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Q9WV18

- GABR1_MOUSE

UniProt

Q9WV18 - GABR1_MOUSE

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Protein

Gamma-aminobutyric acid type B receptor subunit 1

Gene

Gabbr1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis. Calcium is required for high affinity binding to GABA. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei246 – 2461AgonistBy similarity
Binding sitei269 – 2691AgonistBy similarity
Binding sitei286 – 2861AgonistBy similarity
Binding sitei366 – 3661AgonistBy similarity
Binding sitei394 – 3941AgonistBy similarity
Binding sitei465 – 4651AgonistBy similarity

GO - Molecular functioni

  1. G-protein coupled GABA receptor activity Source: UniProtKB

GO - Biological processi

  1. adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Source: UniProtKB
  2. gamma-aminobutyric acid signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_208837. Activation of G protein gated Potassium channels.
REACT_217868. GABA B receptor activation.
REACT_226457. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.
REACT_250376. G alpha (i) signalling events.
REACT_262510. Class C/3 (Metabotropic glutamate/pheromone receptors).

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid type B receptor subunit 1
Short name:
GABA-B receptor 1
Short name:
GABA-B-R1
Short name:
GABA-BR1
Short name:
GABABR1
Short name:
Gb1
Gene namesi
Name:Gabbr1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:1860139. Gabbr1.

Subcellular locationi

Cell membrane By similarity; Multi-pass membrane protein By similarity. Cell junctionsynapsepostsynaptic cell membrane By similarity; Multi-pass membrane protein By similarity. Cell projectiondendrite By similarity
Note: Coexpression of GABBR1 and GABBR2 is required for GABBR1 maturation and transport to the plasma membrane. Colocalizes with ATF4 in hippocampal neuron dendritic membranes (By similarity).By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini17 – 590574ExtracellularSequence AnalysisAdd
BLAST
Transmembranei591 – 61121Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini612 – 63019CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei631 – 65121Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini652 – 66615ExtracellularSequence AnalysisAdd
BLAST
Transmembranei667 – 68721Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini688 – 70922CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei710 – 73021Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini731 – 76737ExtracellularSequence AnalysisAdd
BLAST
Transmembranei768 – 78821Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini789 – 80315CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei804 – 82421Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini825 – 8328ExtracellularSequence Analysis
Transmembranei833 – 85321Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini854 – 960107CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. cell projection Source: UniProtKB-KW
  3. cytoplasm Source: MGI
  4. G-protein coupled receptor dimeric complex Source: MGI
  5. G-protein coupled receptor heterodimeric complex Source: UniProtKB
  6. integral component of plasma membrane Source: UniProtKB
  7. membrane Source: MGI
  8. postsynaptic membrane Source: MGI
  9. presynaptic membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Sequence AnalysisAdd
BLAST
Chaini17 – 960944Gamma-aminobutyric acid type B receptor subunit 1PRO_0000012950Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi23 – 231N-linked (GlcNAc...)Sequence Analysis
Glycosylationi83 – 831N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi99 ↔ 144PROSITE-ProRule annotation
Disulfide bondi130 ↔ 156PROSITE-ProRule annotation
Disulfide bondi219 ↔ 245PROSITE-ProRule annotation
Disulfide bondi375 ↔ 409PROSITE-ProRule annotation
Glycosylationi408 – 4081N-linked (GlcNAc...)Sequence Analysis
Glycosylationi439 – 4391N-linked (GlcNAc...)Sequence Analysis
Glycosylationi481 – 4811N-linked (GlcNAc...)Sequence Analysis
Glycosylationi501 – 5011N-linked (GlcNAc...)Sequence Analysis
Glycosylationi513 – 5131N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9WV18.
PaxDbiQ9WV18.
PRIDEiQ9WV18.

PTM databases

PhosphoSiteiQ9WV18.

Expressioni

Tissue specificityi

Expressed in neuronal tissue including cortex, cerebellum and spinal cord. Not detected in non-neuronal tissues including heart, liver, spleen and kidney.1 Publication

Gene expression databases

BgeeiQ9WV18.
CleanExiMM_GABBR1.
ExpressionAtlasiQ9WV18. baseline and differential.
GenevestigatoriQ9WV18.

Interactioni

Subunit structurei

Heterodimer of GABBR1 and GABBR2. Homodimers may form, but are inactive. Interacts with the leucine zipper of the C-terminal bZIP domain of ATF4 via its C-terminal region (By similarity). Interacts with JAKMIP1.By similarity3 Publications

Protein-protein interaction databases

BioGridi207643. 3 interactions.
IntActiQ9WV18. 3 interactions.
STRINGi10090.ENSMUSP00000025338.

Structurei

3D structure databases

ProteinModelPortaliQ9WV18.
SMRiQ9WV18. Positions 54-159, 164-575, 883-917.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 9567Sushi 1PROSITE-ProRule annotationAdd
BLAST
Domaini97 – 15862Sushi 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni887 – 91529Interaction with ATF4By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili868 – 92457Sequence AnalysisAdd
BLAST

Domaini

Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABBR2. The linker region between the transmembrane domain 3 (TM3) and the transmembrane domain 4 (TM4) probably plays a role in the specificity for G-protein coupling.

Sequence similaritiesi

Contains 2 Sushi (CCP/SCR) domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, Signal, Sushi, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0683.
GeneTreeiENSGT00530000063129.
HOGENOMiHOG000016575.
HOVERGENiHBG051688.
InParanoidiQ9WV18.
KOiK04615.
OMAiSTQFLGV.
OrthoDBiEOG7D2FCV.
PhylomeDBiQ9WV18.
TreeFamiTF313965.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR002455. GPCR3_GABA-B.
IPR017978. GPCR_3_C.
IPR002456. GPCR_3_GABA_rcpt_B1.
IPR028082. Peripla_BP_I.
IPR000436. Sushi_SCR_CCP.
[Graphical view]
PANTHERiPTHR10519. PTHR10519. 1 hit.
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF00084. Sushi. 2 hits.
[Graphical view]
PRINTSiPR01177. GABAB1RECPTR.
SMARTiSM00032. CCP. 2 hits.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
SSF57535. SSF57535. 2 hits.
PROSITEiPS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
PS50923. SUSHI. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1A (identifier: Q9WV18-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLLLLVPLF LRPLGAGGAQ TPNVTSEGCQ IIHPPWEGGI RYRGLTRDQV
60 70 80 90 100
KAINFLPVDY EIEYVCRGER EVVGPKVRKC LANGSWTDMD TPSRCVRICS
110 120 130 140 150
KSYLTLENGK VFLTGGDLPA LDGARVDFRC DPDFHLVGSS RSICSQGQWS
160 170 180 190 200
TPKPHCQVNR TPHSERRAVY IGALFPMSGG WPGGQACQPA VEMALEDVNS
210 220 230 240 250
RRDILPDYEL KLIHHDSKCD PGQATKYLYE LLYNDPIKII LMPGCSSVST
260 270 280 290 300
LVAEAARMWN LIVLSYGSSS PALSNRQRFP TFFRTHPSAT LHNPTRVKLF
310 320 330 340 350
EKWGWKKIAT IQQTTEVFTS TLDDLEERVK EAGIEITFRQ SFFSDPAVPV
360 370 380 390 400
KNLKRQDARI IVGLFYETEA RKVFCEVYKE RLFGKKYVWF LIGWYADNWF
410 420 430 440 450
KTYDPSINCT VEEMTEAVEG HITTEIVMLN PANTRSISNM TSQEFVEKLT
460 470 480 490 500
KRLKRHPEET GGFQEAPLAY DAIWALALAL NKTSGGGGRS GVRLEDFNYN
510 520 530 540 550
NQTITDQIYR AMNSSSFEGV SGHVVFDASG SRMAWTLIEQ LQGGSYKKIG
560 570 580 590 600
YYDSTKDDLS WSKTDKWIGG SPPADQTLVI KTFRFLSQKL FISVSVLSSL
610 620 630 640 650
GIVLAVVCLS FNIYNSHVRY IQNSQPNLNN LTAVGCSLAL AAVFPLGLDG
660 670 680 690 700
YHIGRSQFPF VCQARLWLLG LGFSLGYGSM FTKIWWVHTV FTKKEEKKEW
710 720 730 740 750
RKTLEPWKLY ATVGLLVGMD ILTLAIWQIV DPLHRTIETF AKEEPKEDID
760 770 780 790 800
VSILPQLEHC SSKKMNTWLG IFYGYKGLLL LLGIFLAYET KSVSTEKIND
810 820 830 840 850
HRAVGMAIYN VAVLCLITAP VTMILSSQQD AAFAFASLAI VFSSYITLVV
860 870 880 890 900
LFVPKMRRLI TRGEWQSEAQ DTMKTGSSTN NNEEEKSRLL EKENRELEKI
910 920 930 940 950
IAEKEERVSE LRHQLQSRQQ IRSRRHPPTP PDPSGGLPRG PSEPPDRLSC
960
DGSRVHLLYK
Length:960
Mass (Da):108,216
Last modified:November 1, 1999 - v1
Checksum:iE4B5A9401E23E8B4
GO
Isoform 1B (identifier: Q9WV18-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-163: MLLLLLVPLF...PHCQVNRTPH → MGPGGPCTPV...PHPRVPPHPS

