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Q9WUU9

- GANP_MOUSE

UniProt

Q9WUU9 - GANP_MOUSE

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Protein

Germinal-center associated nuclear protein

Gene

Mcm3ap

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the nuclear export of poly(A)-containing mRNAs by acting as a scaffold for the TREX-2 complex. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery (By similarity). Essential for the generation of high-affinity B-cells against T-cell-dependent antigens by affecting somatic hypermutation at the IgV-regions. May have stimulation-dependent DNA primase activity that would generate extra RNA primers in very rapidely proliferating cells and would support clonal expansion of differentiating B-cells.By similarity2 Publications

GO - Biological processi

  1. immune system process Source: UniProtKB-KW
  2. mRNA transport Source: UniProtKB-KW
  3. protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Immunity, mRNA transport, Protein transport, Translocation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Germinal-center associated nuclear protein
Short name:
GANP
Alternative name(s):
GC-associated DNA primase
Gene namesi
Name:Mcm3ap
Synonyms:Ganp, Map80
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:1930089. Mcm3ap.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication. Nucleusnuclear pore complex By similarity
Note: In B-cells nuclear import depends on interaction with AICDA/AID, and GANP is selectively targeted to the IgV-region gene.

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. cytosol Source: Ensembl
  3. nuclear pore Source: UniProtKB-KW
  4. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nuclear pore complex, Nucleus

Pathology & Biotechi

Disruption phenotypei

Causes embryonic death at E12.5 with multiple organ impairments.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi502 – 5021S → A: Loss of DNA primase activity. 1 Publication
Mutagenesisi502 – 5021S → D: Higher DNA primase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 19711971Germinal-center associated nuclear proteinPRO_0000096285Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei483 – 4831N6-acetyllysine1 Publication
Modified residuei484 – 4841N6-acetyllysine1 Publication
Modified residuei502 – 5021Phosphoserine1 Publication
Modified residuei531 – 5311PhosphoserineBy similarity

Post-translational modificationi

Phosphorylation at Ser-502 by CDKs is required for DNA primase activity.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9WUU9.
PaxDbiQ9WUU9.
PRIDEiQ9WUU9.

PTM databases

PhosphoSiteiQ9WUU9.

Expressioni

Tissue specificityi

Ubiquitous, up-regulated in B-cells germinal centers during T-cell-dependent immune response.2 Publications

Gene expression databases

BgeeiQ9WUU9.
ExpressionAtlasiQ9WUU9. baseline.
GenevestigatoriQ9WUU9.

Interactioni

Subunit structurei

Component of the nuclear pore complex (NPC)-associated TREX-2 complex (transcription and export complex 2), composed of at least GANP, two copies of ENY2, PCID2, DSS1, and either centrin CETN2 or CETN3. Interacts with NXF1 (By via FG-repeats) (By similarity). Interacts with MCM3. Interacts with AICDA/AID.By similarity2 Publications

Protein-protein interaction databases

IntActiQ9WUU9. 1 interaction.
MINTiMINT-4101590.

Structurei

3D structure databases

ProteinModelPortaliQ9WUU9.
SMRiQ9WUU9. Positions 1159-1227.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni33 – 335303FG-repeatsAdd
BLAST
Regioni414 – 550137DNA primaseAdd
BLAST
Regioni629 – 983355SAC3 homologyAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1124 – 116239Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the SAC3 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5079.
GeneTreeiENSGT00530000063781.
HOVERGENiHBG052431.
InParanoidiQ9WUU9.
OMAiAHQMKVQ.
OrthoDBiEOG78D7JB.
TreeFamiTF105948.

