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Q9WV18 (GABR1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Gamma-aminobutyric acid type B receptor subunit 1

Short name=GABA-B receptor 1
Short name=GABA-B-R1
Short name=GABA-BR1
Short name=GABABR1
Short name=Gb1
Gene names
Name:Gabbr1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length960 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis. Calcium is required for high affinity binding to GABA. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. Ref.1

Subunit structure

Heterodimer of GABBR1 and GABBR2. Homodimers may form, but are inactive. Interacts with the leucine zipper of the C-terminal bZIP domain of ATF4 via its C-terminal region By similarity. Interacts with JAKMIP1. Ref.1 Ref.5 Ref.6

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity. Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein By similarity. Cell projectiondendrite By similarity. Note: Coexpression of GABBR1 and GABBR2 is required for GABBR1 maturation and transport to the plasma membrane. Colocalizes with ATF4 in hippocampal neuron dendritic membranes By similarity. Ref.1

Tissue specificity

Expressed in neuronal tissue including cortex, cerebellum and spinal cord. Not detected in non-neuronal tissues including heart, liver, spleen and kidney. Ref.2

Domain

Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABBR2. The linker region between the transmembrane domain 3 (TM3) and the transmembrane domain 4 (TM4) probably plays a role in the specificity for G-protein coupling.

Sequence similarities

Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.

Contains 2 Sushi (CCP/SCR) domains.

Ontologies

Keywords
   Cellular componentCell junction
Cell membrane
Cell projection
Membrane
Postsynaptic cell membrane
Synapse
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
Repeat
Signal
Sushi
Transmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processadenylate cyclase-inhibiting G-protein coupled receptor signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

gamma-aminobutyric acid signaling pathway

Inferred from direct assay PubMed 20400944. Source: MGI

   Cellular_componentG-protein coupled receptor dimeric complex

Inferred from direct assay PubMed 20400944. Source: MGI

G-protein coupled receptor heterodimeric complex

Inferred from sequence or structural similarity. Source: UniProtKB

cell junction

Inferred from electronic annotation. Source: UniProtKB-KW

cytoplasm

Inferred from direct assay PubMed 15013631. Source: MGI

dendrite

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

membrane

Inferred from direct assay PubMed 20400944. Source: MGI

postsynaptic membrane

Inferred from direct assay PubMed 20400944. Source: MGI

presynaptic membrane

Inferred from direct assay PubMed 20400944. Source: MGI

   Molecular_functionG-protein coupled GABA receptor activity

Inferred from sequence or structural similarity. Source: UniProtKB

protein binding

Inferred from physical interaction PubMed 15013631PubMed 20400944. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1A (identifier: Q9WV18-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1B (identifier: Q9WV18-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-163: MLLLLLVPLF...PHCQVNRTPH → MGPGGPCTPV...PHPRVPPHPS

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1616 Potential
Chain17 – 960944Gamma-aminobutyric acid type B receptor subunit 1
PRO_0000012950

Regions

Topological domain17 – 590574Extracellular Potential
Transmembrane591 – 61121Helical; Name=1; Potential
Topological domain612 – 63019Cytoplasmic Potential
Transmembrane631 – 65121Helical; Name=2; Potential
Topological domain652 – 66615Extracellular Potential
Transmembrane667 – 68721Helical; Name=3; Potential
Topological domain688 – 70922Cytoplasmic Potential
Transmembrane710 – 73021Helical; Name=4; Potential
Topological domain731 – 76737Extracellular Potential
Transmembrane768 – 78821Helical; Name=5; Potential
Topological domain789 – 80315Cytoplasmic Potential
Transmembrane804 – 82421Helical; Name=6; Potential
Topological domain825 – 8328Extracellular Potential
Transmembrane833 – 85321Helical; Name=7; Potential
Topological domain854 – 960107Cytoplasmic Potential
Domain29 – 9567Sushi 1
Domain97 – 15862Sushi 2
Region887 – 91529Interaction with ATF4 By similarity
Coiled coil868 – 92457 Potential

Sites

Binding site2461Agonist By similarity
Binding site2691Agonist By similarity
Binding site2861Agonist By similarity
Binding site3661Agonist By similarity
Binding site3941Agonist By similarity
Binding site4651Agonist By similarity

Amino acid modifications

Glycosylation231N-linked (GlcNAc...) Potential
Glycosylation831N-linked (GlcNAc...) Potential
Glycosylation4081N-linked (GlcNAc...) Potential
Glycosylation4391N-linked (GlcNAc...) Potential
Glycosylation4811N-linked (GlcNAc...) Potential
Glycosylation5011N-linked (GlcNAc...) Potential
Glycosylation5131N-linked (GlcNAc...) Potential
Disulfide bond99 ↔ 144 By similarity
Disulfide bond130 ↔ 156 By similarity
Disulfide bond219 ↔ 245 By similarity
Disulfide bond375 ↔ 409 By similarity

Natural variations

Alternative sequence1 – 163163MLLLL…NRTPH → MGPGGPCTPVGWPLPLLLVM AAGVAPVWASHSPHLPRPHP RVPPHPS in isoform 1B.
VSP_002041

Experimental info

Sequence conflict7 – 82VP → LL in AAD22194. Ref.1
Sequence conflict461T → I in AAD22194. Ref.1
Sequence conflict6181V → A in AAD22194. Ref.1
Sequence conflict6421A → V in AAD22194. Ref.1
Sequence conflict7001W → R in AAH56990. Ref.4
Sequence conflict7211I → V in AAD22194. Ref.1
Sequence conflict8121A → P in AAG29338. Ref.2
Sequence conflict8121A → P in AAG29341. Ref.2
Sequence conflict8691A → T in AAD22194. Ref.1
Sequence conflict9211I → L in AAD22194. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1A [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: E4B5A9401E23E8B4

FASTA960108,216
        10         20         30         40         50         60 
MLLLLLVPLF LRPLGAGGAQ TPNVTSEGCQ IIHPPWEGGI RYRGLTRDQV KAINFLPVDY 

        70         80         90        100        110        120 
EIEYVCRGER EVVGPKVRKC LANGSWTDMD TPSRCVRICS KSYLTLENGK VFLTGGDLPA 

       130        140        150        160        170        180 
LDGARVDFRC DPDFHLVGSS RSICSQGQWS TPKPHCQVNR TPHSERRAVY IGALFPMSGG 

       190        200        210        220        230        240 
WPGGQACQPA VEMALEDVNS RRDILPDYEL KLIHHDSKCD PGQATKYLYE LLYNDPIKII 

       250        260        270        280        290        300 
LMPGCSSVST LVAEAARMWN LIVLSYGSSS PALSNRQRFP TFFRTHPSAT LHNPTRVKLF 

       310        320        330        340        350        360 
EKWGWKKIAT IQQTTEVFTS TLDDLEERVK EAGIEITFRQ SFFSDPAVPV KNLKRQDARI 

       370        380        390        400        410        420 
IVGLFYETEA RKVFCEVYKE RLFGKKYVWF LIGWYADNWF KTYDPSINCT VEEMTEAVEG 

       430        440        450        460        470        480 
HITTEIVMLN PANTRSISNM TSQEFVEKLT KRLKRHPEET GGFQEAPLAY DAIWALALAL 

       490        500        510        520        530        540 
NKTSGGGGRS GVRLEDFNYN NQTITDQIYR AMNSSSFEGV SGHVVFDASG SRMAWTLIEQ 

       550        560        570        580        590        600 
LQGGSYKKIG YYDSTKDDLS WSKTDKWIGG SPPADQTLVI KTFRFLSQKL FISVSVLSSL 

       610        620        630        640        650        660 
GIVLAVVCLS FNIYNSHVRY IQNSQPNLNN LTAVGCSLAL AAVFPLGLDG YHIGRSQFPF 

       670        680        690        700        710        720 
VCQARLWLLG LGFSLGYGSM FTKIWWVHTV FTKKEEKKEW RKTLEPWKLY ATVGLLVGMD 

       730        740        750        760        770        780 
ILTLAIWQIV DPLHRTIETF AKEEPKEDID VSILPQLEHC SSKKMNTWLG IFYGYKGLLL 

       790        800        810        820        830        840 
LLGIFLAYET KSVSTEKIND HRAVGMAIYN VAVLCLITAP VTMILSSQQD AAFAFASLAI 

       850        860        870        880        890        900 
VFSSYITLVV LFVPKMRRLI TRGEWQSEAQ DTMKTGSSTN NNEEEKSRLL EKENRELEKI 

       910        920        930        940        950        960 
IAEKEERVSE LRHQLQSRQQ IRSRRHPPTP PDPSGGLPRG PSEPPDRLSC DGSRVHLLYK 

« Hide

Isoform 1B [UniParc].

