in isoform 2. CuratedAdd BLAST40
Alternative sequenceiVSP_006074646 – 654Missing in isoform 3. 1 Publication9
Alternative sequenceiVSP_006075797 – 804Missing in isoform 4. 1 Publication8
Alternative sequenceiVSP_0060761930 – 1943LSEDS…LSKPS → QYRIVVKSSDFGDF in isoform 5. CuratedAdd BLAST14
Alternative sequenceiVSP_0060771944 – 2167Missing in isoform 5. CuratedAdd BLAST224

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF102855 mRNA. Translation: AAD04569.2.
AF131951 mRNA. Translation: AAD29417.1. Different initiation.
AF159046 mRNA. Translation: AAD42975.1.
AF141904 mRNA. Translation: AAF02498.1. Different initiation.
RefSeqiNP_113939.2. NM_031751.3. [Q9WV48-1]
XP_017445247.1. XM_017589758.1. [Q9WV48-1]
XP_017445248.1. XM_017589759.1. [Q9WV48-4]
XP_017445249.1. XM_017589760.1. [Q9WV48-3]
XP_017445250.1. XM_017589761.1. [Q9WV48-5]
UniGeneiRn.225968.

Genome annotation databases

EnsembliENSRNOT00000026100; ENSRNOP00000026100; ENSRNOG00000019207. [Q9WV48-1]
ENSRNOT00000044257; ENSRNOP00000039860; ENSRNOG00000019207. [Q9WV48-3]
ENSRNOT00000092327; ENSRNOP00000075838; ENSRNOG00000019207. [Q9WV48-4]
GeneIDi78957.
KEGGirno:78957.
UCSCiRGD:621011. rat. [Q9WV48-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiSHAN1_RAT
AccessioniPrimary (citable) accession number: Q9WV48
Secondary accession number(s): Q9QZZ8, Q9WU13, Q9WUE8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: November 1, 1999
Last modified: July 5, 2017
This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families