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Q9WUU9 (GANP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Germinal-center associated nuclear protein

Short name=GANP
Alternative name(s):
GC-associated DNA primase
Gene names
Name:Mcm3ap
Synonyms:Ganp, Map80
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1971 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the nuclear export of poly(A)-containing mRNAs by acting as a scaffold for the TREX-2 complex. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery By similarity. Essential for the generation of high-affinity B-cells against T-cell-dependent antigens by affecting somatic hypermutation at the IgV-regions. May have stimulation-dependent DNA primase activity that would generate extra RNA primers in very rapidely proliferating cells and would support clonal expansion of differentiating B-cells. Ref.4 Ref.6

Subunit structure

Component of the nuclear pore complex (NPC)-associated TREX-2 complex (transcription and export complex 2), composed of at least GANP, two copies of ENY2, PCID2, DSS1, and either centrin CETN2 or CETN3. Interacts with NXF1 (By via FG-repeats) By similarity. Interacts with MCM3. Interacts with AICDA/AID. Ref.1 Ref.6

Subcellular location

Cytoplasm. Nucleus. Nucleusnuclear pore complex By similarity. Note: In B-cells nuclear import depends on interaction with AICDA/AID, and GANP is selectively targeted to the IgV-region gene. Ref.6

Tissue specificity

Ubiquitous, up-regulated in B-cells germinal centers during T-cell-dependent immune response. Ref.1 Ref.6

Post-translational modification

Phosphorylation at Ser-502 by CDKs is required for DNA primase activity.

Disruption phenotype

Causes embryonic death at E12.5 with multiple organ impairments. Ref.5

Sequence similarities

Belongs to the SAC3 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 19711971Germinal-center associated nuclear protein
PRO_0000096285

Regions

Region33 – 335303FG-repeats
Region414 – 550137DNA primase
Region629 – 983355SAC3 homology
Coiled coil1124 – 116239 Potential

Amino acid modifications

Modified residue4831N6-acetyllysine Ref.7
Modified residue4841N6-acetyllysine Ref.7
Modified residue5021Phosphoserine Ref.4
Modified residue5311Phosphoserine By similarity

Experimental info

Mutagenesis5021S → A: Loss of DNA primase activity. Ref.4
Mutagenesis5021S → D: Higher DNA primase activity. Ref.4
Sequence conflict10 – 112QQ → SS in CAB44241. Ref.1
Sequence conflict10171V → L in CAB44241. Ref.1
Sequence conflict11901E → K in CAB44241. Ref.1
Sequence conflict15521R → G in CAB44241. Ref.1
Sequence conflict17021V → A in CAB44241. Ref.1
Sequence conflict17821L → S in CAB44241. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9WUU9 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: FA9CDB20D1441B02

FASTA1,971217,363
        10         20         30         40         50         60 
MHPVNPFGGQ QPSAFAVSSS TTGTYQTKSP FRFGQPSLFG QNSTPSKSLA FSQVPSFATP 

        70         80         90        100        110        120 
SGGSHSSSLP AFGLTQTSSV GLFSSLESTP SFAATSSSSV PGNTAFSFKS TSSVGVFPSG 

       130        140        150        160        170        180 
ATFGPETGEV AGSGFRKTEF KFKPLENAVF KPIPGPESEP EKTQSQISSG FFTFSHPVGS 

       190        200        210        220        230        240 
GSGGLTPFSF PQVTNSSVTS SSFIFSKPVT SNTPAFASPL SNQNVEEEKR VSTSAFGSSN 

       250        260        270        280        290        300 
SSFSTFPTAS PGSLGEPFPA NKPSLRQGCE EAISQVEPLP TLMKGLKRKE DQDRSPRRHC 

       310        320        330        340        350        360 
HEAAEDPDPL SRGDHPPDKR PVRLNRPRGG TLFGRTIQEV FKSNKEAGRL GSKESKESGF 

       370        380        390        400        410        420 
AEPGESDHAA VPGGSQSTMV PSRLPAVTKE EEESRDEKED SLRGKSVRQS KRREEWIYSL 

       430        440        450        460        470        480 
GGVSSLELTA IQCKNIPDYL NDRAILEKHF SKIAKVQRVF TRRSKKLAVI HFFDHASAAL 

       490        500        510        520        530        540 
ARKKGKGLHK DVVIFWHKKK ISPSKKLFPL KEKLGESEAS QGIEDSPFQH SPLSKPIVRP 

       550        560        570        580        590        600 
AAGSLLSKSS PVKKPSLLKM HQFEADPFDS GSEGSEGLGS CVSSLSTLIG TVADTSEEKY 

       610        620        630        640        650        660 
RLLDQRDRIM RQARVKRTDL DKARAFVGTC PDMCPEKERY LRETRSQLSV FEVVPGTDQV 

       670        680        690        700        710        720 
DHAAAVKEYS RSSADQEEPL PHELRPSAVL SRTMDYLVTQ IMDQKEGSLR DWYDFVWNRT 

       730        740        750        760        770        780 
RGIRKDITQQ HLCDPLTVSL IEKCTRFHIH CAHFMCEEPM SSFDAKINNE NMTKCLQSLK 

       790        800        810        820        830        840 
EMYQDLRNKG VFCASEAEFQ GYNVLLNLNK GDILREVQQF HPDVRNSPEV NFAVQAFAAL 

       850        860        870        880        890        900 
NSNNFVRFFK LVQSASYLNA CLLHCYFNQI RKDALRALNV AYTVSTQRST VFPLDGVVRM 

       910        920        930        940        950        960 
LLFRDSEEAT NFLNYHGLTV ADGCVELNRS AFLEPEGLCK ARKSVFIGRK LTVSVGEVVN 

       970        980        990       1000       1010       1020 
GGPLPPVPRH TPVCSFNSQN KYVGESLATE LPISTQRAGG DPAGGGRGED CEAEVDVPTL 

      1030       1040       1050       1060       1070       1080 
AVLPQPPPAS SATPALHVQP LAPAAAPSLL QASTQPEVLL PKPAPVYSDS DLVQVVDELI 

      1090       1100       1110       1120       1130       1140 
QEALQVDCEE VSSAGAAYVA AALGVSNAAV EDLITAATTG ILRHVAAEEV SMERQRLEEE 

      1150       1160       1170       1180       1190       1200 
KQRAEEERLK QERELMLTQL SEGLAAELTE LTVTECVWET CSQELQSAVE IDQKVRVARC 

      1210       1220       1230       1240       1250       1260 
CEAVCAHLVD LFLAEEIFQT AKETLQELQC FCKYLQRWRE AVAARKKFRR QMRAFPAAPC 

      1270       1280       1290       1300       1310       1320 
CVDVNDRLQA LVPSAECPIT EENLAKGLLD LGHAGKVGVS CTRLRRLRNK TAHQIKVQHF 

      1330       1340       1350       1360       1370       1380 
HQQLLRNAAW APLDLPSIVS EHLPMKQKRR FWKLVLVLPD VEEQTPESPG RILENWLKVK 

      1390       1400       1410       1420       1430       1440 
FTGDDSMVGD IGDNAGDIQT LSVFNTLSSK GDQTVSVNVC IKVAHGTLSD SALDAVETQK 

      1450       1460       1470       1480       1490       1500 
DLLGTSGLML LLPPKVKSEE VAEEELSWLS ALLQLKQLLQ AKPFQPALPL VVLVPSSRGD 

      1510       1520       1530       1540       1550       1560 
SAGRAVEDGL MLQDLVSAKL ISDYIVVEIP DSVNDLQGTV KVSGAVQWLI SRCPQALDLC 

      1570       1580       1590       1600       1610       1620 
CQTLVQYVED GISREFSRRF FHDRRERRLA SLPSQEPSTI IELFNSVLQF LASVVSSEQL 

      1630       1640       1650       1660       1670       1680 
CDISWPVMEF AEVGGSQLLP HLHWNSPEHL AWLKQAVLGF QLPQMDLPPP GAPWLPVCSM 

      1690       1700       1710       1720       1730       1740 
VIQYTSQIPS SSQTQPVLQS QVENLLCRTY QKWKNKSLSP GQELGPSVAE IPWDDIITLC 

      1750       1760       1770       1780       1790       1800 
INHKLRDWTP PRLPVTLEAL SEDGQICVYF FKNLLRKYHV PLSWEQARMQ TQRELQLSHG 

      1810       1820       1830       1840       1850       1860 
RSGMRSIHPP TSTFPTPLLH VHQKGKKKEE SGREGSLSTE DLLRGASAEE LLAQSLSSSL 

      1870       1880       1890       1900       1910       1920 
LEEKEENKRF EDQLQQWLSQ DSQAFTESTR LPLYLPQTLV SFPDSIKTQT MVKTSTSPQN 

      1930       1940       1950       1960       1970 
SGTGKQLRFS EASGSSLTEK LKLLERLIQS SRAEEAASEL HLSALLEMVD M 

« Hide

References

« Hide 'large scale' references
[1]"A novel nuclear phosphoprotein, GANP, is up-regulated in centrocytes of the germinal center and associated with MCM3, a protein essential for DNA replication."
Kuwahara K., Yoshida M., Kondo E., Sakata A., Watanabe Y., Abe E., Kouno Y., Tomiyasu S., Fujimura S., Tokuhisa T., Kimura H., Ezaki T., Sakaguchi N.
Blood 95:2321-2328(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH MCM3, TISSUE SPECIFICITY.
Strain: BALB/c X NZB.
[2]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[4]"Germinal center-associated nuclear protein (GANP) has a phosphorylation-dependent DNA-primase activity that is up-regulated in germinal center regions."
Kuwahara K., Tomiyasu S., Fujimura S., Nomura K., Xing Y., Nishiyama N., Ogawa M., Imajoh-Ohmi S., Izuta S., Sakaguchi N.
Proc. Natl. Acad. Sci. U.S.A. 98:10279-10283(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PHOSPHORYLATION AT SER-502, MUTAGENESIS OF SER-502.
[5]"GANP suppresses DNA recombination, measured by direct-repeat beta-galactosidase gene construct, but does not suppress the type of recombination applying to immunoglobulin genes in mammalian cells."
Yoshida M., Kuwahara K., Shimasaki T., Nakagata N., Matsuoka M., Sakaguchi N.
Genes Cells 12:1205-1213(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[6]"Molecular mechanism of immunoglobulin V-region diversification regulated by transcription and RNA metabolism in antigen-driven B cells."
Sakaguchi N., Maeda K., Kuwahara K.
Scand. J. Immunol. 73:520-526(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH AICDA, SUBCELLULAR LOCATION.
[7]"Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-483 AND LYS-484, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ006590 mRNA. Translation: CAB44241.1.
CH466553 Genomic DNA. Translation: EDL31849.1.
BC052452 mRNA. Translation: AAH52452.1.
CCDSCCDS23947.1.
RefSeqNP_062307.2. NM_019434.2.
UniGeneMm.30098.

3D structure databases

ProteinModelPortalQ9WUU9.
SMRQ9WUU9. Positions 1159-1227.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9WUU9. 1 interaction.
MINTMINT-4101590.

PTM databases

PhosphoSiteQ9WUU9.

Proteomic databases

PaxDbQ9WUU9.
PRIDEQ9WUU9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000170795; ENSMUSP00000125960; ENSMUSG00000001150.
GeneID54387.
KEGGmmu:54387.
UCSCuc007fup.1. mouse.

Organism-specific databases

CTD8888.
MGIMGI:1930089. Mcm3ap.

Phylogenomic databases

eggNOGCOG5079.
GeneTreeENSGT00530000063781.
HOVERGENHBG052431.
InParanoidQ7TS87.
OMAAHQMKVQ.
OrthoDBEOG78D7JB.
TreeFamTF105948.

Gene expression databases

BgeeQ9WUU9.
GenevestigatorQ9WUU9.

Family and domain databases

InterProIPR005062. SAC3/GANP/Nin1/mts3/eIF-3-p25.
[Graphical view]
PfamPF03399. SAC3_GANP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio311220.
PROQ9WUU9.
SOURCESearch...

Entry information

Entry nameGANP_MOUSE
AccessionPrimary (citable) accession number: Q9WUU9
Secondary accession number(s): Q7TS87
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot