ID   ORC5_MOUSE              Reviewed;         435 AA.
AC   Q9WUV0; Q3TMI6;
DT   21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   24-JAN-2024, entry version 149.
DE   RecName: Full=Origin recognition complex subunit 5;
GN   Name=Orc5; Synonyms=Orc5l;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=10460412; DOI=10.1007/s004120050374;
RA   Springer J., Kneissl M., Putter V., Grummt F.;
RT   "Identification and characterization of MmORC4 and MmORC5, two subunits of
RT   the mouse origin of replication recognition complex.";
RL   Chromosoma 108:243-249(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Heart, Lung, and Mammary gland;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=FVB/N; TISSUE=Mammary gland;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Component of the origin recognition complex (ORC) that binds
CC       origins of replication. DNA-binding is ATP-dependent. The specific DNA
CC       sequences that define origins of replication have not been identified
CC       yet. ORC is required to assemble the pre-replication complex necessary
CC       to initiate DNA replication. {ECO:0000250|UniProtKB:O43913}.
CC   -!- SUBUNIT: Component of ORC, a complex composed of at least 6 subunits:
CC       ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle
CC       dependent manner. It is sequentially assembled at the exit from
CC       anaphase of mitosis and disassembled as cells enter S phase.
CC       {ECO:0000250|UniProtKB:O43913}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O43913}.
CC       Chromosome {ECO:0000250|UniProtKB:O43913}.
CC   -!- PTM: Multi-mono-ubiquitinated by OBI1; ubiquitination is important for
CC       efficient DNA replication origin site activation. Ubiquitination levels
CC       are low in mitotic and early G1-phAse cells and are induced in late
CC       G1-/early S-phase, peaking in S-phase and decrease toward the end of
CC       the cell cycle. {ECO:0000250|UniProtKB:O43913}.
CC   -!- SIMILARITY: Belongs to the ORC5 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AJ007360; CAB43767.1; -; mRNA.
DR   EMBL; AK084683; BAC39250.1; -; mRNA.
DR   EMBL; AK144983; BAE26167.1; -; mRNA.
DR   EMBL; AK165912; BAE38456.1; -; mRNA.
DR   EMBL; AK166537; BAE38839.1; -; mRNA.
DR   EMBL; BC006927; AAH06927.1; -; mRNA.
DR   CCDS; CCDS19110.1; -.
DR   RefSeq; NP_036089.1; NM_011959.2.
DR   AlphaFoldDB; Q9WUV0; -.
DR   SMR; Q9WUV0; -.
DR   BioGRID; 204981; 2.
DR   ComplexPortal; CPX-1915; Nuclear origin recognition complex.
DR   CORUM; Q9WUV0; -.
DR   IntAct; Q9WUV0; 2.
DR   MINT; Q9WUV0; -.
DR   STRING; 10090.ENSMUSP00000030872; -.
DR   iPTMnet; Q9WUV0; -.
DR   PhosphoSitePlus; Q9WUV0; -.
DR   EPD; Q9WUV0; -.
DR   MaxQB; Q9WUV0; -.
DR   PaxDb; 10090-ENSMUSP00000030872; -.
DR   PeptideAtlas; Q9WUV0; -.
DR   ProteomicsDB; 293522; -.
DR   Pumba; Q9WUV0; -.
DR   Antibodypedia; 31233; 119 antibodies from 23 providers.
DR   DNASU; 26429; -.
DR   Ensembl; ENSMUST00000030872.12; ENSMUSP00000030872.6; ENSMUSG00000029012.12.
DR   GeneID; 26429; -.
DR   KEGG; mmu:26429; -.
DR   UCSC; uc008wpq.1; mouse.
DR   AGR; MGI:1347044; -.
DR   CTD; 5001; -.
DR   MGI; MGI:1347044; Orc5.
DR   VEuPathDB; HostDB:ENSMUSG00000029012; -.
DR   eggNOG; KOG2543; Eukaryota.
DR   GeneTree; ENSGT00390000009380; -.
DR   HOGENOM; CLU_028223_0_0_1; -.
