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Q9WUP7

- UCHL5_MOUSE

UniProt

Q9WUP7 - UCHL5_MOUSE

Protein

Ubiquitin carboxyl-terminal hydrolase isozyme L5

Gene

Uchl5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 2 (26 Sep 2001)
      Previous versions | rss
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    Functioni

    Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1 By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Enzyme regulationi

    Activated by ADRM1. Inhibited by interaction with NFRKB By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei88 – 881NucleophileBy similarity
    Active sitei164 – 1641Proton donorBy similarity
    Sitei179 – 1791Important for enzyme activityBy similarity

    GO - Molecular functioni

    1. endopeptidase inhibitor activity Source: Ensembl
    2. omega peptidase activity Source: InterPro
    3. ubiquitin-specific protease activity Source: UniProtKB
    4. ubiquitin thiolesterase activity Source: Reactome

    GO - Biological processi

    1. DNA recombination Source: UniProtKB-KW
    2. DNA repair Source: UniProtKB-KW
    3. forebrain morphogenesis Source: BHF-UCL
    4. lateral ventricle development Source: BHF-UCL
    5. midbrain development Source: BHF-UCL
    6. protein deubiquitination Source: UniProtKB
    7. regulation of proteasomal protein catabolic process Source: Ensembl
    8. regulation of transcription, DNA-templated Source: UniProtKB-KW
    9. transcription, DNA-templated Source: UniProtKB-KW
    10. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair, Transcription, Transcription regulation, Ubl conjugation pathway

    Enzyme and pathway databases

    ReactomeiREACT_215733. Downregulation of TGF-beta receptor signaling.

    Protein family/group databases

    MEROPSiC12.005.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase isozyme L5 (EC:3.4.19.12)
    Short name:
    UCH-L5
    Alternative name(s):
    Ubiquitin C-terminal hydrolase UCH37
    Ubiquitin thioesterase L5
    Gene namesi
    Name:Uchl5
    Synonyms:Uch37
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:1914848. Uchl5.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity
    Note: Associates with the proteasome 19S subunit in the cytoplasm. Associates with the INO80 complex in the nucleus By similarity.By similarity

    GO - Cellular componenti

    1. cytosol Source: UniProtKB
    2. Ino80 complex Source: Ensembl
    3. nucleus Source: UniProtKB
    4. proteasome complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 329329Ubiquitin carboxyl-terminal hydrolase isozyme L5PRO_0000211067Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei47 – 471N6-succinyllysine1 Publication
    Modified residuei158 – 1581N6-acetyllysine1 Publication
    Modified residuei289 – 2891N6-succinyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ9WUP7.
    PaxDbiQ9WUP7.
    PRIDEiQ9WUP7.

    2D gel databases

    REPRODUCTION-2DPAGEQ9WUP7.

    PTM databases

    PhosphoSiteiQ9WUP7.

    Expressioni

    Gene expression databases

    BgeeiQ9WUP7.
    CleanExiMM_UCHL5.
    GenevestigatoriQ9WUP7.

    Interactioni

    Subunit structurei

    Component of the 19S (PA700) regulatory complex of the 26S proteasome. Interacts with ADRM1 and NFRKB. Component of the INO80 complex; specifically part of a complex module associated with N-terminus of INO80 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi207842. 1 interaction.
    IntActiQ9WUP7. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9WUP7.
    SMRiQ9WUP7. Positions 7-312.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni313 – 32917Interaction with ADRM1By similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C12 family.Curated

    Phylogenomic databases

    eggNOGiNOG321645.
    GeneTreeiENSGT00510000046560.
    HOGENOMiHOG000203918.
    HOVERGENiHBG056021.
    InParanoidiQ9WUP7.
    KOiK05610.
    OMAiDEDAFHF.
    OrthoDBiEOG7M3J11.
    PhylomeDBiQ9WUP7.
    TreeFamiTF313976.

    Family and domain databases

    Gene3Di3.40.532.10. 1 hit.
    InterProiIPR001578. Peptidase_C12_UCH.
    IPR017390. Ubiquitinyl_hydrolase_UCH37.
    [Graphical view]
    PANTHERiPTHR10589. PTHR10589. 1 hit.
    PfamiPF01088. Peptidase_C12. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038120. Ubiquitinyl_hydrolase_UCH37. 1 hit.
    PRINTSiPR00707. UBCTHYDRLASE.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9WUP7-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSSNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ESFEKLKPVH    50
    GLIFLFKWQP GEEPAGSVVQ DSRLETIFFA KQVINNACAT QAIVSVLLNC 100
    THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE 150
    FDTKTPAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWITAV 200
    RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEEPMDT 250
    DQGSTVLSAI QSEVARNQML IEEEVQKLKR YKIENIRRKH NYLPFIMELL 300
    KTLAEHQQLI PLVEKAKEKQ NAKKAQETK 329
    Length:329
    Mass (Da):37,617
    Last modified:September 26, 2001 - v2
    Checksum:i1EAB5223FB707917
    GO
    Isoform 2 (identifier: Q9WUP7-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         246-246: Missing.

