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Q9WUP4

- PORED_MOUSE

UniProt

Q9WUP4 - PORED_MOUSE

Protein

Polyprenol reductase

Gene

Srd5a3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 2 (05 Oct 2010)
      Previous versions | rss
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    Functioni

    Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism. Also able to convert testosterone (T) into 5-alpha-dihydrotestosterone (DHT).1 Publication

    Catalytic activityi

    Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.
    A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.

    Pathwayi

    GO - Molecular functioni

    1. 3-oxo-5-alpha-steroid 4-dehydrogenase activity Source: UniProtKB
    2. cholestenone 5-alpha-reductase activity Source: UniProtKB-EC
    3. oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor Source: UniProtKB

    GO - Biological processi

    1. dolichol-linked oligosaccharide biosynthetic process Source: UniProtKB
    2. dolichol metabolic process Source: UniProtKB
    3. polyprenol catabolic process Source: UniProtKB
    4. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    ReactomeiREACT_202113. Synthesis of Dolichyl-phosphate.
    REACT_220332. Androgen biosynthesis.
    UniPathwayiUPA00378.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyprenol reductase (EC:1.3.1.94)
    Alternative name(s):
    3-oxo-5-alpha-steroid 4-dehydrogenase 3 (EC:1.3.1.22)
    Steroid 5-alpha-reductase 2-like
    Steroid 5-alpha-reductase 3
    Short name:
    S5AR 3
    Short name:
    SR type 3
    Gene namesi
    Name:Srd5a3
    Synonyms:Srd5a2l
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1930252. Srd5a3.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Death by E12.5. At E10.5, embryos are smaller and fail to undergo axial rotation observed at E8.5 in wild-types and present dilated hearts and open neural tubes.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 330330Polyprenol reductasePRO_0000317704Add
    BLAST

    Proteomic databases

    PaxDbiQ9WUP4.
    PRIDEiQ9WUP4.

    PTM databases

    PhosphoSiteiQ9WUP4.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9WUP4.
    BgeeiQ9WUP4.
    CleanExiMM_SRD5A3.
    GenevestigatoriQ9WUP4.

    Interactioni

    Protein-protein interaction databases

    BioGridi208270. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9WUP4.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1616CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini38 – 8043LumenalSequence AnalysisAdd
    BLAST
    Topological domaini102 – 13231CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini154 – 16916LumenalSequence AnalysisAdd
    BLAST
    Topological domaini191 – 20616CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini228 – 27750LumenalSequence AnalysisAdd
    BLAST
    Topological domaini299 – 33032CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei17 – 3721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei81 – 10121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei133 – 15321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei170 – 19021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei207 – 22721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei278 – 29821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG330066.
    GeneTreeiENSGT00500000044920.
    HOGENOMiHOG000018885.
    HOVERGENiHBG057797.
    InParanoidiQ3UU82.
    KOiK12345.
    OMAiTSAPEFN.
    OrthoDBiEOG72ZCFT.
    PhylomeDBiQ9WUP4.
    TreeFamiTF315011.

    Family and domain databases

    InterProiIPR001104. 3-oxo-5_a-steroid_4-DH_C.
    [Graphical view]
    PfamiPF02544. Steroid_dh. 1 hit.
    [Graphical view]
    PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9WUP4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAGWAGFELS ALNPLRTLWL ALAAAFLFAL LLQLAPARLL PSCALFQDLL    50
    RYGKTKQSGS RRPAVCRAFD VPKRYFSHFY VISVVWNGSL LWLLSQSLFL 100
    GAPFPNWLSA LLRTLGATQF QALEMESKAS RMPAAELALS AFLVLVFLWV 150
    HSLRRLFECF YVSVFSNAAI HVVQYCFGLV YYVLVGLTVL SQVPMDDKNV 200
    YVLGKNLLIQ ARWFHILGMV MFFWSSAHQY KCHVILSNLR RNKKGVVIHC 250
    QHRIPFGDWF EYVSSANYLA ELMIYISMAV TFGLHNLTWW LVVTYVFSSQ 300
    ALSAFFNHKF YRSTFVSYPK HRKAFLPFLF 330
    Length:330
    Mass (Da):37,936
    Last modified:October 5, 2010 - v2
    Checksum:iA26CAB23B295D75C
    GO
    Isoform 2 (identifier: Q9WUP4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         200-330: VYVLGKNLLI...HRKAFLPFLF → GKWPCQ

    Note: No experimental confirmation available.