Show »
Length:844
Mass (Da):95,022
Checksum:i76CA895396EAFDC0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti7 – 82VP → LL in AAD22194. (PubMed:10773016)Curated
Sequence conflicti46 – 461T → I in AAD22194. (PubMed:10773016)Curated
Sequence conflicti618 – 6181V → A in AAD22194. (PubMed:10773016)Curated
Sequence conflicti642 – 6421A → V in AAD22194. (PubMed:10773016)Curated
Sequence conflicti700 – 7001W → R in AAH56990. (PubMed:15489334)Curated
Sequence conflicti721 – 7211I → V in AAD22194. (PubMed:10773016)Curated
Sequence conflicti812 – 8121A → P in AAG29338. (PubMed:11306808)Curated
Sequence conflicti812 – 8121A → P in AAG29341. (PubMed:11306808)Curated
Sequence conflicti869 – 8691A → T in AAD22194. (PubMed:10773016)Curated
Sequence conflicti921 – 9211I → L in AAD22194. (PubMed:10773016)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 163163MLLLL…NRTPH → MGPGGPCTPVGWPLPLLLVM AAGVAPVWASHSPHLPRPHP RVPPHPS in isoform 1B. 2 PublicationsVSP_002041Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF114168 mRNA. Translation: AAD22194.2.
AF008649 mRNA. Translation: AAG29338.1.
AF120255 mRNA. Translation: AAG29341.1.
AL078630 Genomic DNA. No translation available.
BC054735 mRNA. Translation: AAH54735.1.
BC056990 mRNA. Translation: AAH56990.1.
CCDSiCCDS37616.1. [Q9WV18-1]
RefSeqiNP_062312.3. NM_019439.3. [Q9WV18-1]
UniGeneiMm.32191.

Genome annotation databases

EnsembliENSMUST00000025338; ENSMUSP00000025338; ENSMUSG00000024462. [Q9WV18-1]
ENSMUST00000172792; ENSMUSP00000134268; ENSMUSG00000024462. [Q9WV18-2]
GeneIDi54393.
KEGGimmu:54393.
UCSCiuc008cma.1. mouse. [Q9WV18-1]
uc008cmd.1. mouse. [Q9WV18-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF114168 mRNA. Translation: AAD22194.2 .
AF008649 mRNA. Translation: AAG29338.1 .
AF120255 mRNA. Translation: AAG29341.1 .
AL078630 Genomic DNA. No translation available.
BC054735 mRNA. Translation: AAH54735.1 .
BC056990 mRNA. Translation: AAH56990.1 .
CCDSi CCDS37616.1. [Q9WV18-1 ]
RefSeqi NP_062312.3. NM_019439.3. [Q9WV18-1 ]
UniGenei Mm.32191.

3D structure databases

ProteinModelPortali Q9WV18.
SMRi Q9WV18. Positions 54-159, 164-575, 883-917.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 207643. 3 interactions.
IntActi Q9WV18. 3 interactions.
STRINGi 10090.ENSMUSP00000025338.

Chemistry

GuidetoPHARMACOLOGYi 240.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei Q9WV18.

