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Protein

Germinal-center associated nuclear protein

Gene

Mcm3ap

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the nuclear export of poly(A)-containing mRNAs by acting as a scaffold for the TREX-2 complex. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery (By similarity). Essential for the generation of high-affinity B-cells against T-cell-dependent antigens by affecting somatic hypermutation at the IgV-regions. May have stimulation-dependent DNA primase activity that would generate extra RNA primers in very rapidely proliferating cells and would support clonal expansion of differentiating B-cells.By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processImmunity, mRNA transport, Protein transport, Translocation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Germinal-center associated nuclear protein
Short name:
GANP
Alternative name(s):
GC-associated DNA primase
Gene namesi
Name:Mcm3ap
Synonyms:Ganp, Map80
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1930089 Mcm3ap

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nuclear pore complex, Nucleus

Pathology & Biotechi

Disruption phenotypei

Causes embryonic death at E12.5 with multiple organ impairments.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi502S → A: Loss of DNA primase activity. 1 Publication1
Mutagenesisi502S → D: Higher DNA primase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000962851 – 1971Germinal-center associated nuclear proteinAdd BLAST1971

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei32Asymmetric dimethylarginineCombined sources1
Modified residuei424PhosphoserineBy similarity1
Modified residuei483N6-acetyllysineCombined sources1
Modified residuei484N6-acetyllysineCombined sources1
Modified residuei502Phosphoserine1 Publication1
Modified residuei531PhosphoserineBy similarity1
Modified residuei550PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation at Ser-502 by CDKs is required for DNA primase activity.1 Publication

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ9WUU9
MaxQBiQ9WUU9
PaxDbiQ9WUU9
PeptideAtlasiQ9WUU9
PRIDEiQ9WUU9

PTM databases

iPTMnetiQ9WUU9
PhosphoSitePlusiQ9WUU9

Expressioni

Tissue specificityi

Ubiquitous, up-regulated in B-cells germinal centers during T-cell-dependent immune response.2 Publications

Gene expression databases

BgeeiENSMUSG00000001150
ExpressionAtlasiQ9WUU9 baseline and differential
GenevisibleiQ9WUU9 MM

Interactioni

Subunit structurei

Component of the nuclear pore complex (NPC)-associated TREX-2 complex (transcription and export complex 2), composed of at least GANP, two copies of ENY2, PCID2, SEM1, and either centrin CETN2 or CETN3. Interacts with NXF1 (By via FG-repeats) (By similarity). Interacts with MCM3. Interacts with AICDA/AID.By similarity2 Publications

Protein-protein interaction databases

IntActiQ9WUU9, 1 interactor
MINTiQ9WUU9
STRINGi10090.ENSMUSP00000125960

Structurei

3D structure databases

ProteinModelPortaliQ9WUU9
SMRiQ9WUU9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini768 – 951PCIPROSITE-ProRule annotationAdd BLAST184

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni33 – 335FG-repeatsAdd BLAST303
Regioni414 – 550DNA primaseAdd BLAST137

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1124 – 1162Sequence analysisAdd BLAST39

Sequence similaritiesi

Belongs to the SAC3 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1860 Eukaryota
COG5079 LUCA
GeneTreeiENSGT00530000063781
HOVERGENiHBG052431
InParanoidiQ9WUU9
OMAiNSSEVKF
OrthoDBiEOG091G01V4
TreeFamiTF105948

Family and domain databases

CDDicd12443 RRM_MCM3A_like, 1 hit
InterProiView protein in InterPro
IPR031910 GANP_CID_dom
IPR031907 MCM3AP_GANP
IPR034265 MCM3AP_RRM
IPR031908 NupH_GANP
IPR000717 PCI_dom
IPR035979 RBD_domain_sf
IPR005062 SAC3/GANP/THP3
PfamiView protein in Pfam
PF16766 CID_GANP, 1 hit
PF16769 MCM3AP_GANP, 1 hit
PF16768 NupH_GANP, 1 hit
PF03399 SAC3_GANP, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50250 PCI, 1 hit

Sequencei

Sequence statusi: Complete.

