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Q9WV30

- NFAT5_MOUSE

UniProt

Q9WV30 - NFAT5_MOUSE

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Protein

Nuclear factor of activated T-cells 5

Gene
Nfat5
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Transcription factor involved in the transcriptional regulation of osmoprotective and inflammatory genes. Regulates hypertonicity-induced cellular accumulation of osmolytes.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi293 – 3008 By similarity

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. sequence-specific DNA binding transcription factor activity Source: MGI

GO - Biological processi

  1. cytokine production Source: MGI
  2. positive regulation of gene expression Source: MGI
  3. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  4. regulation of calcineurin-NFAT signaling cascade Source: MGI
  5. transcription, DNA-templated Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear factor of activated T-cells 5
Short name:
NF-AT5
Alternative name(s):
Rel domain-containing transcription factor NFAT5
T-cell transcription factor NFAT5
Gene namesi
Name:Nfat5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:1859333. Nfat5.

Subcellular locationi

Nucleus. Cytoplasm
Note: Nuclear distribution increases under hypertonic conditions.1 Publication

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryonic and perinatal lethality with incomplete penetrance. At 17.5 dpc, 50% of the expected Mendelian rate with only 3.4% of the expected number living past 21 days after birth. The few survivors to adulthood fail to thrive and their weight is half compared to the wild-type. At 3 weeks old, kidney hypoplasia and an altered medullary morphology are observed with an increased apoptosis in the kidney inner medulla. Under osmotic stress, the transcriptional regulation of osmoprotective genes is altered in the kidney medulla.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15341534Nuclear factor of activated T-cells 5PRO_0000205184Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei120 – 1201Phosphoserine By similarity
Modified residuei122 – 1221N6-acetyllysine1 Publication
Modified residuei134 – 1341Phosphoserine By similarity
Modified residuei135 – 1351Phosphothreonine; by CDK5 By similarity
Modified residuei155 – 1551Phosphoserine By similarity

Post-translational modificationi

Phosphorylated at Thr-135 by CDK5 in response to osmotic stress; this phosphorylation mediates its rapid nuclear localization.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9WV30.
PaxDbiQ9WV30.
PRIDEiQ9WV30.

PTM databases

PhosphoSiteiQ9WV30.

Expressioni

Tissue specificityi

Detected in white and brown adipose tissue, muscle, heart, liver and kidney. Expressed in lymphocytes (at protein level).2 Publications

Developmental stagei

At 10.5 dpc, expressed in brain and developing lens. At 12.5 dpc, the expression is extended to liver. By 17.5 dpc, expressed abundantly in brain, spinal cord, heart end liver and moderately in salivary gland, lung, kidney, gut and bladder (at protein level).1 Publication

Inductioni

Up-regulated upon hypertonic conditions, this up-regulation in not observed in ES cells.2 Publications

Gene expression databases

ArrayExpressiQ9WV30.
BgeeiQ9WV30.
CleanExiMM_NFAT5.
GenevestigatoriQ9WV30.

Interactioni

Subunit structurei

Homodimer when bound to DNA, completely encircles its DNA target. Does not bind with Fos and Jun transcription factors. Interacts with CIDEC; this interaction is direct and retains NFAT5 in the cytoplasm By similarity.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000119370.

Structurei

3D structure databases

ProteinModelPortaliQ9WV30.
SMRiQ9WV30. Positions 264-544.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini264 – 443180RHDAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi69 – 10032Ser-richAdd
BLAST
Compositional biasi647 – 6537Poly-Ser
Compositional biasi738 – 7414Poly-Gln
Compositional biasi876 – 88510Poly-Gln
Compositional biasi963 – 9686Poly-Thr

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG85658.
GeneTreeiENSGT00550000074562.
HOGENOMiHOG000236245.
HOVERGENiHBG052612.
InParanoidiQ91WY0.
KOiK17335.
OMAiNNMTLAV.
OrthoDBiEOG79PJND.
TreeFamiTF326480.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.340. 1 hit.
InterProiIPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT.
IPR008366. NFAT.
IPR015646. NFAT5.
IPR008967. p53-like_TF_DNA-bd.
IPR011539. RHD.
[Graphical view]
PANTHERiPTHR12533. PTHR12533. 1 hit.
PTHR12533:SF7. PTHR12533:SF7. 1 hit.
PfamiPF00554. RHD. 1 hit.
[Graphical view]
PRINTSiPR01789. NUCFACTORATC.
SMARTiSM00429. IPT. 1 hit.
[Graphical view]
SUPFAMiSSF49417. SSF49417. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS50254. REL_2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Note: Additional isoforms seem to exist. Experimental confirmation may be lacking for some isoforms.

