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Q9WUQ2

- PREB_MOUSE

UniProt

Q9WUQ2 - PREB_MOUSE

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Protein

Prolactin regulatory element-binding protein

Gene

Preb

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Was first identified based on its probable role in the regulation of pituitary gene transcription. Binds to the prolactin gene (PRL) promoter and seems to activate transcription (By similarity). Guanine nucleotide exchange factor that activates SARA2. Required for the formation of COPII transport vesicles from the ER.By similarity1 Publication

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. sequence-specific DNA binding transcription factor activity Source: MGI

GO - Biological processi

  1. protein transport Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
  3. vesicle-mediated transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transcription, Transcription regulation, Transport

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_106572. XBP1(S) activates chaperone genes.
REACT_205304. COPII (Coat Protein 2) Mediated Vesicle Transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Prolactin regulatory element-binding protein
Alternative name(s):
Mammalian guanine nucleotide exchange factor mSec12
Gene namesi
Name:Preb
Synonyms:Sec12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:1355326. Preb.

Subcellular locationi

Nucleus By similarity. Membrane 1 Publication; Single-pass membrane protein 1 Publication. Endoplasmic reticulum 1 Publication

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 417417Prolactin regulatory element-binding proteinPRO_0000051155Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei10 – 101Nitrated tyrosine1 Publication

Keywords - PTMi

Nitration

Proteomic databases

MaxQBiQ9WUQ2.
PaxDbiQ9WUQ2.
PRIDEiQ9WUQ2.

PTM databases

PhosphoSiteiQ9WUQ2.

Expressioni

Developmental stagei

Detected starting at E10.5 in the ectoderm and superficial mesoderm surrounding the caudal part of the tail region. At E12.5 detected in dorsal root ganglia, surface ectoderm surrounding the caudal part of the tail, the inferior wall of the genital tubercle, developing liver, in Rathke pouch and in condensing mesenchyme forming the roof of the skull. At E14.5 detected in the perichondrial region of the craniofacial, axial, and appendicular skeleton. By E16.5 expression is much lower throughout the embryo, and is not detectable by E18.5.1 Publication

Gene expression databases

BgeeiQ9WUQ2.
CleanExiMM_PREB.
ExpressionAtlasiQ9WUQ2. baseline and differential.
GenevestigatoriQ9WUQ2.

Interactioni

Subunit structurei

Binds SARA2.

Protein-protein interaction databases

BioGridi206147. 4 interactions.
IntActiQ9WUQ2. 2 interactions.
MINTiMINT-4108544.

Structurei

3D structure databases

ProteinModelPortaliQ9WUQ2.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 388388CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini410 – 4178LumenalSequence Analysis

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei389 – 40921HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati152 – 19140WD 1Add
BLAST
Repeati194 – 23239WD 2Add
BLAST
Repeati298 – 33740WD 3Add
BLAST

Sequence similaritiesi

Contains 3 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, WD repeat

Phylogenomic databases

eggNOGiNOG309181.
GeneTreeiENSGT00390000018031.
HOGENOMiHOG000045302.
HOVERGENiHBG002027.
InParanoidiQ9WUQ2.
KOiK14003.
OMAiAAGQDAH.
PhylomeDBiQ9WUQ2.
TreeFamiTF314383.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR011047. Quinonprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 2 hits.
[Graphical view]
SMARTiSM00320. WD40. 3 hits.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 2 hits.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WUQ2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGRRRGVELY RAPFPLYALR IDPKTGLLIA AGGGGAAKTG IKNGVHFLQL
60 70 80 90 100
ELINGCLSAS LLHSHDTETR ATMNLALAGD ILAAGQDAQC QLLRFQVHQQ
110 120 130 140 150
KGSKAEKSGS KEQGPRQRKG APPAEKKSGA QVHPEGVELK VKNLEAVQTD
160 170 180 190 200
FSNEPLQKVV CFNHDNTLLA TGGTDGHVRV WKVPSLEKVL EFKAHEGEIG
210 220 230 240 250
DLTLGPDGKL VTVGWDFKAS VWQKDQLVTQ LQWQENGPAS SNTPYRYQAC
260 270 280 290 300
RFGQVPDQLG GLRLFTVQIP HKRLRQPPPC YLTAWDSSTF LPLRTRSCGH
310 320 330 340 350
EVISCLSVSD SGTFLGLGTV TGSVAIYIAF SLQRLYYVKE AHGIVVTDVT
360 370 380 390 400
FLPEKGCGPK LLGPHETALF SVAVDSRCQL HLLPSRRSVP VWLLLLLCVG
410
LIIVTILLLQ TAFPGFL
Length:417
Mass (Da):45,437
Last modified:November 1, 1999 - v1
Checksum:i89F8B24F9A7684FA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti136 – 1361Missing in AAF07954. (PubMed:10704286)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF150808 mRNA. Translation: AAD31722.1.
AF193017 mRNA. Translation: AAF07954.1.
BC017527 mRNA. Translation: AAH17527.1.
CCDSiCCDS39051.1.
RefSeqiNP_057912.2. NM_016703.3.
UniGeneiMm.272414.

