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Protein

Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial

Gene

Suclg1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and specificity for either ATP or GTP is provided by different beta subunits.UniRule annotation

Catalytic activityi

ATP + succinate + CoA = ADP + phosphate + succinyl-CoA.UniRule annotation
GTP + succinate + CoA = GDP + phosphate + succinyl-CoA.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes succinate from succinyl-CoA (ligase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial (Suclg2), Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (Sucla2), Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial (Suclg2), Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial (Suclg1)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes succinate from succinyl-CoA (ligase route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei90Coenzyme AUniRule annotation1
Binding sitei207Substrate; shared with subunit betaUniRule annotation1
Active sitei299Tele-phosphohistidine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processTricarboxylic acid cycle
LigandNucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-71403 Citric acid cycle (TCA cycle)
UniPathwayiUPA00223; UER00999

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrialUniRule annotation (EC:6.2.1.4UniRule annotation, EC:6.2.1.5UniRule annotation)
Alternative name(s):
Succinyl-CoA synthetase subunit alphaUniRule annotation
Short name:
SCS-alphaUniRule annotation
Gene namesi
Name:Suclg1UniRule annotation
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1927234 Suclg1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 34MitochondrionUniRule annotationAdd BLAST34
ChainiPRO_000003334135 – 346Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrialUniRule annotationAdd BLAST312

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei54N6-acetyllysineCombined sources1
Modified residuei57N6-acetyllysine; alternateCombined sources1
Modified residuei57N6-succinyllysine; alternateCombined sources1
Modified residuei66N6-acetyllysine; alternateCombined sources1
Modified residuei66N6-succinyllysine; alternateCombined sources1
Modified residuei81N6-acetyllysineCombined sources1
Modified residuei94N6-acetyllysineCombined sources1
Modified residuei105N6-acetyllysineCombined sources1
Modified residuei338N6-succinyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9WUM5
MaxQBiQ9WUM5
PaxDbiQ9WUM5
PeptideAtlasiQ9WUM5
PRIDEiQ9WUM5

PTM databases

iPTMnetiQ9WUM5
PhosphoSitePlusiQ9WUM5
SwissPalmiQ9WUM5

Expressioni

Gene expression databases

BgeeiENSMUSG00000052738
CleanExiMM_SUCLG1
ExpressionAtlasiQ9WUM5 baseline and differential
GenevisibleiQ9WUM5 MM

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. Different beta subunits determine nucleotide specificity. Together with the ATP-specific beta subunit SUCLA2, forms an ADP-forming succinyl-CoA synthetase (A-SCS). Together with the GTP-specific beta subunit SUCLG2 forms a GDP-forming succinyl-CoA synthetase (G-SCS).UniRule annotation

Protein-protein interaction databases

BioGridi207990, 2 interactors
CORUMiQ9WUM5
IntActiQ9WUM5, 6 interactors
MINTiQ9WUM5
STRINGi10090.ENSMUSP00000065113

Structurei

3D structure databases

ProteinModelPortaliQ9WUM5
SMRiQ9WUM5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni64 – 67Coenzyme A bindingUniRule annotation4
Regioni143 – 145Coenzyme A bindingUniRule annotation3

Sequence similaritiesi

Belongs to the succinate/malate CoA ligase alpha subunit family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1255 Eukaryota
COG0074 LUCA
GeneTreeiENSGT00530000063275
HOGENOMiHOG000239685
HOVERGENiHBG000957
InParanoidiQ9WUM5
KOiK01899
OMAiIIFVPPA
OrthoDBiEOG091G0D9C
PhylomeDBiQ9WUM5
TreeFamiTF300666

Family and domain databases

Gene3Di3.40.50.261, 1 hit
HAMAPiMF_01988 Succ_CoA_alpha, 1 hit
InterProiView protein in InterPro
IPR017440 Cit_synth/succinyl-CoA_lig_AS
IPR033847 Citrt_syn/SCS-alpha_CS
IPR003781 CoA-bd
IPR005810 CoA_lig_alpha
IPR005811 CoA_ligase
IPR036291 NAD(P)-bd_dom_sf
IPR016102 Succinyl-CoA_synth-like
PfamiView protein in Pfam
PF02629 CoA_binding, 1 hit
PF00549 Ligase_CoA, 1 hit
PIRSFiPIRSF001553 SucCS_alpha, 1 hit
SMARTiView protein in SMART
SM00881 CoA_binding, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF52210 SSF52210, 1 hit
TIGRFAMsiTIGR01019 sucCoAalpha, 1 hit
PROSITEiView protein in PROSITE
PS01216 SUCCINYL_COA_LIG_1, 1 hit
PS00399 SUCCINYL_COA_LIG_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9WUM5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTATVVAAAA TATMVSSSSG LAAARLLSRT FLLQQNGIRH GSYTASRKHI
60 70 80 90 100
YIDKNTKIIC QGFTGKQGTF HSQQALEYGT KLVGGTTPGK GGQKHLGLPV
110 120 130 140 150
FNTVKEAKEK TGATASVIYV PPPFAAAAIN EAIDAEIPLV VCITEGIPQQ
160 170 180 190 200
DMVRVKHRLT RQGTTRLIGP NCPGVINPGE CKIGIMPGHI HKKGRIGIVS
210 220 230 240 250
RSGTLTYEAV HQTTQVGLGQ SLCIGIGGDP FNGTDFIDCL EVFLNDPATE
260 270 280 290 300
GIILIGEIGG HAEENAAAFL KEHNSGPKAK PVVSFIAGIT APPGRRMGHA
310 320 330 340
GAIIAGGKGG AKEKISALQS AGVVVSMSPA QLGTTIYKEF EKRKML
Length:346
Mass (Da):36,155
Last modified:February 10, 2009 - v4
Checksum:i1EB7A444DFCE32FA
GO

Sequence cautioni

The sequence AAD33927 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH11087 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB22331 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB23804 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC40634 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6V → L in AAD33927 (Ref. 2) Curated1
Sequence conflicti175V → I in BAC40634 (PubMed:16141072).Curated1
Sequence conflicti188G → A in BAB22331 (PubMed:16141072).Curated1
Sequence conflicti201R → K in AAD33927 (Ref. 2) Curated1
Sequence conflicti220Q → H in AAD33927 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002754 mRNA Translation: BAB22331.1 Different initiation.
AK005080 mRNA Translation: BAB23804.1 Different initiation.
AK088888 mRNA Translation: BAC40634.1 Different initiation.
AF144101 mRNA Translation: AAD33927.2 Different initiation.
BC011087 mRNA Translation: AAH11087.1 Different initiation.
CCDSiCCDS20246.2
RefSeqiNP_063932.2, NM_019879.3
UniGeneiMm.29845

Genome annotation databases

EnsembliENSMUST00000064740; ENSMUSP00000065113; ENSMUSG00000052738
GeneIDi56451
KEGGimmu:56451
UCSCiuc009cjm.2 mouse

Similar proteinsi

Entry informationi

Entry nameiSUCA_MOUSE
AccessioniPrimary (citable) accession number: Q9WUM5
Secondary accession number(s): Q8C2C3, Q9DBA3, Q9DCI8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: February 10, 2009
Last modified: May 23, 2018
This is version 170 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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