Checksum: 76CA895396EAFDC0
Show »

FASTA84495,022

References

« Hide 'large scale' references
[1]"Coexpression of full-length gamma-aminobutyric acid(B) (GABA(B)) receptors with truncated receptors and metabotropic glutamate receptor 4 supports the GABA(B) heterodimer as the functional receptor."
Sullivan R., Chateauneuf A., Coulombe N., Kolakowski L.F. Jr., Johnson M.P., Hebert T.E., Ethier N., Belley M., Metters K., Abramovitz M., O'Neill G.P., Ng G.Y.K.
J. Pharmacol. Exp. Ther. 293:460-467(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1A), FUNCTION, INTERACTION WITH GABBR2, SUBCELLULAR LOCATION.
[2]"The murine GABA(B) receptor 1: cDNA cloning, tissue distribution, structure of the Gabbr1 gene, and mapping to chromosome 17."
Lamp K., Humeny A., Nikolic Z., Imai K., Adamski J., Schiebel K., Becker C.M.
Cytogenet. Cell Genet. 92:116-121(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1A AND 1B), TISSUE SPECIFICITY.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1A AND 1B).
Strain: C57BL/6.
Tissue: Brain.
[5]"Role of heteromer formation in GABAB receptor function."
Kuner R., Koehr G., Gruenewald S., Eisenhardt G., Bach A., Kornau H.-C.
Science 283:74-77(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GABBR2.
[6]"Marlin-1, a novel RNA-binding protein associates with GABA receptors."
Couve A., Restituito S., Brandon J.M., Charles K.J., Bawagan H., Freeman K.B., Pangalos M.N., Calver A.R., Moss S.J.
J. Biol. Chem. 279:13934-13943(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH JAKMIP1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF114168 mRNA. Translation: AAD22194.2.
AF008649 mRNA. Translation: AAG29338.1.
AF120255 mRNA. Translation: AAG29341.1.
AL078630 Genomic DNA. No translation available.
BC054735 mRNA. Translation: AAH54735.1.
BC056990 mRNA. Translation: AAH56990.1.
CCDSCCDS37616.1. [Q9WV18-1]
RefSeqNP_062312.3. NM_019439.3. [Q9WV18-1]
UniGeneMm.32191.

3D structure databases

ProteinModelPortalQ9WV18.
SMRQ9WV18. Positions 54-159, 164-575.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid207643. 3 interactions.
IntActQ9WV18. 3 interactions.
STRING10090.ENSMUSP00000025338.

Chemistry

GuidetoPHARMACOLOGY240.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ9WV18.

Proteomic databases

MaxQBQ9WV18.
PaxDbQ9WV18.
PRIDEQ9WV18.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000025338; ENSMUSP00000025338; ENSMUSG00000024462. [Q9WV18-1]
ENSMUST00000172792; ENSMUSP00000134268; ENSMUSG00000024462. [Q9WV18-2]
GeneID54393.
KEGGmmu:54393.
UCSCuc008cma.1. mouse. [Q9WV18-1]
uc008cmd.1. mouse. [Q9WV18-2]

Organism-specific databases

CTD2550.
MGIMGI:1860139. Gabbr1.

Phylogenomic databases

eggNOGCOG0683.
GeneTreeENSGT00530000063129.
HOGENOMHOG000016575.
HOVERGENHBG051688.
InParanoidQ6PGJ2.
KOK04615.
OMASTQFLGV.
OrthoDBEOG7D2FCV.
PhylomeDBQ9WV18.
TreeFamTF313965.

Gene expression databases

ArrayExpressQ9WV18.
BgeeQ9WV18.
CleanExMM_GABBR1.
GenevestigatorQ9WV18.

Family and domain databases

InterProIPR001828. ANF_lig-bd_rcpt.
IPR002455. GPCR3_GABA-B.
IPR017978. GPCR_3_C.
IPR002456. GPCR_3_GABA_rcpt_B1.
IPR028082. Peripla_BP_I.
IPR000436. Sushi_SCR_CCP.
[Graphical view]
PANTHERPTHR10519. PTHR10519. 1 hit.
PfamPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF00084. Sushi. 2 hits.
[Graphical view]
PRINTSPR01177. GABAB1RECPTR.
SMARTSM00032. CCP. 2 hits.
[Graphical view]
SUPFAMSSF53822. SSF53822. 1 hit.
SSF57535. SSF57535. 2 hits.
PROSITEPS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
PS50923. SUSHI. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSGABBR1. mouse.
NextBio311240.
PROQ9WV18.
SOURCESearch...

Entry information

Entry nameGABR1_MOUSE
AccessionPrimary (citable) accession number: Q9WV18
Secondary accession number(s): Q6PGJ2 expand/collapse secondary AC list , Q9WU48, Q9WV15, Q9WV16, Q9WV17
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1999
Last modified: July 9, 2014
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries