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Protein

Solute carrier family 2, facilitated glucose transporter member 5

Gene

Slc2a5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a fructose transporter that has only low activity with other monosaccharides (PubMed:12031501, PubMed:19091748). Can mediate the uptake of deoxyglucose, but with low efficiency (By similarity). Essential for fructose uptake in the small intestine (PubMed:19091748, PubMed:26071406). Plays a role in the regulation of salt uptake and blood pressure in response to dietary fructose. Required for the development of high blood pressure in response to high dietary fructose intake (PubMed:19091748).By similarity3 Publications

Enzyme regulationi

Fructose uptake is inhibited by cytochalasin B.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei31FructoseBy similarity1
Binding sitei166FructoseBy similarity1
Binding sitei287FructoseBy similarity1
Binding sitei386FructoseBy similarity1

GO - Molecular functioni

  • fructose binding Source: UniProtKB
  • fructose transmembrane transporter activity Source: UniProtKB

GO - Biological processi

  • cellular response to fructose stimulus Source: UniProtKB
  • fructose import across plasma membrane Source: UniProtKB
  • regulation of systemic arterial blood pressure mediated by a chemical signal Source: UniProtKB
  • response to fructose Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Sugar transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-189200. Hexose transport.
R-MMU-428776. Class II GLUTs.
R-MMU-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 2, facilitated glucose transporter member 5
Alternative name(s):
Fructose transporter
Glucose transporter type 5, small intestine
Short name:
GLUT-5
Gene namesi
Name:Slc2a5
Synonyms:Glut5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1928369. Slc2a5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 17CytoplasmicBy similarityAdd BLAST17
Transmembranei18 – 38Helical; Name=1By similarityAdd BLAST21
Topological domaini39 – 67ExtracellularBy similarityAdd BLAST29
Transmembranei68 – 90Helical; Name=2By similarityAdd BLAST23
Topological domaini91 – 97CytoplasmicBy similarity7
Transmembranei98 – 118Helical; Name=3By similarityAdd BLAST21
Topological domaini119 – 125ExtracellularBy similarity7
Transmembranei126 – 148Helical; Name=4By similarityAdd BLAST23
Topological domaini149 – 160CytoplasmicBy similarityAdd BLAST12
Transmembranei161 – 181Helical; Name=5By similarityAdd BLAST21
Topological domaini182 – 191ExtracellularBy similarity10
Transmembranei192 – 212Helical; Name=6By similarityAdd BLAST21
Topological domaini213 – 276CytoplasmicBy similarityAdd BLAST64
Transmembranei277 – 297Helical; Name=7By similarityAdd BLAST21
Topological domaini298 – 312ExtracellularBy similarityAdd BLAST15
Transmembranei313 – 333Helical; Name=8By similarityAdd BLAST21
Topological domaini334 – 341CytoplasmicBy similarity8
Transmembranei342 – 362Helical; Name=9By similarityAdd BLAST21
Topological domaini363 – 370ExtracellularBy similarity8
Transmembranei371 – 393Helical; Name=10By similarityAdd BLAST23
Topological domaini394 – 411CytoplasmicBy similarityAdd BLAST18
Transmembranei412 – 432Helical; Name=11By similarityAdd BLAST21
Topological domaini433 – 438ExtracellularBy similarity6
Transmembranei439 – 459Helical; Name=12By similarityAdd BLAST21
Topological domaini460 – 501CytoplasmicBy similarityAdd BLAST42

