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Q9WUU9

- GANP_MOUSE

UniProt

Q9WUU9 - GANP_MOUSE

Protein

Germinal-center associated nuclear protein

Gene

Mcm3ap

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Involved in the nuclear export of poly(A)-containing mRNAs by acting as a scaffold for the TREX-2 complex. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery By similarity. Essential for the generation of high-affinity B-cells against T-cell-dependent antigens by affecting somatic hypermutation at the IgV-regions. May have stimulation-dependent DNA primase activity that would generate extra RNA primers in very rapidely proliferating cells and would support clonal expansion of differentiating B-cells.By similarity2 Publications

    GO - Biological processi

    1. immune system process Source: UniProtKB-KW
    2. mRNA transport Source: UniProtKB-KW
    3. protein transport Source: UniProtKB-KW

    Keywords - Biological processi

    Immunity, mRNA transport, Protein transport, Translocation, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Germinal-center associated nuclear protein
    Short name:
    GANP
    Alternative name(s):
    GC-associated DNA primase
    Gene namesi
    Name:Mcm3ap
    Synonyms:Ganp, Map80
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:1930089. Mcm3ap.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication. Nucleusnuclear pore complex By similarity
    Note: In B-cells nuclear import depends on interaction with AICDA/AID, and GANP is selectively targeted to the IgV-region gene.

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. cytosol Source: Ensembl
    3. nuclear pore Source: UniProtKB-SubCell
    4. nucleus Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Nuclear pore complex, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Causes embryonic death at E12.5 with multiple organ impairments.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi502 – 5021S → A: Loss of DNA primase activity. 1 Publication
    Mutagenesisi502 – 5021S → D: Higher DNA primase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 19711971Germinal-center associated nuclear proteinPRO_0000096285Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei483 – 4831N6-acetyllysine1 Publication
    Modified residuei484 – 4841N6-acetyllysine1 Publication
    Modified residuei502 – 5021Phosphoserine1 Publication
    Modified residuei531 – 5311PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylation at Ser-502 by CDKs is required for DNA primase activity.1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ9WUU9.
    PRIDEiQ9WUU9.

    PTM databases

    PhosphoSiteiQ9WUU9.

    Expressioni

    Tissue specificityi

    Ubiquitous, up-regulated in B-cells germinal centers during T-cell-dependent immune response.2 Publications

    Gene expression databases

    BgeeiQ9WUU9.
    GenevestigatoriQ9WUU9.

    Interactioni

    Subunit structurei

    Component of the nuclear pore complex (NPC)-associated TREX-2 complex (transcription and export complex 2), composed of at least GANP, two copies of ENY2, PCID2, DSS1, and either centrin CETN2 or CETN3. Interacts with NXF1 (By via FG-repeats) By similarity. Interacts with MCM3. Interacts with AICDA/AID.By similarity2 Publications

    Protein-protein interaction databases

    IntActiQ9WUU9. 1 interaction.
    MINTiMINT-4101590.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9WUU9.
    SMRiQ9WUU9. Positions 1159-1227.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni33 – 335303FG-repeatsAdd
    BLAST
    Regioni414 – 550137DNA primaseAdd
    BLAST
    Regioni629 – 983355SAC3 homologyAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili1124 – 116239Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the SAC3 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG5079.
    GeneTreeiENSGT00530000063781.
    HOVERGENiHBG052431.
    InParanoidiQ7TS87.
    OMAiAHQMKVQ.
    OrthoDBiEOG78D7JB.
    TreeFamiTF105948.

