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Protein

Sodium/potassium-transporting ATPase subunit alpha-4

Gene

Atp1a4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Plays a role in sperm motility (By similarity).By similarity

Catalytic activityi

ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

Enzyme regulationi

Specifically inhibited by an endogenous cardiac glycoside, ouabain.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei386 – 38614-aspartylphosphate intermediateBy similarity
Metal bindingi726 – 7261MagnesiumBy similarity
Metal bindingi730 – 7301MagnesiumBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. sodium:potassium-exchanging ATPase activity Source: MGI

GO - Biological processi

  1. fertilization Source: MGI
  2. regulation of cellular pH Source: UniProtKB
  3. regulation of membrane potential Source: MGI
  4. sodium ion transmembrane transport Source: GOC
  5. sodium ion transport Source: MGI
  6. spermatogenesis Source: MGI
  7. sperm motility Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium

Enzyme and pathway databases

ReactomeiREACT_338561. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/potassium-transporting ATPase subunit alpha-4 (EC:3.6.3.9)
Short name:
Na(+)/K(+) ATPase alpha-4 subunit
Alternative name(s):
Sodium pump subunit alpha-4
Gene namesi
Name:Atp1a4
Synonyms:Atp1al2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1351335. Atp1a4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9696CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei97 – 11721HelicalSequence AnalysisAdd
BLAST
Topological domaini118 – 14124ExtracellularSequence AnalysisAdd
BLAST
Transmembranei142 – 16221HelicalSequence AnalysisAdd
BLAST
Topological domaini163 – 298136CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei299 – 31820HelicalSequence AnalysisAdd
BLAST
Topological domaini319 – 33012ExtracellularSequence AnalysisAdd
BLAST
Transmembranei331 – 34818HelicalSequence AnalysisAdd
BLAST
Topological domaini349 – 781433CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei782 – 80120HelicalSequence AnalysisAdd
BLAST
Topological domaini802 – 81110ExtracellularSequence Analysis
Transmembranei812 – 83221HelicalSequence AnalysisAdd
BLAST
Topological domaini833 – 85220CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei853 – 87523HelicalSequence AnalysisAdd
BLAST
Topological domaini876 – 92752ExtracellularSequence AnalysisAdd
BLAST
Transmembranei928 – 94720HelicalSequence AnalysisAdd
BLAST
Topological domaini948 – 96013CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei961 – 97919HelicalSequence AnalysisAdd
BLAST
Topological domaini980 – 99415ExtracellularSequence AnalysisAdd
BLAST
Transmembranei995 – 101521HelicalSequence AnalysisAdd
BLAST
Topological domaini1016 – 103217CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10321032Sodium/potassium-transporting ATPase subunit alpha-4PRO_0000046304Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei952 – 9521Phosphoserine; by PKABy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9WV27.
PaxDbiQ9WV27.
PRIDEiQ9WV27.

PTM databases

PhosphoSiteiQ9WV27.

Expressioni

Tissue specificityi

Expressed at high levels in the testis and at low levels in the epididymis.

Gene expression databases

BgeeiQ9WV27.
ExpressionAtlasiQ9WV27. baseline and differential.
GenevestigatoriQ9WV27.

Interactioni

Subunit structurei

Composed of three subunits: alpha (catalytic), beta and gamma.

Protein-protein interaction databases

IntActiQ9WV27. 4 interactions.
MINTiMINT-4088554.

Structurei

3D structure databases

ProteinModelPortaliQ9WV27.
SMRiQ9WV27. Positions 39-1032.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni91 – 933Interaction with phosphoinositide-3 kinaseBy similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
GeneTreeiENSGT00760000119003.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
InParanoidiQ9WV27.
KOiK01539.
OMAiKSSDTWF.
OrthoDBiEOG7327N0.
TreeFamiTF312838.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WV27-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPGKEKEVE APGELNQKPR PSTRSSTTNR QPKMKRRKKD LEELKKEVVM
60 70 80 90 100
DDHKLTLDEL SAKYSVDLTK GLSILEAQDI LFQNGPNVLT PPPTTPEWVK
110 120 130 140 150
FCRQLFGGFS LLLWTGACLC FLAYGIHVNY YKENANKDNL YLGIVLSAVV
160 170 180 190 200
IITGCFSYYQ EAKSSKIMES FKNMVPQQAL VIRDGEKMQI NVRDVVLGDL
210 220 230 240 250
VEVKGGDQIP ADIRVISAQG CKVDNSSLTG ESEPQSRCPD CTHENPLETR
260 270 280 290 300
NIIFFSTNCV EGTARGIVIA TGDYTVMGRI ASLTSGLQMG KTPIATEIEH
310 320 330 340 350
FIHLITAVAV FLGVSFFWLS IILGYTWLDA VIFLIGIIVA NVPEGLLATV
360 370 380 390 400
TVCLTLTAKR MARKNCLVKN LEAVETLGST STICSDKTGT LTQNRMTVAH
410 420 430 440 450
LWFDKTVYEA DTSEEQTTGK TFPKSSDTWF YLARIAGLCN RADFKPHQES
460 470 480 490 500
VPIAKRATTG DASESALLKF IEQSYNPVSE MRQKNPKVAE IPFNSTNKYQ
510 520 530 540 550
MSIHLLEDNS EAHVLLMKGA PERIFDFCSS FLLNGQEYPM DEEMKMDFQN
560 570 580 590 600
AYIELGGLGE RVLGFCFLNL PSNFSKGFQF NTDELNFPME NLCFAGLISM
610 620 630 640 650
IDPPRTAVPD AVSKCRSAGI KVIMVTGDHP ITAKAIAKSV GIISEGNDTA
660 670 680 690 700
EDIAARLNIP ISQVNNKSVK AIVVHGSELK DMESQQLDDI LKSYKEIVFA
710 720 730 740 750
RTSPQQKLII VEGCQRLGAI VAVTGDGVND SPALKKADIG IAMGITGSDV
760 770 780 790 800
SKQAADMILL DDNFASIVTG VEEGRLIFDN LKKSIAYTLT SNIPEITPFL
810 820 830 840 850
LFIILSIPLP LGTITILCID LGTDMVPAIS LAYESPESDI MKRLPRNPKT
860 870 880 890 900
DNLVNNRLIG MAYGQIGMIQ ALAGFFTYFV ILAENGFKPL DLLGIRLYWD
910 920 930 940 950
DTQLNDLEDS YGQQWTYEQR KVVEFTCQTA FFISIVIVQW ADLIICKTRR
960 970 980 990 1000
NSLFKQGMKN KILIFGLLEE TVLAAFLSYV PGMDVSLRMY PLKINWWFCA
1010 1020 1030
LPYSVLIFVY DEIRKLIIRR RPGGWLEKET YY
Length:1,032
Mass (Da):114,887
Last modified:July 27, 2011 - v3
Checksum:i5B97565BA67AA08C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti74 – 741I → V in AAD43812 (PubMed:10555956).Curated
Sequence conflicti476 – 4761N → S in AAD43813 (PubMed:10555956).Curated
Sequence conflicti546 – 5461M → T in AAD43813 (PubMed:10555956).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF164348 Genomic DNA. Translation: AAD43812.1.
AF164349 Genomic DNA. Translation: AAD43813.1.
AF164350 Genomic DNA. Translation: AAD43814.1.
AC074310 Genomic DNA. No translation available.
AC087061 Genomic DNA. No translation available.
CCDSiCCDS48446.1.
RefSeqiNP_038762.1. NM_013734.1.
UniGeneiMm.482392.

Genome annotation databases

EnsembliENSMUST00000111243; ENSMUSP00000106874; ENSMUSG00000007107.
GeneIDi27222.
KEGGimmu:27222.
UCSCiuc011wwk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF164348 Genomic DNA. Translation: AAD43812.1.
AF164349 Genomic DNA. Translation: AAD43813.1.
AF164350 Genomic DNA. Translation: AAD43814.1.
AC074310 Genomic DNA. No translation available.
AC087061 Genomic DNA. No translation available.
CCDSiCCDS48446.1.
RefSeqiNP_038762.1. NM_013734.1.
UniGeneiMm.482392.

3D structure databases

ProteinModelPortaliQ9WV27.
SMRiQ9WV27. Positions 39-1032.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9WV27. 4 interactions.
MINTiMINT-4088554.

PTM databases

PhosphoSiteiQ9WV27.

Proteomic databases

MaxQBiQ9WV27.
PaxDbiQ9WV27.
PRIDEiQ9WV27.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000111243; ENSMUSP00000106874; ENSMUSG00000007107.
GeneIDi27222.
KEGGimmu:27222.
UCSCiuc011wwk.1. mouse.

Organism-specific databases

CTDi480.
MGIiMGI:1351335. Atp1a4.

Phylogenomic databases

eggNOGiCOG0474.
GeneTreeiENSGT00760000119003.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
InParanoidiQ9WV27.
KOiK01539.
OMAiKSSDTWF.
OrthoDBiEOG7327N0.
TreeFamiTF312838.

Enzyme and pathway databases

ReactomeiREACT_338561. Ion transport by P-type ATPases.

Miscellaneous databases

NextBioi305134.
PROiQ9WV27.
SOURCEiSearch...

Gene expression databases

BgeeiQ9WV27.
ExpressionAtlasiQ9WV27. baseline and differential.
GenevestigatoriQ9WV27.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Na,K-ATPase alpha4 gene (Atp1a4) encodes a ouabain-resistant alpha subunit and is tightly linked to the alpha2 gene (Atp1a2) on mouse chromosome 1."
    Underhill D.A., Canfield V.A., Dahl J.P., Gros P., Levenson R.
    Biochemistry 38:14746-14751(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: C57BL/6J and CD-1.
    Tissue: Mammary gland and Testis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 173-183; 370-387; 488-498; 606-634; 708-716 AND 736-783, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiAT1A4_MOUSE
AccessioniPrimary (citable) accession number: Q9WV27
Secondary accession number(s): E9QKL5, Q9R173, Q9WV28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: July 27, 2011
Last modified: April 1, 2015
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.