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Protein

Gap junction alpha-10 protein

Gene

Gja10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Involved in tracer coupling between horizontal cells of the retina. May play a role in the regulation of horizontal cell patterning.4 Publications

GO - Molecular functioni

  • gap junction channel activity Source: MGI

GO - Biological processi

  • cell communication Source: InterPro
  • detection of light stimulus involved in visual perception Source: MGI
  • gamete generation Source: MGI
  • response to light stimulus Source: MGI
  • synapse assembly Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-112303. Electric Transmission Across Gap Junctions.

Names & Taxonomyi

Protein namesi
Recommended name:
Gap junction alpha-10 protein
Alternative name(s):
Connexin-57
Short name:
Cx57
Gene namesi
Name:Gja10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1339969. Gja10.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1616CytoplasmicSequence analysisAdd
BLAST
Transmembranei17 – 3721HelicalSequence analysisAdd
BLAST
Topological domaini38 – 7639ExtracellularSequence analysisAdd
BLAST
Transmembranei77 – 9721HelicalSequence analysisAdd
BLAST
Topological domaini98 – 16568CytoplasmicSequence analysisAdd
BLAST
Transmembranei166 – 18621HelicalSequence analysisAdd
BLAST
Topological domaini187 – 20923ExtracellularSequence analysisAdd
BLAST
Transmembranei210 – 23021HelicalSequence analysisAdd
BLAST
Topological domaini231 – 505275CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • connexon complex Source: InterPro
  • gap junction Source: MGI
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Gap junction, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are fertile and show no obvious anatomical or behavioral abnormalities. In horizontal cells of the retina tracer coupling is abolished but the spatial tuning of ganglion cells is unchanged.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 505505Gap junction alpha-10 proteinPRO_0000057841Add
BLAST

Proteomic databases

PaxDbiQ9WUS4.
PRIDEiQ9WUS4.

Expressioni

Tissue specificityi

Low levels were detected in skin, heart, kidney, testis, ovary, intestine. Expression not detected in brain, sciatic nerve or liver. According to PubMed:15147297 expression is detected only in horizontal cells in the inner nuclear layer of the retina and not in other neurons of the central nervous system or tissues. Detected in the outer plexiform layer of the retina (at protein level).3 Publications

Developmental stagei

Expressed in the retina from E16.5 to P1, in the thymus from E18.5 to P1, and in kidney from E16.5 to E18.5.1 Publication

Gene expression databases

BgeeiQ9WUS4.
CleanExiMM_GJA10.
GenevisibleiQ9WUS4. MM.

Interactioni

Subunit structurei

A connexon is composed of a hexamer of connexins.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000061742.

Structurei

3D structure databases

ProteinModelPortaliQ9WUS4.
SMRiQ9WUS4. Positions 3-236.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IGQJ. Eukaryota.
ENOG4110KSA. LUCA.
GeneTreeiENSGT00840000129674.
HOGENOMiHOG000231126.
HOVERGENiHBG009576.
InParanoidiQ9WUS4.
KOiK07631.
OMAiEVHSHST.
OrthoDBiEOG7P2XSS.
PhylomeDBiQ9WUS4.
TreeFamiTF329606.

Family and domain databases

InterProiIPR000500. Connexin.
IPR019570. Connexin_CCC.
IPR017990. Connexin_CS.
IPR013092. Connexin_N.
[Graphical view]
PANTHERiPTHR11984. PTHR11984. 1 hit.
PfamiPF00029. Connexin. 1 hit.
[Graphical view]
PRINTSiPR00206. CONNEXIN.
SMARTiSM00037. CNX. 1 hit.
SM01089. Connexin_CCC. 1 hit.
[Graphical view]
PROSITEiPS00407. CONNEXINS_1. 1 hit.
PS00408. CONNEXINS_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9WUS4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDWNLLGGI LEEVHSHSTI VGKIWLTILF IFRMLVLGVA AEDVWDDEQS
60 70 80 90 100
AFACNTQQPG CNNICYDDAF PISLIRFWVL QIIFVSSPSL VYMGHALYRL
110 120 130 140 150
RDFEKQRQKK KLYLRAQMEN PELDLEEQQR VDKELRRLEE QKRIHKVPLK
160 170 180 190 200
GCLLRTYVLH ILTRSVLEVG FMIGQYILYG FQMHPIYKCT QAPCPNSVDC
210 220 230 240 250
FVSRPTEKTI FMLFMHSIAA ISLLLNILEI FHLGIRKIMR ALDGKSSSGN
260 270 280 290 300
TENETGPPFH STNYSGTQQC MICSSLPERI SLLQANNKQQ VIRVNIPRSK
310 320 330 340 350
SMWQIPHPRQ LEVDVSCGKR DWAEKIESCA QLHVHSPCPH DRSARIQHPG
360 370 380 390 400
QQPCHSVFGP KNAMSQSWFG TMTASQHRPS SALETWERSQ GPEASGRSLT
410 420 430 440 450
DRQSHFQGSD GSARESGVWT DRLGPGSRKA SFLSRLMSEK GQRHSDSGSS
460 470 480 490 500
RSLNSSCLDF SHGENSPSPL PSATGHRASM VSKSSHVDSP PHSSFIIHET

YVYVY
Length:505
Mass (Da):57,127
Last modified:January 15, 2008 - v2
Checksum:iF7FAFBA21EDE2A7B
GO
Isoform 2 (identifier: Q9WUS4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     481-505: VSKSSHVDSPPHSSFIIHETYVYVY → NMLLELSSIMKK

Note: No experimental confirmation available.
Show »
Length:492
Mass (Da):55,655
Checksum:i7E29240C66717170
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti143 – 1431R → K in CAB40358 (PubMed:10329667).Curated
Sequence conflicti272 – 2721I → V in CAB40358 (PubMed:10329667).Curated
Sequence conflicti330 – 3301A → T in CAB40358 (PubMed:10329667).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei481 – 50525VSKSS…YVYVY → NMLLELSSIMKK in isoform 2. 1 PublicationVSP_035809Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010741 Genomic DNA. Translation: CAB40358.1.
AL831746 Genomic DNA. Translation: CAM26624.1.
CCDSiCCDS18015.1. [Q9WUS4-1]
RefSeqiNP_034419.2. NM_010289.2. [Q9WUS4-1]
UniGeneiMm.377084.

Genome annotation databases

EnsembliENSMUST00000056517; ENSMUSP00000061742; ENSMUSG00000051056. [Q9WUS4-1]
GeneIDi14610.
KEGGimmu:14610.
UCSCiuc008sev.1. mouse. [Q9WUS4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010741 Genomic DNA. Translation: CAB40358.1.
AL831746 Genomic DNA. Translation: CAM26624.1.
CCDSiCCDS18015.1. [Q9WUS4-1]
RefSeqiNP_034419.2. NM_010289.2. [Q9WUS4-1]
UniGeneiMm.377084.

3D structure databases

ProteinModelPortaliQ9WUS4.
SMRiQ9WUS4. Positions 3-236.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000061742.

Proteomic databases

PaxDbiQ9WUS4.
PRIDEiQ9WUS4.

Protocols and materials databases

DNASUi14610.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000056517; ENSMUSP00000061742; ENSMUSG00000051056. [Q9WUS4-1]
GeneIDi14610.
KEGGimmu:14610.
UCSCiuc008sev.1. mouse. [Q9WUS4-1]

Organism-specific databases

CTDi84694.
MGIiMGI:1339969. Gja10.

Phylogenomic databases

eggNOGiENOG410IGQJ. Eukaryota.
ENOG4110KSA. LUCA.
GeneTreeiENSGT00840000129674.
HOGENOMiHOG000231126.
HOVERGENiHBG009576.
InParanoidiQ9WUS4.
KOiK07631.
OMAiEVHSHST.
OrthoDBiEOG7P2XSS.
PhylomeDBiQ9WUS4.
TreeFamiTF329606.

Enzyme and pathway databases

ReactomeiR-MMU-112303. Electric Transmission Across Gap Junctions.

Miscellaneous databases

PROiQ9WUS4.
SOURCEiSearch...

Gene expression databases

BgeeiQ9WUS4.
CleanExiMM_GJA10.
GenevisibleiQ9WUS4. MM.

Family and domain databases

InterProiIPR000500. Connexin.
IPR019570. Connexin_CCC.
IPR017990. Connexin_CS.
IPR013092. Connexin_N.
[Graphical view]
PANTHERiPTHR11984. PTHR11984. 1 hit.
PfamiPF00029. Connexin. 1 hit.
[Graphical view]
PRINTSiPR00206. CONNEXIN.
SMARTiSM00037. CNX. 1 hit.
SM01089. Connexin_CCC. 1 hit.
[Graphical view]
PROSITEiPS00407. CONNEXINS_1. 1 hit.
PS00408. CONNEXINS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and functional expression of the mouse gap junction gene connexin-57 in human HeLa cells."
    Manthey D., Bukauskas F., Kozak C., Willecke K., Lee C.G.
    J. Biol. Chem. 274:14716-14723(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "Functional expression of connexin57 in horizontal cells of the mouse retina."
    Hombach S., Janssen-Bienhold U., Soehl G., Schubert T., Buessow H., Ott T., Weiler R., Willecke K.
    Eur. J. Neurosci. 19:2633-2640(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 478-505 (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
  4. "Expression profiles of the novel human connexin genes hCx30.2, hCx40.1, and hCx62 differ from their putative mouse orthologues."
    Soehl G., Nielsen P.A., Eiberger J., Willecke K.
    Cell Commun. Adhes. 10:27-36(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. "Horizontal cell receptive fields are reduced in connexin57-deficient mice."
    Shelley J., Dedek K., Schubert T., Feigenspan A., Schultz K., Hombach S., Willecke K., Weiler R.
    Eur. J. Neurosci. 23:3176-3186(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  6. "Spatial relationships of connexin36, connexin57 and zonula occludens-1 in the outer plexiform layer of mouse retina."
    Ciolofan C., Lynn B.D., Wellershaus K., Willecke K., Nagy J.I.
    Neuroscience 148:473-488(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  7. "Ganglion cell adaptability: does the coupling of horizontal cells play a role?"
    Dedek K., Pandarinath C., Alam N.M., Wellershaus K., Schubert T., Willecke K., Prusky G.T., Weiler R., Nirenberg S.
    PLoS ONE 3:E1714-E1714(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiCXA10_MOUSE
AccessioniPrimary (citable) accession number: Q9WUS4
Secondary accession number(s): A2ANV0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: January 15, 2008
Last modified: July 6, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.