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Q9WV27

- AT1A4_MOUSE

UniProt

Q9WV27 - AT1A4_MOUSE

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Protein
Sodium/potassium-transporting ATPase subunit alpha-4
Gene
Atp1a4, Atp1al2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Plays a role in sperm motility By similarity.

Catalytic activityi

ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

Enzyme regulationi

Specifically inhibited by an endogenous cardiac glycoside, ouabain By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei386 – 38614-aspartylphosphate intermediate By similarity
Metal bindingi726 – 7261Magnesium By similarity
Metal bindingi730 – 7301Magnesium By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. sodium:potassium-exchanging ATPase activity Source: MGI

GO - Biological processi

  1. ATP biosynthetic process Source: InterPro
  2. fertilization Source: MGI
  3. regulation of cellular pH Source: UniProtKB
  4. regulation of membrane potential Source: MGI
  5. sodium ion transmembrane transport Source: GOC
  6. sodium ion transport Source: MGI
  7. sperm motility Source: UniProtKB
  8. spermatogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/potassium-transporting ATPase subunit alpha-4 (EC:3.6.3.9)
Short name:
Na(+)/K(+) ATPase alpha-4 subunit
Alternative name(s):
Sodium pump subunit alpha-4
Gene namesi
Name:Atp1a4
Synonyms:Atp1al2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1351335. Atp1a4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9696Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei97 – 11721Helical; Reviewed prediction
Add
BLAST
Topological domaini118 – 14124Extracellular Reviewed prediction
Add
BLAST
Transmembranei142 – 16221Helical; Reviewed prediction
Add
BLAST
Topological domaini163 – 298136Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei299 – 31820Helical; Reviewed prediction
Add
BLAST
Topological domaini319 – 33012Extracellular Reviewed prediction
Add
BLAST
Transmembranei331 – 34818Helical; Reviewed prediction
Add
BLAST
Topological domaini349 – 781433Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei782 – 80120Helical; Reviewed prediction
Add
BLAST
Topological domaini802 – 81110Extracellular Reviewed prediction
Transmembranei812 – 83221Helical; Reviewed prediction
Add
BLAST
Topological domaini833 – 85220Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei853 – 87523Helical; Reviewed prediction
Add
BLAST
Topological domaini876 – 92752Extracellular Reviewed prediction
Add
BLAST
Transmembranei928 – 94720Helical; Reviewed prediction
Add
BLAST
Topological domaini948 – 96013Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei961 – 97919Helical; Reviewed prediction
Add
BLAST
Topological domaini980 – 99415Extracellular Reviewed prediction
Add
BLAST
Transmembranei995 – 101521Helical; Reviewed prediction
Add
BLAST
Topological domaini1016 – 103217Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10321032Sodium/potassium-transporting ATPase subunit alpha-4
PRO_0000046304Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei952 – 9521Phosphoserine; by PKA By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9WV27.
PaxDbiQ9WV27.
PRIDEiQ9WV27.

PTM databases

PhosphoSiteiQ9WV27.

Expressioni

Tissue specificityi

Expressed at high levels in the testis and at low levels in the epididymis.

Gene expression databases

BgeeiQ9WV27.
GenevestigatoriQ9WV27.

Interactioni

Subunit structurei

Composed of three subunits: alpha (catalytic), beta and gamma.

Protein-protein interaction databases

IntActiQ9WV27. 4 interactions.
MINTiMINT-4088554.

Structurei

3D structure databases

ProteinModelPortaliQ9WV27.
SMRiQ9WV27. Positions 39-1032.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni91 – 933Interaction with phosphoinositide-3 kinase By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
GeneTreeiENSGT00560000076866.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
InParanoidiQ9WV27.
KOiK01539.
OMAiKSSDTWF.
OrthoDBiEOG7327N0.
TreeFamiTF312838.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR005775. ATPase_P-typ_Na/K_IIC.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WV27-1 [UniParc]FASTAAdd to Basket

