Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

TNFAIP3-interacting protein 1

Gene

Tnip1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits NF-kappa-B activation and TNF-induced NF-kappa-B-dependent gene expression by regulating A20/TNFAIP3-mediated deubiquitination of IKBKG; proposed to link A20/TNFAIP3 to ubiquitinated IKBKG. Involved in regulation of EGF-induced ERK1/ERK2 signaling pathway; blocks MAPK3/MAPK1 nuclear translocation and MAPK1-dependent transcription. Increases cell surface CD4(T4) antigen expression. Involved in the anti-inflammatory response of macrophages and positively regulates TLR-induced activation of CEBPB. Involved in the prevention of autoimmunity; this function implicates binding to polyubiquitin. Involved in leukocyte integrin activation during inflammation; this function is mediated by association with SELPLG and dependent on phosphorylation by SRC-family kinases.3 Publications

GO - Molecular functioni

  • mitogen-activated protein kinase binding Source: UniProtKB
  • polyubiquitin binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Inflammatory response

Names & Taxonomyi

Protein namesi
Recommended name:
TNFAIP3-interacting protein 1
Alternative name(s):
A20-binding inhibitor of NF-kappa-B activation 1
Short name:
ABIN
Short name:
ABIN-1
Nef-associated factor 1
Short name:
Naf1
Virion-associated nuclear shuttling protein
Short name:
VAN
Short name:
mVAN
Gene namesi
Name:Tnip1
Synonyms:Abin, Naf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1926194. Tnip1.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner.By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryonic lethal. Mice develop an inflammatory disease with characteristics of human systemic lupus erythematosus (SLE), including the appearance of immature granulocytes in the peripheral blood and development of autoreactive antibodies and glomerulonephrititis.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi477 – 4782QQ → EE: Loss of inhibitory activity on TNF-induced NF-kappa-B activation; no effect on interaction with TNFAIP3. 1 Publication
Mutagenesisi485 – 4862DF → NA: Abolishes ubiquitin binding; loss of inhibitory activity on TNF-induced NF-kappa-B activation; no effect on interaction with TNFAIP3. 2 Publications
Mutagenesisi485 – 4851D → N: Abolishes interaction with polyubiquitinated IRAK1. 1 Publication
Mutagenesisi489 – 4902ER → AA: Abolishes ubiquitin binding; loss of inhibitory activity on NF-kappa-B activation. 1 Publication
Mutagenesisi493 – 4942RE → AQ: Loss of inhibitory activity on TNF-induced NF-kappa-B activation; no effect on interaction with TNFAIP3. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 647647TNFAIP3-interacting protein 1PRO_0000096692Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei77 – 771PhosphoserineBy similarity
Modified residuei297 – 2971PhosphoserineBy similarity
Modified residuei416 – 4161PhosphoserineBy similarity
Modified residuei455 – 4551PhosphoserineBy similarity
Modified residuei565 – 5651PhosphotyrosineBy similarity
Modified residuei638 – 6381PhosphoserineBy similarity

Post-translational modificationi

Phosphorylation at Tyr-565 by SRC-family kinases recruits phosphoinositide-3-kinase (PI3K) PIK3CD:p85 heterodimer which results in integrin activation and leukocyte adhesion to activated endothelium during inflammation.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9WUU8.
PaxDbiQ9WUU8.
PRIDEiQ9WUU8.

PTM databases

iPTMnetiQ9WUU8.
PhosphoSiteiQ9WUU8.

Expressioni

Tissue specificityi

Ubiquitous. Abundant in heart and skeletal muscle and expressed at lower levels in thymus, liver, kidney, brain and intestinal tract.

Gene expression databases

BgeeiENSMUSG00000020400.
CleanExiMM_NAF1.
MM_TNIP1.
ExpressionAtlasiQ9WUU8. baseline and differential.
GenevisibleiQ9WUU8. MM.