DR   InParanoid; Q9WUV0; -.
DR   OMA; QLRRWHG; -.
DR   OrthoDB; 469431at2759; -.
DR   PhylomeDB; Q9WUV0; -.
DR   TreeFam; TF101095; -.
DR   Reactome; R-MMU-176187; Activation of ATR in response to replication stress.
DR   Reactome; R-MMU-68616; Assembly of the ORC complex at the origin of replication.
DR   Reactome; R-MMU-68689; CDC6 association with the ORC:origin complex.
DR   Reactome; R-MMU-68949; Orc1 removal from chromatin.
DR   Reactome; R-MMU-68962; Activation of the pre-replicative complex.
DR   BioGRID-ORCS; 26429; 26 hits in 77 CRISPR screens.
DR   ChiTaRS; Orc5; mouse.
DR   PRO; PR:Q9WUV0; -.
DR   Proteomes; UP000000589; Chromosome 5.
DR   RNAct; Q9WUV0; Protein.
DR   Bgee; ENSMUSG00000029012; Expressed in primary oocyte and 285 other cell types or tissues.
DR   ExpressionAtlas; Q9WUV0; baseline and differential.
DR   GO; GO:0000785; C:chromatin; ISS:UniProtKB.
DR   GO; GO:0005829; C:cytosol; ISO:MGI.
DR   GO; GO:0005664; C:nuclear origin of replication recognition complex; ISS:UniProtKB.
DR   GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR   GO; GO:0000808; C:origin recognition complex; ISO:MGI.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003688; F:DNA replication origin binding; IBA:GO_Central.
DR   GO; GO:0006260; P:DNA replication; TAS:MGI.
DR   GO; GO:0006270; P:DNA replication initiation; ISO:MGI.
DR   GO; GO:0006275; P:regulation of DNA replication; ISS:UniProtKB.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   InterPro; IPR041664; AAA_16.
DR   InterPro; IPR020796; ORC5.
DR   InterPro; IPR047088; ORC5_C.
DR   InterPro; IPR048866; ORC5_lid.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR12705; ORIGIN RECOGNITION COMPLEX SUBUNIT 5; 1.
DR   PANTHER; PTHR12705:SF0; ORIGIN RECOGNITION COMPLEX SUBUNIT 5; 1.
DR   Pfam; PF13191; AAA_16; 1.
DR   Pfam; PF14630; ORC5_C; 1.
DR   Pfam; PF21639; ORC5_lid; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   Genevisible; Q9WUV0; MM.
PE   2: Evidence at transcript level;
KW   ATP-binding; Chromosome; DNA replication; Nucleotide-binding; Nucleus;
KW   Reference proteome; Ubl conjugation.
FT   CHAIN           1..435
FT                   /note="Origin recognition complex subunit 5"
FT                   /id="PRO_0000127093"
FT   BINDING         37..44
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   435 AA;  50217 MW;  1F334E35379F5C69 CRC64;
     MSHLESMVLC REAQVSTLQS LFGERHHFSF PSIFIYGHTA SGKTYVTQTL LKTLELPHAF
     VNCVECFTSR LLLEQILNKL SHLNSSDAGC STEMTCETFN DFVQLFKQVT SAEHLKDQTV
     YIVLDKAEYL RDMEANLLPG LLRLQELTDR NVTVIFLSEI IWEKFRPNTG CFEPFVLYFP
     DYSIGNLQKI LSHDHPPEYS ADFYAAYINI LLGVFYTVCR DLKELRHLAV LNFPKYCEPV
     VKGEAGERDT RKLWRNIEPH LKKAMQTVYL REISSSQWEK LQKDNTDPGQ LKGLSAYTHM
     ELPYYSKFIL IAAYLASYNP ARTDKRFFLK HHGKIKKTNF LKKHEKTSNH LLGPKPFPLD
     RLLAILYSIV DSRVAPTANI FSQITSLVTL QLLTIVGHED QLNGPKYKCT VSLDFIRAIA
     RMVNFDIIKY LYDFL
//