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Q9WV27

- AT1A4_MOUSE

UniProt

Q9WV27 - AT1A4_MOUSE

Protein

Sodium/potassium-transporting ATPase subunit alpha-4

Gene

Atp1a4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Plays a role in sperm motility By similarity.By similarity

    Catalytic activityi

    ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

    Enzyme regulationi

    Specifically inhibited by an endogenous cardiac glycoside, ouabain.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei386 – 38614-aspartylphosphate intermediateBy similarity
    Metal bindingi726 – 7261MagnesiumBy similarity
    Metal bindingi730 – 7301MagnesiumBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. sodium:potassium-exchanging ATPase activity Source: MGI

    GO - Biological processi

    1. ATP biosynthetic process Source: InterPro
    2. fertilization Source: MGI
    3. regulation of cellular pH Source: UniProtKB
    4. regulation of membrane potential Source: MGI
    5. sodium ion transmembrane transport Source: GOC
    6. sodium ion transport Source: MGI
    7. spermatogenesis Source: MGI
    8. sperm motility Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium/potassium-transporting ATPase subunit alpha-4 (EC:3.6.3.9)
    Short name:
    Na(+)/K(+) ATPase alpha-4 subunit
    Alternative name(s):
    Sodium pump subunit alpha-4
    Gene namesi
    Name:Atp1a4
    Synonyms:Atp1al2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:1351335. Atp1a4.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10321032Sodium/potassium-transporting ATPase subunit alpha-4PRO_0000046304Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei952 – 9521Phosphoserine; by PKABy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9WV27.
    PaxDbiQ9WV27.
    PRIDEiQ9WV27.

    PTM databases

    PhosphoSiteiQ9WV27.

    Expressioni

    Tissue specificityi

    Expressed at high levels in the testis and at low levels in the epididymis.

    Gene expression databases

    BgeeiQ9WV27.
    GenevestigatoriQ9WV27.

    Interactioni

    Subunit structurei

    Composed of three subunits: alpha (catalytic), beta and gamma.

    Protein-protein interaction databases

    IntActiQ9WV27. 4 interactions.
    MINTiMINT-4088554.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9WV27.
    SMRiQ9WV27. Positions 39-1032.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 9696CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini118 – 14124ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini163 – 298136CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini319 – 33012ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini349 – 781433CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini802 – 81110ExtracellularSequence Analysis
    Topological domaini833 – 85220CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini876 – 92752ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini948 – 96013CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini980 – 99415ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1016 – 103217CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei97 – 11721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei142 – 16221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei299 – 31820HelicalSequence AnalysisAdd
    BLAST
    Transmembranei331 – 34818HelicalSequence AnalysisAdd
    BLAST
    Transmembranei782 – 80120HelicalSequence AnalysisAdd
    BLAST
    Transmembranei812 – 83221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei853 – 87523HelicalSequence AnalysisAdd
    BLAST
    Transmembranei928 – 94720HelicalSequence AnalysisAdd
    BLAST
    Transmembranei961 – 97919HelicalSequence AnalysisAdd
    BLAST
    Transmembranei995 – 101521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni91 – 933Interaction with phosphoinositide-3 kinaseBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0474.
    GeneTreeiENSGT00560000076866.
    HOGENOMiHOG000265622.
    HOVERGENiHBG004298.
    InParanoidiQ9WV27.
    KOiK01539.
    OMAiKSSDTWF.
    OrthoDBiEOG7327N0.
    TreeFamiTF312838.

    Family and domain databases

    Gene3Di1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR005775. ATPase_P-typ_Na/K_IIC.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view]
    PfamiPF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view]
    PRINTSiPR00119. CATATPASE.
    SMARTiSM00831. Cation_ATPase_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 2 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9WV27-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEPGKEKEVE APGELNQKPR PSTRSSTTNR QPKMKRRKKD LEELKKEVVM     50
    DDHKLTLDEL SAKYSVDLTK GLSILEAQDI LFQNGPNVLT PPPTTPEWVK 100
    FCRQLFGGFS LLLWTGACLC FLAYGIHVNY YKENANKDNL YLGIVLSAVV 150
    IITGCFSYYQ EAKSSKIMES FKNMVPQQAL VIRDGEKMQI NVRDVVLGDL 200
    VEVKGGDQIP ADIRVISAQG CKVDNSSLTG ESEPQSRCPD CTHENPLETR 250
    NIIFFSTNCV EGTARGIVIA TGDYTVMGRI ASLTSGLQMG KTPIATEIEH 300
    FIHLITAVAV FLGVSFFWLS IILGYTWLDA VIFLIGIIVA NVPEGLLATV 350
    TVCLTLTAKR MARKNCLVKN LEAVETLGST STICSDKTGT LTQNRMTVAH 400
    LWFDKTVYEA DTSEEQTTGK TFPKSSDTWF YLARIAGLCN RADFKPHQES 450
    VPIAKRATTG DASESALLKF IEQSYNPVSE MRQKNPKVAE IPFNSTNKYQ 500
    MSIHLLEDNS EAHVLLMKGA PERIFDFCSS FLLNGQEYPM DEEMKMDFQN 550
    AYIELGGLGE RVLGFCFLNL PSNFSKGFQF NTDELNFPME NLCFAGLISM 600
    IDPPRTAVPD AVSKCRSAGI KVIMVTGDHP ITAKAIAKSV GIISEGNDTA 650
    EDIAARLNIP ISQVNNKSVK AIVVHGSELK DMESQQLDDI LKSYKEIVFA 700
    RTSPQQKLII VEGCQRLGAI VAVTGDGVND SPALKKADIG IAMGITGSDV 750
    SKQAADMILL DDNFASIVTG VEEGRLIFDN LKKSIAYTLT SNIPEITPFL 800
    LFIILSIPLP LGTITILCID LGTDMVPAIS LAYESPESDI MKRLPRNPKT 850
    DNLVNNRLIG MAYGQIGMIQ ALAGFFTYFV ILAENGFKPL DLLGIRLYWD 900
    DTQLNDLEDS YGQQWTYEQR KVVEFTCQTA FFISIVIVQW ADLIICKTRR 950
    NSLFKQGMKN KILIFGLLEE TVLAAFLSYV PGMDVSLRMY PLKINWWFCA 1000
    LPYSVLIFVY DEIRKLIIRR RPGGWLEKET YY 1032
    Length:1,032
    Mass (Da):114,887
    Last modified:July 27, 2011 - v3
    Checksum:i5B97565BA67AA08C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti74 – 741I → V in AAD43812. (PubMed:10555956)Curated
    Sequence conflicti476 – 4761N → S in AAD43813. (PubMed:10555956)Curated
    Sequence conflicti546 – 5461M → T in AAD43813. (PubMed:10555956)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF164348 Genomic DNA. Translation: AAD43812.1.
    AF164349 Genomic DNA. Translation: AAD43813.1.
    AF164350 Genomic DNA. Translation: AAD43814.1.
    AC074310 Genomic DNA. No translation available.
    AC087061 Genomic DNA. No translation available.
    CCDSiCCDS48446.1.
    RefSeqiNP_038762.1. NM_013734.1.
    UniGeneiMm.482392.

    Genome annotation databases

    EnsembliENSMUST00000111243; ENSMUSP00000106874; ENSMUSG00000007107.
    GeneIDi27222.
    KEGGimmu:27222.
    UCSCiuc011wwk.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF164348 Genomic DNA. Translation: AAD43812.1 .
    AF164349 Genomic DNA. Translation: AAD43813.1 .
    AF164350 Genomic DNA. Translation: AAD43814.1 .
    AC074310 Genomic DNA. No translation available.
    AC087061 Genomic DNA. No translation available.
    CCDSi CCDS48446.1.
    RefSeqi NP_038762.1. NM_013734.1.
    UniGenei Mm.482392.

    3D structure databases

    ProteinModelPortali Q9WV27.
    SMRi Q9WV27. Positions 39-1032.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9WV27. 4 interactions.
    MINTi MINT-4088554.

    PTM databases

    PhosphoSitei Q9WV27.

    Proteomic databases

    MaxQBi Q9WV27.
    PaxDbi Q9WV27.
    PRIDEi Q9WV27.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000111243 ; ENSMUSP00000106874 ; ENSMUSG00000007107 .
    GeneIDi 27222.
    KEGGi mmu:27222.
    UCSCi uc011wwk.1. mouse.

    Organism-specific databases

    CTDi 480.
    MGIi MGI:1351335. Atp1a4.

    Phylogenomic databases

    eggNOGi COG0474.
    GeneTreei ENSGT00560000076866.
    HOGENOMi HOG000265622.
    HOVERGENi HBG004298.
    InParanoidi Q9WV27.
    KOi K01539.
    OMAi KSSDTWF.
    OrthoDBi EOG7327N0.
    TreeFami TF312838.

    Miscellaneous databases

    NextBioi 305134.
    PROi Q9WV27.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9WV27.
    Genevestigatori Q9WV27.

    Family and domain databases

    Gene3Di 1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProi IPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR005775. ATPase_P-typ_Na/K_IIC.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view ]
    Pfami PF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view ]
    PRINTSi PR00119. CATATPASE.
    SMARTi SM00831. Cation_ATPase_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 2 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsi TIGR01106. ATPase-IIC_X-K. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Na,K-ATPase alpha4 gene (Atp1a4) encodes a ouabain-resistant alpha subunit and is tightly linked to the alpha2 gene (Atp1a2) on mouse chromosome 1."
      Underhill D.A., Canfield V.A., Dahl J.P., Gros P., Levenson R.
      Biochemistry 38:14746-14751(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: C57BL/6J and CD-1.
      Tissue: Mammary gland and Testis.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. Lubec G., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 173-183; 370-387; 488-498; 606-634; 708-716 AND 736-783, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain.

    Entry informationi

    Entry nameiAT1A4_MOUSE
    AccessioniPrimary (citable) accession number: Q9WV27
    Secondary accession number(s): E9QKL5, Q9R173, Q9WV28
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 18, 2001
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 123 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3