« Hide

MEPGKEKEVE APGELNQKPR PSTRSSTTNR QPKMKRRKKD LEELKKEVVM     50
DDHKLTLDEL SAKYSVDLTK GLSILEAQDI LFQNGPNVLT PPPTTPEWVK 100
FCRQLFGGFS LLLWTGACLC FLAYGIHVNY YKENANKDNL YLGIVLSAVV 150
IITGCFSYYQ EAKSSKIMES FKNMVPQQAL VIRDGEKMQI NVRDVVLGDL 200
VEVKGGDQIP ADIRVISAQG CKVDNSSLTG ESEPQSRCPD CTHENPLETR 250
NIIFFSTNCV EGTARGIVIA TGDYTVMGRI ASLTSGLQMG KTPIATEIEH 300
FIHLITAVAV FLGVSFFWLS IILGYTWLDA VIFLIGIIVA NVPEGLLATV 350
TVCLTLTAKR MARKNCLVKN LEAVETLGST STICSDKTGT LTQNRMTVAH 400
LWFDKTVYEA DTSEEQTTGK TFPKSSDTWF YLARIAGLCN RADFKPHQES 450
VPIAKRATTG DASESALLKF IEQSYNPVSE MRQKNPKVAE IPFNSTNKYQ 500
MSIHLLEDNS EAHVLLMKGA PERIFDFCSS FLLNGQEYPM DEEMKMDFQN 550
AYIELGGLGE RVLGFCFLNL PSNFSKGFQF NTDELNFPME NLCFAGLISM 600
IDPPRTAVPD AVSKCRSAGI KVIMVTGDHP ITAKAIAKSV GIISEGNDTA 650
EDIAARLNIP ISQVNNKSVK AIVVHGSELK DMESQQLDDI LKSYKEIVFA 700
RTSPQQKLII VEGCQRLGAI VAVTGDGVND SPALKKADIG IAMGITGSDV 750
SKQAADMILL DDNFASIVTG VEEGRLIFDN LKKSIAYTLT SNIPEITPFL 800
LFIILSIPLP LGTITILCID LGTDMVPAIS LAYESPESDI MKRLPRNPKT 850
DNLVNNRLIG MAYGQIGMIQ ALAGFFTYFV ILAENGFKPL DLLGIRLYWD 900
DTQLNDLEDS YGQQWTYEQR KVVEFTCQTA FFISIVIVQW ADLIICKTRR 950
NSLFKQGMKN KILIFGLLEE TVLAAFLSYV PGMDVSLRMY PLKINWWFCA 1000
LPYSVLIFVY DEIRKLIIRR RPGGWLEKET YY 1032
Length:1,032
Mass (Da):114,887
Last modified:July 27, 2011 - v3
Checksum:i5B97565BA67AA08C
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti74 – 741I → V in AAD43812. 1 Publication
Sequence conflicti476 – 4761N → S in AAD43813. 1 Publication
Sequence conflicti546 – 5461M → T in AAD43813. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF164348 Genomic DNA. Translation: AAD43812.1.
AF164349 Genomic DNA. Translation: AAD43813.1.
AF164350 Genomic DNA. Translation: AAD43814.1.
AC074310 Genomic DNA. No translation available.
AC087061 Genomic DNA. No translation available.
CCDSiCCDS48446.1.
RefSeqiNP_038762.1. NM_013734.1.
UniGeneiMm.482392.

Genome annotation databases

EnsembliENSMUST00000111243; ENSMUSP00000106874; ENSMUSG00000007107.
GeneIDi27222.
KEGGimmu:27222.
UCSCiuc011wwk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF164348 Genomic DNA. Translation: AAD43812.1 .
AF164349 Genomic DNA. Translation: AAD43813.1 .
AF164350 Genomic DNA. Translation: AAD43814.1 .
AC074310 Genomic DNA. No translation available.
AC087061 Genomic DNA. No translation available.
CCDSi CCDS48446.1.
RefSeqi NP_038762.1. NM_013734.1.
UniGenei Mm.482392.

3D structure databases

ProteinModelPortali Q9WV27.
SMRi Q9WV27. Positions 39-1032.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9WV27. 4 interactions.
MINTi MINT-4088554.

PTM databases

PhosphoSitei Q9WV27.

Proteomic databases

MaxQBi Q9WV27.
PaxDbi Q9WV27.
PRIDEi Q9WV27.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000111243 ; ENSMUSP00000106874 ; ENSMUSG00000007107 .
GeneIDi 27222.
KEGGi mmu:27222.
UCSCi uc011wwk.1. mouse.

Organism-specific databases

CTDi 480.
MGIi MGI:1351335. Atp1a4.

Phylogenomic databases

eggNOGi COG0474.
GeneTreei ENSGT00560000076866.
HOGENOMi HOG000265622.
HOVERGENi HBG004298.
InParanoidi Q9WV27.
KOi K01539.
OMAi KSSDTWF.
OrthoDBi EOG7327N0.
TreeFami TF312838.

Miscellaneous databases

NextBioi 305134.
PROi Q9WV27.
SOURCEi Search...

Gene expression databases

Bgeei Q9WV27.
Genevestigatori Q9WV27.

Family and domain databases

Gene3Di 1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProi IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR005775. ATPase_P-typ_Na/K_IIC.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
Pfami PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
SMARTi SM00831. Cation_ATPase_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsi TIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Na,K-ATPase alpha4 gene (Atp1a4) encodes a ouabain-resistant alpha subunit and is tightly linked to the alpha2 gene (Atp1a2) on mouse chromosome 1."
    Underhill D.A., Canfield V.A., Dahl J.P., Gros P., Levenson R.
    Biochemistry 38:14746-14751(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: C57BL/6J and CD-1.
    Tissue: Mammary gland and Testis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 173-183; 370-387; 488-498; 606-634; 708-716 AND 736-783, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiAT1A4_MOUSE
AccessioniPrimary (citable) accession number: Q9WV27
Secondary accession number(s): E9QKL5, Q9R173, Q9WV28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi