Reviewed, UniProtKB/Swiss-Prot Q9WUX6 (RNAS1_URARU)

Names and origin

Protein names

Recommended name:
    Ribonuclease pancreatic
    EC=3.1.27.5
Alternative name(s):
    RNase 1
    RNase A

Gene names

Name:

RNASE1

Organism

Uranomys ruddi (White-bellied brush-furred rat)

Taxonomic identifier

41272 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Deomyinae › Uranomys

Protein attributes

Sequence length	149 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA By similarity.
Catalytic activity	Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.
Subunit structure	Monomer By similarity.
Subcellular location	Secreted.
Tissue specificity	Pancreas.
Sequence similarities	Belongs to the pancreatic ribonuclease family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro pancreatic ribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	By similarity
	Chain	26 – 149	124	Ribonuclease pancreatic		PRO_0000030945
Regions
	Region	66 – 70	5	Substrate binding By similarity
Sites
	Active site	37	1	Proton acceptor By similarity
	Active site	144	1	Proton donor By similarity
	Binding site	32	1	Substrate By similarity
	Binding site	35	1	Substrate By similarity
	Binding site	91	1	Substrate By similarity
	Binding site	110	1	Substrate By similarity
Amino acid modifications
	Disulfide bond	51 ↔ 109		By similarity
	Disulfide bond	65 ↔ 120		By similarity
	Disulfide bond	83 ↔ 135		By similarity
	Disulfide bond	90 ↔ 97		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9WUX6-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 0531207ECBF5C1EE	FASTA	149	16,798
	10 20 30 40 50 60 MGLEKSLILF PLLVLVLGWV QPSLARESPA MKFERQHMDS DGASSSSSTY CNQMMKRREM 70 80 90 100 110 120 TKESCKPVNT FVHEPLADVQ AVCSQENVTC KNGKQNCYKS TSALHITDCR LKDNSKYPNC 130 140 DYKTSQYQKH IIVACEGNPY VPVHFDATV

References

[1]

"The phylogenetic position of 'Acomyinae' (Rodentia, Mammalia) as sister group of a Murinae + Gerbillinae clade: evidence from the nuclear ribonuclease gene." Dubois J.-Y.F., Catzeflis F.M., Beintema J.J. Mol. Phylogenet. Evol. 13:181-192(1999) [PubMed: 10508551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ005773 Genomic DNA. Translation: CAB41486.1.
3D structure databases
HSSP	HSSP built from PDB template 1SRN based on UniProtKB P00656.
SMR	Q9WUX6. Positions 27-149.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q9WUX6.
Enzyme and pathway databases
BRENDA	3.1.27.5. 315251.
Family and domain databases
InterPro	IPR001427. RNaseA. [Graphical view]
Gene3D	G3DSA:3.10.130.10. RNaseA. 1 hit.
PANTHER	PTHR11437. RNaseA. 1 hit.
Pfam	PF00074. RnaseA. 1 hit. [Graphical view]
PRINTS	PR00794. RIBONUCLEASE.
ProDom	PD000535. RNaseA. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00092. RNAse_Pc. 1 hit. [Graphical view]
PROSITE	PS00127. RNASE_PANCREATIC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNAS1_URARU

Accession

Primary (citable) accession number: Q9WUX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	November 1, 1999
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)