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice fed a standard diet appear normal and healthy, and display no visible phenotype (PubMed:18417103, PubMed:19091748, PubMed:26071406). Mutant mice show reduced food intake when kept on a high-frucose diet and about 28% reduction of their body weight within seven days. They loose more weight than wild-type mice that receive similar amounts of high-fructose food (PubMed:19091748). Mutant mice show strongly decreased fructose absorption in the jejunum (PubMed:19091748, PubMed:26071406). Contrary to wild-type, mutant mice have a strongly distended colon and caecum when kept on a high-fructose diet. Their intestines look normal when they are fed a standard diet. Contrary to wild-type, mutant mice do not display increased fructose levels in blood serum when kept on a high-fructose diet. Contrary to wild-type, they do not show increased salt absorption in response to fructose, and do not develop high blood pressure in response to fructose feeding. On the contrary, the blood pressure of mutant mice is strongly decreased after five days on a high-fructose diet. Mutant mice develop hypovolemic shock and die after 7 to 10 days on high-fructose diet (PubMed:19091748). Mutant mice display no defects of cochlear morphology or any hearing defects (PubMed:18417103).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000503701 – 501Solute carrier family 2, facilitated glucose transporter member 5Add BLAST501

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Glycosylationi50N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Acetylation, Glycoprotein

Proteomic databases

PaxDbiQ9WV38.
PRIDEiQ9WV38.

PTM databases

iPTMnetiQ9WV38.
PhosphoSitePlusiQ9WV38.

Expressioni

Tissue specificityi

Detected at the apical membrane of villi in the jejunum (PubMed:18496516, PubMed:19091748, PubMed:26071406). Detected in jejunum mucosa (PubMed:26071406). Detected in epididymis and whole testis (at protein level) (PubMed:18417103). Detected in small intestine, kidney and testis (PubMed:12031501, PubMed:18417103, PubMed:19091748). Detected in cochlea, but not in inner or outer cochlear hair cells (PubMed:18417103).4 Publications

Inductioni

Up-regulated in jejunum by dietary fructose intake (at protein level) (PubMed:18496516). Up-regulated in jejunum by dietary fructose intake (PubMed:18496516, PubMed:26071406). Up-regulated by dietary fructose intake in small intestine and testis (PubMed:12031501).2 Publications

Gene expression databases

BgeeiENSMUSG00000028976.
GenevisibleiQ9WV38. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030826.

Structurei

3D structure databases

ProteinModelPortaliQ9WV38.
SMRiQ9WV38.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni295 – 297Fructose bindingBy similarity3
Regioni418 – 419Fructose bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0569. Eukaryota.
COG0477. LUCA.
GeneTreeiENSGT00760000119022.
HOGENOMiHOG000202871.
HOVERGENiHBG014816.
InParanoidiQ9WV38.
KOiK08143.
OMAiNTISWMP.
OrthoDBiEOG091G0A9K.
TreeFamiTF313762.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR002442. Fru_transpt_5.
IPR020846. MFS_dom.
IPR005828. MFS_sugar_transport-like.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSiPR01194. GLUCTRSPORT5.
PR00171. SUGRTRNSPORT.
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00879. SP. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WV38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEKHQEETG ELTLVLALAT LIAAFGSSFQ YGYNVAAVNS PSEFMQQFYN
60 70 80 90 100
DTYYDRNEEN IESFTLTLLW SLTVSMFPFG GFIGSLMVGT LVNKLGRKGA
110 120 130 140 150
LLFNNIFSIL PAILMGCSQI AQSFELIIIS RLLVGICAGI SSNVVPMYLG
160 170 180 190 200
ELAPKNLRGA LGVVPQLFIT VGILVAQLFG LRSLLANEDG WPVLLGLTGV
210 220 230 240 250
PAGLQLLLLP FFPESPRYLL IQKKDEAAAE RALQTLRGWK DVHLEMEEIR
260 270 280 290 300
KEDEAEKAAG FISVWKLFTM QSLRWQLISM IVLMAGQQLS GVNAIYYYAD
310 320 330 340 350
QIYLSAGVKS DDVQYVTAGT GAVNVFMTIL TIFVVELWGR RFLLLVGFST
360 370 380 390 400
CLIACLVLTA ALALQNTISW MPYISIVCVI VYVIGHALGP SPIPALLITE
410 420 430 440 450
IFLQSSRPAA YMIGGSVHWL SNFTVGLIFP FIQMGLGPYS FIIFATICFL
460 470 480 490 500
TTIYIFMVVP ETKGRTFIEI NQIFTMKNKV SDVYPKKEEE LGALPHAILE

