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Protein

Polyprenol reductase

Gene

Srd5a3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism. Also able to convert testosterone (T) into 5-alpha-dihydrotestosterone (DHT).1 Publication

Catalytic activityi

Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.
A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta4-steroid + NADPH.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • dolichol-linked oligosaccharide biosynthetic process Source: UniProtKB
  • dolichol metabolic process Source: UniProtKB
  • polyprenol catabolic process Source: UniProtKB

Keywordsi

Molecular functionOxidoreductase
LigandNADP

Enzyme and pathway databases

BRENDAi1.3.1.94 3474
ReactomeiR-MMU-193048 Androgen biosynthesis
R-MMU-446199 Synthesis of Dolichyl-phosphate
UniPathwayiUPA00378

Names & Taxonomyi

Protein namesi
Recommended name:
Polyprenol reductase (EC:1.3.1.94)
Alternative name(s):
3-oxo-5-alpha-steroid 4-dehydrogenase 3 (EC:1.3.1.22)
Steroid 5-alpha-reductase 2-like
Steroid 5-alpha-reductase 3
Short name:
S5AR 3
Short name:
SR type 3
Gene namesi
Name:Srd5a3
Synonyms:Srd5a2l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1930252 Srd5a3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 16CytoplasmicSequence analysisAdd BLAST16
Transmembranei17 – 37HelicalSequence analysisAdd BLAST21
Topological domaini38 – 80LumenalSequence analysisAdd BLAST43
Transmembranei81 – 101HelicalSequence analysisAdd BLAST21
Topological domaini102 – 132CytoplasmicSequence analysisAdd BLAST31
Transmembranei133 – 153HelicalSequence analysisAdd BLAST21
Topological domaini154 – 169LumenalSequence analysisAdd BLAST16
Transmembranei170 – 190HelicalSequence analysisAdd BLAST21
Topological domaini191 – 206CytoplasmicSequence analysisAdd BLAST16
Transmembranei207 – 227HelicalSequence analysisAdd BLAST21
Topological domaini228 – 277LumenalSequence analysisAdd BLAST50
Transmembranei278 – 298HelicalSequence analysisAdd BLAST21
Topological domaini299 – 330CytoplasmicSequence analysisAdd BLAST32

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Death by E12.5. At E10.5, embryos are smaller and fail to undergo axial rotation observed at E8.5 in wild-types and present dilated hearts and open neural tubes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003177041 – 330Polyprenol reductaseAdd BLAST330

Proteomic databases

PaxDbiQ9WUP4
PeptideAtlasiQ9WUP4
PRIDEiQ9WUP4

PTM databases

iPTMnetiQ9WUP4
PhosphoSitePlusiQ9WUP4
SwissPalmiQ9WUP4

Expressioni

Gene expression databases

BgeeiENSMUSG00000029233
CleanExiMM_SRD5A3
ExpressionAtlasiQ9WUP4 baseline and differential
GenevisibleiQ9WUP4 MM

Interactioni

Protein-protein interaction databases

BioGridi208270, 2 interactors
STRINGi10090.ENSMUSP00000031143

Structurei

3D structure databases

ProteinModelPortaliQ9WUP4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1640 Eukaryota
ENOG4111JQM LUCA
GeneTreeiENSGT00500000044920
HOGENOMiHOG000018885
HOVERGENiHBG057797
InParanoidiQ9WUP4
KOiK12345
OMAiRRFYETN
OrthoDBiEOG091G0F09
PhylomeDBiQ9WUP4
TreeFamiTF315011

Family and domain databases

InterProiView protein in InterPro
IPR001104 3-oxo-5_a-steroid_4-DH_C
PfamiView protein in Pfam
PF02544 Steroid_dh, 1 hit
PROSITEiView protein in PROSITE
PS50244 S5A_REDUCTASE, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9WUP4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGWAGFELS ALNPLRTLWL ALAAAFLFAL LLQLAPARLL PSCALFQDLL
60 70 80 90 100
RYGKTKQSGS RRPAVCRAFD VPKRYFSHFY VISVVWNGSL LWLLSQSLFL
110 120 130 140 150
GAPFPNWLSA LLRTLGATQF QALEMESKAS RMPAAELALS AFLVLVFLWV
160 170 180 190 200
HSLRRLFECF YVSVFSNAAI HVVQYCFGLV YYVLVGLTVL SQVPMDDKNV
210 220 230 240 250
YVLGKNLLIQ ARWFHILGMV MFFWSSAHQY KCHVILSNLR RNKKGVVIHC
260 270 280 290 300
QHRIPFGDWF EYVSSANYLA ELMIYISMAV TFGLHNLTWW LVVTYVFSSQ
310 320 330
ALSAFFNHKF YRSTFVSYPK HRKAFLPFLF
Length:330
Mass (Da):37,936
Last modified:October 5, 2010 - v2
Checksum:iA26CAB23B295D75C
GO
Isoform 2 (identifier: Q9WUP4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     200-330: VYVLGKNLLI...HRKAFLPFLF → GKWPCQ

Note: No experimental confirmation available.
Show »
Length:205
Mass (Da):23,032
Checksum:iFEFAA6DDA00C20D8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti109S → G in AAD30567 (PubMed:11116088).Curated1
Sequence conflicti109S → G in AAI45648 (PubMed:15489334).Curated1
Sequence conflicti298S → F in AAD30567 (PubMed:11116088).Curated1
Sequence conflicti298S → F in AAI45648 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_039779200 – 330VYVLG…LPFLF → GKWPCQ in isoform 2. 1 PublicationAdd BLAST131

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF146793 Genomic DNA Translation: AAD30567.1
AK040198 mRNA Translation: BAC30537.1
AK075635 mRNA Translation: BAC35871.1
AK138681 mRNA Translation: BAE23745.1
AK139290 mRNA Translation: BAE23944.1
AK162527 mRNA Translation: BAE36955.1
AC147239 Genomic DNA No translation available.
BC145647 mRNA Translation: AAI45648.1
CCDSiCCDS19358.1 [Q9WUP4-1]
RefSeqiNP_065636.2, NM_020611.4 [Q9WUP4-1]
UniGeneiMm.289446

Genome annotation databases

EnsembliENSMUST00000031143; ENSMUSP00000031143; ENSMUSG00000029233 [Q9WUP4-1]
GeneIDi57357
KEGGimmu:57357
UCSCiuc008xun.2 mouse [Q9WUP4-2]
uc008xuo.2 mouse [Q9WUP4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPORED_MOUSE
AccessioniPrimary (citable) accession number: Q9WUP4
Secondary accession number(s): D3YZB6
, D3Z3J5, Q3UU82, Q8BGE3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 5, 2010
Last modified: May 23, 2018
This is version 110 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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