Q9WUS1 (RNAS1_GLIGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease pancreatic
EC=3.1.27.5

Alternative name(s):
RNase 1
RNase A

Gene names

Name:

RNASE1

Organism

Glis glis (Fat dormouse) (Myoxus glis)

Taxonomic identifier

41261 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Gliridae › Glirinae › Glis

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA By similarity.
Catalytic activity	Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.
Subunit structure	Monomer. Interacts with and forms tight 1:1 complexes with RNH1. Dimerization of two such complexes may occur. Interaction with RNH1 inhibits this protein By similarity.
Subcellular location	Secreted.
Tissue specificity	Pancreas.
Sequence similarities	Belongs to the pancreatic ribonuclease family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro pancreatic ribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 156	130	Ribonuclease pancreatic		PRO_0000030932
Regions
	Region	69 – 73	5	Substrate binding By similarity
Sites
	Active site	38	1	Proton acceptor By similarity
	Active site	147	1	Proton donor By similarity
	Binding site	33	1	Substrate By similarity
	Binding site	36	1	Substrate By similarity
	Binding site	94	1	Substrate By similarity
	Binding site	113	1	Substrate By similarity
Amino acid modifications
	Glycosylation	90	1	N-linked (GlcNAc...) Potential
	Disulfide bond	54 ↔ 112		By similarity
	Disulfide bond	68 ↔ 123		By similarity
	Disulfide bond	86 ↔ 138		By similarity
	Disulfide bond	93 ↔ 100		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9WUS1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 58B0D7793EAC082E	FASTA	156	17,353
	10 20 30 40 50 60 MGLEKSLVFF PLLVLLALGW VQPCLGRESS AHKFRRQHMD SEGLSSSSSS PTYCNQMMQR 70 80 90 100 110 120 RKLTRGHCKP VNTFVHEPLA DVQAVCFQEN VTCKNGQTNC YRSGSSMHVT DCRTTGSSKY 130 140 150 PNCAYRTTQK VKRIVVACEG DPSVPVHYDG SVEDST

References

[1]	"The phylogenetic position of 'Acomyinae' (Rodentia, Mammalia) as sister group of a Murinae + Gerbillinae clade: evidence from the nuclear ribonuclease gene." Dubois J.-Y.F., Catzeflis F.M., Beintema J.J. Mol. Phylogenet. Evol. 13:181-192(1999) [PubMed: 10508551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ005767 Genomic DNA. Translation: CAB41478.1.
3D structure databases
ProteinModelPortal	Q9WUS1.
SMR	Q9WUS1. Positions 27-153.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG008396.
Family and domain databases
InterPro	IPR001427. RNaseA. IPR023411. RNaseA_AS. IPR023412. RNaseA_domain. [Graphical view]
Gene3D	G3DSA:3.10.130.10. RNaseA. 1 hit.
PANTHER	PTHR11437. RNaseA. 1 hit.
Pfam	PF00074. RnaseA. 1 hit. [Graphical view]
PRINTS	PR00794. RIBONUCLEASE.
ProDom	PD000535. RNaseA. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00092. RNAse_Pc. 1 hit. [Graphical view]
SUPFAM	SSF54076. RNaseA. 1 hit.
PROSITE	PS00127. RNASE_PANCREATIC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNAS1_GLIGL

Accession

Primary (citable) accession number: Q9WUS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	November 1, 1999
Last modified:	June 28, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program