Q
Length:501
Mass (Da):55,409
Last modified:July 27, 2011 - v2
Checksum:i4F562A3BF848E5C7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti84G → S in AAD42235 (PubMed:12031501).Curated1
Sequence conflicti87M → V in AAD42235 (PubMed:12031501).Curated1
Sequence conflicti90T → N in AAD42235 (PubMed:12031501).Curated1
Sequence conflicti97 – 99RKG → KKR in AAD42235 (PubMed:12031501).Curated3
Sequence conflicti114L → F in AAD42235 (PubMed:12031501).Curated1
Sequence conflicti236L → I in AAD42235 (PubMed:12031501).Curated1
Sequence conflicti471N → I in AAD42235 (PubMed:12031501).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF161071 mRNA. Translation: AAD42235.1.
AK029720 mRNA. Translation: BAC26582.1.
AL606971 Genomic DNA. Translation: CAM14288.1.
CU210939 Genomic DNA. Translation: CAQ51688.1.
CH466594 Genomic DNA. Translation: EDL14883.1.
BC023500 mRNA. Translation: AAH23500.1.
CCDSiCCDS18968.1.
RefSeqiNP_062715.2. NM_019741.3.
XP_006539139.1. XM_006539076.3.
XP_017175818.1. XM_017320329.1.
UniGeneiMm.260220.

Genome annotation databases

EnsembliENSMUST00000030826; ENSMUSP00000030826; ENSMUSG00000028976.
GeneIDi56485.
KEGGimmu:56485.
UCSCiuc008vxk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF161071 mRNA. Translation: AAD42235.1.
AK029720 mRNA. Translation: BAC26582.1.
AL606971 Genomic DNA. Translation: CAM14288.1.
CU210939 Genomic DNA. Translation: CAQ51688.1.
CH466594 Genomic DNA. Translation: EDL14883.1.
BC023500 mRNA. Translation: AAH23500.1.
CCDSiCCDS18968.1.
RefSeqiNP_062715.2. NM_019741.3.
XP_006539139.1. XM_006539076.3.
XP_017175818.1. XM_017320329.1.
UniGeneiMm.260220.

3D structure databases

ProteinModelPortaliQ9WV38.
SMRiQ9WV38.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030826.

PTM databases

iPTMnetiQ9WV38.
PhosphoSitePlusiQ9WV38.

Proteomic databases

PaxDbiQ9WV38.
PRIDEiQ9WV38.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030826; ENSMUSP00000030826; ENSMUSG00000028976.
GeneIDi56485.
KEGGimmu:56485.
UCSCiuc008vxk.1. mouse.

Organism-specific databases

CTDi6518.
MGIiMGI:1928369. Slc2a5.

Phylogenomic databases

eggNOGiKOG0569. Eukaryota.
COG0477. LUCA.
GeneTreeiENSGT00760000119022.
HOGENOMiHOG000202871.
HOVERGENiHBG014816.
InParanoidiQ9WV38.
KOiK08143.
OMAiNTISWMP.
OrthoDBiEOG091G0A9K.
TreeFamiTF313762.

Enzyme and pathway databases

ReactomeiR-MMU-189200. Hexose transport.
R-MMU-428776. Class II GLUTs.
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ9WV38.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028976.
GenevisibleiQ9WV38. MM.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR002442. Fru_transpt_5.
IPR020846. MFS_dom.
IPR005828. MFS_sugar_transport-like.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSiPR01194. GLUCTRSPORT5.
PR00171. SUGRTRNSPORT.
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00879. SP. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGTR5_MOUSE
AccessioniPrimary (citable) accession number: Q9WV38
Secondary accession number(s): Q8R1N7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.