SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9WUU9

- GANP_MOUSE

UniProt

Q9WUU9 - GANP_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Germinal-center associated nuclear protein

Gene
Mcm3ap, Ganp, Map80
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the nuclear export of poly(A)-containing mRNAs by acting as a scaffold for the TREX-2 complex. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery By similarity. Essential for the generation of high-affinity B-cells against T-cell-dependent antigens by affecting somatic hypermutation at the IgV-regions. May have stimulation-dependent DNA primase activity that would generate extra RNA primers in very rapidely proliferating cells and would support clonal expansion of differentiating B-cells.2 Publications

GO - Biological processi

  1. immune system process Source: UniProtKB-KW
  2. mRNA transport Source: UniProtKB-KW
  3. protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Immunity, mRNA transport, Protein transport, Translocation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Germinal-center associated nuclear protein
Short name:
GANP
Alternative name(s):
GC-associated DNA primase
Gene namesi
Name:Mcm3ap
Synonyms:Ganp, Map80
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:1930089. Mcm3ap.

Subcellular locationi

Cytoplasm. Nucleus. Nucleusnuclear pore complex By similarity
Note: In B-cells nuclear import depends on interaction with AICDA/AID, and GANP is selectively targeted to the IgV-region gene.1 Publication

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. cytosol Source: Ensembl
  3. nuclear pore Source: UniProtKB-SubCell
  4. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nuclear pore complex, Nucleus

Pathology & Biotechi

Disruption phenotypei

Causes embryonic death at E12.5 with multiple organ impairments.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi502 – 5021S → A: Loss of DNA primase activity. 1 Publication
Mutagenesisi502 – 5021S → D: Higher DNA primase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 19711971Germinal-center associated nuclear proteinPRO_0000096285Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei483 – 4831N6-acetyllysine1 Publication
Modified residuei484 – 4841N6-acetyllysine1 Publication
Modified residuei502 – 5021Phosphoserine1 Publication
Modified residuei531 – 5311Phosphoserine By similarity

Post-translational modificationi

Phosphorylation at Ser-502 by CDKs is required for DNA primase activity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9WUU9.
PRIDEiQ9WUU9.

PTM databases

PhosphoSiteiQ9WUU9.

Expressioni

Tissue specificityi

Ubiquitous, up-regulated in B-cells germinal centers during T-cell-dependent immune response.2 Publications

Gene expression databases

BgeeiQ9WUU9.
GenevestigatoriQ9WUU9.

Interactioni

Subunit structurei

Component of the nuclear pore complex (NPC)-associated TREX-2 complex (transcription and export complex 2), composed of at least GANP, two copies of ENY2, PCID2, DSS1, and either centrin CETN2 or CETN3. Interacts with NXF1 (By via FG-repeats) By similarity. Interacts with MCM3. Interacts with AICDA/AID.2 Publications

Protein-protein interaction databases

IntActiQ9WUU9. 1 interaction.
MINTiMINT-4101590.

Structurei

3D structure databases

ProteinModelPortaliQ9WUU9.
SMRiQ9WUU9. Positions 1159-1227.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni33 – 335303FG-repeatsAdd
BLAST
Regioni414 – 550137DNA primaseAdd
BLAST
Regioni629 – 983355SAC3 homologyAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1124 – 116239 Reviewed predictionAdd
BLAST

Sequence similaritiesi

Belongs to the SAC3 family.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5079.
GeneTreeiENSGT00530000063781.
HOVERGENiHBG052431.
InParanoidiQ7TS87.
OMAiAHQMKVQ.
OrthoDBiEOG78D7JB.
TreeFamiTF105948.

Family and domain databases

InterProiIPR005062. SAC3/GANP/Nin1/mts3/eIF-3-p25.
[Graphical view]
PfamiPF03399. SAC3_GANP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WUU9-1 [UniParc]FASTAAdd to Basket

« Hide

MHPVNPFGGQ QPSAFAVSSS TTGTYQTKSP FRFGQPSLFG QNSTPSKSLA     50
FSQVPSFATP SGGSHSSSLP AFGLTQTSSV GLFSSLESTP SFAATSSSSV 100
PGNTAFSFKS TSSVGVFPSG ATFGPETGEV AGSGFRKTEF KFKPLENAVF 150
KPIPGPESEP EKTQSQISSG FFTFSHPVGS GSGGLTPFSF PQVTNSSVTS 200
SSFIFSKPVT SNTPAFASPL SNQNVEEEKR VSTSAFGSSN SSFSTFPTAS 250
PGSLGEPFPA NKPSLRQGCE EAISQVEPLP TLMKGLKRKE DQDRSPRRHC 300
HEAAEDPDPL SRGDHPPDKR PVRLNRPRGG TLFGRTIQEV FKSNKEAGRL 350
GSKESKESGF AEPGESDHAA VPGGSQSTMV PSRLPAVTKE EEESRDEKED 400
SLRGKSVRQS KRREEWIYSL GGVSSLELTA IQCKNIPDYL NDRAILEKHF 450
SKIAKVQRVF TRRSKKLAVI HFFDHASAAL ARKKGKGLHK DVVIFWHKKK 500
ISPSKKLFPL KEKLGESEAS QGIEDSPFQH SPLSKPIVRP AAGSLLSKSS 550
PVKKPSLLKM HQFEADPFDS GSEGSEGLGS CVSSLSTLIG TVADTSEEKY 600
RLLDQRDRIM RQARVKRTDL DKARAFVGTC PDMCPEKERY LRETRSQLSV 650
FEVVPGTDQV DHAAAVKEYS RSSADQEEPL PHELRPSAVL SRTMDYLVTQ 700
IMDQKEGSLR DWYDFVWNRT RGIRKDITQQ HLCDPLTVSL IEKCTRFHIH 750
CAHFMCEEPM SSFDAKINNE NMTKCLQSLK EMYQDLRNKG VFCASEAEFQ 800
GYNVLLNLNK GDILREVQQF HPDVRNSPEV NFAVQAFAAL NSNNFVRFFK 850
LVQSASYLNA CLLHCYFNQI RKDALRALNV AYTVSTQRST VFPLDGVVRM 900
LLFRDSEEAT NFLNYHGLTV ADGCVELNRS AFLEPEGLCK ARKSVFIGRK 950
LTVSVGEVVN GGPLPPVPRH TPVCSFNSQN KYVGESLATE LPISTQRAGG 1000
DPAGGGRGED CEAEVDVPTL AVLPQPPPAS SATPALHVQP LAPAAAPSLL 1050
QASTQPEVLL PKPAPVYSDS DLVQVVDELI QEALQVDCEE VSSAGAAYVA 1100
AALGVSNAAV EDLITAATTG ILRHVAAEEV SMERQRLEEE KQRAEEERLK 1150
QERELMLTQL SEGLAAELTE LTVTECVWET CSQELQSAVE IDQKVRVARC 1200
CEAVCAHLVD LFLAEEIFQT AKETLQELQC FCKYLQRWRE AVAARKKFRR 1250
QMRAFPAAPC CVDVNDRLQA LVPSAECPIT EENLAKGLLD LGHAGKVGVS 1300
CTRLRRLRNK TAHQIKVQHF HQQLLRNAAW APLDLPSIVS EHLPMKQKRR 1350
FWKLVLVLPD VEEQTPESPG RILENWLKVK FTGDDSMVGD IGDNAGDIQT 1400
LSVFNTLSSK GDQTVSVNVC IKVAHGTLSD SALDAVETQK DLLGTSGLML 1450
LLPPKVKSEE VAEEELSWLS ALLQLKQLLQ AKPFQPALPL VVLVPSSRGD 1500
SAGRAVEDGL MLQDLVSAKL ISDYIVVEIP DSVNDLQGTV KVSGAVQWLI 1550
SRCPQALDLC CQTLVQYVED GISREFSRRF FHDRRERRLA SLPSQEPSTI 1600
IELFNSVLQF LASVVSSEQL CDISWPVMEF AEVGGSQLLP HLHWNSPEHL 1650
AWLKQAVLGF QLPQMDLPPP GAPWLPVCSM VIQYTSQIPS SSQTQPVLQS 1700
QVENLLCRTY QKWKNKSLSP GQELGPSVAE IPWDDIITLC INHKLRDWTP 1750
PRLPVTLEAL SEDGQICVYF FKNLLRKYHV PLSWEQARMQ TQRELQLSHG 1800
RSGMRSIHPP TSTFPTPLLH VHQKGKKKEE SGREGSLSTE DLLRGASAEE 1850
LLAQSLSSSL LEEKEENKRF EDQLQQWLSQ DSQAFTESTR LPLYLPQTLV 1900
SFPDSIKTQT MVKTSTSPQN SGTGKQLRFS EASGSSLTEK LKLLERLIQS 1950
SRAEEAASEL HLSALLEMVD M 1971
Length:1,971
Mass (Da):217,363
Last modified:July 27, 2011 - v2
Checksum:iFA9CDB20D1441B02
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti10 – 112QQ → SS in CAB44241. 1 Publication
Sequence conflicti1017 – 10171V → L in CAB44241. 1 Publication
Sequence conflicti1190 – 11901E → K in CAB44241. 1 Publication
Sequence conflicti1552 – 15521R → G in CAB44241. 1 Publication
Sequence conflicti1702 – 17021V → A in CAB44241. 1 Publication
Sequence conflicti1782 – 17821L → S in CAB44241. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ006590 mRNA. Translation: CAB44241.1.
CH466553 Genomic DNA. Translation: EDL31849.1.
BC052452 mRNA. Translation: AAH52452.1.
CCDSiCCDS23947.1.
RefSeqiNP_062307.2. NM_019434.2.
UniGeneiMm.30098.

Genome annotation databases

EnsembliENSMUST00000170795; ENSMUSP00000125960; ENSMUSG00000001150.
GeneIDi54387.
KEGGimmu:54387.
UCSCiuc007fup.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ006590 mRNA. Translation: CAB44241.1 .
CH466553 Genomic DNA. Translation: EDL31849.1 .
BC052452 mRNA. Translation: AAH52452.1 .
CCDSi CCDS23947.1.
RefSeqi NP_062307.2. NM_019434.2.
UniGenei Mm.30098.

3D structure databases

ProteinModelPortali Q9WUU9.
SMRi Q9WUU9. Positions 1159-1227.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9WUU9. 1 interaction.
MINTi MINT-4101590.

PTM databases

PhosphoSitei Q9WUU9.

Proteomic databases

PaxDbi Q9WUU9.
PRIDEi Q9WUU9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000170795 ; ENSMUSP00000125960 ; ENSMUSG00000001150 .
GeneIDi 54387.
KEGGi mmu:54387.
UCSCi uc007fup.1. mouse.

Organism-specific databases

CTDi 8888.
MGIi MGI:1930089. Mcm3ap.

Phylogenomic databases

eggNOGi COG5079.
GeneTreei ENSGT00530000063781.
HOVERGENi HBG052431.
InParanoidi Q7TS87.
OMAi AHQMKVQ.
OrthoDBi EOG78D7JB.
TreeFami TF105948.

Miscellaneous databases

NextBioi 311220.
PROi Q9WUU9.
SOURCEi Search...

Gene expression databases

Bgeei Q9WUU9.
Genevestigatori Q9WUU9.

Family and domain databases

InterProi IPR005062. SAC3/GANP/Nin1/mts3/eIF-3-p25.
[Graphical view ]
Pfami PF03399. SAC3_GANP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel nuclear phosphoprotein, GANP, is up-regulated in centrocytes of the germinal center and associated with MCM3, a protein essential for DNA replication."
    Kuwahara K., Yoshida M., Kondo E., Sakata A., Watanabe Y., Abe E., Kouno Y., Tomiyasu S., Fujimura S., Tokuhisa T., Kimura H., Ezaki T., Sakaguchi N.
    Blood 95:2321-2328(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH MCM3, TISSUE SPECIFICITY.
    Strain: BALB/c X NZB.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  4. "Germinal center-associated nuclear protein (GANP) has a phosphorylation-dependent DNA-primase activity that is up-regulated in germinal center regions."
    Kuwahara K., Tomiyasu S., Fujimura S., Nomura K., Xing Y., Nishiyama N., Ogawa M., Imajoh-Ohmi S., Izuta S., Sakaguchi N.
    Proc. Natl. Acad. Sci. U.S.A. 98:10279-10283(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION AT SER-502, MUTAGENESIS OF SER-502.
  5. "GANP suppresses DNA recombination, measured by direct-repeat beta-galactosidase gene construct, but does not suppress the type of recombination applying to immunoglobulin genes in mammalian cells."
    Yoshida M., Kuwahara K., Shimasaki T., Nakagata N., Matsuoka M., Sakaguchi N.
    Genes Cells 12:1205-1213(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  6. "Molecular mechanism of immunoglobulin V-region diversification regulated by transcription and RNA metabolism in antigen-driven B cells."
    Sakaguchi N., Maeda K., Kuwahara K.
    Scand. J. Immunol. 73:520-526(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH AICDA, SUBCELLULAR LOCATION.
  7. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-483 AND LYS-484, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiGANP_MOUSE
AccessioniPrimary (citable) accession number: Q9WUU9
Secondary accession number(s): Q7TS87
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi