ID   S5A3_MOUSE              Reviewed;         330 AA.
AC   Q9WUP4; Q8BGE3;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   16-JUN-2009, entry version 41.
DE   RecName: Full=3-oxo-5-alpha-steroid 4-dehydrogenase 3;
DE            EC=1.3.99.5;
DE   AltName: Full=Steroid 5-alpha-reductase 3;
DE   AltName: Full=SR type 3;
DE   AltName: Full=Steroid 5-alpha-reductase 2-like;
GN   Name=Srd5a3; Synonyms=Srd5a2l;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=129/Sv;
RX   MEDLINE=20568483; PubMed=11116088; DOI=10.1101/gr.10.12.1928;
RA   Wilsbacher L.D., Sangoram A.M., Antoch M.P., Takahashi J.S.;
RT   "The mouse Clock locus: sequence and comparative analysis of 204 kb
RT   from mouse chromosome 5.";
RL   Genome Res. 10:1928-1940(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Cerebellum, Thymus, and Urinary bladder;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M.,
RA   Davis M.J., Wilming L.G., Aidinis V., Allen J.E.,
RA   Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L.,
RA   Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M.,
RA   Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R.,
RA   Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G.,
RA   di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G.,
RA   Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M.,
RA   Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N.,
RA   Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T.,
RA   Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H.,
RA   Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K.,
RA   Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J.,
RA   Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L.,
RA   Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K.,
RA   Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P.,
RA   Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O.,
RA   Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G.,
RA   Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M.,
RA   Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B.,
RA   Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K.,
RA   Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A.,
RA   Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K.,
RA   Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C.,
RA   Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J.,
RA   Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y.,
RA   Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T.,
RA   Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N.,
RA   Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N.,
RA   Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S.,
RA   Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J.,
RA   Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Converts testosterone (T) into 5-alpha-
CC       dihydrotestosterone (DHT) (By similarity).
CC   -!- CATALYTIC ACTIVITY: A 3-oxo-5-alpha-steroid + acceptor = a 3-oxo-
CC       Delta(4)-steroid + reduced acceptor.
CC   -!- SUBCELLULAR LOCATION: Microsome membrane; Multi-pass membrane
CC       protein (By similarity). Endoplasmic reticulum membrane; Multi-
CC       pass membrane protein (Potential).
CC   -!- SIMILARITY: Belongs to the steroid 5-alpha reductase family.
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DR   EMBL; AF146793; AAD30567.1; -; Genomic_DNA.
DR   EMBL; AK040198; BAC30537.1; -; mRNA.
DR   EMBL; AK075635; BAC35871.1; -; mRNA.
DR   EMBL; AK139290; BAE23944.1; -; mRNA.
DR   EMBL; AK162527; BAE36955.1; -; mRNA.
DR   EMBL; BC145647; AAI45648.1; -; mRNA.
DR   IPI; IPI00885969; -.
DR   RefSeq; NP_065636.2; -.
DR   RefSeq; XP_001471752.1; -.
DR   UniGene; Mm.289446; -.
DR   Ensembl; ENSMUSG00000029233; Mus musculus.
DR   GeneID; 100044230; -.
DR   GeneID; 57357; -.
DR   KEGG; mmu:100044230; -.
DR   KEGG; mmu:57357; -.
DR   MGI; MGI:1930252; Srd5a3.
DR   HOVERGEN; Q9WUP4; -.
DR   BRENDA; 1.3.99.5; 244.
DR   NextBio; 457733; -.
DR   ArrayExpress; Q9WUP4; -.
DR   Bgee; Q9WUP4; -.
DR   CleanEx; MM_SRD5A3; -.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005792; C:microsome; IEA:UniProtKB-SubCell.
DR   GO; GO:0003865; F:3-oxo-5-alpha-steroid 4-dehydrogenase activity; IEA:EC.
DR   GO; GO:0006629; P:lipid metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001104; 3-oxo-5_a-steroid_4-DH_C.
DR   Pfam; PF02544; Steroid_dh; 1.
DR   PROSITE; PS50244; S5A_REDUCTASE; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Membrane; Microsome; Oxidoreductase;
KW   Transmembrane.
FT   CHAIN         1    330       3-oxo-5-alpha-steroid 4-dehydrogenase 3.
FT                                /FTId=PRO_0000317704.
FT   TRANSMEM     17     37       Potential.
FT   TRANSMEM     81    101       Potential.
FT   TRANSMEM    133    153       Potential.
FT   TRANSMEM    170    190       Potential.
FT   TRANSMEM    207    227       Potential.
FT   TRANSMEM    278    298       Potential.
FT   CONFLICT    109    109       G -> S (in Ref. 2; BAE36955/BAE23944/
FT                                BAC35871/BAC30537).
FT   CONFLICT    298    298       F -> S (in Ref. 2; BAE36955/BAE23944/
FT                                BAC35871/BAC30537).
SQ   SEQUENCE   330 AA;  37966 MW;  16C3A1CEAB415F41 CRC64;
     MAGWAGFELS ALNPLRTLWL ALAAAFLFAL LLQLAPARLL PSCALFQDLL RYGKTKQSGS
     RRPAVCRAFD VPKRYFSHFY VISVVWNGSL LWLLSQSLFL GAPFPNWLGA LLRTLGATQF
     QALEMESKAS RMPAAELALS AFLVLVFLWV HSLRRLFECF YVSVFSNAAI HVVQYCFGLV
     YYVLVGLTVL SQVPMDDKNV YVLGKNLLIQ ARWFHILGMV MFFWSSAHQY KCHVILSNLR
     RNKKGVVIHC QHRIPFGDWF EYVSSANYLA ELMIYISMAV TFGLHNLTWW LVVTYVFFSQ
     ALSAFFNHKF YRSTFVSYPK HRKAFLPFLF
//