    Show »
    Length:328
    Mass (Da):37,488
    Checksum:i55483B16ADC1E720
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti20 – 201E → K in AAD31534. 1 PublicationCurated
    Sequence conflicti24 – 241G → R in AAD31534. 1 PublicationCurated
    Sequence conflicti104 – 1041D → E in BAB25312. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei246 – 2461Missing in isoform 2. 3 PublicationsVSP_005255

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF148447 mRNA. Translation: AAD31534.1.
    AF175903 mRNA. Translation: AAD50311.1.
    AK017925 mRNA. Translation: BAB31005.1.
    AK011117 mRNA. Translation: BAB27412.1.
    AK007860 mRNA. Translation: BAB25312.1.
    BC006891 mRNA. Translation: AAH06891.1.
    CCDSiCCDS15346.1. [Q9WUP7-1]
    RefSeqiNP_001153338.1. NM_001159866.1. [Q9WUP7-2]
    NP_062508.2. NM_019562.2. [Q9WUP7-1]
    UniGeneiMm.261004.

    Genome annotation databases

    EnsembliENSMUST00000018333; ENSMUSP00000018333; ENSMUSG00000018189. [Q9WUP7-1]
    GeneIDi56207.
    KEGGimmu:56207.
    UCSCiuc007cxe.2. mouse. [Q9WUP7-1]
    uc007cxf.2. mouse. [Q9WUP7-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF148447 mRNA. Translation: AAD31534.1 .
    AF175903 mRNA. Translation: AAD50311.1 .
    AK017925 mRNA. Translation: BAB31005.1 .
    AK011117 mRNA. Translation: BAB27412.1 .
    AK007860 mRNA. Translation: BAB25312.1 .
    BC006891 mRNA. Translation: AAH06891.1 .
    CCDSi CCDS15346.1. [Q9WUP7-1 ]
    RefSeqi NP_001153338.1. NM_001159866.1. [Q9WUP7-2 ]
    NP_062508.2. NM_019562.2. [Q9WUP7-1 ]
    UniGenei Mm.261004.

    3D structure databases

    ProteinModelPortali Q9WUP7.
    SMRi Q9WUP7. Positions 7-312.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 207842. 1 interaction.
    IntActi Q9WUP7. 1 interaction.

    Protein family/group databases

    MEROPSi C12.005.

    PTM databases

    PhosphoSitei Q9WUP7.

    2D gel databases

    REPRODUCTION-2DPAGE Q9WUP7.

    Proteomic databases

    MaxQBi Q9WUP7.
    PaxDbi Q9WUP7.
    PRIDEi Q9WUP7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000018333 ; ENSMUSP00000018333 ; ENSMUSG00000018189 . [Q9WUP7-1 ]
    GeneIDi 56207.
    KEGGi mmu:56207.
    UCSCi uc007cxe.2. mouse. [Q9WUP7-1 ]
    uc007cxf.2. mouse. [Q9WUP7-2 ]

    Organism-specific databases

    CTDi 51377.
    MGIi MGI:1914848. Uchl5.

    Phylogenomic databases

    eggNOGi NOG321645.
    GeneTreei ENSGT00510000046560.
    HOGENOMi HOG000203918.
    HOVERGENi HBG056021.
    InParanoidi Q9WUP7.
    KOi K05610.
    OMAi DEDAFHF.
    OrthoDBi EOG7M3J11.
    PhylomeDBi Q9WUP7.
    TreeFami TF313976.

    Enzyme and pathway databases

    Reactomei REACT_215733. Downregulation of TGF-beta receptor signaling.

    Miscellaneous databases

    ChiTaRSi UCHL5. mouse.
    NextBioi 312039.
    PROi Q9WUP7.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9WUP7.
    CleanExi MM_UCHL5.
    Genevestigatori Q9WUP7.

    Family and domain databases

    Gene3Di 3.40.532.10. 1 hit.
    InterProi IPR001578. Peptidase_C12_UCH.
    IPR017390. Ubiquitinyl_hydrolase_UCH37.
    [Graphical view ]
    PANTHERi PTHR10589. PTHR10589. 1 hit.
    Pfami PF01088. Peptidase_C12. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038120. Ubiquitinyl_hydrolase_UCH37. 1 hit.
    PRINTSi PR00707. UBCTHYDRLASE.
    ProtoNeti Search...

    Publicationsi

    1. Xu W., DeMartino G.N., Slaughter C.A., Cohen R.E.
      Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Identification of a novel mouse ubiquitin C-terminal hydrolase: cloning, expression and functional characterization."
      Li T.W., Sun B., Mustafa F.B., Lee M.K., Teo T.S.
      Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J.
      Tissue: Embryonic liver, Pancreas and Thymus.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: Czech II.
      Tissue: Mammary gland.
    5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-158, SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-47 AND LYS-289, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiUCHL5_MOUSE
    AccessioniPrimary (citable) accession number: Q9WUP7
    Secondary accession number(s): Q9CVJ4, Q9CXZ3, Q9R107
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: September 26, 2001
    Last modified: October 1, 2014
    This is version 117 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3