Family and domain databases

InterProiIPR005062. SAC3/GANP/Nin1/mts3/eIF-3-p25.
[Graphical view]
PfamiPF03399. SAC3_GANP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WUU9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHPVNPFGGQ QPSAFAVSSS TTGTYQTKSP FRFGQPSLFG QNSTPSKSLA
60 70 80 90 100
FSQVPSFATP SGGSHSSSLP AFGLTQTSSV GLFSSLESTP SFAATSSSSV
110 120 130 140 150
PGNTAFSFKS TSSVGVFPSG ATFGPETGEV AGSGFRKTEF KFKPLENAVF
160 170 180 190 200
KPIPGPESEP EKTQSQISSG FFTFSHPVGS GSGGLTPFSF PQVTNSSVTS
210 220 230 240 250
SSFIFSKPVT SNTPAFASPL SNQNVEEEKR VSTSAFGSSN SSFSTFPTAS
260 270 280 290 300
PGSLGEPFPA NKPSLRQGCE EAISQVEPLP TLMKGLKRKE DQDRSPRRHC
310 320 330 340 350
HEAAEDPDPL SRGDHPPDKR PVRLNRPRGG TLFGRTIQEV FKSNKEAGRL
360 370 380 390 400
GSKESKESGF AEPGESDHAA VPGGSQSTMV PSRLPAVTKE EEESRDEKED
410 420 430 440 450
SLRGKSVRQS KRREEWIYSL GGVSSLELTA IQCKNIPDYL NDRAILEKHF
460 470 480 490 500
SKIAKVQRVF TRRSKKLAVI HFFDHASAAL ARKKGKGLHK DVVIFWHKKK
510 520 530 540 550
ISPSKKLFPL KEKLGESEAS QGIEDSPFQH SPLSKPIVRP AAGSLLSKSS
560 570 580 590 600
PVKKPSLLKM HQFEADPFDS GSEGSEGLGS CVSSLSTLIG TVADTSEEKY
610 620 630 640 650
RLLDQRDRIM RQARVKRTDL DKARAFVGTC PDMCPEKERY LRETRSQLSV
660 670 680 690 700
FEVVPGTDQV DHAAAVKEYS RSSADQEEPL PHELRPSAVL SRTMDYLVTQ
710 720 730 740 750
IMDQKEGSLR DWYDFVWNRT RGIRKDITQQ HLCDPLTVSL IEKCTRFHIH
760 770 780 790 800
CAHFMCEEPM SSFDAKINNE NMTKCLQSLK EMYQDLRNKG VFCASEAEFQ
810 820 830 840 850
GYNVLLNLNK GDILREVQQF HPDVRNSPEV NFAVQAFAAL NSNNFVRFFK
860 870 880 890 900
LVQSASYLNA CLLHCYFNQI RKDALRALNV AYTVSTQRST VFPLDGVVRM
910 920 930 940 950
LLFRDSEEAT NFLNYHGLTV ADGCVELNRS AFLEPEGLCK ARKSVFIGRK
960 970 980 990 1000
LTVSVGEVVN GGPLPPVPRH TPVCSFNSQN KYVGESLATE LPISTQRAGG
1010 1020 1030 1040 1050
DPAGGGRGED CEAEVDVPTL AVLPQPPPAS SATPALHVQP LAPAAAPSLL
1060 1070 1080 1090 1100
QASTQPEVLL PKPAPVYSDS DLVQVVDELI QEALQVDCEE VSSAGAAYVA
1110 1120 1130 1140 1150
AALGVSNAAV EDLITAATTG ILRHVAAEEV SMERQRLEEE KQRAEEERLK
1160 1170 1180 1190 1200
QERELMLTQL SEGLAAELTE LTVTECVWET CSQELQSAVE IDQKVRVARC
1210 1220 1230 1240 1250
CEAVCAHLVD LFLAEEIFQT AKETLQELQC FCKYLQRWRE AVAARKKFRR
1260 1270 1280 1290 1300
QMRAFPAAPC CVDVNDRLQA LVPSAECPIT EENLAKGLLD LGHAGKVGVS
1310 1320 1330 1340 1350
CTRLRRLRNK TAHQIKVQHF HQQLLRNAAW APLDLPSIVS EHLPMKQKRR
1360 1370 1380 1390 1400
FWKLVLVLPD VEEQTPESPG RILENWLKVK FTGDDSMVGD IGDNAGDIQT
1410 1420 1430 1440 1450
LSVFNTLSSK GDQTVSVNVC IKVAHGTLSD SALDAVETQK DLLGTSGLML
1460 1470 1480 1490 1500
LLPPKVKSEE VAEEELSWLS ALLQLKQLLQ AKPFQPALPL VVLVPSSRGD
1510 1520 1530 1540 1550
SAGRAVEDGL MLQDLVSAKL ISDYIVVEIP DSVNDLQGTV KVSGAVQWLI
1560 1570 1580 1590 1600
SRCPQALDLC CQTLVQYVED GISREFSRRF FHDRRERRLA SLPSQEPSTI
1610 1620 1630 1640 1650
IELFNSVLQF LASVVSSEQL CDISWPVMEF AEVGGSQLLP HLHWNSPEHL
1660 1670 1680 1690 1700
AWLKQAVLGF QLPQMDLPPP GAPWLPVCSM VIQYTSQIPS SSQTQPVLQS
1710 1720 1730 1740 1750
QVENLLCRTY QKWKNKSLSP GQELGPSVAE IPWDDIITLC INHKLRDWTP
1760 1770 1780 1790 1800
PRLPVTLEAL SEDGQICVYF FKNLLRKYHV PLSWEQARMQ TQRELQLSHG
1810 1820 1830 1840 1850
RSGMRSIHPP TSTFPTPLLH VHQKGKKKEE SGREGSLSTE DLLRGASAEE
1860 1870 1880 1890 1900
LLAQSLSSSL LEEKEENKRF EDQLQQWLSQ DSQAFTESTR LPLYLPQTLV
1910 1920 1930 1940 1950
SFPDSIKTQT MVKTSTSPQN SGTGKQLRFS EASGSSLTEK LKLLERLIQS
1960 1970
SRAEEAASEL HLSALLEMVD M
Length:1,971
Mass (Da):217,363
Last modified:July 27, 2011 - v2
Checksum:iFA9CDB20D1441B02
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti10 – 112QQ → SS in CAB44241. (PubMed:10733502)Curated
Sequence conflicti1017 – 10171V → L in CAB44241. (PubMed:10733502)Curated
Sequence conflicti1190 – 11901E → K in CAB44241. (PubMed:10733502)Curated
Sequence conflicti1552 – 15521R → G in CAB44241. (PubMed:10733502)Curated
Sequence conflicti1702 – 17021V → A in CAB44241. (PubMed:10733502)Curated
Sequence conflicti1782 – 17821L → S in CAB44241. (PubMed:10733502)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ006590 mRNA. Translation: CAB44241.1.
CH466553 Genomic DNA. Translation: EDL31849.1.
BC052452 mRNA. Translation: AAH52452.1.
CCDSiCCDS23947.1.
RefSeqiNP_062307.2. NM_019434.2.
UniGeneiMm.30098.

