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Protein

Gamma-aminobutyric acid type B receptor subunit 1

Gene

Gabbr1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis. Calcium is required for high affinity binding to GABA. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei246AgonistBy similarity1
Binding sitei269AgonistBy similarity1
Binding sitei286AgonistBy similarity1
Binding sitei366AgonistBy similarity1
Binding sitei394AgonistBy similarity1
Binding sitei465AgonistBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-MMU-1296041. Activation of G protein gated Potassium channels.
R-MMU-418594. G alpha (i) signalling events.
R-MMU-420499. Class C/3 (Metabotropic glutamate/pheromone receptors).
R-MMU-977444. GABA B receptor activation.
R-MMU-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid type B receptor subunit 1
Short name:
GABA-B receptor 1
Short name:
GABA-B-R1
Short name:
GABA-BR1
Short name:
GABABR1
Short name:
Gb1
Gene namesi
Name:Gabbr1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1860139. Gabbr1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini17 – 590ExtracellularSequence analysisAdd BLAST574
Transmembranei591 – 611Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini612 – 630CytoplasmicSequence analysisAdd BLAST19
Transmembranei631 – 651Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini652 – 666ExtracellularSequence analysisAdd BLAST15
Transmembranei667 – 687Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini688 – 709CytoplasmicSequence analysisAdd BLAST22
Transmembranei710 – 730Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini731 – 767ExtracellularSequence analysisAdd BLAST37
Transmembranei768 – 788Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini789 – 803CytoplasmicSequence analysisAdd BLAST15
Transmembranei804 – 824Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini825 – 832ExtracellularSequence analysis8
Transmembranei833 – 853Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini854 – 960CytoplasmicSequence analysisAdd BLAST107

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cytoplasm Source: MGI
  • dendrite Source: UniProtKB-SubCell
  • G-protein coupled receptor dimeric complex Source: MGI
  • G-protein coupled receptor heterodimeric complex Source: UniProtKB
  • integral component of plasma membrane Source: UniProtKB
  • membrane Source: MGI
  • postsynaptic membrane Source: MGI
  • presynaptic membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
ChainiPRO_000001295017 – 960Gamma-aminobutyric acid type B receptor subunit 1Add BLAST944

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi23N-linked (GlcNAc...)Sequence analysis1
Glycosylationi83N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi99 ↔ 144PROSITE-ProRule annotation
Disulfide bondi130 ↔ 156PROSITE-ProRule annotation
Disulfide bondi219 ↔ 245PROSITE-ProRule annotation
Disulfide bondi375 ↔ 409PROSITE-ProRule annotation
Glycosylationi408N-linked (GlcNAc...)Sequence analysis1
Glycosylationi439N-linked (GlcNAc...)Sequence analysis1
Glycosylationi481N-linked (GlcNAc...)Sequence analysis1
Glycosylationi501N-linked (GlcNAc...)Sequence analysis1
Glycosylationi513N-linked (GlcNAc...)Sequence analysis1
Modified residuei872PhosphothreonineCombined sources1
Modified residuei929PhosphothreonineCombined sources1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9WV18.
PeptideAtlasiQ9WV18.
PRIDEiQ9WV18.

PTM databases

iPTMnetiQ9WV18.
PhosphoSitePlusiQ9WV18.

Expressioni

Tissue specificityi

Expressed in neuronal tissue including cortex, cerebellum and spinal cord. Not detected in non-neuronal tissues including heart, liver, spleen and kidney.1 Publication

Gene expression databases

BgeeiENSMUSG00000024462.
CleanExiMM_GABBR1.
ExpressionAtlasiQ9WV18. baseline and differential.
GenevisibleiQ9WV18. MM.

Interactioni

Subunit structurei

Heterodimer of GABBR1 and GABBR2. Homodimers may form, but are inactive. Interacts with the leucine zipper of the C-terminal bZIP domain of ATF4 via its C-terminal region (By similarity). Interacts with JAKMIP1.By similarity3 Publications

Protein-protein interaction databases

BioGridi207643. 3 interactors.
IntActiQ9WV18. 3 interactors.
STRINGi10090.ENSMUSP00000025338.

Structurei

3D structure databases

ProteinModelPortaliQ9WV18.
SMRiQ9WV18.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 95Sushi 1PROSITE-ProRule annotationAdd BLAST67
Domaini97 – 158Sushi 2PROSITE-ProRule annotationAdd BLAST62

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni887 – 915Interaction with ATF4By similarityAdd BLAST29

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili868 – 924Sequence analysisAdd BLAST57

Domaini

Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABBR2. The linker region between the transmembrane domain 3 (TM3) and the transmembrane domain 4 (TM4) probably plays a role in the specificity for G-protein coupling.

Sequence similaritiesi

Contains 2 Sushi (CCP/SCR) domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, Signal, Sushi, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1055. Eukaryota.
ENOG410XNN1. LUCA.
GeneTreeiENSGT00530000063129.
HOGENOMiHOG000016575.
HOVERGENiHBG051688.
InParanoidiQ9WV18.
KOiK04615.
OMAiPKICQAR.
OrthoDBiEOG091G01WG.
PhylomeDBiQ9WV18.
TreeFamiTF313965.

Family and domain databases

CDDicd00033. CCP. 1 hit.
InterProiIPR001828. ANF_lig-bd_rcpt.
IPR002455. GPCR3_GABA-B.
IPR017978. GPCR_3_C.
IPR002456. GPCR_3_GABA_rcpt_B1.
IPR028082. Peripla_BP_I.
IPR000436. Sushi_SCR_CCP_dom.
[Graphical view]
PANTHERiPTHR10519. PTHR10519. 1 hit.
PTHR10519:SF42. PTHR10519:SF42. 1 hit.
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF00084. Sushi. 1 hit.
[Graphical view]
PRINTSiPR01177. GABAB1RECPTR.
SMARTiSM00032. CCP. 2 hits.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
SSF57535. SSF57535. 2 hits.
PROSITEiPS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
PS50923. SUSHI. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1A (identifier: Q9WV18-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLLLLVPLF LRPLGAGGAQ TPNVTSEGCQ IIHPPWEGGI RYRGLTRDQV
60 70 80 90 100
KAINFLPVDY EIEYVCRGER EVVGPKVRKC LANGSWTDMD TPSRCVRICS
110 120 130 140 150
KSYLTLENGK VFLTGGDLPA LDGARVDFRC DPDFHLVGSS RSICSQGQWS
160 170 180 190 200
TPKPHCQVNR TPHSERRAVY IGALFPMSGG WPGGQACQPA VEMALEDVNS
210 220 230 240 250
RRDILPDYEL KLIHHDSKCD PGQATKYLYE LLYNDPIKII LMPGCSSVST
260 270 280 290 300
LVAEAARMWN LIVLSYGSSS PALSNRQRFP TFFRTHPSAT LHNPTRVKLF
310 320 330 340 350
EKWGWKKIAT IQQTTEVFTS TLDDLEERVK EAGIEITFRQ SFFSDPAVPV
360 370 380 390 400
KNLKRQDARI IVGLFYETEA RKVFCEVYKE RLFGKKYVWF LIGWYADNWF
410 420 430 440 450
KTYDPSINCT VEEMTEAVEG HITTEIVMLN PANTRSISNM TSQEFVEKLT
460 470 480 490 500
KRLKRHPEET GGFQEAPLAY DAIWALALAL NKTSGGGGRS GVRLEDFNYN
510 520 530 540 550
NQTITDQIYR AMNSSSFEGV SGHVVFDASG SRMAWTLIEQ LQGGSYKKIG
560 570 580 590 600
YYDSTKDDLS WSKTDKWIGG SPPADQTLVI KTFRFLSQKL FISVSVLSSL
610 620 630 640 650
GIVLAVVCLS FNIYNSHVRY IQNSQPNLNN LTAVGCSLAL AAVFPLGLDG
660 670 680 690 700
YHIGRSQFPF VCQARLWLLG LGFSLGYGSM FTKIWWVHTV FTKKEEKKEW
710 720 730 740 750
RKTLEPWKLY ATVGLLVGMD ILTLAIWQIV DPLHRTIETF AKEEPKEDID
760 770 780 790 800
VSILPQLEHC SSKKMNTWLG IFYGYKGLLL LLGIFLAYET KSVSTEKIND
810 820 830 840 850
HRAVGMAIYN VAVLCLITAP VTMILSSQQD AAFAFASLAI VFSSYITLVV
860 870 880 890 900
LFVPKMRRLI TRGEWQSEAQ DTMKTGSSTN NNEEEKSRLL EKENRELEKI
910 920 930 940 950
IAEKEERVSE LRHQLQSRQQ IRSRRHPPTP PDPSGGLPRG PSEPPDRLSC
960
DGSRVHLLYK
Length:960
Mass (Da):108,216
Last modified:November 1, 1999 - v1
Checksum:iE4B5A9401E23E8B4
GO
Isoform 1B (identifier: Q9WV18-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-163: MLLLLLVPLF...PHCQVNRTPH → MGPGGPCTPV...PHPRVPPHPS

Show »
Length:844
Mass (Da):95,022
Checksum:i76CA895396EAFDC0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7 – 8VP → LL in AAD22194 (PubMed:10773016).Curated2
Sequence conflicti46T → I in AAD22194 (PubMed:10773016).Curated1
Sequence conflicti618V → A in AAD22194 (PubMed:10773016).Curated1
Sequence conflicti642A → V in AAD22194 (PubMed:10773016).Curated1
Sequence conflicti700W → R in AAH56990 (PubMed:15489334).Curated1
Sequence conflicti721I → V in AAD22194 (PubMed:10773016).Curated1
Sequence conflicti812A → P in AAG29338 (PubMed:11306808).Curated1
Sequence conflicti812A → P in AAG29341 (PubMed:11306808).Curated1
Sequence conflicti869A → T in AAD22194 (PubMed:10773016).Curated1
Sequence conflicti921I → L in AAD22194 (PubMed:10773016).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0020411 – 163MLLLL…NRTPH → MGPGGPCTPVGWPLPLLLVM AAGVAPVWASHSPHLPRPHP RVPPHPS in isoform 1B. 2 PublicationsAdd BLAST163

