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Protein

Ubiquitin carboxyl-terminal hydrolase isozyme L5

Gene

Uchl5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1 (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulationi

Activated by ADRM1. Inhibited by interaction with NFRKB (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei88 – 881NucleophileBy similarity
Active sitei164 – 1641Proton donorBy similarity
Sitei179 – 1791Important for enzyme activityBy similarity

GO - Molecular functioni

  1. endopeptidase inhibitor activity Source: MGI
  2. omega peptidase activity Source: InterPro
  3. poly(A) RNA binding Source: MGI
  4. proteasome binding Source: MGI
  5. ubiquitin-specific protease activity Source: UniProtKB
  6. ubiquitin thiolesterase activity Source: Reactome

GO - Biological processi

  1. DNA recombination Source: UniProtKB-KW
  2. DNA repair Source: UniProtKB-KW
  3. forebrain morphogenesis Source: BHF-UCL
  4. lateral ventricle development Source: BHF-UCL
  5. midbrain development Source: BHF-UCL
  6. negative regulation of endopeptidase activity Source: MGI
  7. protein deubiquitination Source: UniProtKB
  8. regulation of proteasomal protein catabolic process Source: MGI
  9. regulation of transcription, DNA-templated Source: UniProtKB-KW
  10. transcription, DNA-templated Source: UniProtKB-KW
  11. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, Transcription, Transcription regulation, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiREACT_215733. Downregulation of TGF-beta receptor signaling.

Protein family/group databases

MEROPSiC12.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase isozyme L5 (EC:3.4.19.12)
Short name:
UCH-L5
Alternative name(s):
Ubiquitin C-terminal hydrolase UCH37
Ubiquitin thioesterase L5
Gene namesi
Name:Uchl5
Synonyms:Uch37
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1914848. Uchl5.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity
Note: Associates with the proteasome 19S subunit in the cytoplasm. Associates with the INO80 complex in the nucleus (By similarity).By similarity

GO - Cellular componenti

  1. cytosol Source: UniProtKB
  2. Ino80 complex Source: MGI
  3. nucleus Source: UniProtKB
  4. proteasome complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 329329Ubiquitin carboxyl-terminal hydrolase isozyme L5PRO_0000211067Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei47 – 471N6-succinyllysine1 Publication
Modified residuei158 – 1581N6-acetyllysine1 Publication
Modified residuei289 – 2891N6-succinyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ9WUP7.
PaxDbiQ9WUP7.
PRIDEiQ9WUP7.

2D gel databases

REPRODUCTION-2DPAGEQ9WUP7.

PTM databases

PhosphoSiteiQ9WUP7.

Expressioni

Gene expression databases

BgeeiQ9WUP7.
CleanExiMM_UCHL5.
GenevestigatoriQ9WUP7.

Interactioni

Subunit structurei

Component of the 19S (PA700) regulatory complex of the 26S proteasome. Interacts with ADRM1 and NFRKB. Component of the INO80 complex; specifically part of a complex module associated with N-terminus of INO80 (By similarity).By similarity

Protein-protein interaction databases

BioGridi207842. 2 interactions.
IntActiQ9WUP7. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9WUP7.
SMRiQ9WUP7. Positions 7-312.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni313 – 32917Interaction with ADRM1By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C12 family.Curated

Phylogenomic databases

eggNOGiNOG321645.
GeneTreeiENSGT00510000046560.
HOGENOMiHOG000203918.
HOVERGENiHBG056021.
InParanoidiQ9WUP7.
KOiK05610.
OMAiDEDAFHF.
OrthoDBiEOG7M3J11.
PhylomeDBiQ9WUP7.
TreeFamiTF313976.

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiIPR001578. Peptidase_C12_UCH.
IPR017390. Ubiquitinyl_hydrolase_UCH37.
[Graphical view]
PANTHERiPTHR10589. PTHR10589. 1 hit.
PfamiPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PIRSFiPIRSF038120. Ubiquitinyl_hydrolase_UCH37. 1 hit.
PRINTSiPR00707. UBCTHYDRLASE.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9WUP7-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ESFEKLKPVH
60 70 80 90 100
GLIFLFKWQP GEEPAGSVVQ DSRLETIFFA KQVINNACAT QAIVSVLLNC
110 120 130 140 150
THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE
160 170 180 190 200
FDTKTPAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWITAV
210 220 230 240 250
RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEEPMDT
260 270 280 290 300
DQGSTVLSAI QSEVARNQML IEEEVQKLKR YKIENIRRKH NYLPFIMELL
310 320
KTLAEHQQLI PLVEKAKEKQ NAKKAQETK
Length:329
Mass (Da):37,617
Last modified:September 26, 2001 - v2
Checksum:i1EAB5223FB707917
GO
Isoform 2 (identifier: Q9WUP7-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     246-246: Missing.

