Sodium/potassium-transporting ATPase subunit alpha-4

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Q9WV27 (AT1A4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 112. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Sodium/potassium-transporting ATPase subunit alpha-4
Short name=Na(+)/K(+) ATPase alpha-4 subunit
EC=3.6.3.9

Alternative name(s):
Sodium pump subunit alpha-4

Gene names

Name:	Atp1a4
Synonyms:	Atp1al2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1032 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Plays a role in sperm motility By similarity.
Catalytic activity	ATP + H₂O + Na⁺(In) + K⁺(Out) = ADP + phosphate + Na⁺(Out) + K⁺(In).
Enzyme regulation	Specifically inhibited by an endogenous cardiac glycoside, ouabain By similarity.
Subunit structure	Composed of three subunits: alpha (catalytic), beta and gamma.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Expressed at high levels in the testis and at low levels in the epididymis.
Sequence similarities	Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Sodium transport Sodium/potassium transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Potassium Sodium
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro fertilization Inferred from mutant phenotype PubMed 21187400. Source: MGI regulation of cellular pH Inferred from sequence or structural similarity. Source: UniProtKB regulation of membrane potential Inferred from mutant phenotype PubMed 21187400. Source: MGI sperm motility Inferred from sequence or structural similarity. Source: UniProtKB spermatogenesis Inferred from mutant phenotype PubMed 21187400. Source: MGI
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW sodium:potassium-exchanging ATPase activity Inferred from mutant phenotype PubMed 21187400. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1032

1032

Sodium/potassium-transporting ATPase subunit alpha-4

PRO_0000046304

Regions

Topological domain

1 – 96

Cytoplasmic Potential

Transmembrane

97 – 117

Helical; Potential

Topological domain

118 – 141

Extracellular Potential

Transmembrane

142 – 162

Helical; Potential

Topological domain

163 – 298

136

Cytoplasmic Potential

Transmembrane

299 – 318

Helical; Potential

Topological domain

319 – 330

Extracellular Potential

Transmembrane

331 – 348

Helical; Potential

Topological domain

349 – 781

433

Cytoplasmic Potential

Transmembrane

782 – 801

Helical; Potential

Topological domain

802 – 811

Extracellular Potential

Transmembrane

812 – 832

Helical; Potential

Topological domain

833 – 852

Cytoplasmic Potential

Transmembrane

853 – 875

Helical; Potential

Topological domain

876 – 927

Extracellular Potential

Transmembrane

928 – 947

Helical; Potential

Topological domain

948 – 960

Cytoplasmic Potential

Transmembrane

961 – 979

Helical; Potential

Topological domain

980 – 994

Extracellular Potential

Transmembrane

995 – 1015

Helical; Potential

Topological domain

1016 – 1032

Cytoplasmic Potential

Region

91 – 93

Interaction with phosphoinositide-3 kinase By similarity

Sites

Active site

386

4-aspartylphosphate intermediate By similarity

Metal binding

726

Magnesium By similarity

Metal binding

730

Magnesium By similarity

Amino acid modifications

Modified residue

952

Phosphoserine; by PKA By similarity

Experimental info

Sequence conflict

I → V in AAD43812. Ref.1

Sequence conflict

476

N → S in AAD43813. Ref.1

Sequence conflict

546

M → T in AAD43813. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q9WV27 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 5B97565BA67AA08C
Show »

FASTA

1,032

114,887

        10         20         30         40         50         60 
MEPGKEKEVE APGELNQKPR PSTRSSTTNR QPKMKRRKKD LEELKKEVVM DDHKLTLDEL 

        70         80         90        100        110        120 
SAKYSVDLTK GLSILEAQDI LFQNGPNVLT PPPTTPEWVK FCRQLFGGFS LLLWTGACLC 

       130        140        150        160        170        180 
FLAYGIHVNY YKENANKDNL YLGIVLSAVV IITGCFSYYQ EAKSSKIMES FKNMVPQQAL 

       190        200        210        220        230        240 
VIRDGEKMQI NVRDVVLGDL VEVKGGDQIP ADIRVISAQG CKVDNSSLTG ESEPQSRCPD 

       250        260        270        280        290        300 
CTHENPLETR NIIFFSTNCV EGTARGIVIA TGDYTVMGRI ASLTSGLQMG KTPIATEIEH 

       310        320        330        340        350        360 
FIHLITAVAV FLGVSFFWLS IILGYTWLDA VIFLIGIIVA NVPEGLLATV TVCLTLTAKR 

       370        380        390        400        410        420 
MARKNCLVKN LEAVETLGST STICSDKTGT LTQNRMTVAH LWFDKTVYEA DTSEEQTTGK 

       430        440        450        460        470        480 
TFPKSSDTWF YLARIAGLCN RADFKPHQES VPIAKRATTG DASESALLKF IEQSYNPVSE 

       490        500        510        520        530        540 
MRQKNPKVAE IPFNSTNKYQ MSIHLLEDNS EAHVLLMKGA PERIFDFCSS FLLNGQEYPM 

