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Protein

Enoyl-CoA delta isomerase 2, mitochondrial

Gene

Eci2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species (PubMed:24344334). Has a preference for 3-trans substrates (By similarity).By similarity1 Publication

Catalytic activityi

A (3Z)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA.1 Publication
A (3E)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei90Acyl-CoABy similarity1
Binding sitei109Acyl-CoABy similarity1
Sitei277Important for catalytic activityBy similarity1

GO - Molecular functioni

GO - Biological processi

  • fatty acid beta-oxidation Source: Ensembl
  • fatty acid catabolic process Source: UniProtKB

Keywordsi

Molecular functionIsomerase

Enzyme and pathway databases

ReactomeiR-MMU-390247 Beta-oxidation of very long chain fatty acids
R-MMU-9033241 Peroxisomal protein import

Chemistry databases

SwissLipidsiSLP:000001194

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-CoA delta isomerase 2, mitochondrial (EC:5.3.3.81 Publication)
Alternative name(s):
Delta(3),delta(2)-enoyl-CoA isomerase
Short name:
D3,D2-enoyl-CoA isomerase
Dodecenoyl-CoA isomerase
Peroxisomal 3,2-trans-enoyl-CoA isomerase
Short name:
pECI
Gene namesi
Name:Eci2
Synonyms:Peci
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1346064 Eci2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 36MitochondrionSequence analysisAdd BLAST36
ChainiPRO_000021402837 – 391Enoyl-CoA delta isomerase 2, mitochondrialAdd BLAST355

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei49N6-acetyllysine; alternateBy similarity1
Modified residuei49N6-succinyllysine; alternateCombined sources1
Modified residuei53N6-succinyllysineCombined sources1
Modified residuei60N6-acetyllysine; alternateCombined sources1
Modified residuei60N6-succinyllysine; alternateCombined sources1
Modified residuei68N6-succinyllysineCombined sources1
Modified residuei79N6-succinyllysineCombined sources1
Modified residuei88N6-succinyllysineCombined sources1
Modified residuei90N6-acetyllysine; alternateCombined sources1
Modified residuei90N6-succinyllysine; alternateCombined sources1
Modified residuei99PhosphoserineBy similarity1
Modified residuei117PhosphoserineBy similarity1
Modified residuei127N6-succinyllysineCombined sources1
Modified residuei159N6-succinyllysineCombined sources1
Modified residuei286N6-succinyllysineCombined sources1

Post-translational modificationi

Acetylation of Lys-60 is observed in liver mitochondria from fasted mice but not from fed mice.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9WUR2
PeptideAtlasiQ9WUR2
PRIDEiQ9WUR2

PTM databases

iPTMnetiQ9WUR2
PhosphoSitePlusiQ9WUR2
SwissPalmiQ9WUR2

Expressioni

Tissue specificityi

Expressed in liver and kidney (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000021417
ExpressionAtlasiQ9WUR2 baseline and differential
GenevisibleiQ9WUR2 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9WUR2, 2 interactors
STRINGi10090.ENSMUSP00000131735

Structurei

3D structure databases

ProteinModelPortaliQ9WUR2
SMRiQ9WUR2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 122ACBPROSITE-ProRule annotationAdd BLAST86

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni64 – 68Acyl-CoA bindingBy similarity5
Regioni149 – 319ECH-likeAdd BLAST171
Regioni196 – 200Substrate bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi389 – 391Microbody targeting signalSequence analysis3

Sequence similaritiesi

In the C-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0016 Eukaryota
KOG0817 Eukaryota
COG1024 LUCA
COG4281 LUCA
GeneTreeiENSGT00890000139344
HOVERGENiHBG006723
InParanoidiQ9WUR2
KOiK13239
OMAiGCFIDFP
OrthoDBiEOG091G0T5I
PhylomeDBiQ9WUR2
TreeFamiTF313375