Show »
Length:328
Mass (Da):37,488
Checksum:i55483B16ADC1E720
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti20 – 201E → K in AAD31534. 1 PublicationCurated
Sequence conflicti24 – 241G → R in AAD31534. 1 PublicationCurated
Sequence conflicti104 – 1041D → E in BAB25312. 1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei246 – 2461Missing in isoform 2. 3 PublicationsVSP_005255

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF148447 mRNA. Translation: AAD31534.1.
AF175903 mRNA. Translation: AAD50311.1.
AK017925 mRNA. Translation: BAB31005.1.
AK011117 mRNA. Translation: BAB27412.1.
AK007860 mRNA. Translation: BAB25312.1.
BC006891 mRNA. Translation: AAH06891.1.
CCDSiCCDS15346.1. [Q9WUP7-1]
RefSeqiNP_001153338.1. NM_001159866.1. [Q9WUP7-2]
NP_062508.2. NM_019562.2. [Q9WUP7-1]
UniGeneiMm.261004.

Genome annotation databases

EnsembliENSMUST00000018333; ENSMUSP00000018333; ENSMUSG00000018189. [Q9WUP7-2]
ENSMUST00000189936; ENSMUSP00000140106; ENSMUSG00000018189. [Q9WUP7-1]
GeneIDi56207.
KEGGimmu:56207.
UCSCiuc007cxe.2. mouse. [Q9WUP7-1]
uc007cxf.2. mouse. [Q9WUP7-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF148447 mRNA. Translation: AAD31534.1.
AF175903 mRNA. Translation: AAD50311.1.
AK017925 mRNA. Translation: BAB31005.1.
AK011117 mRNA. Translation: BAB27412.1.
AK007860 mRNA. Translation: BAB25312.1.
BC006891 mRNA. Translation: AAH06891.1.
CCDSiCCDS15346.1. [Q9WUP7-1]
RefSeqiNP_001153338.1. NM_001159866.1. [Q9WUP7-2]
NP_062508.2. NM_019562.2. [Q9WUP7-1]
UniGeneiMm.261004.

3D structure databases

ProteinModelPortaliQ9WUP7.
SMRiQ9WUP7. Positions 7-312.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207842. 2 interactions.
IntActiQ9WUP7. 1 interaction.

Protein family/group databases

MEROPSiC12.005.

PTM databases

PhosphoSiteiQ9WUP7.

2D gel databases

REPRODUCTION-2DPAGEQ9WUP7.

Proteomic databases

MaxQBiQ9WUP7.
PaxDbiQ9WUP7.
PRIDEiQ9WUP7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018333; ENSMUSP00000018333; ENSMUSG00000018189. [Q9WUP7-2]
ENSMUST00000189936; ENSMUSP00000140106; ENSMUSG00000018189. [Q9WUP7-1]
GeneIDi56207.
KEGGimmu:56207.
UCSCiuc007cxe.2. mouse. [Q9WUP7-1]
uc007cxf.2. mouse. [Q9WUP7-2]

Organism-specific databases

CTDi51377.
MGIiMGI:1914848. Uchl5.

Phylogenomic databases

eggNOGiNOG321645.
GeneTreeiENSGT00510000046560.
HOGENOMiHOG000203918.
HOVERGENiHBG056021.
InParanoidiQ9WUP7.
KOiK05610.
OMAiDEDAFHF.
OrthoDBiEOG7M3J11.
PhylomeDBiQ9WUP7.
TreeFamiTF313976.

Enzyme and pathway databases

ReactomeiREACT_215733. Downregulation of TGF-beta receptor signaling.

Miscellaneous databases

ChiTaRSiUchl5. mouse.
NextBioi312039.
PROiQ9WUP7.
SOURCEiSearch...

Gene expression databases

BgeeiQ9WUP7.
CleanExiMM_UCHL5.
GenevestigatoriQ9WUP7.

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiIPR001578. Peptidase_C12_UCH.
IPR017390. Ubiquitinyl_hydrolase_UCH37.
[Graphical view]
PANTHERiPTHR10589. PTHR10589. 1 hit.
PfamiPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PIRSFiPIRSF038120. Ubiquitinyl_hydrolase_UCH37. 1 hit.
PRINTSiPR00707. UBCTHYDRLASE.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Xu W., DeMartino G.N., Slaughter C.A., Cohen R.E.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Identification of a novel mouse ubiquitin C-terminal hydrolase: cloning, expression and functional characterization."
    Li T.W., Sun B., Mustafa F.B., Lee M.K., Teo T.S.
    Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Embryonic liver, Pancreas and Thymus.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: Czech II.
    Tissue: Mammary gland.
  5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-158, SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-47 AND LYS-289, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiUCHL5_MOUSE
AccessioniPrimary (citable) accession number: Q9WUP7
Secondary accession number(s): Q9CVJ4, Q9CXZ3, Q9R107
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: September 26, 2001
Last modified: February 4, 2015
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.