Proteomic databases

MaxQBi Q9WV18.
PaxDbi Q9WV18.
PRIDEi Q9WV18.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000025338 ; ENSMUSP00000025338 ; ENSMUSG00000024462 . [Q9WV18-1 ]
ENSMUST00000172792 ; ENSMUSP00000134268 ; ENSMUSG00000024462 . [Q9WV18-2 ]
GeneIDi 54393.
KEGGi mmu:54393.
UCSCi uc008cma.1. mouse. [Q9WV18-1 ]
uc008cmd.1. mouse. [Q9WV18-2 ]

Organism-specific databases

CTDi 2550.
MGIi MGI:1860139. Gabbr1.

Phylogenomic databases

eggNOGi COG0683.
GeneTreei ENSGT00530000063129.
HOGENOMi HOG000016575.
HOVERGENi HBG051688.
InParanoidi Q9WV18.
KOi K04615.
OMAi STQFLGV.
OrthoDBi EOG7D2FCV.
PhylomeDBi Q9WV18.
TreeFami TF313965.

Enzyme and pathway databases

Reactomei REACT_208837. Activation of G protein gated Potassium channels.
REACT_217868. GABA B receptor activation.
REACT_226457. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.
REACT_250376. G alpha (i) signalling events.
REACT_262510. Class C/3 (Metabotropic glutamate/pheromone receptors).

Miscellaneous databases

ChiTaRSi Gabbr1. mouse.
NextBioi 311240.
PROi Q9WV18.
SOURCEi Search...

Gene expression databases

Bgeei Q9WV18.
CleanExi MM_GABBR1.
ExpressionAtlasi Q9WV18. baseline and differential.
Genevestigatori Q9WV18.

Family and domain databases

InterProi IPR001828. ANF_lig-bd_rcpt.
IPR002455. GPCR3_GABA-B.
IPR017978. GPCR_3_C.
IPR002456. GPCR_3_GABA_rcpt_B1.
IPR028082. Peripla_BP_I.
IPR000436. Sushi_SCR_CCP.
[Graphical view ]
PANTHERi PTHR10519. PTHR10519. 1 hit.
Pfami PF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF00084. Sushi. 2 hits.
[Graphical view ]
PRINTSi PR01177. GABAB1RECPTR.
SMARTi SM00032. CCP. 2 hits.
[Graphical view ]
SUPFAMi SSF53822. SSF53822. 1 hit.
SSF57535. SSF57535. 2 hits.
PROSITEi PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
PS50923. SUSHI. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Coexpression of full-length gamma-aminobutyric acid(B) (GABA(B)) receptors with truncated receptors and metabotropic glutamate receptor 4 supports the GABA(B) heterodimer as the functional receptor."
    Sullivan R., Chateauneuf A., Coulombe N., Kolakowski L.F. Jr., Johnson M.P., Hebert T.E., Ethier N., Belley M., Metters K., Abramovitz M., O'Neill G.P., Ng G.Y.K.
    J. Pharmacol. Exp. Ther. 293:460-467(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1A), FUNCTION, INTERACTION WITH GABBR2, SUBCELLULAR LOCATION.
  2. "The murine GABA(B) receptor 1: cDNA cloning, tissue distribution, structure of the Gabbr1 gene, and mapping to chromosome 17."
    Lamp K., Humeny A., Nikolic Z., Imai K., Adamski J., Schiebel K., Becker C.M.
    Cytogenet. Cell Genet. 92:116-121(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1A AND 1B), TISSUE SPECIFICITY.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1A AND 1B).
    Strain: C57BL/6.
    Tissue: Brain.
  5. "Role of heteromer formation in GABAB receptor function."
    Kuner R., Koehr G., Gruenewald S., Eisenhardt G., Bach A., Kornau H.-C.
    Science 283:74-77(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GABBR2.
  6. Cited for: INTERACTION WITH JAKMIP1.

Entry informationi

Entry nameiGABR1_MOUSE
AccessioniPrimary (citable) accession number: Q9WV18
Secondary accession number(s): Q6PGJ2
, Q9WU48, Q9WV15, Q9WV16, Q9WV17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1999
Last modified: November 26, 2014
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3