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Q9WUP7

- UCHL5_MOUSE

UniProt

Q9WUP7 - UCHL5_MOUSE

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Protein

Ubiquitin carboxyl-terminal hydrolase isozyme L5

Gene
Uchl5, Uch37
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1 By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulationi

Activated by ADRM1. Inhibited by interaction with NFRKB By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei88 – 881Nucleophile By similarity
Active sitei164 – 1641Proton donor By similarity
Sitei179 – 1791Important for enzyme activity By similarity

GO - Molecular functioni

  1. endopeptidase inhibitor activity Source: Ensembl
  2. omega peptidase activity Source: InterPro
  3. ubiquitin-specific protease activity Source: UniProtKB
  4. ubiquitin thiolesterase activity Source: Reactome

GO - Biological processi

  1. DNA recombination Source: UniProtKB-KW
  2. DNA repair Source: UniProtKB-KW
  3. forebrain morphogenesis Source: BHF-UCL
  4. lateral ventricle development Source: BHF-UCL
  5. midbrain development Source: BHF-UCL
  6. protein deubiquitination Source: UniProtKB
  7. regulation of proteasomal protein catabolic process Source: Ensembl
  8. regulation of transcription, DNA-templated Source: UniProtKB-KW
  9. transcription, DNA-templated Source: UniProtKB-KW
  10. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, Transcription, Transcription regulation, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiREACT_215733. Downregulation of TGF-beta receptor signaling.

Protein family/group databases

MEROPSiC12.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase isozyme L5 (EC:3.4.19.12)
Short name:
UCH-L5
Alternative name(s):
Ubiquitin C-terminal hydrolase UCH37
Ubiquitin thioesterase L5
Gene namesi
Name:Uchl5
Synonyms:Uch37
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1914848. Uchl5.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity
Note: Associates with the proteasome 19S subunit in the cytoplasm. Associates with the INO80 complex in the nucleus By similarity.

GO - Cellular componenti

  1. cytosol Source: UniProtKB
  2. Ino80 complex Source: Ensembl
  3. nucleus Source: UniProtKB
  4. proteasome complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 329329Ubiquitin carboxyl-terminal hydrolase isozyme L5PRO_0000211067Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei47 – 471N6-succinyllysine1 Publication
Modified residuei158 – 1581N6-acetyllysine1 Publication
Modified residuei289 – 2891N6-succinyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ9WUP7.
PaxDbiQ9WUP7.
PRIDEiQ9WUP7.

2D gel databases

REPRODUCTION-2DPAGEQ9WUP7.

PTM databases

PhosphoSiteiQ9WUP7.

Expressioni

Gene expression databases

BgeeiQ9WUP7.
CleanExiMM_UCHL5.
GenevestigatoriQ9WUP7.

Interactioni

Subunit structurei

Component of the 19S (PA700) regulatory complex of the 26S proteasome. Interacts with ADRM1 and NFRKB. Component of the INO80 complex; specifically part of a complex module associated with N-terminus of INO80 By similarity.

Protein-protein interaction databases

BioGridi207842. 1 interaction.
IntActiQ9WUP7. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9WUP7.
SMRiQ9WUP7. Positions 7-312.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni313 – 32917Interaction with ADRM1 By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C12 family.

Phylogenomic databases

eggNOGiNOG321645.
GeneTreeiENSGT00510000046560.
HOGENOMiHOG000203918.
HOVERGENiHBG056021.
InParanoidiQ9WUP7.
KOiK05610.
OMAiDEDAFHF.
OrthoDBiEOG7M3J11.
PhylomeDBiQ9WUP7.
TreeFamiTF313976.

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiIPR001578. Peptidase_C12_UCH.
IPR017390. Ubiquitinyl_hydrolase_UCH37.
[Graphical view]
PANTHERiPTHR10589. PTHR10589. 1 hit.
PfamiPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PIRSFiPIRSF038120. Ubiquitinyl_hydrolase_UCH37. 1 hit.
PRINTSiPR00707. UBCTHYDRLASE.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9WUP7-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSSNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ESFEKLKPVH    50
GLIFLFKWQP GEEPAGSVVQ DSRLETIFFA KQVINNACAT QAIVSVLLNC 100
THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE 150
FDTKTPAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWITAV 200
RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEEPMDT 250
DQGSTVLSAI QSEVARNQML IEEEVQKLKR YKIENIRRKH NYLPFIMELL 300
KTLAEHQQLI PLVEKAKEKQ NAKKAQETK 329
Length:329
Mass (Da):37,617
Last modified:September 26, 2001 - v2
Checksum:i1EAB5223FB707917
GO
Isoform 2 (identifier: Q9WUP7-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     246-246: Missing.

Show »
Length:328
Mass (Da):37,488
Checksum:i55483B16ADC1E720
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei246 – 2461Missing in isoform 2. VSP_005255

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti20 – 201E → K in AAD31534. 1 Publication
Sequence conflicti24 – 241G → R in AAD31534. 1 Publication
Sequence conflicti104 – 1041D → E in BAB25312. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF148447 mRNA. Translation: AAD31534.1.
AF175903 mRNA. Translation: AAD50311.1.
AK017925 mRNA. Translation: BAB31005.1.
AK011117 mRNA. Translation: BAB27412.1.
AK007860 mRNA. Translation: BAB25312.1.
BC006891 mRNA. Translation: AAH06891.1.
CCDSiCCDS15346.1. [Q9WUP7-1]
RefSeqiNP_001153338.1. NM_001159866.1. [Q9WUP7-2]
NP_062508.2. NM_019562.2. [Q9WUP7-1]
UniGeneiMm.261004.

Genome annotation databases

EnsembliENSMUST00000018333; ENSMUSP00000018333; ENSMUSG00000018189. [Q9WUP7-1]
GeneIDi56207.
KEGGimmu:56207.
UCSCiuc007cxe.2. mouse. [Q9WUP7-1]
uc007cxf.2. mouse. [Q9WUP7-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF148447 mRNA. Translation: AAD31534.1 .
AF175903 mRNA. Translation: AAD50311.1 .
AK017925 mRNA. Translation: BAB31005.1 .
AK011117 mRNA. Translation: BAB27412.1 .
AK007860 mRNA. Translation: BAB25312.1 .
BC006891 mRNA. Translation: AAH06891.1 .
CCDSi CCDS15346.1. [Q9WUP7-1 ]
RefSeqi NP_001153338.1. NM_001159866.1. [Q9WUP7-2 ]
NP_062508.2. NM_019562.2. [Q9WUP7-1 ]
UniGenei Mm.261004.

3D structure databases

ProteinModelPortali Q9WUP7.
SMRi Q9WUP7. Positions 7-312.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 207842. 1 interaction.
IntActi Q9WUP7. 1 interaction.

Protein family/group databases

MEROPSi C12.005.

PTM databases

PhosphoSitei Q9WUP7.

2D gel databases

REPRODUCTION-2DPAGE Q9WUP7.

Proteomic databases

MaxQBi Q9WUP7.
PaxDbi Q9WUP7.
PRIDEi Q9WUP7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000018333 ; ENSMUSP00000018333 ; ENSMUSG00000018189 . [Q9WUP7-1 ]
GeneIDi 56207.
KEGGi mmu:56207.
UCSCi uc007cxe.2. mouse. [Q9WUP7-1 ]
uc007cxf.2. mouse. [Q9WUP7-2 ]

Organism-specific databases

CTDi 51377.
MGIi MGI:1914848. Uchl5.

Phylogenomic databases

eggNOGi NOG321645.
GeneTreei ENSGT00510000046560.
HOGENOMi HOG000203918.
HOVERGENi HBG056021.
InParanoidi Q9WUP7.
KOi K05610.
OMAi DEDAFHF.
OrthoDBi EOG7M3J11.
PhylomeDBi Q9WUP7.
TreeFami TF313976.

Enzyme and pathway databases

Reactomei REACT_215733. Downregulation of TGF-beta receptor signaling.

Miscellaneous databases

ChiTaRSi UCHL5. mouse.
NextBioi 312039.
PROi Q9WUP7.
SOURCEi Search...

Gene expression databases

Bgeei Q9WUP7.
CleanExi MM_UCHL5.
Genevestigatori Q9WUP7.

Family and domain databases

Gene3Di 3.40.532.10. 1 hit.
InterProi IPR001578. Peptidase_C12_UCH.
IPR017390. Ubiquitinyl_hydrolase_UCH37.
[Graphical view ]
PANTHERi PTHR10589. PTHR10589. 1 hit.
Pfami PF01088. Peptidase_C12. 1 hit.
[Graphical view ]
PIRSFi PIRSF038120. Ubiquitinyl_hydrolase_UCH37. 1 hit.
PRINTSi PR00707. UBCTHYDRLASE.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Xu W., DeMartino G.N., Slaughter C.A., Cohen R.E.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Identification of a novel mouse ubiquitin C-terminal hydrolase: cloning, expression and functional characterization."
    Li T.W., Sun B., Mustafa F.B., Lee M.K., Teo T.S.
    Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Embryonic liver, Pancreas and Thymus.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: Czech II.
    Tissue: Mammary gland.
  5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-158, SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-47 AND LYS-289, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiUCHL5_MOUSE
AccessioniPrimary (citable) accession number: Q9WUP7
Secondary accession number(s): Q9CVJ4, Q9CXZ3, Q9R107
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: September 26, 2001
Last modified: September 3, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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