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Protein

Adenylate kinase 4, mitochondrial

Gene

Ak4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Efficiently phosphorylates AMP and dAMP using ATP as phosphate donor, but phosphorylates only AMP when using GTP as phosphate donor. Also displays broad nucleoside diphosphate kinase activity.UniRule annotation

Catalytic activityi

NTP + AMP = NDP + ADP.UniRule annotation
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36AMPUniRule annotation1
Binding sitei41AMPUniRule annotation1
Binding sitei96AMPUniRule annotation1
Binding sitei126NTPUniRule annotation1
Binding sitei170AMPUniRule annotation1
Binding sitei199NTP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 20NTPUniRule annotation6
Nucleotide bindingi62 – 64AMPUniRule annotation3
Nucleotide bindingi89 – 92AMPUniRule annotation4
Nucleotide bindingi135 – 136NTPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

  • AMP metabolic process Source: Ensembl
  • ATP metabolic process Source: Ensembl
  • brain development Source: RGD
  • GTP metabolic process Source: Ensembl
  • liver development Source: RGD
  • nucleoside diphosphate phosphorylation Source: UniProtKB
  • nucleoside triphosphate biosynthetic process Source: UniProtKB
  • response to drug Source: RGD

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-499943 Interconversion of nucleotide di- and triphosphates

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate kinase 4, mitochondrialUniRule annotation (EC:2.7.4.10UniRule annotation, EC:2.7.4.6UniRule annotation)
Short name:
AK 4UniRule annotation
Alternative name(s):
Adenylate kinase 3-likeUniRule annotation
Adenylate kinase isoenzyme 4
GTP:AMP phosphotransferase AK4UniRule annotation
Gene namesi
Name:Ak4
Synonyms:Ak3l1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi2078 Ak4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001589291 – 223Adenylate kinase 4, mitochondrialAdd BLAST223

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei60N6-succinyllysineBy similarity1
Modified residuei175N6-acetyllysineBy similarity1
Modified residuei179N6-acetyllysine; alternateBy similarity1
Modified residuei179N6-succinyllysine; alternateBy similarity1
Modified residuei186N6-acetyllysine; alternateBy similarity1
Modified residuei186N6-succinyllysine; alternateBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9WUS0
PRIDEiQ9WUS0

PTM databases

iPTMnetiQ9WUS0
PhosphoSitePlusiQ9WUS0

Expressioni

Tissue specificityi

Expressed in the pyramidal cells in the hippocampus.

Developmental stagei

Expressed in the central nervous system in a region-specific manner from the middle stage of embryogenesis to the adulthood in the rodent.

Gene expression databases

BgeeiENSRNOG00000045738
GenevisibleiQ9WUS0 RN

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000065349

Structurei

3D structure databases

ProteinModelPortaliQ9WUS0
SMRiQ9WUS0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 64NMPbindUniRule annotationAdd BLAST30
Regioni125 – 162LIDUniRule annotationAdd BLAST38

Domaini

Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon GTP/ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent GTP/ATP hydrolysis.UniRule annotation

Sequence similaritiesi

Belongs to the adenylate kinase family. AK3 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG3078 Eukaryota
COG0563 LUCA
GeneTreeiENSGT00550000074679
HOVERGENiHBG000458
InParanoidiQ9WUS0
KOiK00939
OMAiGRVYNMG
OrthoDBiEOG091G06BH
PhylomeDBiQ9WUS0

Family and domain databases

CDDicd01428 ADK, 1 hit
HAMAPiMF_00235 Adenylate_kinase_Adk, 1 hit
MF_03169 Adenylate_kinase_AK3, 1 hit
MF_03170 Adenylate_kinase_AK4, 1 hit
InterProiView protein in InterPro
IPR006259 Adenyl_kin_sub
IPR000850 Adenylat/UMP-CMP_kin
IPR033690 Adenylat_kinase_CS
IPR007862 Adenylate_kinase_lid-dom
IPR028586 AK3/Ak4_mitochondrial
IPR028585 AK4_mitochondrial
IPR027417 P-loop_NTPase
PANTHERiPTHR23359 PTHR23359, 1 hit
PfamiView protein in Pfam
PF05191 ADK_lid, 1 hit
PRINTSiPR00094 ADENYLTKNASE
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01351 adk, 1 hit
PROSITEiView protein in PROSITE
PS00113 ADENYLATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q9WUS0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASKLLRAVI LGPPGSGKGT VCERIAQNFG LQHLSSGHLL RENLKTNTEV
60 70 80 90 100
GDVAKQYLEK GLLVPDHVIT RLMMSELETR SAQHWLLDGF PRTLVQAEAL
110 120 130 140 150
DRICDVDLVI SLNIPFETLK DRLSRRWIHP SSGRVYNLDF NPPQVLGVDD
160 170 180 190 200
ITGEPLVQQE DDKPEALAAR LRRYKDAAKP VIELYKSRGV LHQFSGTETN
210 220
RIWPYVYTLF SNKITPIQSK EAY
Length:223
Mass (Da):25,203
Last modified:November 1, 1999 - v1
Checksum:iB5A9BE45CFA3C19B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87809 mRNA Translation: BAA77761.1
BC087024 mRNA Translation: AAH87024.1
RefSeqiNP_058831.1, NM_017135.3
XP_017448685.1, XM_017593196.1
UniGeneiRn.1086

Genome annotation databases

EnsembliENSRNOT00000070967; ENSRNOP00000065349; ENSRNOG00000045738
GeneIDi29223
KEGGirno:29223
UCSCiRGD:2078 rat

Similar proteinsi

Entry informationi

Entry nameiKAD4_RAT
AccessioniPrimary (citable) accession number: Q9WUS0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: May 23, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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