Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Adenylate kinase 4, mitochondrial

Gene

Ak4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Efficiently phosphorylates AMP and dAMP using ATP as phosphate donor, but phosphorylates only AMP when using GTP as phosphate donor. Also displays broad nucleoside diphosphate kinase activity.UniRule annotation

Catalytic activityi

NTP + AMP = NDP + ADP.UniRule annotation
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361AMPUniRule annotation
Binding sitei41 – 411AMPUniRule annotation
Binding sitei96 – 961AMPUniRule annotation
Binding sitei126 – 1261NTPUniRule annotation
Binding sitei170 – 1701AMPUniRule annotation
Binding sitei199 – 1991NTP; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi15 – 206NTPUniRule annotation
Nucleotide bindingi62 – 643AMPUniRule annotation
Nucleotide bindingi89 – 924AMPUniRule annotation
Nucleotide bindingi135 – 1362NTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

  • ADP biosynthetic process Source: UniProtKB-HAMAP
  • AMP metabolic process Source: UniProtKB-HAMAP
  • ATP metabolic process Source: UniProtKB-HAMAP
  • brain development Source: RGD
  • GTP metabolic process Source: UniProtKB-HAMAP
  • liver development Source: RGD
  • nucleoside diphosphate phosphorylation Source: UniProtKB
  • nucleoside triphosphate biosynthetic process Source: UniProtKB
  • response to drug Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate kinase 4, mitochondrialUniRule annotation (EC:2.7.4.10UniRule annotation, EC:2.7.4.6UniRule annotation)
Short name:
AK 4UniRule annotation
Alternative name(s):
Adenylate kinase 3-likeUniRule annotation
Adenylate kinase isoenzyme 4
GTP:AMP phosphotransferase AK4UniRule annotation
Gene namesi
Name:Ak4
Synonyms:Ak3l1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi2078. Ak4.

Subcellular locationi

  • Mitochondrion matrix UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 223223Adenylate kinase 4, mitochondrialPRO_0000158929Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei60 – 601N6-succinyllysineBy similarity
Modified residuei175 – 1751N6-acetyllysineBy similarity
Modified residuei179 – 1791N6-acetyllysine; alternateBy similarity
Modified residuei179 – 1791N6-succinyllysine; alternateBy similarity
Modified residuei186 – 1861N6-acetyllysine; alternateBy similarity
Modified residuei186 – 1861N6-succinyllysine; alternateBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9WUS0.
PRIDEiQ9WUS0.

PTM databases

iPTMnetiQ9WUS0.

Expressioni

Tissue specificityi

Expressed in the pyramidal cells in the hippocampus.

Developmental stagei

Expressed in the central nervous system in a region-specific manner from the middle stage of embryogenesis to the adulthood in the rodent.

Gene expression databases

GenevisibleiQ9WUS0. RN.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000065349.

Structurei

3D structure databases

ProteinModelPortaliQ9WUS0.
SMRiQ9WUS0. Positions 4-223.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni35 – 6430NMPbindUniRule annotationAdd
BLAST
Regioni125 – 16238LIDUniRule annotationAdd
BLAST

Domaini

Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon GTP/ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent GTP/ATP hydrolysis.UniRule annotation

Sequence similaritiesi

Belongs to the adenylate kinase family. AK3 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG3078. Eukaryota.
COG0563. LUCA.
GeneTreeiENSGT00550000074679.
HOVERGENiHBG000458.
InParanoidiQ9WUS0.
KOiK00939.
OMAiIWPYINS.
OrthoDBiEOG74TX0R.
PhylomeDBiQ9WUS0.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00235. Adenylate_kinase_Adk.
MF_03169. Adenylate_kinase_AK3.
MF_03170. Adenylate_kinase_AK4.
InterProiIPR006259. Adenyl_kin_sub.
IPR000850. Adenylat/UMP-CMP_kin.
IPR007862. Adenylate_kinase_lid-dom.
IPR028586. AK3/Ak4_mitochondrial.
IPR028585. AK4_mitochondrial.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PfamiPF05191. ADK_lid. 1 hit.
[Graphical view]
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01351. adk. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WUS0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASKLLRAVI LGPPGSGKGT VCERIAQNFG LQHLSSGHLL RENLKTNTEV
60 70 80 90 100
GDVAKQYLEK GLLVPDHVIT RLMMSELETR SAQHWLLDGF PRTLVQAEAL
110 120 130 140 150
DRICDVDLVI SLNIPFETLK DRLSRRWIHP SSGRVYNLDF NPPQVLGVDD
160 170 180 190 200
ITGEPLVQQE DDKPEALAAR LRRYKDAAKP VIELYKSRGV LHQFSGTETN
210 220
RIWPYVYTLF SNKITPIQSK EAY
Length:223
Mass (Da):25,203
Last modified:November 1, 1999 - v1
Checksum:iB5A9BE45CFA3C19B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87809 mRNA. Translation: BAA77761.1.
BC087024 mRNA. Translation: AAH87024.1.
RefSeqiNP_058831.1. NM_017135.3.
UniGeneiRn.1086.

Genome annotation databases

EnsembliENSRNOT00000070967; ENSRNOP00000065349; ENSRNOG00000045738.
GeneIDi29223.
KEGGirno:29223.
UCSCiRGD:2078. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87809 mRNA. Translation: BAA77761.1.
BC087024 mRNA. Translation: AAH87024.1.
RefSeqiNP_058831.1. NM_017135.3.
UniGeneiRn.1086.

3D structure databases

ProteinModelPortaliQ9WUS0.
SMRiQ9WUS0. Positions 4-223.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000065349.

PTM databases

iPTMnetiQ9WUS0.

Proteomic databases

PaxDbiQ9WUS0.
PRIDEiQ9WUS0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000070967; ENSRNOP00000065349; ENSRNOG00000045738.
GeneIDi29223.
KEGGirno:29223.
UCSCiRGD:2078. rat.

Organism-specific databases

CTDi205.
RGDi2078. Ak4.

Phylogenomic databases

eggNOGiKOG3078. Eukaryota.
COG0563. LUCA.
GeneTreeiENSGT00550000074679.
HOVERGENiHBG000458.
InParanoidiQ9WUS0.
KOiK00939.
OMAiIWPYINS.
OrthoDBiEOG74TX0R.
PhylomeDBiQ9WUS0.

Enzyme and pathway databases

ReactomeiR-RNO-499943. Synthesis and interconversion of nucleotide di- and triphosphates.

Miscellaneous databases

NextBioi608427.
PROiQ9WUS0.

Gene expression databases

GenevisibleiQ9WUS0. RN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00235. Adenylate_kinase_Adk.
MF_03169. Adenylate_kinase_AK3.
MF_03170. Adenylate_kinase_AK4.
InterProiIPR006259. Adenyl_kin_sub.
IPR000850. Adenylat/UMP-CMP_kin.
IPR007862. Adenylate_kinase_lid-dom.
IPR028586. AK3/Ak4_mitochondrial.
IPR028585. AK4_mitochondrial.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PfamiPF05191. ADK_lid. 1 hit.
[Graphical view]
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01351. adk. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a novel adenylate kinase system in the brain: cloning of the fourth adenylate kinase."
    Yoneda T., Sato M., Maeda M., Takagi H.
    Brain Res. Mol. Brain Res. 62:187-195(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Forebrain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiKAD4_RAT
AccessioniPrimary (citable) accession number: Q9WUS0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: May 11, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.