Family and domain databases

Gene3Di1.10.12.10, 1 hit
1.20.80.10, 1 hit
InterProiView protein in InterPro
IPR022408 Acyl-CoA-binding_prot_CS
IPR000582 Acyl-CoA-binding_protein
IPR035984 Acyl-CoA-binding_sf
IPR029045 ClpP/crotonase-like_dom_sf
IPR001753 Enoyl-CoA_hydra/iso
IPR014748 enoyl-CoA_hydra_C
IPR014352 FERM/acyl-CoA-bd_prot_sf
PfamiView protein in Pfam
PF00887 ACBP, 1 hit
PF00378 ECH_1, 1 hit
PRINTSiPR00689 ACOABINDINGP
SUPFAMiSSF47027 SSF47027, 1 hit
SSF52096 SSF52096, 1 hit
PROSITEiView protein in PROSITE
PS00880 ACB_1, 1 hit
PS51228 ACB_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9WUR2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAVTWSRAR CWCPSVLQVF RLQVAKLHLG RPTMRASQQD FENALNQVKL
60 70 80 90 100
LKKDPGNEVK LRLYALYKQA TEGPCNMPKP GMLDFVNKAK WDAWNALGSL
110 120 130 140 150
PKETARQNYV DLVSSLSSSS EAPSQGKRGA DEKARESKDI LVTSEDGITK
160 170 180 190 200
ITFNRPTKKN AISFQMYRDI ILALKNASTD NTVMAVFTGT GDYYCSGNDL
210 220 230 240 250
TNFTSATGGI EEAASNGAVL LRDFVNSFID FPKPLVAVVN GPAVGISVTL
260 270 280 290 300
LGLFDAVFAS DRATFHTPFS QLGQSPEACS SYTFPKMMGS AKAAEMLLFG
310 320 330 340 350
KKLTAREAWA QGLVTEVFPE STFETEVWTR LKTYAKLPPN AMRISKELIR
360 370 380 390
KNEKEKLYAV NAEECTTLQA RWLSEECMNA IMSFVSRKPK L
Length:391
Mass (Da):43,268
Last modified:September 1, 2009 - v2
Checksum:i0B93D3908DF77AF8
GO
Isoform 2 (identifier: Q9WUR2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-33: Missing.

Show »
Length:358
Mass (Da):39,503
Checksum:i2E22207290123AE5
GO

Sequence cautioni

The sequence AAH01983 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35R → K in BAB26315 (PubMed:16141072).Curated1
Sequence conflicti128R → C in AAH01983 (PubMed:15489334).Curated1
Sequence conflicti135R → Q in AAH01983 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0378551 – 33Missing in isoform 2. 1 PublicationAdd BLAST33

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF153613 mRNA Translation: AAD34174.1
AK009478 mRNA Translation: BAB26315.2
BC001983 mRNA Translation: AAH01983.1 Different initiation.
CCDSiCCDS26450.1 [Q9WUR2-2]
CCDS49234.1 [Q9WUR2-1]
RefSeqiNP_001103801.1, NM_001110331.1 [Q9WUR2-1]
NP_035998.2, NM_011868.3 [Q9WUR2-2]
XP_006516736.1, XM_006516673.2 [Q9WUR2-2]
XP_006516737.1, XM_006516674.2 [Q9WUR2-2]
XP_006516738.1, XM_006516675.2 [Q9WUR2-2]
XP_006516739.1, XM_006516676.3 [Q9WUR2-2]
XP_006516740.1, XM_006516677.1 [Q9WUR2-2]
UniGeneiMm.491141

Genome annotation databases

EnsembliENSMUST00000021854; ENSMUSP00000021854; ENSMUSG00000021417 [Q9WUR2-2]
ENSMUST00000171229; ENSMUSP00000131735; ENSMUSG00000021417 [Q9WUR2-1]
ENSMUST00000178421; ENSMUSP00000137411; ENSMUSG00000021417 [Q9WUR2-2]
GeneIDi23986
KEGGimmu:23986
UCSCiuc007qbx.3 mouse [Q9WUR2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiECI2_MOUSE
AccessioniPrimary (citable) accession number: Q9WUR2
Secondary accession number(s): Q99M61, Q9D785
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: September 1, 2009
Last modified: May 23, 2018
This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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