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Protein

Receptor activity-modifying protein 2

Gene

Ramp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. Acts as a receptor for adrenomedullin (AM) together with CALCRL.1 Publication

GO - Molecular functioni

  • coreceptor activity Source: MGI
  • protein transporter activity Source: UniProtKB

GO - Biological processi

  • adherens junction assembly Source: UniProtKB
  • angiogenesis Source: UniProtKB
  • basement membrane assembly Source: UniProtKB
  • bicellular tight junction assembly Source: UniProtKB
  • calcium ion transport Source: UniProtKB
  • cAMP biosynthetic process Source: UniProtKB
  • cellular response to hormone stimulus Source: Ensembl
  • cellular response to vascular endothelial growth factor stimulus Source: UniProtKB
  • female pregnancy Source: Ensembl
  • G-protein coupled receptor signaling pathway Source: MGI
  • heart development Source: UniProtKB
  • intracellular protein transport Source: InterPro
  • negative regulation of endothelial cell apoptotic process Source: UniProtKB
  • negative regulation of vascular permeability Source: UniProtKB
  • positive regulation of angiogenesis Source: UniProtKB
  • positive regulation of cAMP biosynthetic process Source: MGI
  • positive regulation of gene expression Source: UniProtKB
  • positive regulation of vasculogenesis Source: UniProtKB
  • protein localization to plasma membrane Source: UniProtKB
  • protein transport Source: UniProtKB
  • receptor internalization Source: UniProtKB
  • regulation of blood pressure Source: UniProtKB
  • regulation of G-protein coupled receptor protein signaling pathway Source: InterPro
  • response to estradiol Source: Ensembl
  • response to hypoxia Source: Ensembl
  • response to progesterone Source: Ensembl
  • sprouting angiogenesis Source: UniProtKB
  • vascular smooth muscle cell development Source: UniProtKB
  • vasculogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiR-MMU-418555. G alpha (s) signalling events.
R-MMU-419812. Calcitonin-like ligand receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor activity-modifying protein 2
Gene namesi
Name:Ramp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1859650. Ramp2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini45 – 159115ExtracellularSequence analysisAdd
BLAST
Transmembranei160 – 18021HelicalSequence analysisAdd
BLAST
Topological domaini181 – 1899CytoplasmicSequence analysis

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4444Sequence analysisAdd
BLAST
Chaini45 – 189145Receptor activity-modifying protein 2PRO_0000030173Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi50 – 501N-linked (GlcNAc...)Sequence analysis
Glycosylationi58 – 581N-linked (GlcNAc...)Sequence analysis
Disulfide bondi83 ↔ 113By similarity
Disulfide bondi98 ↔ 145By similarity
Glycosylationi99 – 991N-linked (GlcNAc...)Sequence analysis
Glycosylationi144 – 1441N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9WUP0.
PaxDbiQ9WUP0.
PRIDEiQ9WUP0.

PTM databases

PhosphoSiteiQ9WUP0.

Expressioni

Tissue specificityi

Ubiquitous. Expressed predominantly in embryonic brain, lung and gut and in adult heart, lung, skeletal muscle and brain.2 Publications

Gene expression databases

BgeeiQ9WUP0.
ExpressionAtlasiQ9WUP0. baseline and differential.
GenevisibleiQ9WUP0. MM.

Interactioni

Subunit structurei

Heterodimer of CALCRL and RAMP2.

Protein-protein interaction databases

IntActiQ9WUP0. 1 interaction.
MINTiMINT-4131787.
STRINGi10090.ENSMUSP00000122072.

Structurei

3D structure databases

ProteinModelPortaliQ9WUP0.
SMRiQ9WUP0. Positions 71-153.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RAMP family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IYPR. Eukaryota.
ENOG4112A9G. LUCA.
GeneTreeiENSGT00390000016200.
HOVERGENiHBG067366.
InParanoidiQ9WUP0.
KOiK08448.
OMAiMESYERT.
OrthoDBiEOG7JT6Z9.
PhylomeDBiQ9WUP0.
TreeFamiTF333286.

Family and domain databases

InterProiIPR006985. RAMP.
[Graphical view]
PANTHERiPTHR14076. PTHR14076. 1 hit.
PfamiPF04901. RAMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9WUP0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPLRVERAP GGSRLGVTRA QRPTALCLPP LLLLLLLLLG AVSASPESLN
60 70 80 90 100
QSLPESQNQS HPTEDSLVSK GKMEDYETHV LPCWYEYKSC MDSVKDWCNW
110 120 130 140 150
TLISRHYSDL QNCLEYNADK FGLGFPNPLA ENIILEAHLI HFANCSLVQP
160 170 180
TFSDPPEDVL LAMIIAPICL IPFLVTLVVW RSKDSDAQA
Length:189
Mass (Da):21,017
Last modified:November 1, 1999 - v1
Checksum:iCEE51D9FE9751F10
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti10 – 101P → T in AAH69992 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF146523 mRNA. Translation: AAD35019.1.
AJ250490 mRNA. Translation: CAB59512.1.
AF209906 mRNA. Translation: AAF21038.1.
AK003810 mRNA. Translation: BAB23009.1.
BC069992 mRNA. Translation: AAH69992.1.
CCDSiCCDS25457.1.
PIRiJC7262.
RefSeqiNP_062317.1. NM_019444.2.
UniGeneiMm.260698.
Mm.298256.

Genome annotation databases

EnsembliENSMUST00000129680; ENSMUSP00000122072; ENSMUSG00000001240.
GeneIDi54409.
KEGGimmu:54409.
UCSCiuc007lny.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF146523 mRNA. Translation: AAD35019.1.
AJ250490 mRNA. Translation: CAB59512.1.
AF209906 mRNA. Translation: AAF21038.1.
AK003810 mRNA. Translation: BAB23009.1.
BC069992 mRNA. Translation: AAH69992.1.
CCDSiCCDS25457.1.
PIRiJC7262.
RefSeqiNP_062317.1. NM_019444.2.
UniGeneiMm.260698.
Mm.298256.

3D structure databases

ProteinModelPortaliQ9WUP0.
SMRiQ9WUP0. Positions 71-153.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9WUP0. 1 interaction.
MINTiMINT-4131787.
STRINGi10090.ENSMUSP00000122072.

PTM databases

PhosphoSiteiQ9WUP0.

Proteomic databases

MaxQBiQ9WUP0.
PaxDbiQ9WUP0.
PRIDEiQ9WUP0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000129680; ENSMUSP00000122072; ENSMUSG00000001240.
GeneIDi54409.
KEGGimmu:54409.
UCSCiuc007lny.1. mouse.

Organism-specific databases

CTDi10266.
MGIiMGI:1859650. Ramp2.

Phylogenomic databases

eggNOGiENOG410IYPR. Eukaryota.
ENOG4112A9G. LUCA.
GeneTreeiENSGT00390000016200.
HOVERGENiHBG067366.
InParanoidiQ9WUP0.
KOiK08448.
OMAiMESYERT.
OrthoDBiEOG7JT6Z9.
PhylomeDBiQ9WUP0.
TreeFamiTF333286.

Enzyme and pathway databases

ReactomeiR-MMU-418555. G alpha (s) signalling events.
R-MMU-419812. Calcitonin-like ligand receptors.

Miscellaneous databases

ChiTaRSiRamp2. mouse.
NextBioi311276.
PROiQ9WUP0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9WUP0.
ExpressionAtlasiQ9WUP0. baseline and differential.
GenevisibleiQ9WUP0. MM.

Family and domain databases

InterProiIPR006985. RAMP.
[Graphical view]
PANTHERiPTHR14076. PTHR14076. 1 hit.
PfamiPF04901. RAMP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of mouse CGRP/adrenomedullin receptor subunits."
    Derst C., Preisig-Mueller R., Gerhardus J., Daut J.
    Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Mouse receptor-activity-modifying proteins 1, -2 and -3: amino acid sequence, expression and function."
    Husmann K., Sexton P.M., Fischer J.A., Born W.
    Mol. Cell. Endocrinol. 162:35-43(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
  3. "Decreased gene expression of adrenomedullin receptor in mouse lungs during sepsis."
    Ono Y., Okano I., Kojima M., Okada K., Kangawa K.
    Biochem. Biophys. Res. Commun. 271:197-202(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: C57BL/6J.
    Tissue: Lung.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Jaw and Limb.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Lung.

Entry informationi

Entry nameiRAMP2_MOUSE
AccessioniPrimary (citable) accession number: Q9WUP0
Secondary accession number(s): Q6IS25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: November 1, 1999
Last modified: May 11, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.