Genome annotation databases

EnsembliENSMUST00000170795; ENSMUSP00000125960; ENSMUSG00000001150.
GeneIDi54387.
KEGGimmu:54387.
UCSCiuc007fup.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ006590 mRNA. Translation: CAB44241.1 .
CH466553 Genomic DNA. Translation: EDL31849.1 .
BC052452 mRNA. Translation: AAH52452.1 .
CCDSi CCDS23947.1.
RefSeqi NP_062307.2. NM_019434.2.
UniGenei Mm.30098.

3D structure databases

ProteinModelPortali Q9WUU9.
SMRi Q9WUU9. Positions 1159-1227.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9WUU9. 1 interaction.
MINTi MINT-4101590.

PTM databases

PhosphoSitei Q9WUU9.

Proteomic databases

MaxQBi Q9WUU9.
PaxDbi Q9WUU9.
PRIDEi Q9WUU9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000170795 ; ENSMUSP00000125960 ; ENSMUSG00000001150 .
GeneIDi 54387.
KEGGi mmu:54387.
UCSCi uc007fup.1. mouse.

Organism-specific databases

CTDi 8888.
MGIi MGI:1930089. Mcm3ap.

Phylogenomic databases

eggNOGi COG5079.
GeneTreei ENSGT00530000063781.
HOVERGENi HBG052431.
InParanoidi Q9WUU9.
OMAi AHQMKVQ.
OrthoDBi EOG78D7JB.
TreeFami TF105948.

Miscellaneous databases

NextBioi 311220.
PROi Q9WUU9.
SOURCEi Search...

Gene expression databases

Bgeei Q9WUU9.
ExpressionAtlasi Q9WUU9. baseline.
Genevestigatori Q9WUU9.

Family and domain databases

InterProi IPR005062. SAC3/GANP/Nin1/mts3/eIF-3-p25.
[Graphical view ]
Pfami PF03399. SAC3_GANP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel nuclear phosphoprotein, GANP, is up-regulated in centrocytes of the germinal center and associated with MCM3, a protein essential for DNA replication."
    Kuwahara K., Yoshida M., Kondo E., Sakata A., Watanabe Y., Abe E., Kouno Y., Tomiyasu S., Fujimura S., Tokuhisa T., Kimura H., Ezaki T., Sakaguchi N.
    Blood 95:2321-2328(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH MCM3, TISSUE SPECIFICITY.
    Strain: BALB/c X NZB.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  4. "Germinal center-associated nuclear protein (GANP) has a phosphorylation-dependent DNA-primase activity that is up-regulated in germinal center regions."
    Kuwahara K., Tomiyasu S., Fujimura S., Nomura K., Xing Y., Nishiyama N., Ogawa M., Imajoh-Ohmi S., Izuta S., Sakaguchi N.
    Proc. Natl. Acad. Sci. U.S.A. 98:10279-10283(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION AT SER-502, MUTAGENESIS OF SER-502.
  5. "GANP suppresses DNA recombination, measured by direct-repeat beta-galactosidase gene construct, but does not suppress the type of recombination applying to immunoglobulin genes in mammalian cells."
    Yoshida M., Kuwahara K., Shimasaki T., Nakagata N., Matsuoka M., Sakaguchi N.
    Genes Cells 12:1205-1213(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  6. "Molecular mechanism of immunoglobulin V-region diversification regulated by transcription and RNA metabolism in antigen-driven B cells."
    Sakaguchi N., Maeda K., Kuwahara K.
    Scand. J. Immunol. 73:520-526(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH AICDA, SUBCELLULAR LOCATION.
  7. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-483 AND LYS-484, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiGANP_MOUSE
AccessioniPrimary (citable) accession number: Q9WUU9
Secondary accession number(s): Q7TS87
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3