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF114168 mRNA. Translation: AAD22194.2.
AF008649 mRNA. Translation: AAG29338.1.
AF120255 mRNA. Translation: AAG29341.1.
AL078630 Genomic DNA. No translation available.
BC054735 mRNA. Translation: AAH54735.1.
BC056990 mRNA. Translation: AAH56990.1.
CCDSiCCDS37616.1. [Q9WV18-1]
RefSeqiNP_062312.3. NM_019439.3. [Q9WV18-1]
UniGeneiMm.32191.

Genome annotation databases

EnsembliENSMUST00000025338; ENSMUSP00000025338; ENSMUSG00000024462. [Q9WV18-1]
ENSMUST00000172792; ENSMUSP00000134268; ENSMUSG00000024462. [Q9WV18-2]
GeneIDi54393.
KEGGimmu:54393.
UCSCiuc008cma.1. mouse. [Q9WV18-1]
uc008cmd.1. mouse. [Q9WV18-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF114168 mRNA. Translation: AAD22194.2.
AF008649 mRNA. Translation: AAG29338.1.
AF120255 mRNA. Translation: AAG29341.1.
AL078630 Genomic DNA. No translation available.
BC054735 mRNA. Translation: AAH54735.1.
BC056990 mRNA. Translation: AAH56990.1.
CCDSiCCDS37616.1. [Q9WV18-1]
RefSeqiNP_062312.3. NM_019439.3. [Q9WV18-1]
UniGeneiMm.32191.

3D structure databases

ProteinModelPortaliQ9WV18.
SMRiQ9WV18.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207643. 3 interactors.
IntActiQ9WV18. 3 interactors.
STRINGi10090.ENSMUSP00000025338.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiQ9WV18.
PhosphoSitePlusiQ9WV18.

Proteomic databases

PaxDbiQ9WV18.
PeptideAtlasiQ9WV18.
PRIDEiQ9WV18.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025338; ENSMUSP00000025338; ENSMUSG00000024462. [Q9WV18-1]
ENSMUST00000172792; ENSMUSP00000134268; ENSMUSG00000024462. [Q9WV18-2]
GeneIDi54393.
KEGGimmu:54393.
UCSCiuc008cma.1. mouse. [Q9WV18-1]
uc008cmd.1. mouse. [Q9WV18-2]

Organism-specific databases

CTDi2550.
MGIiMGI:1860139. Gabbr1.

Phylogenomic databases

eggNOGiKOG1055. Eukaryota.
ENOG410XNN1. LUCA.
GeneTreeiENSGT00530000063129.
HOGENOMiHOG000016575.
HOVERGENiHBG051688.
InParanoidiQ9WV18.
KOiK04615.
OMAiPKICQAR.
OrthoDBiEOG091G01WG.
PhylomeDBiQ9WV18.
TreeFamiTF313965.

Enzyme and pathway databases

ReactomeiR-MMU-1296041. Activation of G protein gated Potassium channels.
R-MMU-418594. G alpha (i) signalling events.
R-MMU-420499. Class C/3 (Metabotropic glutamate/pheromone receptors).
R-MMU-977444. GABA B receptor activation.
R-MMU-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Miscellaneous databases

ChiTaRSiGabbr1. mouse.
PROiQ9WV18.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024462.
CleanExiMM_GABBR1.
ExpressionAtlasiQ9WV18. baseline and differential.
GenevisibleiQ9WV18. MM.

Family and domain databases

CDDicd00033. CCP. 1 hit.
InterProiIPR001828. ANF_lig-bd_rcpt.
IPR002455. GPCR3_GABA-B.
IPR017978. GPCR_3_C.
IPR002456. GPCR_3_GABA_rcpt_B1.
IPR028082. Peripla_BP_I.
IPR000436. Sushi_SCR_CCP_dom.
[Graphical view]
PANTHERiPTHR10519. PTHR10519. 1 hit.
PTHR10519:SF42. PTHR10519:SF42. 1 hit.
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF00084. Sushi. 1 hit.
[Graphical view]
PRINTSiPR01177. GABAB1RECPTR.
SMARTiSM00032. CCP. 2 hits.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
SSF57535. SSF57535. 2 hits.
PROSITEiPS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
PS50923. SUSHI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGABR1_MOUSE
AccessioniPrimary (citable) accession number: Q9WV18
Secondary accession number(s): Q6PGJ2
, Q9WU48, Q9WV15, Q9WV16, Q9WV17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1999
Last modified: November 30, 2016
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.