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Protein

Prolactin regulatory element-binding protein

Gene

Preb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor that specifically activates the small GTPase SAR1B. Mediates the recruitement of SAR1B and other COPII coat components to endoplasmic reticulum membranes and is therefore required for the formation of COPII transport vesicles from the ER.1 Publication
Was first identified based on its probable role in the regulation of pituitary gene transcription. Binds to the prolactin gene (PRL) promoter and seems to activate transcription.By similarity

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • DNA binding transcription factor activity Source: MGI
  • GTPase activator activity Source: GO_Central
  • GTPase binding Source: UniProtKB
  • Sar guanyl-nucleotide exchange factor activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processER-Golgi transport, Protein transport, Transcription, Transcription regulation, Transport

Enzyme and pathway databases

ReactomeiR-MMU-204005 COPII (Coat Protein 2) Mediated Vesicle Transport
R-MMU-5694530 Cargo concentration in the ER

Names & Taxonomyi

Protein namesi
Recommended name:
Prolactin regulatory element-binding protein
Alternative name(s):
Mammalian guanine nucleotide exchange factor mSec12
Gene namesi
Name:Preb
Synonyms:Sec12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1355326 Preb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 388CytoplasmicSequence analysisAdd BLAST388
Transmembranei389 – 409HelicalSequence analysisAdd BLAST21
Topological domaini410 – 417LumenalSequence analysis8

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000511551 – 417Prolactin regulatory element-binding proteinAdd BLAST417

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10Nitrated tyrosineCombined sources1

Keywords - PTMi

Nitration

Proteomic databases

EPDiQ9WUQ2
MaxQBiQ9WUQ2
PaxDbiQ9WUQ2
PRIDEiQ9WUQ2

PTM databases

iPTMnetiQ9WUQ2
PhosphoSitePlusiQ9WUQ2
SwissPalmiQ9WUQ2

Expressioni

Developmental stagei

Detected starting at E10.5 in the ectoderm and superficial mesoderm surrounding the caudal part of the tail region. At E12.5 detected in dorsal root ganglia, surface ectoderm surrounding the caudal part of the tail, the inferior wall of the genital tubercle, developing liver, in Rathke pouch and in condensing mesenchyme forming the roof of the skull. At E14.5 detected in the perichondrial region of the craniofacial, axial, and appendicular skeleton. By E16.5 expression is much lower throughout the embryo, and is not detectable by E18.5.1 Publication

Gene expression databases

BgeeiENSMUSG00000045302
CleanExiMM_PREB
ExpressionAtlasiQ9WUQ2 baseline and differential
GenevisibleiQ9WUQ2 MM

Interactioni

Subunit structurei

Interacts with SAR1B (GDP-bound form) (PubMed:11422940). Interacts with CTAGE5; recruits PREB to endoplasmic reticulum exit sites (By similarity).By similarity1 Publication

GO - Molecular functioni

  • GTPase binding Source: UniProtKB
  • Sar guanyl-nucleotide exchange factor activity Source: UniProtKB

Protein-protein interaction databases

BioGridi2061475 interactors.
IntActiQ9WUQ2 2 interactors.
MINTiQ9WUQ2
STRINGi10090.ENSMUSP00000074387

Structurei

3D structure databases

ProteinModelPortaliQ9WUQ2
SMRiQ9WUQ2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati152 – 191WD 1Add BLAST40
Repeati194 – 232WD 2Add BLAST39
Repeati298 – 337WD 3Add BLAST40

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, WD repeat

Phylogenomic databases

eggNOGiKOG0771 Eukaryota
ENOG410XRQK LUCA
GeneTreeiENSGT00390000018031
HOGENOMiHOG000045302
HOVERGENiHBG002027
InParanoidiQ9WUQ2
KOiK14003
OMAiAHSMFVT
OrthoDBiEOG091G06TN
PhylomeDBiQ9WUQ2
TreeFamiTF314383

Family and domain databases

InterProiView protein in InterPro
IPR011047 Quinoprotein_ADH-like_supfam
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
PfamiView protein in Pfam
PF00400 WD40, 1 hit
SMARTiView protein in SMART
SM00320 WD40, 3 hits
SUPFAMiSSF50998 SSF50998, 2 hits
PROSITEiView protein in PROSITE
PS50082 WD_REPEATS_2, 1 hit
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

Q9WUQ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRRRGVELY RAPFPLYALR IDPKTGLLIA AGGGGAAKTG IKNGVHFLQL
60 70 80 90 100
ELINGCLSAS LLHSHDTETR ATMNLALAGD ILAAGQDAQC QLLRFQVHQQ
110 120 130 140 150
KGSKAEKSGS KEQGPRQRKG APPAEKKSGA QVHPEGVELK VKNLEAVQTD
160 170 180 190 200
FSNEPLQKVV CFNHDNTLLA TGGTDGHVRV WKVPSLEKVL EFKAHEGEIG
210 220 230 240 250
DLTLGPDGKL VTVGWDFKAS VWQKDQLVTQ LQWQENGPAS SNTPYRYQAC
260 270 280 290 300
RFGQVPDQLG GLRLFTVQIP HKRLRQPPPC YLTAWDSSTF LPLRTRSCGH
310 320 330 340 350
EVISCLSVSD SGTFLGLGTV TGSVAIYIAF SLQRLYYVKE AHGIVVTDVT
360 370 380 390 400
FLPEKGCGPK LLGPHETALF SVAVDSRCQL HLLPSRRSVP VWLLLLLCVG
410
LIIVTILLLQ TAFPGFL
Length:417
Mass (Da):45,437
Last modified:November 1, 1999 - v1
Checksum:i89F8B24F9A7684FA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti136Missing in AAF07954 (PubMed:10704286).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF150808 mRNA Translation: AAD31722.1
AF193017 mRNA Translation: AAF07954.1
BC017527 mRNA Translation: AAH17527.1
CCDSiCCDS39051.1
RefSeqiNP_057912.2, NM_016703.3
UniGeneiMm.272414

Genome annotation databases

EnsembliENSMUST00000074840; ENSMUSP00000074387; ENSMUSG00000045302
GeneIDi50907
KEGGimmu:50907
UCSCiuc012dui.2 mouse

Similar proteinsi

Entry informationi

Entry nameiPREB_MOUSE
AccessioniPrimary (citable) accession number: Q9WUQ2
Secondary accession number(s): Q9QZ99
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 24, 2001
Last sequence update: November 1, 1999
Last modified: February 28, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome