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Q9WUQ2 (PREB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Prolactin regulatory element-binding protein
Alternative name(s):
Mammalian guanine nucleotide exchange factor mSec12
Gene names
Name:Preb
Synonyms:Sec12
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length417 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Was first identified based on its probable role in the regulation of pituitary gene transcription. Binds to the prolactin gene (PRL) promoter and seems to activate transcription By similarity. Guanine nucleotide exchange factor that activates SARA2. Required for the formation of COPII transport vesicles from the ER. Ref.4

Subunit structure

Binds SARA2.

Subcellular location

Nucleus By similarity. Membrane; Single-pass membrane protein. Endoplasmic reticulum Ref.4.

Developmental stage

Detected starting at E10.5 in the ectoderm and superficial mesoderm surrounding the caudal part of the tail region. At E12.5 detected in dorsal root ganglia, surface ectoderm surrounding the caudal part of the tail, the inferior wall of the genital tubercle, developing liver, in Rathke pouch and in condensing mesenchyme forming the roof of the skull. At E14.5 detected in the perichondrial region of the craniofacial, axial, and appendicular skeleton. By E16.5 expression is much lower throughout the embryo, and is not detectable by E18.5. Ref.2

Sequence similarities

Contains 3 WD repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 417417Prolactin regulatory element-binding protein
PRO_0000051155

Regions

Topological domain1 – 388388Cytoplasmic Potential
Transmembrane389 – 40921Helical; Potential
Topological domain410 – 4178Lumenal Potential
Repeat152 – 19140WD 1
Repeat194 – 23239WD 2
Repeat298 – 33740WD 3

Amino acid modifications

Modified residue101Nitrated tyrosine Ref.5

Experimental info

Sequence conflict1361Missing in AAF07954. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9WUQ2 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 89F8B24F9A7684FA

FASTA41745,437
        10         20         30         40         50         60 
MGRRRGVELY RAPFPLYALR IDPKTGLLIA AGGGGAAKTG IKNGVHFLQL ELINGCLSAS 

        70         80         90        100        110        120 
LLHSHDTETR ATMNLALAGD ILAAGQDAQC QLLRFQVHQQ KGSKAEKSGS KEQGPRQRKG 

       130        140        150        160        170        180 
APPAEKKSGA QVHPEGVELK VKNLEAVQTD FSNEPLQKVV CFNHDNTLLA TGGTDGHVRV 

       190        200        210        220        230        240 
WKVPSLEKVL EFKAHEGEIG DLTLGPDGKL VTVGWDFKAS VWQKDQLVTQ LQWQENGPAS 

       250        260        270        280        290        300 
SNTPYRYQAC RFGQVPDQLG GLRLFTVQIP HKRLRQPPPC YLTAWDSSTF LPLRTRSCGH 

       310        320        330        340        350        360 
EVISCLSVSD SGTFLGLGTV TGSVAIYIAF SLQRLYYVKE AHGIVVTDVT FLPEKGCGPK 

       370        380        390        400        410 
LLGPHETALF SVAVDSRCQL HLLPSRRSVP VWLLLLLCVG LIIVTILLLQ TAFPGFL 

« Hide

References

« Hide 'large scale' references
[1]"The human and mouse homologues of rat prolactin regulatory element binding protein."
Edgar A.J., Polak J.M.
Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Lung.
[2]"Mapping and developmental expression analysis of the WD-repeat gene Preb."
Taylor Clelland C.L., Craciun L., Bancroft C., Lufkin T.
Genomics 63:391-399(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
Strain: BALB/c.
Tissue: Brain.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Salivary gland.
[4]"The mammalian guanine nucleotide exchange factor mSec12 is essential for activation of the Sar1 GTPase directing endoplasmic reticulum export."
Weissman J.T., Plutner H., Balch W.E.
Traffic 2:465-475(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]"Endogenously nitrated proteins in mouse brain: links to neurodegenerative disease."
Sacksteder C.A., Qian W.-J., Knyushko T.V., Wang H., Chin M.H., Lacan G., Melega W.P., Camp D.G. II, Smith R.D., Smith D.J., Squier T.C., Bigelow D.J.
Biochemistry 45:8009-8022(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NITRATION [LARGE SCALE ANALYSIS] AT TYR-10, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF150808 mRNA. Translation: AAD31722.1.
AF193017 mRNA. Translation: AAF07954.1.
BC017527 mRNA. Translation: AAH17527.1.
CCDSCCDS39051.1.
RefSeqNP_057912.2. NM_016703.2.
UniGeneMm.272414.

3D structure databases

ProteinModelPortalQ9WUQ2.
SMRQ9WUQ2. Positions 290-357.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid206147. 4 interactions.
IntActQ9WUQ2. 2 interactions.
MINTMINT-4108544.

PTM databases

PhosphoSiteQ9WUQ2.

Proteomic databases

MaxQBQ9WUQ2.
PaxDbQ9WUQ2.
PRIDEQ9WUQ2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000074840; ENSMUSP00000074387; ENSMUSG00000045302.
GeneID50907.
KEGGmmu:50907.
UCSCuc012dui.1. mouse.

Organism-specific databases

CTD10113.
MGIMGI:1355326. Preb.

Phylogenomic databases

eggNOGNOG309181.
GeneTreeENSGT00390000018031.
HOGENOMHOG000045302.
HOVERGENHBG002027.
InParanoidQ9WUQ2.
KOK14003.
OMAAAGQDAH.
PhylomeDBQ9WUQ2.
TreeFamTF314383.

Gene expression databases

ArrayExpressQ9WUQ2.
BgeeQ9WUQ2.
CleanExMM_PREB.
GenevestigatorQ9WUQ2.

Family and domain databases

Gene3D2.130.10.10. 2 hits.
InterProIPR011047. Quinonprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF00400. WD40. 2 hits.
[Graphical view]
SMARTSM00320. WD40. 3 hits.
[Graphical view]
SUPFAMSSF50998. SSF50998. 2 hits.
PROSITEPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPREB. mouse.
NextBio307881.
PROQ9WUQ2.
SOURCESearch...

Entry information

Entry namePREB_MOUSE
AccessionPrimary (citable) accession number: Q9WUQ2
Secondary accession number(s): Q9QZ99
Entry history
Integrated into UniProtKB/Swiss-Prot: October 24, 2001
Last sequence update: November 1, 1999
Last modified: July 9, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot