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Protein

Ubiquitin carboxyl-terminal hydrolase isozyme L5

Gene

Uchl5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1 (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulationi

Activated by ADRM1. Inhibited by interaction with NFRKB (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei88NucleophileBy similarity1
Active sitei164Proton donorBy similarity1
Sitei179Important for enzyme activityBy similarity1

GO - Molecular functioni

GO - Biological processi

  • DNA recombination Source: UniProtKB-KW
  • DNA repair Source: UniProtKB-KW
  • forebrain morphogenesis Source: BHF-UCL
  • lateral ventricle development Source: BHF-UCL
  • midbrain development Source: BHF-UCL
  • protein deubiquitination Source: UniProtKB
  • regulation of proteasomal protein catabolic process Source: MGI
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
  • ubiquitin-dependent protein catabolic process Source: InterPro

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processDNA damage, DNA recombination, DNA repair, Transcription, Transcription regulation, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-5689603 UCH proteinases

Protein family/group databases

MEROPSiC12.005

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase isozyme L5 (EC:3.4.19.12)
Short name:
UCH-L5
Alternative name(s):
Ubiquitin C-terminal hydrolase UCH37
Ubiquitin thioesterase L5
Gene namesi
Name:Uchl5
Synonyms:Uch37
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi

Organism-specific databases

MGIiMGI:1914848 Uchl5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002110671 – 329Ubiquitin carboxyl-terminal hydrolase isozyme L5Add BLAST329

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei47N6-succinyllysineCombined sources1
Modified residuei158N6-acetyllysineCombined sources1
Modified residuei289N6-succinyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9WUP7
PeptideAtlasiQ9WUP7
PRIDEiQ9WUP7

2D gel databases

REPRODUCTION-2DPAGEiQ9WUP7

PTM databases

iPTMnetiQ9WUP7
PhosphoSitePlusiQ9WUP7
SwissPalmiQ9WUP7

Expressioni

Gene expression databases

BgeeiENSMUSG00000018189
CleanExiMM_UCHL5
ExpressionAtlasiQ9WUP7 baseline and differential
GenevisibleiQ9WUP7 MM

Interactioni

Subunit structurei

Component of the 19S (PA700) regulatory complex of the 26S proteasome. Interacts with ADRM1 and NFRKB. Component of the INO80 complex; specifically part of a complex module associated with N-terminus of INO80 (By similarity).By similarity

Protein-protein interaction databases

BioGridi207842, 6 interactors
IntActiQ9WUP7, 2 interactors
MINTiQ9WUP7
STRINGi10090.ENSMUSP00000018333

Structurei

Secondary structure

1329
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi15 – 24Combined sources10
Beta strandi28 – 34Combined sources7
Helixi41 – 44Combined sources4
Beta strandi48 – 57Combined sources10
Beta strandi65 – 68Combined sources4
Helixi72 – 75Combined sources4
Helixi85 – 87Combined sources3
Helixi88 – 97Combined sources10
Helixi108 – 118Combined sources11
Helixi123 – 131Combined sources9
Helixi134 – 142Combined sources9
Beta strandi163 – 171Combined sources9
Beta strandi174 – 178Combined sources5
Beta strandi182 – 184Combined sources3
Beta strandi186 – 190Combined sources5
Beta strandi193 – 195Combined sources3
Helixi197 – 209Combined sources13
Beta strandi219 – 225Combined sources7
Helixi227 – 244Combined sources18
Helixi256 – 289Combined sources34
Helixi293 – 305Combined sources13
Helixi309 – 318Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4WLQX-ray2.85A1-329[»]
4WLRX-ray2.00A1-329[»]
ProteinModelPortaliQ9WUP7
SMRiQ9WUP7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni313 – 329Interaction with ADRM1By similarityAdd BLAST17

Sequence similaritiesi

Belongs to the peptidase C12 family.Curated

Phylogenomic databases

eggNOGiKOG2778 Eukaryota
ENOG410XP0P LUCA
GeneTreeiENSGT00510000046560
HOGENOMiHOG000203918
HOVERGENiHBG056021
InParanoidiQ9WUP7
KOiK05610
OMAiTIQNACA
OrthoDBiEOG091G0EO2
PhylomeDBiQ9WUP7
TreeFamiTF313976

Family and domain databases

CDDicd02255 Peptidase_C12, 1 hit
Gene3Di3.40.532.10, 1 hit
InterProiView protein in InterPro
IPR001578 Peptidase_C12_UCH
IPR036959 Peptidase_C12_UCH_sf
IPR017390 Ubiquitinyl_hydrolase_UCH37
IPR033837 UCH37
PANTHERiPTHR10589 PTHR10589, 1 hit
PfamiView protein in Pfam
PF01088 Peptidase_C12, 1 hit
PIRSFiPIRSF038120 Ubiquitinyl_hydrolase_UCH37, 1 hit
PRINTSiPR00707 UBCTHYDRLASE

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9WUP7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ESFEKLKPVH
60 70 80 90 100
GLIFLFKWQP GEEPAGSVVQ DSRLETIFFA KQVINNACAT QAIVSVLLNC
110 120 130 140 150
THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE
160 170 180 190 200
FDTKTPAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWITAV
210 220 230 240 250
RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEEPMDT
260 270 280 290 300
DQGSTVLSAI QSEVARNQML IEEEVQKLKR YKIENIRRKH NYLPFIMELL
310 320
KTLAEHQQLI PLVEKAKEKQ NAKKAQETK
Length:329
Mass (Da):37,617
Last modified:September 26, 2001 - v2
Checksum:i1EAB5223FB707917
GO
Isoform 2 (identifier: Q9WUP7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     246-246: Missing.

Show »
Length:328
Mass (Da):37,488
Checksum:i55483B16ADC1E720
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20E → K in AAD31534 (Ref. 1) Curated1
Sequence conflicti24G → R in AAD31534 (Ref. 1) Curated1
Sequence conflicti104D → E in BAB25312 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005255246Missing in isoform 2. 3 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF148447 mRNA Translation: AAD31534.1
AF175903 mRNA Translation: AAD50311.1
AK017925 mRNA Translation: BAB31005.1
AK011117 mRNA Translation: BAB27412.1
AK007860 mRNA Translation: BAB25312.1
BC006891 mRNA Translation: AAH06891.1
CCDSiCCDS15346.1 [Q9WUP7-1]
CCDS78699.1 [Q9WUP7-2]
RefSeqiNP_001153338.1, NM_001159866.1 [Q9WUP7-2]
NP_062508.2, NM_019562.2 [Q9WUP7-1]
UniGeneiMm.261004

Genome annotation databases

EnsembliENSMUST00000018333; ENSMUSP00000018333; ENSMUSG00000018189 [Q9WUP7-2]
ENSMUST00000189936; ENSMUSP00000140106; ENSMUSG00000018189 [Q9WUP7-1]
GeneIDi56207
KEGGimmu:56207
UCSCiuc007cxe.2 mouse [Q9WUP7-1]
uc007cxf.2 mouse [Q9WUP7-2]

Keywords - Coding sequence diversityi

Alternative splicing

Entry informationi

Entry nameiUCHL5_MOUSE
AccessioniPrimary (citable) accession number: Q9WUP7
Secondary accession number(s): Q9CVJ4, Q9CXZ3, Q9R107
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: September 26, 2001
Last modified: May 23, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

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