    Show »
    Length:205
    Mass (Da):23,032
    Checksum:iFEFAA6DDA00C20D8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti109 – 1091S → G in AAD30567. (PubMed:11116088)Curated
    Sequence conflicti109 – 1091S → G in AAI45648. (PubMed:15489334)Curated
    Sequence conflicti298 – 2981S → F in AAD30567. (PubMed:11116088)Curated
    Sequence conflicti298 – 2981S → F in AAI45648. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei200 – 330131VYVLG…LPFLF → GKWPCQ in isoform 2. 1 PublicationVSP_039779Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF146793 Genomic DNA. Translation: AAD30567.1.
    AK040198 mRNA. Translation: BAC30537.1.
    AK075635 mRNA. Translation: BAC35871.1.
    AK138681 mRNA. Translation: BAE23745.1.
    AK139290 mRNA. Translation: BAE23944.1.
    AK162527 mRNA. Translation: BAE36955.1.
    AC147239 Genomic DNA. No translation available.
    BC145647 mRNA. Translation: AAI45648.1.
    CCDSiCCDS19358.1. [Q9WUP4-1]
    RefSeqiNP_065636.2. NM_020611.4. [Q9WUP4-1]
    UniGeneiMm.289446.

    Genome annotation databases

    EnsembliENSMUST00000031143; ENSMUSP00000031143; ENSMUSG00000029233. [Q9WUP4-1]
    GeneIDi57357.
    KEGGimmu:57357.
    UCSCiuc008xun.2. mouse. [Q9WUP4-2]
    uc008xuo.2. mouse. [Q9WUP4-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF146793 Genomic DNA. Translation: AAD30567.1 .
    AK040198 mRNA. Translation: BAC30537.1 .
    AK075635 mRNA. Translation: BAC35871.1 .
    AK138681 mRNA. Translation: BAE23745.1 .
    AK139290 mRNA. Translation: BAE23944.1 .
    AK162527 mRNA. Translation: BAE36955.1 .
    AC147239 Genomic DNA. No translation available.
    BC145647 mRNA. Translation: AAI45648.1 .
    CCDSi CCDS19358.1. [Q9WUP4-1 ]
    RefSeqi NP_065636.2. NM_020611.4. [Q9WUP4-1 ]
    UniGenei Mm.289446.

    3D structure databases

    ProteinModelPortali Q9WUP4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 208270. 1 interaction.

    PTM databases

    PhosphoSitei Q9WUP4.

    Proteomic databases

    PaxDbi Q9WUP4.
    PRIDEi Q9WUP4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000031143 ; ENSMUSP00000031143 ; ENSMUSG00000029233 . [Q9WUP4-1 ]
    GeneIDi 57357.
    KEGGi mmu:57357.
    UCSCi uc008xun.2. mouse. [Q9WUP4-2 ]
    uc008xuo.2. mouse. [Q9WUP4-1 ]

    Organism-specific databases

    CTDi 79644.
    MGIi MGI:1930252. Srd5a3.

    Phylogenomic databases

    eggNOGi NOG330066.
    GeneTreei ENSGT00500000044920.
    HOGENOMi HOG000018885.
    HOVERGENi HBG057797.
    InParanoidi Q3UU82.
    KOi K12345.
    OMAi TSAPEFN.
    OrthoDBi EOG72ZCFT.
    PhylomeDBi Q9WUP4.
    TreeFami TF315011.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_202113. Synthesis of Dolichyl-phosphate.
    REACT_220332. Androgen biosynthesis.

    Miscellaneous databases

    NextBioi 313740.
    PROi Q9WUP4.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9WUP4.
    Bgeei Q9WUP4.
    CleanExi MM_SRD5A3.
    Genevestigatori Q9WUP4.

    Family and domain databases

    InterProi IPR001104. 3-oxo-5_a-steroid_4-DH_C.
    [Graphical view ]
    Pfami PF02544. Steroid_dh. 1 hit.
    [Graphical view ]
    PROSITEi PS50244. S5A_REDUCTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The mouse Clock locus: sequence and comparative analysis of 204 kb from mouse chromosome 5."
      Wilsbacher L.D., Sangoram A.M., Antoch M.P., Takahashi J.S.
      Genome Res. 10:1928-1940(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/Sv.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J.
      Tissue: Cerebellum, Thymus and Urinary bladder.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    5. Cited for: FUNCTION, PATHWAY, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiPORED_MOUSE
    AccessioniPrimary (citable) accession number: Q9WUP4
    Secondary accession number(s): D3YZB6
    , D3Z3J5, Q3UU82, Q8BGE3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: October 5, 2010
    Last modified: October 1, 2014
    This is version 87 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3