Isoform 1 (identifier: Q9WV30-1) [UniParc]FASTAAdd to Basket

Also known as: C

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MPSDFISLLS ADLDLESPKS LYSRESVYDL LPKELQLPPP RETSVASMSQ     50
TSGGEAGSPP PAVVAADASS APSSSSMGGA CSSFTTSSSP TIYSTSVTDS 100
KAMQVESCSS AVGVSNRGVS EKQLTGNTVQ QHPSTPKRHT VLYISPPPED 150
LLDNSRMSCQ DEGCGLESEQ SCSMWMEDSP SNFSNMSTSS YNDNTEVPRK 200
SRKRNPKQRP GVKRRDCEES NMDIFDADSA KAPHYVLSQL TTDNKGNSKA 250
GNGTLDSQKG TGVKKSPMLC GQYPVKSEGK ELKIVVQPET QHRARYLTEG 300
SRGSVKDRTQ QGFPTVKLEG HNEPVVLQVF VGNDSGRVKP HGFYQACRVT 350
GRNTTPCKEV DIEGTTVIEV GLDPSNNMTL AVDCVGILKL RNADVEARIG 400
IAGSKKKSTR ARLVFRVNIT RKDGSTLTLQ TPSSPILCTQ PAGVPEILKK 450
SLHSCSVKGE EEVFLIGKNF LKGTKVIFQE NVSDENSWKS EAEIDMELFH 500
QNHLIVKVPP YHDQHITLPV SVGIYVVTNA GRSHDVQPFT YTPDPAAGAL 550
NVNVKKEISS PARPCSFEEA MKAMKTTGCN VDKVTILPNA LITPLISSSM 600
IKTEDVTPME VTSEKRSSPI FQTTKSIGST QQTLETISNI AGGAPFSSPS 650
SSSHLTPESE NQQQLQPKAY NPETLTTIQT QDISQPGTFP AVSAASQLPS 700
SDALLQQATQ FQTREAQSRD TIQSDTVVNL SQLTEASQQQ QSPLQEQAQT 750
LQQQIPSNIF PSPSSVSQLQ STIQQLQAGS FTGSAAGGRS GSVDLVQQVL 800
EAQQQLSSVL FSTPDGNENV QEQLNADIFQ VSQIQNSVSP GMFSSAESAV 850
HTRPDNLLPG RADSVHQQTE NTLSNQQQQQ QQQQQVMESS AAMVMEMQQS 900
ICQAAAQIQS ELFPSAASAS GSLQQSPVYQ QPSHMMSALP TNEDMQMQCE 950
LFSSPPAASG NETSTTTTPQ VATPGSTMFQ TPSSGDGEET GAQAKQIQNS 1000
VFQTMVQMQR SGDSQPQVNL FSSTKNIMSV QNNGTQQQGN SLFQQGSEMM 1050
SLQSGNFLQQ SSHSQAQLFH PQNPIADAQN LSQETQGSIF HSPNPIVHSQ 1100
TSTASSEQLQ PSMFHSQNTI AVLQGSSVPQ DQQSPNIFLS QSSINNLQTN 1150
TVAQEEQISF FAAQNSISPL QSTSNTEQQA AFQQQPPISH IQTPILSQEQ 1200
AQPSQQGLFQ PQVALGSLPP NPMPQNQQGP IFQTQRPIVG MQSNSPSQEQ 1250
QQQQQQQQQQ QQQQQQQQQS ILFSNQNAMA TMASQKQPPP NMMFSPNQNP 1300
MASQEQQNQS IFHQQSNMAP MNQEQQPMQF QNQPTVSSLQ NPGPTQSESP 1350
QTSLFHSSPQ IQLVQGSPSS QDQQVTLFLS PASMSALQTS INQPDMQQSP 1400
LYSPQNNIPG IQGSTSSPQP QATLFHNTTG GTINQIQNSP GSSQQTSGMF 1450
LFGIQNNCSQ LLTSGPATLP DQLMAINQQG QPQNEGQSSV TTLLSQQMPE 1500
TSPLASSVNS SQNMEKIDLL VSLQSQGNNL TGSF 1534

Note: May have longer half-life and is more efficient in stimulation of transcription than isoform 3.

Length:1,534
Mass (Da):165,800
Last modified:October 16, 2013 - v2
Checksum:i996904A84D3A7AFE
GO
Isoform 2 (identifier: Q9WV30-2) [UniParc]FASTAAdd to Basket

Also known as: D

The sequence of this isoform differs from the canonical sequence as follows:
     24-24: R → RDSLKLHPSQTFHRAGLLE

Show »
Length:1,552
Mass (Da):167,832
Checksum:iE387B2F80B28E788
GO
Isoform 3 (identifier: Q9WV30-3) [UniParc]FASTAAdd to Basket

Also known as: A

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: Missing.

Show »
Length:1,458
Mass (Da):158,010
Checksum:i108F6CCD85985A12
GO
Isoform 4 (identifier: Q9WV30-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1213-1225: VALGSLPPNPMPQ → ESLHSHITPDACK
     1226-1534: Missing.

Show »
Length:1,225
Mass (Da):132,192
Checksum:i6A91537E7949A527
GO

Sequence cautioni

The sequence AAF31405.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
The sequence EDL11405.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7676Missing in isoform 3. VSP_053211Add
BLAST
Alternative sequencei24 – 241R → RDSLKLHPSQTFHRAGLLE in isoform 2. VSP_053212
Alternative sequencei1213 – 122513VALGS…NPMPQ → ESLHSHITPDACK in isoform 4. VSP_053213Add
BLAST
Alternative sequencei1226 – 1534309Missing in isoform 4. VSP_053214Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti108 – 1081C → G in AAK97603. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF162853 mRNA. Translation: AAD44343.1.
AF369980 mRNA. Translation: AAK97603.1.
AC125207 Genomic DNA. No translation available.
AC158364 Genomic DNA. No translation available.
CH466525 Genomic DNA. Translation: EDL11404.1.
CH466525 Genomic DNA. Translation: EDL11405.1. Sequence problems.
AF200687 mRNA. Translation: AAF31405.1. Different initiation.
CCDSiCCDS22648.2. [Q9WV30-2]
CCDS40464.1. [Q9WV30-3]
CCDS72168.1. [Q9WV30-4]
RefSeqiNP_001273189.1. NM_001286260.1. [Q9WV30-4]
NP_061293.2. NM_018823.2. [Q9WV30-2]
NP_598718.2. NM_133957.3. [Q9WV30-3]
XP_006531255.1. XM_006531192.1. [Q9WV30-1]
UniGeneiMm.390057.

Genome annotation databases

EnsembliENSMUST00000075922; ENSMUSP00000075311; ENSMUSG00000003847. [Q9WV30-4]
ENSMUST00000077440; ENSMUSP00000076653; ENSMUSG00000003847. [Q9WV30-3]
ENSMUST00000125721; ENSMUSP00000116094; ENSMUSG00000003847. [Q9WV30-1]
ENSMUST00000151114; ENSMUSP00000119370; ENSMUSG00000003847. [Q9WV30-2]
ENSMUST00000169453; ENSMUSP00000127784; ENSMUSG00000003847. [Q9WV30-2]
GeneIDi54446.
KEGGimmu:54446.
UCSCiuc009nhl.2. mouse.
uc009nhm.2. mouse. [Q9WV30-1]
uc012gkd.1. mouse.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF162853 mRNA. Translation: AAD44343.1 .
AF369980 mRNA. Translation: AAK97603.1 .
AC125207 Genomic DNA. No translation available.
AC158364 Genomic DNA. No translation available.
CH466525 Genomic DNA. Translation: EDL11404.1 .
CH466525 Genomic DNA. Translation: EDL11405.1 . Sequence problems.
AF200687 mRNA. Translation: AAF31405.1 . Different initiation.
CCDSi CCDS22648.2. [Q9WV30-2 ]
CCDS40464.1. [Q9WV30-3 ]
CCDS72168.1. [Q9WV30-4 ]
RefSeqi NP_001273189.1. NM_001286260.1. [Q9WV30-4 ]
NP_061293.2. NM_018823.2. [Q9WV30-2 ]
NP_598718.2. NM_133957.3. [Q9WV30-3 ]
XP_006531255.1. XM_006531192.1. [Q9WV30-1 ]
UniGenei Mm.390057.

3D structure databases

ProteinModelPortali Q9WV30.
SMRi Q9WV30. Positions 264-544.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000119370.

PTM databases

PhosphoSitei Q9WV30.

Proteomic databases

MaxQBi Q9WV30.
PaxDbi Q9WV30.
PRIDEi Q9WV30.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000075922 ; ENSMUSP00000075311 ; ENSMUSG00000003847 . [Q9WV30-4 ]
ENSMUST00000077440 ; ENSMUSP00000076653 ; ENSMUSG00000003847 . [Q9WV30-3 ]
ENSMUST00000125721 ; ENSMUSP00000116094 ; ENSMUSG00000003847 . [Q9WV30-1 ]
ENSMUST00000151114 ; ENSMUSP00000119370 ; ENSMUSG00000003847 . [Q9WV30-2 ]
ENSMUST00000169453 ; ENSMUSP00000127784 ; ENSMUSG00000003847 . [Q9WV30-2 ]
GeneIDi 54446.
KEGGi mmu:54446.
UCSCi uc009nhl.2. mouse.
uc009nhm.2. mouse. [Q9WV30-1 ]
uc012gkd.1. mouse.

Organism-specific databases

CTDi 10725.
MGIi MGI:1859333. Nfat5.

Phylogenomic databases

eggNOGi NOG85658.
GeneTreei ENSGT00550000074562.
HOGENOMi HOG000236245.
HOVERGENi HBG052612.
InParanoidi Q91WY0.
KOi K17335.
OMAi NNMTLAV.
OrthoDBi EOG79PJND.
TreeFami TF326480.

Miscellaneous databases

ChiTaRSi NFAT5. mouse.
NextBioi 311314.
PROi Q9WV30.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9WV30.
Bgeei Q9WV30.
CleanExi MM_NFAT5.
Genevestigatori Q9WV30.

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
2.60.40.340. 1 hit.
InterProi IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT.
IPR008366. NFAT.
IPR015646. NFAT5.
IPR008967. p53-like_TF_DNA-bd.
IPR011539. RHD.
[Graphical view ]
PANTHERi PTHR12533. PTHR12533. 1 hit.
PTHR12533:SF7. PTHR12533:SF7. 1 hit.
Pfami PF00554. RHD. 1 hit.
[Graphical view ]
PRINTSi PR01789. NUCFACTORATC.
SMARTi SM00429. IPT. 1 hit.
[Graphical view ]
SUPFAMi SSF49417. SSF49417. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEi PS50254. REL_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of NFAT5, a new rel-like transcription factor."
    Lu J., Xu H., Olson E.N.
    Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4).
  2. "Mouse TonEBP-NFAT5: expression in early development and alternative splicing."
    Maouyo D., Kim J.Y., Lee S.D., Wu Y., Woo S.K., Kwon H.M.
    Am. J. Physiol. 282:F802-F809(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), ALTERNATIVE SPLICING, FUNCTION, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, INDUCTION.
    Tissue: Brain.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-1534 (ISOFORM 2).
    Tissue: Skeletal muscle.
  6. Cited for: FUNCTION IN RENAL HOMEOSTASIS, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
  7. "Fat-specific protein 27 modulates nuclear factor of activated T cells 5 and the cellular response to stress."
    Ueno M., Shen W.J., Patel S., Greenberg A.S., Azhar S., Kraemer F.B.
    J. Lipid Res. 54:734-743(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION BY OSMOTIC STRESS.
  8. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-122, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiNFAT5_MOUSE
AccessioniPrimary (citable) accession number: Q9WV30
Secondary accession number(s): E9Q0P3
, E9Q6U2, G5E8N9, Q91WY0, Q9JLA1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 16, 2013
Last modified: July 9, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi