Q9WV18 (GABR1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-aminobutyric acid type B receptor subunit 1
Short name=GABA-B receptor 1
Short name=GABA-B-R1
Short name=GABA-BR1
Short name=GABABR1
Short name=Gb1

Gene names

Name:

Gabbr1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	960 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception.
Cofactor	Calcium. Required for high affinity binding to GABA By similarity.
Subunit structure	Heterodimer of GABA-B-R1 and GABA-B-R2. Neither of which is effective on its own and homodimeric assembly does not seem to happen. Interacts with the leucine zipper of the C-terminal bZIP domain of ATF4 via its C-terminal region By similarity. Interacts with JAKMIP1. Ref.5 Ref.6
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein By similarity. Note: Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane By similarity. Colocalizes with ATF4 in hippocampal neuron dendritic membranes By similarity.
Tissue specificity	Expressed in neuronal tissue including cortex, cerebellum and spinal cord. Not detected in non-neuronal tissues including heart, liver, spleen and kidney. Ref.2
Domain	Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 2. The linker region between the transmembrane domain 3 (TM3) and the transmembrane domain 4 (TM4) probably play a role in the specificity for G-protein coupling.
Sequence similarities	Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily. Contains 2 Sushi (CCP/SCR) domains.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Repeat Signal Sushi Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	gamma-aminobutyric acid signaling pathway Inferred from direct assay PubMed 20400944. Source: MGI
Cellular_component	G-protein coupled receptor dimeric complex Inferred from direct assay PubMed 20400944. Source: MGI cell junction Inferred from electronic annotation. Source: UniProtKB-KW cytoplasm Inferred from direct assay PubMed 15013631. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from direct assay PubMed 20400944. Source: MGI presynaptic membrane Inferred from direct assay PubMed 20400944. Source: MGI
Molecular_function	G-protein coupled GABA receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1A (identifier: Q9WV18-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1B (identifier: Q9WV18-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-163: MLLLLLVPLF...PHCQVNRTPH → MGPGGPCTPV...PHPRVPPHPS

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 960	944	Gamma-aminobutyric acid type B receptor subunit 1		PRO_0000012950
Regions
	Topological domain	17 – 590	574	Extracellular Potential
	Transmembrane	591 – 611	21	Helical; Name=1; Potential
	Topological domain	612 – 630	19	Cytoplasmic Potential
	Transmembrane	631 – 651	21	Helical; Name=2; Potential
	Topological domain	652 – 666	15	Extracellular Potential
	Transmembrane	667 – 687	21	Helical; Name=3; Potential
	Topological domain	688 – 709	22	Cytoplasmic Potential
	Transmembrane	710 – 730	21	Helical; Name=4; Potential
	Topological domain	731 – 767	37	Extracellular Potential
	Transmembrane	768 – 788	21	Helical; Name=5; Potential
	Topological domain	789 – 803	15	Cytoplasmic Potential
	Transmembrane	804 – 824	21	Helical; Name=6; Potential
	Topological domain	825 – 832	8	Extracellular Potential
	Transmembrane	833 – 853	21	Helical; Name=7; Potential
	Topological domain	854 – 960	107	Cytoplasmic Potential
	Domain	29 – 95	67	Sushi 1
	Domain	97 – 158	62	Sushi 2
	Region	887 – 915	29	Interaction with ATF4 By similarity
	Coiled coil	868 – 924	57	Potential
Amino acid modifications
	Modified residue	929	1	Phosphothreonine Ref.7 Ref.8
	Glycosylation	23	1	N-linked (GlcNAc...) Potential
	Glycosylation	83	1	N-linked (GlcNAc...) Potential
	Glycosylation	408	1	N-linked (GlcNAc...) Potential
	Glycosylation	439	1	N-linked (GlcNAc...) Potential
	Glycosylation	481	1	N-linked (GlcNAc...) Potential
	Glycosylation	501	1	N-linked (GlcNAc...) Potential
	Glycosylation	513	1	N-linked (GlcNAc...) Potential
	Disulfide bond	99 ↔ 144		By similarity
	Disulfide bond	130 ↔ 156		By similarity
Natural variations
	Alternative sequence	1 – 163	163	MLLLL…NRTPH → MGPGGPCTPVGWPLPLLLVM AAGVAPVWASHSPHLPRPHP RVPPHPS in isoform 1B.		VSP_002041
Experimental info
	Sequence conflict	7 – 8	2	VP → LL in AAD22194. Ref.1
	Sequence conflict	46	1	T → I in AAD22194. Ref.1
	Sequence conflict	618	1	V → A in AAD22194. Ref.1
	Sequence conflict	642	1	A → V in AAD22194. Ref.1
	Sequence conflict	700	1	W → R in AAH56990. Ref.4
	Sequence conflict	721	1	I → V in AAD22194. Ref.1
	Sequence conflict	812	1	A → P in AAG29338. Ref.2
	Sequence conflict	812	1	A → P in AAG29341. Ref.2
	Sequence conflict	869	1	A → T in AAD22194. Ref.1
	Sequence conflict	921	1	I → L in AAD22194. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1A [UniParc]. Last modified November 1, 1999. Version 1. Checksum: E4B5A9401E23E8B4	FASTA	960	108,216
	10 20 30 40 50 60 MLLLLLVPLF LRPLGAGGAQ TPNVTSEGCQ IIHPPWEGGI RYRGLTRDQV KAINFLPVDY 70 80 90 100 110 120 EIEYVCRGER EVVGPKVRKC LANGSWTDMD TPSRCVRICS KSYLTLENGK VFLTGGDLPA 130 140 150 160 170 180 LDGARVDFRC DPDFHLVGSS RSICSQGQWS TPKPHCQVNR TPHSERRAVY IGALFPMSGG 190 200 210 220 230 240 WPGGQACQPA VEMALEDVNS RRDILPDYEL KLIHHDSKCD PGQATKYLYE LLYNDPIKII 250 260 270 280 290 300 LMPGCSSVST LVAEAARMWN LIVLSYGSSS PALSNRQRFP TFFRTHPSAT LHNPTRVKLF 310 320 330 340 350 360 EKWGWKKIAT IQQTTEVFTS TLDDLEERVK EAGIEITFRQ SFFSDPAVPV KNLKRQDARI 370 380 390 400 410 420 IVGLFYETEA RKVFCEVYKE RLFGKKYVWF LIGWYADNWF KTYDPSINCT VEEMTEAVEG 430 440 450 460 470 480 HITTEIVMLN PANTRSISNM TSQEFVEKLT KRLKRHPEET GGFQEAPLAY DAIWALALAL 490 500 510 520 530 540 NKTSGGGGRS GVRLEDFNYN NQTITDQIYR AMNSSSFEGV SGHVVFDASG SRMAWTLIEQ 550 560 570 580 590 600 LQGGSYKKIG YYDSTKDDLS WSKTDKWIGG SPPADQTLVI KTFRFLSQKL FISVSVLSSL 610 620 630 640 650 660 GIVLAVVCLS FNIYNSHVRY IQNSQPNLNN LTAVGCSLAL AAVFPLGLDG YHIGRSQFPF 670 680 690 700 710 720 VCQARLWLLG LGFSLGYGSM FTKIWWVHTV FTKKEEKKEW RKTLEPWKLY ATVGLLVGMD 730 740 750 760 770 780 ILTLAIWQIV DPLHRTIETF AKEEPKEDID VSILPQLEHC SSKKMNTWLG IFYGYKGLLL 790 800 810 820 830 840 LLGIFLAYET KSVSTEKIND HRAVGMAIYN VAVLCLITAP VTMILSSQQD AAFAFASLAI 850 860 870 880 890 900 VFSSYITLVV LFVPKMRRLI TRGEWQSEAQ DTMKTGSSTN NNEEEKSRLL EKENRELEKI 910 920 930 940 950 960 IAEKEERVSE LRHQLQSRQQ IRSRRHPPTP PDPSGGLPRG PSEPPDRLSC DGSRVHLLYK
	Isoform 1B [UniParc]. Checksum: 76CA895396EAFDC0	FASTA	844	95,022
	10 20 30 40 50 60 MGPGGPCTPV GWPLPLLLVM AAGVAPVWAS HSPHLPRPHP RVPPHPSSER RAVYIGALFP 70 80 90 100 110 120 MSGGWPGGQA CQPAVEMALE DVNSRRDILP DYELKLIHHD SKCDPGQATK YLYELLYNDP 130 140 150 160 170 180 IKIILMPGCS SVSTLVAEAA RMWNLIVLSY GSSSPALSNR QRFPTFFRTH PSATLHNPTR 190 200 210 220 230 240 VKLFEKWGWK KIATIQQTTE VFTSTLDDLE ERVKEAGIEI TFRQSFFSDP AVPVKNLKRQ 250 260 270 280 290 300 DARIIVGLFY ETEARKVFCE VYKERLFGKK YVWFLIGWYA DNWFKTYDPS INCTVEEMTE 310 320 330 340 350 360 AVEGHITTEI VMLNPANTRS ISNMTSQEFV EKLTKRLKRH PEETGGFQEA PLAYDAIWAL 370 380 390 400 410 420 ALALNKTSGG GGRSGVRLED FNYNNQTITD QIYRAMNSSS FEGVSGHVVF DASGSRMAWT 430 440 450 460 470 480 LIEQLQGGSY KKIGYYDSTK DDLSWSKTDK WIGGSPPADQ TLVIKTFRFL SQKLFISVSV 490 500 510 520 530 540 LSSLGIVLAV VCLSFNIYNS HVRYIQNSQP NLNNLTAVGC SLALAAVFPL GLDGYHIGRS 550 560 570 580 590 600 QFPFVCQARL WLLGLGFSLG YGSMFTKIWW VHTVFTKKEE KKEWRKTLEP WKLYATVGLL 610 620 630 640 650 660 VGMDILTLAI WQIVDPLHRT IETFAKEEPK EDIDVSILPQ LEHCSSKKMN TWLGIFYGYK 670 680 690 700 710 720 GLLLLLGIFL AYETKSVSTE KINDHRAVGM AIYNVAVLCL ITAPVTMILS SQQDAAFAFA 730 740 750 760 770 780 SLAIVFSSYI TLVVLFVPKM RRLITRGEWQ SEAQDTMKTG SSTNNNEEEK SRLLEKENRE 790 800 810 820 830 840 LEKIIAEKEE RVSELRHQLQ SRQQIRSRRH PPTPPDPSGG LPRGPSEPPD RLSCDGSRVH LLYK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Coexpression of full-length gamma-aminobutyric acid(B) (GABA(B)) receptors with truncated receptors and metabotropic glutamate receptor 4 supports the GABA(B) heterodimer as the functional receptor." Sullivan R., Chateauneuf A., Coulombe N., Kolakowski L.F. Jr., Johnson M.P., Hebert T.E., Ethier N., Belley M., Metters K., Abramovitz M., O'Neill G.P., Ng G.Y.K. J. Pharmacol. Exp. Ther. 293:460-467(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1A).
[2]	"The murine GABA(B) receptor 1: cDNA cloning, tissue distribution, structure of the Gabbr1 gene, and mapping to chromosome 17." Lamp K., Humeny A., Nikolic Z., Imai K., Adamski J., Schiebel K., Becker C.M. Cytogenet. Cell Genet. 92:116-121(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1A AND 1B), TISSUE SPECIFICITY.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1A AND 1B). Strain: C57BL/6. Tissue: Brain.
[5]	"Role of heteromer formation in GABAB receptor function." Kuner R., Koehr G., Gruenewald S., Eisenhardt G., Bach A., Kornau H.-C. Science 283:74-77(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GABBR2.
