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Protein

RNA-binding motif protein, X chromosome

Gene

Rbmx

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding protein that plays several role in the regulation of pre- and post-transcriptional processes. Implicated in tissue-specific regulation of gene transcription and alternative splicing of several pre-mRNAs. Binds to and stimulates transcription from the tumor suppressor TXNIP gene promoter; may thus be involved in tumor suppression. When associated with SAFB, binds to and stimulates transcription from the SREBF1 promoter. Associates with nascent mRNAs transcribed by RNA polymerase II. Component of the supraspliceosome complex that regulates pre-mRNA alternative splice site selection. Can either activate or suppress exon inclusion; acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN2. Represses the splicing of MAPT/Tau exon 10. Binds preferentially to single-stranded 5'-CC[A/C]-rich RNA sequence motifs localized in a single-stranded conformation; probably binds RNA as a homodimer. Binds non-specifically to pre-mRNAs. Plays also a role in the cytoplasmic TNFR1 trafficking pathways; promotes both the IL-1-beta-mediated inducible proteolytic cleavage of TNFR1 ectodomains and the release of TNFR1 exosome-like vesicles to the extracellular compartment.2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor, Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing, Transcription

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-binding motif protein, X chromosome
Alternative name(s):
Heterogeneous nuclear ribonucleoprotein G
Short name:
hnRNP G
Cleaved into the following chain:
Gene namesi
Name:Rbmx
Synonyms:Hnrnpg, Hnrpg, Rbmxp1, Rbmxrt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1343044. Rbmx.

Subcellular locationi

  • Nucleus

  • Note: Localizes in numerous small granules in the nucleus (By similarity). Component of ribonucleosomes.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004147471 – 391RNA-binding motif protein, X chromosomeAdd BLAST391
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00004147482 – 391RNA-binding motif protein, X chromosome, N-terminally processedAdd BLAST390

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylvaline; in RNA-binding motif protein, X chromosome, N-terminally processedBy similarity1
Modified residuei30N6-acetyllysineBy similarity1
Cross-linki80Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei88PhosphoserineBy similarity1
Modified residuei91PhosphoserineBy similarity1
Modified residuei125Asymmetric dimethylarginine; alternateBy similarity1
Modified residuei125Omega-N-methylarginine; alternateCombined sources1
Modified residuei144Omega-N-methylarginineCombined sources1
Modified residuei164Omega-N-methylarginineCombined sources1
Modified residuei165PhosphoserineBy similarity1
Modified residuei172Omega-N-methylarginineCombined sources1
Modified residuei174PhosphoserineBy similarity1
Modified residuei185Omega-N-methylated arginineBy similarity1
Modified residuei261PhosphoserineBy similarity1
Modified residuei328PhosphoserineBy similarity1
Modified residuei329PhosphoserineBy similarity1
Modified residuei330PhosphoserineBy similarity1
Modified residuei332PhosphoserineBy similarity1
Modified residuei352PhosphoserineBy similarity1

Post-translational modificationi

O-glycosylated.By similarity
Arg-182 is dimethylated, probably to asymmetric dimethylarginine.

Keywords - PTMi

Acetylation, Glycoprotein, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9WV02.
PaxDbiQ9WV02.
PeptideAtlasiQ9WV02.
PRIDEiQ9WV02.
TopDownProteomicsiQ9WV02-1. [Q9WV02-1]

PTM databases

iPTMnetiQ9WV02.
PhosphoSitePlusiQ9WV02.

Expressioni

Tissue specificityi

Both isoforms are widely expressed.1 Publication

Inductioni

By high-fructose diet.1 Publication

Gene expression databases

BgeeiENSMUSG00000031134.
ExpressionAtlasiQ9WV02. baseline and differential.
GenevisibleiQ9WV02. MM.

Interactioni

Subunit structurei

Homomultimer. Found in the supraspliceosome complex. Identified in the spliceosome C complex. Interacts with KHDRBS3. Forms a complex with ILF2, ILF3, YLPM1, KHDRBS1, NCOA5 and PPP1CA Interacts with CLK2, KHDRBS2, SAFB, TRA2B and YTHDC1. Interacts with ERAP1; the interaction is RNA-independent (By similarity). Interacts with SAFB/SAFB1.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202824. 2 interactors.
IntActiQ9WV02. 3 interactors.
MINTiMINT-4131116.
STRINGi10090.ENSMUSP00000110374.

Structurei

3D structure databases

ProteinModelPortaliQ9WV02.
SMRiQ9WV02.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 86RRMPROSITE-ProRule annotationAdd BLAST79

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi104 – 192Gly-richAdd BLAST89
Compositional biasi249 – 337Ser-richAdd BLAST89

Domaini

The RRM domain is necessary for RNA-binding, but not for splice site selection, indicating that its splicing activity does not require direct binding to RNA.By similarity