Q9WUU9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHPVNPFGGQ QPSAFAVSSS TTGTYQTKSP FRFGQPSLFG QNSTPSKSLA
60 70 80 90 100
FSQVPSFATP SGGSHSSSLP AFGLTQTSSV GLFSSLESTP SFAATSSSSV
110 120 130 140 150
PGNTAFSFKS TSSVGVFPSG ATFGPETGEV AGSGFRKTEF KFKPLENAVF
160 170 180 190 200
KPIPGPESEP EKTQSQISSG FFTFSHPVGS GSGGLTPFSF PQVTNSSVTS
210 220 230 240 250
SSFIFSKPVT SNTPAFASPL SNQNVEEEKR VSTSAFGSSN SSFSTFPTAS
260 270 280 290 300
PGSLGEPFPA NKPSLRQGCE EAISQVEPLP TLMKGLKRKE DQDRSPRRHC
310 320 330 340 350
HEAAEDPDPL SRGDHPPDKR PVRLNRPRGG TLFGRTIQEV FKSNKEAGRL
360 370 380 390 400
GSKESKESGF AEPGESDHAA VPGGSQSTMV PSRLPAVTKE EEESRDEKED
410 420 430 440 450
SLRGKSVRQS KRREEWIYSL GGVSSLELTA IQCKNIPDYL NDRAILEKHF
460 470 480 490 500
SKIAKVQRVF TRRSKKLAVI HFFDHASAAL ARKKGKGLHK DVVIFWHKKK
510 520 530 540 550
ISPSKKLFPL KEKLGESEAS QGIEDSPFQH SPLSKPIVRP AAGSLLSKSS
560 570 580 590 600
PVKKPSLLKM HQFEADPFDS GSEGSEGLGS CVSSLSTLIG TVADTSEEKY
610 620 630 640 650
RLLDQRDRIM RQARVKRTDL DKARAFVGTC PDMCPEKERY LRETRSQLSV
660 670 680 690 700
FEVVPGTDQV DHAAAVKEYS RSSADQEEPL PHELRPSAVL SRTMDYLVTQ
710 720 730 740 750
IMDQKEGSLR DWYDFVWNRT RGIRKDITQQ HLCDPLTVSL IEKCTRFHIH
760 770 780 790 800
CAHFMCEEPM SSFDAKINNE NMTKCLQSLK EMYQDLRNKG VFCASEAEFQ
810 820 830 840 850
GYNVLLNLNK GDILREVQQF HPDVRNSPEV NFAVQAFAAL NSNNFVRFFK
860 870 880 890 900
LVQSASYLNA CLLHCYFNQI RKDALRALNV AYTVSTQRST VFPLDGVVRM
910 920 930 940 950
LLFRDSEEAT NFLNYHGLTV ADGCVELNRS AFLEPEGLCK ARKSVFIGRK
960 970 980 990 1000
LTVSVGEVVN GGPLPPVPRH TPVCSFNSQN KYVGESLATE LPISTQRAGG
1010 1020 1030 1040 1050
DPAGGGRGED CEAEVDVPTL AVLPQPPPAS SATPALHVQP LAPAAAPSLL
1060 1070 1080 1090 1100
QASTQPEVLL PKPAPVYSDS DLVQVVDELI QEALQVDCEE VSSAGAAYVA
1110 1120 1130 1140 1150
AALGVSNAAV EDLITAATTG ILRHVAAEEV SMERQRLEEE KQRAEEERLK
1160 1170 1180 1190 1200
QERELMLTQL SEGLAAELTE LTVTECVWET CSQELQSAVE IDQKVRVARC
1210 1220 1230 1240 1250
CEAVCAHLVD LFLAEEIFQT AKETLQELQC FCKYLQRWRE AVAARKKFRR
1260 1270 1280 1290 1300
QMRAFPAAPC CVDVNDRLQA LVPSAECPIT EENLAKGLLD LGHAGKVGVS
1310 1320 1330 1340 1350
CTRLRRLRNK TAHQIKVQHF HQQLLRNAAW APLDLPSIVS EHLPMKQKRR
1360 1370 1380 1390 1400
FWKLVLVLPD VEEQTPESPG RILENWLKVK FTGDDSMVGD IGDNAGDIQT
1410 1420 1430 1440 1450
LSVFNTLSSK GDQTVSVNVC IKVAHGTLSD SALDAVETQK DLLGTSGLML
1460 1470 1480 1490 1500
LLPPKVKSEE VAEEELSWLS ALLQLKQLLQ AKPFQPALPL VVLVPSSRGD
1510 1520 1530 1540 1550
SAGRAVEDGL MLQDLVSAKL ISDYIVVEIP DSVNDLQGTV KVSGAVQWLI
1560 1570 1580 1590 1600
SRCPQALDLC CQTLVQYVED GISREFSRRF FHDRRERRLA SLPSQEPSTI
1610 1620 1630 1640 1650
IELFNSVLQF LASVVSSEQL CDISWPVMEF AEVGGSQLLP HLHWNSPEHL
1660 1670 1680 1690 1700
AWLKQAVLGF QLPQMDLPPP GAPWLPVCSM VIQYTSQIPS SSQTQPVLQS
1710 1720 1730 1740 1750
QVENLLCRTY QKWKNKSLSP GQELGPSVAE IPWDDIITLC INHKLRDWTP
1760 1770 1780 1790 1800
PRLPVTLEAL SEDGQICVYF FKNLLRKYHV PLSWEQARMQ TQRELQLSHG
1810 1820 1830 1840 1850
RSGMRSIHPP TSTFPTPLLH VHQKGKKKEE SGREGSLSTE DLLRGASAEE
1860 1870 1880 1890 1900
LLAQSLSSSL LEEKEENKRF EDQLQQWLSQ DSQAFTESTR LPLYLPQTLV
1910 1920 1930 1940 1950
SFPDSIKTQT MVKTSTSPQN SGTGKQLRFS EASGSSLTEK LKLLERLIQS
1960 1970
SRAEEAASEL HLSALLEMVD M
Length:1,971
Mass (Da):217,363
Last modified:July 27, 2011 - v2
Checksum:iFA9CDB20D1441B02
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti10 – 11QQ → SS in CAB44241 (PubMed:10733502).Curated2
Sequence conflicti1017V → L in CAB44241 (PubMed:10733502).Curated1
Sequence conflicti1190E → K in CAB44241 (PubMed:10733502).Curated1
Sequence conflicti1552R → G in CAB44241 (PubMed:10733502).Curated1
Sequence conflicti1702V → A in CAB44241 (PubMed:10733502).Curated1
Sequence conflicti1782L → S in CAB44241 (PubMed:10733502).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ006590 mRNA Translation: CAB44241.1
CH466553 Genomic DNA Translation: EDL31849.1
BC052452 mRNA Translation: AAH52452.1
CCDSiCCDS23947.1
RefSeqiNP_062307.2, NM_019434.2
UniGeneiMm.30098

Genome annotation databases

EnsembliENSMUST00000170795; ENSMUSP00000125960; ENSMUSG00000001150
GeneIDi54387
KEGGimmu:54387
UCSCiuc007fup.1 mouse

Similar proteinsi

Entry informationi

Entry nameiGANP_MOUSE
AccessioniPrimary (citable) accession number: Q9WUU9
Secondary accession number(s): Q7TS87
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 118 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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