Interactioni

Subunit structurei

Interacts with TNFAIP3 and IKBKG (polyubiquitinated); facilitates TNFAIP3-mediated de-ubiquitination of NEMO/IKBKG. Interacts with polyubiquitin. Interacts with MAPK1, SELPLG and PIK3CD. Interacts with IRAK1 (polyubiquitinated). Interacts with MYD88; the interaction is indicative for participation in an activated TLR-signaling complex.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Tnfaip3Q607693EBI-6126152,EBI-646595

GO - Molecular functioni

  • mitogen-activated protein kinase binding Source: UniProtKB
  • polyubiquitin binding Source: UniProtKB

Protein-protein interaction databases

DIPiDIP-59199N.
IntActiQ9WUU8. 3 interactions.
STRINGi10090.ENSMUSP00000018482.

Structurei

3D structure databases

ProteinModelPortaliQ9WUU8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni95 – 425331Interacts with NefAdd
BLAST
Regioni444 – 601158Required for inhibitory activity of TNF-induced NF-kappa-B activationAdd
BLAST
Regioni465 – 52359Ubiquitin-binding domain (UBD)Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili39 – 7234Sequence analysisAdd
BLAST
Coiled coili209 – 27062Sequence analysisAdd
BLAST
Coiled coili311 – 551241Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi537 – 5437Nuclear localization signalSequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi162 – 1654Poly-Pro
Compositional biasi552 – 64796Pro-richAdd
BLAST

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IEGE. Eukaryota.
ENOG411180D. LUCA.
GeneTreeiENSGT00510000046908.
HOGENOMiHOG000253048.
HOVERGENiHBG019072.
InParanoidiQ9WUU8.
PhylomeDBiQ9WUU8.

Family and domain databases

InterProiIPR033372. TNIP1.
[Graphical view]
PANTHERiPTHR31882:SF3. PTHR31882:SF3. 2 hits.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9WUU8-1) [UniParc]FASTAAdd to basket
Also known as: ABINl

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGRGPYRIY DPGGSTPLGE VSAAFERLVE ENTRLKGKMQ GIKMLGELLE
60 70 80 90 100
ESQMEASRLR QKAEELVKDS ELSPPTSAPS LVSFDDLAEL TGQDTKVQVH
110 120 130 140 150
PATSTAATTT ATATTGNSME KPEPASKSPS NGASSDFEVV PTEEQNSPET
160 170 180 190 200
GSHPTNMMDL GPPPPEDSNL KLHLQRLETT LSVCAEEPDH SQLFTHLGRM
210 220 230 240 250
ALEFNRLASK VHKNEQRTSI LQTLCEQLRQ ENEALKAKLD KGLEQRDLAA
260 270 280 290 300
ERLREENTEL KKLLMNSSCK EGLCGQPSSP KPEGAGKKGV AGQQQASVMA
310 320 330 340 350
SKVPEAGAFG AAEKKVKLLE QQRMELLEVN KQWDQHFRSM KQQYEQKITE
360 370 380 390 400
LRQKLVDLQK QVTELEAERE QKQRDFDRKL LLAKSKIEME ETDKEQLTAE
410 420 430 440 450
AKELRQKVRY LQDQLSPLTR QREYQEKEIQ RLNKALEEAL SIQASPSSPP
460 470 480 490 500
AAFGSPEGVG GHLRKQELVT QNELLKQQVK IFEEDFQRER SDRERMNEEK
510 520 530 540 550
EELKKQVEKL QAQVTLTNAQ LKTLKEEEKA KEALKQQKRK AKASGERYHM
560 570 580 590 600
EPHPEHVCGA YPYAYPPMPA MVPHHAYKDW SQIRYPPPPV PMEHPPPHPN
610 620 630 640
SRLFHLPEYT WRPPCAGIRN QSSQVMDPPP DRPAEPESAD NDCDGPQ
Length:647
Mass (Da):73,050
Last modified:November 1, 1999 - v1
Checksum:i4280879A8C24A16E
GO
Isoform 2 (identifier: Q9WUU8-2) [UniParc]FASTAAdd to basket
Also known as: ABINs

The sequence of this isoform differs from the canonical sequence as follows:
     1-53: Missing.