    Family and domain databases

    InterProiIPR005062. SAC3/GANP/Nin1/mts3/eIF-3-p25.
    [Graphical view]
    PfamiPF03399. SAC3_GANP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9WUU9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHPVNPFGGQ QPSAFAVSSS TTGTYQTKSP FRFGQPSLFG QNSTPSKSLA     50
    FSQVPSFATP SGGSHSSSLP AFGLTQTSSV GLFSSLESTP SFAATSSSSV 100
    PGNTAFSFKS TSSVGVFPSG ATFGPETGEV AGSGFRKTEF KFKPLENAVF 150
    KPIPGPESEP EKTQSQISSG FFTFSHPVGS GSGGLTPFSF PQVTNSSVTS 200
    SSFIFSKPVT SNTPAFASPL SNQNVEEEKR VSTSAFGSSN SSFSTFPTAS 250
    PGSLGEPFPA NKPSLRQGCE EAISQVEPLP TLMKGLKRKE DQDRSPRRHC 300
    HEAAEDPDPL SRGDHPPDKR PVRLNRPRGG TLFGRTIQEV FKSNKEAGRL 350
    GSKESKESGF AEPGESDHAA VPGGSQSTMV PSRLPAVTKE EEESRDEKED 400
    SLRGKSVRQS KRREEWIYSL GGVSSLELTA IQCKNIPDYL NDRAILEKHF 450
    SKIAKVQRVF TRRSKKLAVI HFFDHASAAL ARKKGKGLHK DVVIFWHKKK 500
    ISPSKKLFPL KEKLGESEAS QGIEDSPFQH SPLSKPIVRP AAGSLLSKSS 550
    PVKKPSLLKM HQFEADPFDS GSEGSEGLGS CVSSLSTLIG TVADTSEEKY 600
    RLLDQRDRIM RQARVKRTDL DKARAFVGTC PDMCPEKERY LRETRSQLSV 650
    FEVVPGTDQV DHAAAVKEYS RSSADQEEPL PHELRPSAVL SRTMDYLVTQ 700
    IMDQKEGSLR DWYDFVWNRT RGIRKDITQQ HLCDPLTVSL IEKCTRFHIH 750
    CAHFMCEEPM SSFDAKINNE NMTKCLQSLK EMYQDLRNKG VFCASEAEFQ 800
    GYNVLLNLNK GDILREVQQF HPDVRNSPEV NFAVQAFAAL NSNNFVRFFK 850
    LVQSASYLNA CLLHCYFNQI RKDALRALNV AYTVSTQRST VFPLDGVVRM 900
    LLFRDSEEAT NFLNYHGLTV ADGCVELNRS AFLEPEGLCK ARKSVFIGRK 950
    LTVSVGEVVN GGPLPPVPRH TPVCSFNSQN KYVGESLATE LPISTQRAGG 1000
    DPAGGGRGED CEAEVDVPTL AVLPQPPPAS SATPALHVQP LAPAAAPSLL 1050
    QASTQPEVLL PKPAPVYSDS DLVQVVDELI QEALQVDCEE VSSAGAAYVA 1100
    AALGVSNAAV EDLITAATTG ILRHVAAEEV SMERQRLEEE KQRAEEERLK 1150
    QERELMLTQL SEGLAAELTE LTVTECVWET CSQELQSAVE IDQKVRVARC 1200
    CEAVCAHLVD LFLAEEIFQT AKETLQELQC FCKYLQRWRE AVAARKKFRR 1250
    QMRAFPAAPC CVDVNDRLQA LVPSAECPIT EENLAKGLLD LGHAGKVGVS 1300
    CTRLRRLRNK TAHQIKVQHF HQQLLRNAAW APLDLPSIVS EHLPMKQKRR 1350
    FWKLVLVLPD VEEQTPESPG RILENWLKVK FTGDDSMVGD IGDNAGDIQT 1400
    LSVFNTLSSK GDQTVSVNVC IKVAHGTLSD SALDAVETQK DLLGTSGLML 1450
    LLPPKVKSEE VAEEELSWLS ALLQLKQLLQ AKPFQPALPL VVLVPSSRGD 1500
    SAGRAVEDGL MLQDLVSAKL ISDYIVVEIP DSVNDLQGTV KVSGAVQWLI 1550
    SRCPQALDLC CQTLVQYVED GISREFSRRF FHDRRERRLA SLPSQEPSTI 1600
    IELFNSVLQF LASVVSSEQL CDISWPVMEF AEVGGSQLLP HLHWNSPEHL 1650
    AWLKQAVLGF QLPQMDLPPP GAPWLPVCSM VIQYTSQIPS SSQTQPVLQS 1700
    QVENLLCRTY QKWKNKSLSP GQELGPSVAE IPWDDIITLC INHKLRDWTP 1750
    PRLPVTLEAL SEDGQICVYF FKNLLRKYHV PLSWEQARMQ TQRELQLSHG 1800
    RSGMRSIHPP TSTFPTPLLH VHQKGKKKEE SGREGSLSTE DLLRGASAEE 1850
    LLAQSLSSSL LEEKEENKRF EDQLQQWLSQ DSQAFTESTR LPLYLPQTLV 1900
    SFPDSIKTQT MVKTSTSPQN SGTGKQLRFS EASGSSLTEK LKLLERLIQS 1950
    SRAEEAASEL HLSALLEMVD M 1971
    Length:1,971
    Mass (Da):217,363
    Last modified:July 27, 2011 - v2
    Checksum:iFA9CDB20D1441B02
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti10 – 112QQ → SS in CAB44241. (PubMed:10733502)Curated
    Sequence conflicti1017 – 10171V → L in CAB44241. (PubMed:10733502)Curated
    Sequence conflicti1190 – 11901E → K in CAB44241. (PubMed:10733502)Curated
    Sequence conflicti1552 – 15521R → G in CAB44241. (PubMed:10733502)Curated
    Sequence conflicti1702 – 17021V → A in CAB44241. (PubMed:10733502)Curated
    Sequence conflicti1782 – 17821L → S in CAB44241. (PubMed:10733502)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ006590 mRNA. Translation: CAB44241.1.
    CH466553 Genomic DNA. Translation: EDL31849.1.
    BC052452 mRNA. Translation: AAH52452.1.
    CCDSiCCDS23947.1.
    RefSeqiNP_062307.2. NM_019434.2.
    UniGeneiMm.30098.

    Genome annotation databases

    EnsembliENSMUST00000170795; ENSMUSP00000125960; ENSMUSG00000001150.
    GeneIDi54387.
    KEGGimmu:54387.
    UCSCiuc007fup.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ006590 mRNA. Translation: CAB44241.1 .
    CH466553 Genomic DNA. Translation: EDL31849.1 .
    BC052452 mRNA. Translation: AAH52452.1 .
    CCDSi CCDS23947.1.
    RefSeqi NP_062307.2. NM_019434.2.
    UniGenei Mm.30098.

    3D structure databases

    ProteinModelPortali Q9WUU9.
    SMRi Q9WUU9. Positions 1159-1227.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9WUU9. 1 interaction.
    MINTi MINT-4101590.

    PTM databases

    PhosphoSitei Q9WUU9.

    Proteomic databases

    PaxDbi Q9WUU9.
    PRIDEi Q9WUU9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000170795 ; ENSMUSP00000125960 ; ENSMUSG00000001150 .
    GeneIDi 54387.
    KEGGi mmu:54387.
    UCSCi uc007fup.1. mouse.

    Organism-specific databases

    CTDi 8888.
    MGIi MGI:1930089. Mcm3ap.

    Phylogenomic databases

    eggNOGi COG5079.
    GeneTreei ENSGT00530000063781.
    HOVERGENi HBG052431.
    InParanoidi Q7TS87.
    OMAi AHQMKVQ.
    OrthoDBi EOG78D7JB.
    TreeFami TF105948.

    Miscellaneous databases

    NextBioi 311220.
    PROi Q9WUU9.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9WUU9.
    Genevestigatori Q9WUU9.

    Family and domain databases

    InterProi IPR005062. SAC3/GANP/Nin1/mts3/eIF-3-p25.
    [Graphical view ]
    Pfami PF03399. SAC3_GANP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel nuclear phosphoprotein, GANP, is up-regulated in centrocytes of the germinal center and associated with MCM3, a protein essential for DNA replication."
      Kuwahara K., Yoshida M., Kondo E., Sakata A., Watanabe Y., Abe E., Kouno Y., Tomiyasu S., Fujimura S., Tokuhisa T., Kimura H., Ezaki T., Sakaguchi N.
      Blood 95:2321-2328(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH MCM3, TISSUE SPECIFICITY.
      Strain: BALB/c X NZB.
    2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    4. "Germinal center-associated nuclear protein (GANP) has a phosphorylation-dependent DNA-primase activity that is up-regulated in germinal center regions."
      Kuwahara K., Tomiyasu S., Fujimura S., Nomura K., Xing Y., Nishiyama N., Ogawa M., Imajoh-Ohmi S., Izuta S., Sakaguchi N.
      Proc. Natl. Acad. Sci. U.S.A. 98:10279-10283(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION AT SER-502, MUTAGENESIS OF SER-502.
    5. "GANP suppresses DNA recombination, measured by direct-repeat beta-galactosidase gene construct, but does not suppress the type of recombination applying to immunoglobulin genes in mammalian cells."
      Yoshida M., Kuwahara K., Shimasaki T., Nakagata N., Matsuoka M., Sakaguchi N.
      Genes Cells 12:1205-1213(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    6. "Molecular mechanism of immunoglobulin V-region diversification regulated by transcription and RNA metabolism in antigen-driven B cells."
      Sakaguchi N., Maeda K., Kuwahara K.
      Scand. J. Immunol. 73:520-526(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH AICDA, SUBCELLULAR LOCATION.
    7. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
      Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
      Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-483 AND LYS-484, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiGANP_MOUSE
    AccessioniPrimary (citable) accession number: Q9WUU9
    Secondary accession number(s): Q7TS87
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 91 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3