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Q9WUP4

- PORED_MOUSE

UniProt

Q9WUP4 - PORED_MOUSE

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Protein
Polyprenol reductase
Gene
Srd5a3, Srd5a2l
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism. Also able to convert testosterone (T) into 5-alpha-dihydrotestosterone (DHT).1 Publication

Catalytic activityi

Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.
A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.

Pathwayi

GO - Molecular functioni

  1. 3-oxo-5-alpha-steroid 4-dehydrogenase activity Source: UniProtKB
  2. cholestenone 5-alpha-reductase activity Source: UniProtKB-EC
  3. oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor Source: UniProtKB

GO - Biological processi

  1. dolichol metabolic process Source: UniProtKB
  2. dolichol-linked oligosaccharide biosynthetic process Source: UniProtKB
  3. polyprenol catabolic process Source: UniProtKB
  4. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiREACT_202113. Synthesis of Dolichyl-phosphate.
REACT_220332. Androgen biosynthesis.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyprenol reductase (EC:1.3.1.94)
Alternative name(s):
3-oxo-5-alpha-steroid 4-dehydrogenase 3 (EC:1.3.1.22)
Steroid 5-alpha-reductase 2-like
Steroid 5-alpha-reductase 3
Short name:
S5AR 3
Short name:
SR type 3
Gene namesi
Name:Srd5a3
Synonyms:Srd5a2l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:1930252. Srd5a3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1616Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei17 – 3721Helical; Reviewed prediction
Add
BLAST
Topological domaini38 – 8043Lumenal Reviewed prediction
Add
BLAST
Transmembranei81 – 10121Helical; Reviewed prediction
Add
BLAST
Topological domaini102 – 13231Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei133 – 15321Helical; Reviewed prediction
Add
BLAST
Topological domaini154 – 16916Lumenal Reviewed prediction
Add
BLAST
Transmembranei170 – 19021Helical; Reviewed prediction
Add
BLAST
Topological domaini191 – 20616Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei207 – 22721Helical; Reviewed prediction
Add
BLAST
Topological domaini228 – 27750Lumenal Reviewed prediction
Add
BLAST
Transmembranei278 – 29821Helical; Reviewed prediction
Add
BLAST
Topological domaini299 – 33032Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Death by E12.5. At E10.5, embryos are smaller and fail to undergo axial rotation observed at E8.5 in wild-types and present dilated hearts and open neural tubes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 330330Polyprenol reductase
PRO_0000317704Add
BLAST

Proteomic databases

PaxDbiQ9WUP4.
PRIDEiQ9WUP4.

PTM databases

PhosphoSiteiQ9WUP4.

Expressioni

Gene expression databases

ArrayExpressiQ9WUP4.
BgeeiQ9WUP4.
CleanExiMM_SRD5A3.
GenevestigatoriQ9WUP4.

Interactioni

Protein-protein interaction databases

BioGridi208270. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9WUP4.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG330066.
GeneTreeiENSGT00500000044920.
HOGENOMiHOG000018885.
HOVERGENiHBG057797.
InParanoidiQ3UU82.
KOiK12345.
OMAiTSAPEFN.
OrthoDBiEOG72ZCFT.
PhylomeDBiQ9WUP4.
TreeFamiTF315011.

Family and domain databases

InterProiIPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamiPF02544. Steroid_dh. 1 hit.
[Graphical view]
PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9WUP4-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAGWAGFELS ALNPLRTLWL ALAAAFLFAL LLQLAPARLL PSCALFQDLL    50
RYGKTKQSGS RRPAVCRAFD VPKRYFSHFY VISVVWNGSL LWLLSQSLFL 100
GAPFPNWLSA LLRTLGATQF QALEMESKAS RMPAAELALS AFLVLVFLWV 150
HSLRRLFECF YVSVFSNAAI HVVQYCFGLV YYVLVGLTVL SQVPMDDKNV 200
YVLGKNLLIQ ARWFHILGMV MFFWSSAHQY KCHVILSNLR RNKKGVVIHC 250
QHRIPFGDWF EYVSSANYLA ELMIYISMAV TFGLHNLTWW LVVTYVFSSQ 300
ALSAFFNHKF YRSTFVSYPK HRKAFLPFLF 330
Length:330
Mass (Da):37,936
Last modified:October 5, 2010 - v2
Checksum:iA26CAB23B295D75C
GO
Isoform 2 (identifier: Q9WUP4-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     200-330: VYVLGKNLLI...HRKAFLPFLF → GKWPCQ

Note: No experimental confirmation available.

Show »
Length:205
Mass (Da):23,032
Checksum:iFEFAA6DDA00C20D8
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei200 – 330131VYVLG…LPFLF → GKWPCQ in isoform 2.
VSP_039779Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti109 – 1091S → G in AAD30567. 1 Publication
Sequence conflicti109 – 1091S → G in AAI45648. 1 Publication
Sequence conflicti298 – 2981S → F in AAD30567. 1 Publication
Sequence conflicti298 – 2981S → F in AAI45648. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF146793 Genomic DNA. Translation: AAD30567.1.
AK040198 mRNA. Translation: BAC30537.1.
AK075635 mRNA. Translation: BAC35871.1.
AK138681 mRNA. Translation: BAE23745.1.
AK139290 mRNA. Translation: BAE23944.1.
AK162527 mRNA. Translation: BAE36955.1.
AC147239 Genomic DNA. No translation available.
BC145647 mRNA. Translation: AAI45648.1.
CCDSiCCDS19358.1. [Q9WUP4-1]
RefSeqiNP_065636.2. NM_020611.4. [Q9WUP4-1]
UniGeneiMm.289446.

Genome annotation databases

EnsembliENSMUST00000031143; ENSMUSP00000031143; ENSMUSG00000029233. [Q9WUP4-1]
GeneIDi57357.
KEGGimmu:57357.
UCSCiuc008xun.2. mouse. [Q9WUP4-2]
uc008xuo.2. mouse. [Q9WUP4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF146793 Genomic DNA. Translation: AAD30567.1 .
AK040198 mRNA. Translation: BAC30537.1 .
AK075635 mRNA. Translation: BAC35871.1 .
AK138681 mRNA. Translation: BAE23745.1 .
AK139290 mRNA. Translation: BAE23944.1 .
AK162527 mRNA. Translation: BAE36955.1 .
AC147239 Genomic DNA. No translation available.
BC145647 mRNA. Translation: AAI45648.1 .
CCDSi CCDS19358.1. [Q9WUP4-1 ]
RefSeqi NP_065636.2. NM_020611.4. [Q9WUP4-1 ]
UniGenei Mm.289446.

3D structure databases

ProteinModelPortali Q9WUP4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 208270. 1 interaction.

PTM databases

PhosphoSitei Q9WUP4.

Proteomic databases

PaxDbi Q9WUP4.
PRIDEi Q9WUP4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000031143 ; ENSMUSP00000031143 ; ENSMUSG00000029233 . [Q9WUP4-1 ]
GeneIDi 57357.
KEGGi mmu:57357.
UCSCi uc008xun.2. mouse. [Q9WUP4-2 ]
uc008xuo.2. mouse. [Q9WUP4-1 ]

Organism-specific databases

CTDi 79644.
MGIi MGI:1930252. Srd5a3.

Phylogenomic databases

eggNOGi NOG330066.
GeneTreei ENSGT00500000044920.
HOGENOMi HOG000018885.
HOVERGENi HBG057797.
InParanoidi Q3UU82.
KOi K12345.
OMAi TSAPEFN.
OrthoDBi EOG72ZCFT.
PhylomeDBi Q9WUP4.
TreeFami TF315011.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_202113. Synthesis of Dolichyl-phosphate.
REACT_220332. Androgen biosynthesis.

Miscellaneous databases

NextBioi 313740.
PROi Q9WUP4.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9WUP4.
Bgeei Q9WUP4.
CleanExi MM_SRD5A3.
Genevestigatori Q9WUP4.

Family and domain databases

InterProi IPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view ]
Pfami PF02544. Steroid_dh. 1 hit.
[Graphical view ]
PROSITEi PS50244. S5A_REDUCTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The mouse Clock locus: sequence and comparative analysis of 204 kb from mouse chromosome 5."
    Wilsbacher L.D., Sangoram A.M., Antoch M.P., Takahashi J.S.
    Genome Res. 10:1928-1940(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Cerebellum, Thymus and Urinary bladder.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  5. Cited for: FUNCTION, PATHWAY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiPORED_MOUSE
AccessioniPrimary (citable) accession number: Q9WUP4
Secondary accession number(s): D3YZB6
, D3Z3J5, Q3UU82, Q8BGE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 5, 2010
Last modified: September 3, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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