Show »
Length:594
Mass (Da):67,199
Checksum:i9ADB57CC9A90DDCF
GO
Isoform 3 (identifier: Q9WUU8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     638-647: SADNDCDGPQ → PADLRLPKV

Show »
Length:646
Mass (Da):73,038
Checksum:iE6E3C745AA020E23
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti97 – 982VQ → TR in AAG42155 (PubMed:11090181).Curated
Sequence conflicti308 – 3081A → V in AAH08186 (PubMed:15489334).Curated
Sequence conflicti533 – 5331A → V in AAH08186 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5353Missing in isoform 2. 1 PublicationVSP_003914Add
BLAST
Alternative sequencei638 – 64710SADNDCDGPQ → PADLRLPKV in isoform 3. 2 PublicationsVSP_003915

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ242777 mRNA. Translation: CAB44239.1.
AJ242778 mRNA. Translation: CAB44240.1.
BC008186 mRNA. Translation: AAH08186.1.
BC008665 mRNA. Translation: AAH08665.1.
AX011241 Unassigned DNA. Translation: CAC07546.1.
AY012159 mRNA. Translation: AAG42155.2.
CCDSiCCDS24704.1. [Q9WUU8-1]
CCDS56769.1. [Q9WUU8-2]
CCDS70188.1. [Q9WUU8-3]
RefSeqiNP_001186205.1. NM_001199276.2. [Q9WUU8-2]
NP_001258384.1. NM_001271455.1. [Q9WUU8-2]
XP_006533910.1. XM_006533847.2. [Q9WUU8-2]
XP_011247465.1. XM_011249163.1. [Q9WUU8-2]
UniGeneiMm.259671.

Genome annotation databases

EnsembliENSMUST00000108885; ENSMUSP00000104513; ENSMUSG00000020400. [Q9WUU8-2]
ENSMUST00000108886; ENSMUSP00000104514; ENSMUSG00000020400. [Q9WUU8-2]
GeneIDi57783.
KEGGimmu:57783.
UCSCiuc007iyn.2. mouse. [Q9WUU8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ242777 mRNA. Translation: CAB44239.1.
AJ242778 mRNA. Translation: CAB44240.1.
BC008186 mRNA. Translation: AAH08186.1.
BC008665 mRNA. Translation: AAH08665.1.
AX011241 Unassigned DNA. Translation: CAC07546.1.
AY012159 mRNA. Translation: AAG42155.2.
CCDSiCCDS24704.1. [Q9WUU8-1]
CCDS56769.1. [Q9WUU8-2]
CCDS70188.1. [Q9WUU8-3]
RefSeqiNP_001186205.1. NM_001199276.2. [Q9WUU8-2]
NP_001258384.1. NM_001271455.1. [Q9WUU8-2]
XP_006533910.1. XM_006533847.2. [Q9WUU8-2]
XP_011247465.1. XM_011249163.1. [Q9WUU8-2]
UniGeneiMm.259671.

3D structure databases

ProteinModelPortaliQ9WUU8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59199N.
IntActiQ9WUU8. 3 interactions.
STRINGi10090.ENSMUSP00000018482.

PTM databases

iPTMnetiQ9WUU8.
PhosphoSiteiQ9WUU8.

Proteomic databases

MaxQBiQ9WUU8.
PaxDbiQ9WUU8.
PRIDEiQ9WUU8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000108885; ENSMUSP00000104513; ENSMUSG00000020400. [Q9WUU8-2]
ENSMUST00000108886; ENSMUSP00000104514; ENSMUSG00000020400. [Q9WUU8-2]
GeneIDi57783.
KEGGimmu:57783.
UCSCiuc007iyn.2. mouse. [Q9WUU8-1]

Organism-specific databases

CTDi10318.
MGIiMGI:1926194. Tnip1.

Phylogenomic databases

eggNOGiENOG410IEGE. Eukaryota.
ENOG411180D. LUCA.
GeneTreeiENSGT00510000046908.
HOGENOMiHOG000253048.
HOVERGENiHBG019072.
InParanoidiQ9WUU8.
PhylomeDBiQ9WUU8.

Miscellaneous databases

PROiQ9WUU8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020400.
CleanExiMM_NAF1.
MM_TNIP1.
ExpressionAtlasiQ9WUU8. baseline and differential.
GenevisibleiQ9WUU8. MM.

Family and domain databases

InterProiIPR033372. TNIP1.
[Graphical view]
PANTHERiPTHR31882:SF3. PTHR31882:SF3. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiTNIP1_MOUSE
AccessioniPrimary (citable) accession number: Q9WUU8
Secondary accession number(s): Q922A9
, Q922F7, Q9EPP8, Q9R0X3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: November 1, 1999
Last modified: September 7, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.