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IHG3. Eukaryota.
ENOG4111GI6. LUCA.
GeneTreeiENSGT00710000106295.
HOGENOMiHOG000276235.
HOVERGENiHBG063314.
InParanoidiQ9WV02.
KOiK12885.
OMAiRDYSYRE.
OrthoDBiEOG091G13K0.
PhylomeDBiQ9WV02.
TreeFamiTF331833.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR012604. RBM1CTR.
IPR000504. RRM_dom.
IPR003954. RRM_dom_euk.
[Graphical view]
PfamiPF08081. RBM1CTR. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00361. RRM_1. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9WV02-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVEADRPGKL FIGGLNTETN EKALEAVFGK YGRIVEVLLM KDRETNKSRG
60 70 80 90 100
FAFVTFESPA DAKDAARDMN GKSLDGKAIK VEQATKPSFE SGRRGLPPPP
110 120 130 140 150
RSRGPPRGLR GGRGGSGGTR GPPSRGGHMD DGGYSMNFTM SSSRGPLPVK
160 170 180 190 200
RGPPPRSGGP PPKRSAPSGP VRSSSGLGGR APVSRGRDGY GGPPRREPLP
210 220 230 240 250
SRRDVYLSPR DDGYSTKDSY SSREYPSSRD TRDYAPPPRD YTYRDYGHSS
260 270 280 290 300
SRDDYPSRGY SDRDGYGRDR DYSDHPSGGS YRDSYESYGN SRSAPPTRGP
310 320 330 340 350
PPSYGGSSRY DDYSSSRDGY GGSRDSYSSS RSDLYSSGRD RVGRQERGLP
360 370 380 390
PSMERGYPPP RDSYSSSSRG APRGGGRGGS RSDRGGGRSR Y
Length:391
Mass (Da):42,301
Last modified:November 1, 1999 - v1
Checksum:i301E02E250EDDCD3
GO
Isoform 2 (identifier: Q9WV02-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     290-391: Missing.

Show »
Length:289
Mass (Da):31,414
Checksum:i530B4B1EBF286AFD
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_042129290 – 391Missing in isoform 2. 2 PublicationsAdd BLAST102

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ237846 mRNA. Translation: CAB51361.1.
AJ237847 mRNA. Translation: CAB51362.1.
AK087940 mRNA. Translation: BAC40051.1.
AK135164 mRNA. Translation: BAE22448.1.
AK165006 mRNA. Translation: BAE37998.1.
AL672106 Genomic DNA. Translation: CAM22440.1.
CH466583 Genomic DNA. Translation: EDL42174.1.
CH466583 Genomic DNA. Translation: EDL42172.1.
BC003710 mRNA. Translation: AAH03710.1.
CCDSiCCDS30153.1. [Q9WV02-1]
RefSeqiNP_001160095.1. NM_001166623.1. [Q9WV02-1]
NP_035382.1. NM_011252.4. [Q9WV02-1]
UniGeneiMm.28275.

Genome annotation databases

EnsembliENSMUST00000114726; ENSMUSP00000110374; ENSMUSG00000031134. [Q9WV02-1]
ENSMUST00000114730; ENSMUSP00000110378; ENSMUSG00000031134. [Q9WV02-1]
ENSMUST00000140384; ENSMUSP00000125720; ENSMUSG00000031134. [Q9WV02-2]
GeneIDi19655.
KEGGimmu:19655.
UCSCiuc009thi.2. mouse. [Q9WV02-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ237846 mRNA. Translation: CAB51361.1.
AJ237847 mRNA. Translation: CAB51362.1.
AK087940 mRNA. Translation: BAC40051.1.
AK135164 mRNA. Translation: BAE22448.1.
AK165006 mRNA. Translation: BAE37998.1.
AL672106 Genomic DNA. Translation: CAM22440.1.
CH466583 Genomic DNA. Translation: EDL42174.1.
CH466583 Genomic DNA. Translation: EDL42172.1.
BC003710 mRNA. Translation: AAH03710.1.
CCDSiCCDS30153.1. [Q9WV02-1]
RefSeqiNP_001160095.1. NM_001166623.1. [Q9WV02-1]
NP_035382.1. NM_011252.4. [Q9WV02-1]
UniGeneiMm.28275.

3D structure databases

ProteinModelPortaliQ9WV02.
SMRiQ9WV02.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202824. 2 interactors.
IntActiQ9WV02. 3 interactors.
MINTiMINT-4131116.
STRINGi10090.ENSMUSP00000110374.

PTM databases

iPTMnetiQ9WV02.
PhosphoSitePlusiQ9WV02.

Proteomic databases

EPDiQ9WV02.
PaxDbiQ9WV02.
PeptideAtlasiQ9WV02.
PRIDEiQ9WV02.
TopDownProteomicsiQ9WV02-1. [Q9WV02-1]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000114726; ENSMUSP00000110374; ENSMUSG00000031134. [Q9WV02-1]
ENSMUST00000114730; ENSMUSP00000110378; ENSMUSG00000031134. [Q9WV02-1]
ENSMUST00000140384; ENSMUSP00000125720; ENSMUSG00000031134. [Q9WV02-2]
GeneIDi19655.
KEGGimmu:19655.
UCSCiuc009thi.2. mouse. [Q9WV02-1]

Organism-specific databases

CTDi27316.
MGIiMGI:1343044. Rbmx.

Phylogenomic databases

eggNOGiENOG410IHG3. Eukaryota.
ENOG4111GI6. LUCA.
GeneTreeiENSGT00710000106295.
HOGENOMiHOG000276235.
HOVERGENiHBG063314.
InParanoidiQ9WV02.
KOiK12885.
OMAiRDYSYRE.
OrthoDBiEOG091G13K0.
PhylomeDBiQ9WV02.
TreeFamiTF331833.

Miscellaneous databases

ChiTaRSiRbmx. mouse.
PROiQ9WV02.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031134.
ExpressionAtlasiQ9WV02. baseline and differential.
GenevisibleiQ9WV02. MM.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR012604. RBM1CTR.
IPR000504. RRM_dom.
IPR003954. RRM_dom_euk.
[Graphical view]
PfamiPF08081. RBM1CTR. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00361. RRM_1. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRBMX_MOUSE
AccessioniPrimary (citable) accession number: Q9WV02
Secondary accession number(s): Q8C2U6, Q9R0Y0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: November 1, 1999
Last modified: November 30, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.