[6]	"Marlin-1, a novel RNA-binding protein associates with GABA receptors." Couve A., Restituito S., Brandon J.M., Charles K.J., Bawagan H., Freeman K.B., Pangalos M.N., Calver A.R., Moss S.J. J. Biol. Chem. 279:13934-13943(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH JAKMIP1.
[7]	"Comprehensive identification of phosphorylation sites in postsynaptic density preparations." Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L. Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-929, MASS SPECTROMETRY. Tissue: Brain.
[8]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-929, MASS SPECTROMETRY. Tissue: Brain cortex.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF114168 mRNA. Translation: AAD22194.2. AF008649 mRNA. Translation: AAG29338.1. AF120255 mRNA. Translation: AAG29341.1. AL078630 Genomic DNA. No translation available. BC054735 mRNA. Translation: AAH54735.1. BC056990 mRNA. Translation: AAH56990.1.
IPI	IPI00223325. IPI00755301.
RefSeq	NP_062312.3. NM_019439.3.
UniGene	Mm.32191.
3D structure databases
ProteinModelPortal	Q9WV18.
SMR	Q9WV18. Positions 54-159, 184-573.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9WV18. 2 interactions.
STRING	10090.ENSMUSP00000025338.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q9WV18.
Proteomic databases
PaxDb	Q9WV18.
PRIDE	Q9WV18.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000025338; ENSMUSP00000025338; ENSMUSG00000024462. ENSMUST00000172792; ENSMUSP00000134268; ENSMUSG00000024462.
GeneID	54393.
KEGG	mmu:54393.
UCSC	uc008cma.1. mouse. uc008cmd.1. mouse.
Organism-specific databases
CTD	2550.
MGI	MGI:1860139. Gabbr1.
Phylogenomic databases
eggNOG	COG0683.
GeneTree	ENSGT00530000063129.
HOGENOM	HOG000016575.
HOVERGEN	HBG051688.
InParanoid	Q6PGJ2.
KO	K04615.
OMA	SEPPDRL.
OrthoDB	EOG4BG8VH.
Gene expression databases
ArrayExpress	Q9WV18.
Bgee	Q9WV18.
CleanEx	MM_GABBR1.
Genevestigator	Q9WV18.
GermOnline	ENSMUSG00000024462. Mus musculus.
Family and domain databases
InterPro	IPR001828. ANF_lig-bd_rcpt. IPR017978. GPCR_3_C. IPR002455. GPCR_3_GABA_rcpt_B. IPR002456. GPCR_3_GABA_rcpt_B1. IPR000436. Sushi_SCR_CCP. [Graphical view]
Pfam	PF00003. 7tm_3. 1 hit. PF01094. ANF_receptor. 1 hit. PF00084. Sushi. 2 hits. [Graphical view]
PRINTS	PR01177. GABAB1RECPTR. PR01176. GABABRECEPTR.
SMART	SM00032. CCP. 2 hits. [Graphical view]
SUPFAM	SSF57535. Complement_control_module. 2 hits.
PROSITE	PS00979. G_PROTEIN_RECEP_F3_1. False negative. PS00980. G_PROTEIN_RECEP_F3_2. False negative. PS00981. G_PROTEIN_RECEP_F3_3. False negative. PS50259. G_PROTEIN_RECEP_F3_4. 1 hit. PS50923. SUSHI. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	GABBR1. mouse.
NextBio	311240.
SOURCE	Search...

Entry information

Entry name

GABR1_MOUSE

Accession

Primary (citable) accession number: Q9WV18
Secondary accession number(s): Q6PGJ2 , Q9WU48, Q9WV15, Q9WV16, Q9WV17

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	November 1, 1999
Last modified:	May 1, 2013
This is version 124 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program