Genome annotation databases

EnsembliENSMUST00000074840; ENSMUSP00000074387; ENSMUSG00000045302.
GeneIDi50907.
KEGGimmu:50907.
UCSCiuc012dui.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF150808 mRNA. Translation: AAD31722.1 .
AF193017 mRNA. Translation: AAF07954.1 .
BC017527 mRNA. Translation: AAH17527.1 .
CCDSi CCDS39051.1.
RefSeqi NP_057912.2. NM_016703.3.
UniGenei Mm.272414.

3D structure databases

ProteinModelPortali Q9WUQ2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 206147. 4 interactions.
IntActi Q9WUQ2. 2 interactions.
MINTi MINT-4108544.

PTM databases

PhosphoSitei Q9WUQ2.

Proteomic databases

MaxQBi Q9WUQ2.
PaxDbi Q9WUQ2.
PRIDEi Q9WUQ2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000074840 ; ENSMUSP00000074387 ; ENSMUSG00000045302 .
GeneIDi 50907.
KEGGi mmu:50907.
UCSCi uc012dui.1. mouse.

Organism-specific databases

CTDi 10113.
MGIi MGI:1355326. Preb.

Phylogenomic databases

eggNOGi NOG309181.
GeneTreei ENSGT00390000018031.
HOGENOMi HOG000045302.
HOVERGENi HBG002027.
InParanoidi Q9WUQ2.
KOi K14003.
OMAi AAGQDAH.
PhylomeDBi Q9WUQ2.
TreeFami TF314383.

Enzyme and pathway databases

Reactomei REACT_106572. XBP1(S) activates chaperone genes.
REACT_205304. COPII (Coat Protein 2) Mediated Vesicle Transport.

Miscellaneous databases

ChiTaRSi PREB. mouse.
NextBioi 307881.
PROi Q9WUQ2.
SOURCEi Search...

Gene expression databases

Bgeei Q9WUQ2.
CleanExi MM_PREB.
ExpressionAtlasi Q9WUQ2. baseline and differential.
Genevestigatori Q9WUQ2.

Family and domain databases

Gene3Di 2.130.10.10. 2 hits.
InterProi IPR011047. Quinonprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF00400. WD40. 2 hits.
[Graphical view ]
SMARTi SM00320. WD40. 3 hits.
[Graphical view ]
SUPFAMi SSF50998. SSF50998. 2 hits.
PROSITEi PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The human and mouse homologues of rat prolactin regulatory element binding protein."
    Edgar A.J., Polak J.M.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lung.
  2. "Mapping and developmental expression analysis of the WD-repeat gene Preb."
    Taylor Clelland C.L., Craciun L., Bancroft C., Lufkin T.
    Genomics 63:391-399(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
    Strain: BALB/c.
    Tissue: Brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Salivary gland.
  4. "The mammalian guanine nucleotide exchange factor mSec12 is essential for activation of the Sar1 GTPase directing endoplasmic reticulum export."
    Weissman J.T., Plutner H., Balch W.E.
    Traffic 2:465-475(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. Cited for: NITRATION [LARGE SCALE ANALYSIS] AT TYR-10, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiPREB_MOUSE
AccessioniPrimary (citable) accession number: Q9WUQ2
Secondary accession number(s): Q9QZ99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2001
Last sequence update: November 1, 1999
Last modified: October 29, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3