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Protein

Complement factor I

Gene

Cfi

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Responsible for cleaving the alpha-chains of C4b and C3b in the presence of the cofactors C4-binding protein and factor H respectively.

Catalytic activityi

Inactivates complement subcomponents C3b, iC3b and C4b by proteolytic cleavage.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei402 – 4021Charge relay systemBy similarity
Active sitei450 – 4501Charge relay systemBy similarity
Active sitei546 – 5461Charge relay systemBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi244 – 25815By similarityAdd
BLAST
Calcium bindingi281 – 29515By similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Complement pathway, Immunity, Innate immunity

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

MEROPSiS01.199.

Names & Taxonomyi

Protein namesi
Recommended name:
Complement factor I (EC:3.4.21.45)
Alternative name(s):
C3B/C4B inactivator
Cleaved into the following 2 chains:
Gene namesi
Name:Cfi
Synonyms:If
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620429. Cfi.

Subcellular locationi

GO - Cellular componenti

  • extracellular space Source: RGD
  • membrane Source: InterPro
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence analysisAdd
BLAST
Chaini19 – 604586Complement factor IPRO_0000027574Add
BLAST
Chaini19 – 357339Complement factor I heavy chainPRO_0000027575Add
BLAST
Chaini362 – 604243Complement factor I light chainPRO_0000027576Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi36 ↔ 260By similarity
Glycosylationi40 – 401N-linked (GlcNAc...)Sequence analysis
Disulfide bondi46 ↔ 57By similarity
Disulfide bondi51 ↔ 62By similarity
Disulfide bondi64 ↔ 96By similarity
Disulfide bondi70 ↔ 89By similarity
Disulfide bondi78 ↔ 109By similarity
Glycosylationi106 – 1061N-linked (GlcNAc...)Sequence analysis
Glycosylationi116 – 1161N-linked (GlcNAc...)Sequence analysis
Disulfide bondi144 ↔ 186By similarity
Disulfide bondi157 ↔ 219By similarity
Glycosylationi182 – 1821N-linked (GlcNAc...)Sequence analysis
Disulfide bondi191 ↔ 201By similarity
Disulfide bondi234 ↔ 252By similarity
Disulfide bondi264 ↔ 276By similarity
Disulfide bondi271 ↔ 289By similarity
Disulfide bondi283 ↔ 298By similarity
Disulfide bondi349 ↔ 474Interchain (between heavy and light chains)PROSITE-ProRule annotation
Disulfide bondi387 ↔ 403By similarity
Disulfide bondi395 ↔ 465By similarity
Disulfide bondi488 ↔ 552By similarity
Glycosylationi515 – 5151N-linked (GlcNAc...)Sequence analysis
Disulfide bondi516 ↔ 531By similarity
Disulfide bondi542 ↔ 571By similarity
Glycosylationi557 – 5571N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9WUW3.
PRIDEiQ9WUW3.

PTM databases

iPTMnetiQ9WUW3.
PhosphoSiteiQ9WUW3.

Expressioni

Tissue specificityi

Plasma.

Gene expression databases

BgeeiENSRNOG00000023599.

Interactioni

Subunit structurei

Heterodimer of a light and heavy chains; disulfide-linked. The fully processed and mature protein circulates as a zymogen, and is allosterically activated by substrate-induced remodeling of the active site (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000032881.

Structurei

3D structure databases

ProteinModelPortaliQ9WUW3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini58 – 11154Kazal-likePROSITE-ProRule annotationAdd
BLAST
Domaini117 – 217101SRCRPROSITE-ProRule annotationAdd
BLAST
Domaini218 – 26245LDL-receptor class A 1PROSITE-ProRule annotationAdd
BLAST
Domaini263 – 29937LDL-receptor class A 2PROSITE-ProRule annotationAdd
BLAST
Domaini362 – 595234Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 Kazal-like domain.PROSITE-ProRule annotation
Contains 2 LDL-receptor class A domains.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation
Contains 1 SRCR domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
HOVERGENiHBG005311.
InParanoidiQ9WUW3.
KOiK01333.
PhylomeDBiQ9WUW3.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
Gene3Di3.10.250.10. 1 hit.
4.10.400.10. 2 hits.
InterProiIPR003884. FacI_MAC.
IPR002350. Kazal_dom.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001190. SRCR.
IPR017448. SRCR-like_dom.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF07648. Kazal_2. 1 hit.
PF00057. Ldl_recept_a. 2 hits.
PF00530. SRCR. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00057. FIMAC. 1 hit.
SM00280. KAZAL. 1 hit.
SM00192. LDLa. 2 hits.
SM00202. SR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF56487. SSF56487. 1 hit.
SSF57424. SSF57424. 2 hits.
PROSITEiPS51465. KAZAL_2. 1 hit.
PS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 2 hits.
PS50287. SRCR_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9WUW3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLALLILLL LNPHLSSSKN TPASGQPQED LVEQKCLLKN YTHHSCDKVF
60 70 80 90 100
CQPWQKCIEG TCACKLPYQC PKAGTPVCAT NGRGYPTYCH LKSFECLHPE
110 120 130 140 150
IKFSNNGTCT AEEKFNVSLI YGSTDTEGIV QVKLVDQDEK MFICKNSWST
160 170 180 190 200
VEANVACFDL GFPLGVRDIQ GRFNIPVNHK INSTECLHVR CQGVETSLAE
210 220 230 240 250
CTFTKKSSKA PHGLAGVVCY TQDADFPTSQ SFQCVNGKRI PQEKACDGVN
260 270 280 290 300
DCGDQSDELC CKGCRGQAFL CKSGVCIPNQ RKCNGEVDCI TGEDESGCEE
310 320 330 340 350
DKKNKIHKGL ARSDQGGETE IETEETEMLT PDMDTERKRI KSLLPKLSCG
360 370 380 390 400
VKRNTHIRRK RVVGGKPAEM GDYPWQVAIK DGDRITCGGI YIGGCWILTA
410 420 430 440 450
AHCVRPSRYR NYQVWTSLLD WLKPNSQLAV QGVSRVVVHE KYNGATYQND
460 470 480 490 500
IALVEMKKHP GKKECELINS VPACVPWSPY LFQPNDRCII SGWGREKDNQ
510 520 530 540 550
KVYSLRWGEV DLIGNCSRFY PGRYYEKEMQ CAGTSDGSID ACKGDSGGPL
560 570 580 590 600
VCKDVNNVTY VWGIVSWGEN CGKPEFPGVY TRVASYFDWI SYYVGRPLVS

QYNV
Length:604
Mass (Da):67,298
Last modified:November 1, 1999 - v1
Checksum:iC775AE68D2D52D51
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y18965 mRNA. Translation: CAB41688.1.
BC089798 mRNA. Translation: AAH89798.1.
RefSeqiNP_077071.1. NM_024157.1.
UniGeneiRn.7424.

Genome annotation databases

GeneIDi79126.
KEGGirno:79126.
UCSCiRGD:620429. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y18965 mRNA. Translation: CAB41688.1.
BC089798 mRNA. Translation: AAH89798.1.
RefSeqiNP_077071.1. NM_024157.1.
UniGeneiRn.7424.

3D structure databases

ProteinModelPortaliQ9WUW3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000032881.

Protein family/group databases

MEROPSiS01.199.

PTM databases

iPTMnetiQ9WUW3.
PhosphoSiteiQ9WUW3.

Proteomic databases

PaxDbiQ9WUW3.
PRIDEiQ9WUW3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi79126.
KEGGirno:79126.
UCSCiRGD:620429. rat.

Organism-specific databases

CTDi3426.
RGDi620429. Cfi.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
HOVERGENiHBG005311.
InParanoidiQ9WUW3.
KOiK01333.
PhylomeDBiQ9WUW3.

Miscellaneous databases

PROiQ9WUW3.

Gene expression databases

BgeeiENSRNOG00000023599.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
Gene3Di3.10.250.10. 1 hit.
4.10.400.10. 2 hits.
InterProiIPR003884. FacI_MAC.
IPR002350. Kazal_dom.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001190. SRCR.
IPR017448. SRCR-like_dom.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF07648. Kazal_2. 1 hit.
PF00057. Ldl_recept_a. 2 hits.
PF00530. SRCR. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00057. FIMAC. 1 hit.
SM00280. KAZAL. 1 hit.
SM00192. LDLa. 2 hits.
SM00202. SR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF56487. SSF56487. 1 hit.
SSF57424. SSF57424. 2 hits.
PROSITEiPS51465. KAZAL_2. 1 hit.
PS01209. LDLRA_1. 1 hit.
PS50068. LDLRA_2. 2 hits.
PS50287. SRCR_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCFAI_RAT
AccessioniPrimary (citable) accession number: Q9WUW3
Secondary accession number(s): Q5EBC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: November 1, 1999
Last modified: September 7, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.