Q9WV31 (ARC_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Activity-regulated cytoskeleton-associated protein

Alternative name(s):
ARC/ARG3.1
Short name=mArc
Activity-regulated gene 3.1 protein homolog
Short name=Arg3.1

Gene names

Name:

Arc

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration By similarity. Required for consolidation of synaptic plasticity as well as formation of long-term memory. Regulates endocytosis of AMPA receptors in response to synaptic activity. Required for homeostatic synaptic scaling of AMPA receptors. Ref.7 Ref.8
Subunit structure	Interacts with SH3GL1/endophilin-2, SH3GL3/endophilin-3 and DNM2/DYN2 By similarity. UniProtKB Q63053
Subcellular location	Cytoplasm › cytoskeleton By similarity. Endosome By similarity. Cytoplasmic vesicle › secretory vesicle › acrosome. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density By similarity. Cell projection › dendrite By similarity. Cell projection › dendritic spine By similarity. Cell junction › synapse By similarity. Note: Associated with the cell cortex of neuronal soma and dendrites. Enriched in postsynaptic density of dendritic spines. Enriched on the plasma membrane By similarity. Associated with the sperm tail. Ref.6
Tissue specificity	Expressed in brain and testis. Ref.6
Developmental stage	Detectable in brain from postnatal week 1, in testis from postnatal week 3. Ref.6
Disruption phenotype	Mice show deficits in several forms of long-term memory formation including spatial and fear-related learning, conditioned taste aversion as well as long-term object recognition. They show enhanced early-phase but impaired late-phase long-term potentiation (LTP) as well as impaired long-term depression (LTD). Neurons lacking Arc show an increase in surface levels of AMPA receptors. Ref.7
Miscellaneous	Widely used as activity-dependent neuronal marker to identify recently activated neurons in behavioral studies. Ref.7
Sequence similarities	Belongs to the ARC/ARG3.1 family.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Cell junction Cell membrane Cell projection Cytoplasm Cytoplasmic vesicle Cytoskeleton Endosome Membrane Postsynaptic cell membrane Synapse
Domain	Coiled coil
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	anterior/posterior pattern specification Inferred from mutant phenotype. Source: MGI cell migration Inferred from sequence or structural similarity. Source: UniProtKB cytoskeleton organization Inferred from sequence or structural similarity. Source: UniProtKB endocytosis Inferred from electronic annotation. Source: UniProtKB-KW endoderm development Inferred from mutant phenotype. Source: MGI regulation of cell morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	acrosomal vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell cell junction Inferred from electronic annotation. Source: UniProtKB-KW endosome Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW postsynaptic density Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	actin binding Traceable author statement. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Dlg4	Q62108	3	EBI-397779,EBI-300895