       550        560        570        580        590        600 
DEEMKMDFQN AYIELGGLGE RVLGFCFLNL PSNFSKGFQF NTDELNFPME NLCFAGLISM 

       610        620        630        640        650        660 
IDPPRTAVPD AVSKCRSAGI KVIMVTGDHP ITAKAIAKSV GIISEGNDTA EDIAARLNIP 

       670        680        690        700        710        720 
ISQVNNKSVK AIVVHGSELK DMESQQLDDI LKSYKEIVFA RTSPQQKLII VEGCQRLGAI 

       730        740        750        760        770        780 
VAVTGDGVND SPALKKADIG IAMGITGSDV SKQAADMILL DDNFASIVTG VEEGRLIFDN 

       790        800        810        820        830        840 
LKKSIAYTLT SNIPEITPFL LFIILSIPLP LGTITILCID LGTDMVPAIS LAYESPESDI 

       850        860        870        880        890        900 
MKRLPRNPKT DNLVNNRLIG MAYGQIGMIQ ALAGFFTYFV ILAENGFKPL DLLGIRLYWD 

       910        920        930        940        950        960 
DTQLNDLEDS YGQQWTYEQR KVVEFTCQTA FFISIVIVQW ADLIICKTRR NSLFKQGMKN 

       970        980        990       1000       1010       1020 
KILIFGLLEE TVLAAFLSYV PGMDVSLRMY PLKINWWFCA LPYSVLIFVY DEIRKLIIRR 

      1030 
RPGGWLEKET YY

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Na,K-ATPase alpha4 gene (Atp1a4) encodes a ouabain-resistant alpha subunit and is tightly linked to the alpha2 gene (Atp1a2) on mouse chromosome 1." Underhill D.A., Canfield V.A., Dahl J.P., Gros P., Levenson R. Biochemistry 38:14746-14751(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/6J and CD-1. Tissue: Mammary gland and Testis.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 173-183; 370-387; 488-498; 606-634; 708-716 AND 736-783, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF164348 Genomic DNA. Translation: AAD43812.1. AF164349 Genomic DNA. Translation: AAD43813.1. AF164350 Genomic DNA. Translation: AAD43814.1. AC074310 Genomic DNA. No translation available. AC087061 Genomic DNA. No translation available.
IPI	IPI00378485.
RefSeq	NP_038762.1. NM_013734.1.
UniGene	Mm.482392.
3D structure databases
ProteinModelPortal	Q9WV27.
SMR	Q9WV27. Positions 36-1032.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9WV27. 1 interaction.
PTM databases
PhosphoSite	Q9WV27.
Proteomic databases
PaxDb	Q9WV27.
PRIDE	Q9WV27.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000111243; ENSMUSP00000106874; ENSMUSG00000007107.
GeneID	27222.
KEGG	mmu:27222.
UCSC	uc011wwk.1. mouse.
Organism-specific databases
CTD	480.
MGI	MGI:1351335. Atp1a4.
Phylogenomic databases
eggNOG	COG0474.
GeneTree	ENSGT00560000076866.
HOGENOM	HOG000265622.
HOVERGEN	HBG004298.
InParanoid	Q9WV27.
KO	K01539.
OMA	SHQRAKE.
OrthoDB	EOG4WDDB0.
Gene expression databases
ArrayExpress	Q9WV27.
Bgee	Q9WV27.
Genevestigator	Q9WV27.
GermOnline	ENSMUSG00000007107. Mus musculus.
Family and domain databases
Gene3D	1.20.1110.10. 2 hits. 2.70.150.10. 2 hits. 3.40.1110.10. 1 hit.
InterPro	IPR006068. ATPase_P-typ_cation-transptr_C. IPR004014. ATPase_P-typ_cation-transptr_N. IPR023299. ATPase_P-typ_cyto_domN. IPR005775. ATPase_P-typ_Na/K_IIC. IPR018303. ATPase_P-typ_P_site. IPR023298. ATPase_P-typ_TM_dom. IPR008250. ATPase_P-typ_transduc_dom_A. IPR001757. Cation_transp_P_typ_ATPase. IPR023214. HAD-like_dom. [Graphical view]
PANTHER	PTHR24093. PTHR24093. 1 hit.
Pfam	PF00689. Cation_ATPase_C. 1 hit. PF00690. Cation_ATPase_N. 1 hit. PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE.
SMART	SM00831. Cation_ATPase_N. 1 hit. [Graphical view]
SUPFAM	SSF81660. ATPase_cation_domN. 1 hit. SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01106. ATPase-IIC_X-K. 1 hit. TIGR01494. ATPase_P-type. 2 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	305134.
SOURCE	Search...

Entry information

Entry name

AT1A4_MOUSE

Accession

Primary (citable) accession number: Q9WV27
Secondary accession number(s): E9QKL5, Q9R173, Q9WV28

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 112 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents