Reviewed, UniProtKB/Swiss-Prot Q9WUL7 (ARL3_MOUSE)

Names and origin

Protein names

Recommended name:
    ADP-ribosylation factor-like protein 3

Gene names

Name:

Arl3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	182 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for normal cytokinesis. Does not act as an allosteric activator of the cholera toxin catalytic subunit By similarity.
Subunit structure	Interacts with RP2, SYS1 and SCOC By similarity.
Subcellular location	Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Spindle. Nucleus. Note: Present on the mitotic spindle By similarity.
Sequence similarities	Belongs to the small GTPase superfamily. Arf family.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Golgi apparatus Membrane Nucleus
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
PTM	Lipoprotein Myristate
Technical term	3D-structure
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell spindle Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Ref.1 Inferred from direct assay. Source: MGI magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Potential

Chain

2 – 182

181

ADP-ribosylation factor-like protein 3

PRO_0000207457

Regions

Nucleotide binding

24 – 31

8

GTP

Nucleotide binding

67 – 71

5

GTP By similarity

Nucleotide binding

126 – 129

4

GTP

Nucleotide binding

159 – 161

3

GTP

Sites

Metal binding

31

1

Magnesium

Metal binding

48

1

Magnesium

Binding site

48

1

GTP

Binding site

70

1

GTP; via amide nitrogen

Amino acid modifications

Lipidation

2

1

N-myristoyl glycine Potential

Secondary structure

1

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182

Helix Strand Turn

Details...

Helix

6 – 9

4

Beta strand

18 – 25

8

Helix

30 – 37

8

Beta strand

43 – 48

6

Beta strand

51 – 58

8

Beta strand

61 – 67

7

Helix

72 – 74

3

Helix

75 – 82

8

Beta strand

86 – 93

8

Helix

97 – 99

3

Helix

100 – 110

11

Helix

114 – 116

3

Beta strand

121 – 126

6

Helix

136 – 142

7

Helix

145 – 147

3

Beta strand

153 – 157

5

Turn

160 – 162

3

Helix

166 – 175

10

Sequences

	Sequence		Length	Mass (Da)
	Q9WUL7-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 16B85B2268ADB9DE	FASTA	182	20,487
	10 20 30 40 50 60 MGLLSILRKL KSAPDQEVRI LLLGLDNAGK TTLLKQLASE DISHITPTQG FNIKSVQSQG 70 80 90 100 110 120 FKLNVWDIGG QRKIRPYWRS YFENTDILIY VIDSADRKRF EETGQELTEL LEEEKLSCVP 130 140 150 160 170 180 VLIFANKQDL LTAAPASEIA EGLNLHTIRD RVWQIQSCSA LTGEGVQDGM NWVCKNVNAK KK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The delta subunit of rod specific cyclic GMP phosphodiesterase, PDE delta, interacts with the Arf-like protein Arl3 in a GTP specific manner." Linari M., Hanzal-Bayer M., Becker J. FEBS Lett. 458:55-59(1999) [PubMed: 10518933] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye.
[3]	"Structural and biochemical properties show ARL3-GDP as a distinct GTP binding protein." Hillig R.C., Hanzal-Bayer M., Linari M., Becker J., Wittinghofer A., Renault L. Structure 8:1239-1245(2000) [PubMed: 11188688] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) IN COMPLEX WITH GDP AND MAGNESIUM IONS.
[4]	"The retinitis pigmentosa 2 gene product is a GTPase-activating protein for Arf-like 3." Veltel S., Gasper R., Eisenacher E., Wittinghofer A. Nat. Struct. Mol. Biol. 15:373-380(2008) [PubMed: 18376416] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 17-177, INTERACTION WITH RP2.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF143241 mRNA. Translation: AAD33067.1.
BC042941 mRNA. Translation: AAH42941.1.

IPI

IPI00124787.

RefSeq

NP_062692.1.

UniGene

Mm.22085

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1FZQ	X-ray	1.70	A	2-182	[»]
3BH6	X-ray	2.60	A	17-177	[»]
3BH7	X-ray	1.90	A	17-177	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:29025N.

2-D gel databases

REPRODUCTION-2DPAGE

Q9WUL7.

Proteomic databases

PRIDE

Q9WUL7.

Genome annotation databases

Ensembl

ENSMUSG00000025035. Mus musculus. [Contig view]

GeneID

56350.

KEGG

mmu:56350.

Organism-specific databases

MGI

MGI:1929699. Arl3.

Phylogenomic databases

HOGENOM

Q9WUL7.

HOVERGEN

Q9WUL7.

OMA

Q9WUL7. PTAGFNI.

Gene expression databases

ArrayExpress

Q9WUL7.

Bgee

Q9WUL7.

CleanEx

MM_ARL3.

GermOnline

ENSMUSG00000025035. Mus musculus.

Family and domain databases

InterPro

IPR006688. ARF.
IPR006689. ARF/SAR.
IPR005225. Small_GTP_bd.
[Graphical view]

PANTHER

PTHR11711. ARF/SAR. 1 hit.

Pfam

PF00025. Arf. 1 hit.
[Graphical view]

PRINTS

PR00328. SAR1GTPBP.

SMART

SM00177. ARF. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00231. small_GTP. 1 hit.

PROSITE

PS51417. ARF. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

312350.

SOURCE

Search...

Entry information

Entry name

ARL3_MOUSE

Accession

Primary (citable) accession number: Q9WUL7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1999
Last modified:	June 16, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)