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	Activity-regulated cytoskeleton-associated protein		PRO_0000273286
Regions
	Region	89 – 100	12	Binds SH3GL1 or SH3GL3 By similarity UniProtKB Q63053
	Region	195 – 214	20	Required for binding DNM2 By similarity UniProtKB Q63053
	Coiled coil	54 – 78	25	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9WV31 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: F4D3505FD477D18A	FASTA	396	45,321
	10 20 30 40 50 60 MELDHMTTGG LHAYPAPRGG PAAKPNVILQ IGKCRAEMLE HVRRTHRHLL TEVSKQVERE 70 80 90 100 110 120 LKGLHRSVGK LENNLDGYVP TGDSQRWKKS IKACLCRCQE TIANLERWVK REMHVWREVF 130 140 150 160 170 180 YRLERWADRL ESMGGKYPVG SEPARHTVSV GVGGPEPYCQ EADGYDYTVS PYAITPPPAA 190 200 210 220 230 240 GELPEQESVE AQQYQSWGPG EDGQPSPGVD TQIFEDPREF LSHLEEYLRQ VGGSEEYWLS 250 260 270 280 290 300 QIQNHMNGPA KKWWEFKQGS VKNWVEFKKE FLQYSEGTLS REAIQRELEL PQKQGEPLDQ 310 320 330 340 350 360 FLWRKRDLYQ TLYVDAEEEE IIQYVVGTLQ PKLKRFLRHP LPKTLEQLIQ RGMEVQDGLE 370 380 390 QAAEPSGTPL PTEDETEALT PALTSESVAS DRTQPE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arg3.1/Arc mRNA induction by Ca2+ and cAMP requires protein kinase A and mitogen-activated protein kinase/extracellular regulated kinase activation." Waltereit R., Dammermann B., Wulff P., Scafidi J., Staubli U., Kauselmann G., Bundman M., Kuhl D. J. Neurosci. 21:5484-5493(2001) [PubMed: 11466419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv.
[2]	"The mArc gene, a mouse homolog of rat Arc." Chowdhury S., Lanahan A.A., Worley P.F. Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Forebrain.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Dendritic cell and Embryo.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary gland.
[5]	"Characterization of the promoter region of the immediate early gene Arc." Medrano S., Worley P.F., Chowdhury S., Lanahan A., Steward O., Scrable H. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-242. Strain: C57BL/6 X CBA.
[6]	"Developmental association of the synaptic activity-regulated protein arc with the mouse acrosomal organelle and the sperm tail." Maier B., Medrano S., Sleight S.B., Visconti P.E., Scrable H. Biol. Reprod. 68:67-76(2003) [PubMed: 12493697] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[7]	"Arc/Arg3.1 is essential for the consolidation of synaptic plasticity and memories." Plath N., Ohana O., Dammermann B., Errington M.L., Schmitz D., Gross C., Mao X., Engelsberg A., Mahlke C., Welzl H., Kobalz U., Stawrakakis A., Fernandez E., Waltereit R., Bick-Sander A., Therstappen E., Cooke S.F., Blanquet V. , Wurst W., Salmen B., Bosl M.R., Lipp H.P., Grant S.G., Bliss T.V., Wolfer D.P., Kuhl D. Neuron 52:437-444(2006) [PubMed: 17088210] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[8]	"Arc/Arg3.1 mediates homeostatic synaptic scaling of AMPA Receptors." Shepherd J.D., Rumbaugh G., Wu J., Chowdhury S., Plath N., Kuhl D., Huganir R.L., Worley P.F. Neuron 52:475-484(2006) [PubMed: 17088213] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF177701 Genomic DNA. Translation: AAK91587.1. AF162777 mRNA. Translation: AAD43586.1. AK157822 mRNA. Translation: BAE34212.1. AK170446 mRNA. Translation: BAE41804.1. BC023127 mRNA. Translation: AAH23127.1. AF254662 Genomic DNA. Translation: AAG10254.1.
IPI	IPI00125140.
RefSeq	NP_061260.1. NM_018790.2.
UniGene	Mm.25405.
3D structure databases
ProteinModelPortal	Q9WV31.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9WV31. 3 interactions.
STRING	Q9WV31.
Proteomic databases
PRIDE	Q9WV31.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000023268; ENSMUSP00000023268; ENSMUSG00000022602. ENSMUST00000110009; ENSMUSP00000105636; ENSMUSG00000022602.
GeneID	11838.
KEGG	mmu:11838.
UCSC	uc007wfn.1. mouse.
Organism-specific databases
CTD	23237.
MGI	MGI:88067. Arc.
Phylogenomic databases
eggNOG	maNOG11459.
GeneTree	ENSGT00390000003914.
HOGENOM	HBG269748.
HOVERGEN	HBG080862.
InParanoid	Q9WV31.
OMA	RCQETIA.
OrthoDB	EOG49S66B.
PhylomeDB	Q9WV31.
Gene expression databases
ArrayExpress	Q9WV31.
Bgee	Q9WV31.
Genevestigator	Q9WV31.
Family and domain databases
InterPro	IPR023263. Activity-reg_cytoskelet-assoc. [Graphical view]
PRINTS	PR02027. ARCARG31.
ProtoNet	Search...
Other
NextBio	279775.
SOURCE	Search...

Entry information

Entry name

ARC_MOUSE

Accession

Primary (citable) accession number: Q9WV31
Secondary accession number(s): Q9ES15

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	November 1, 1999
Last modified:	December 14, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program