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Protein

ADP-ribosylation factor-like protein 3

Gene

Arl3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Required for targeting proteins such as NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium (By similarity). Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms.By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi31Magnesium1
Metal bindingi48Magnesium1
Binding sitei48GTP1 Publication1
Binding sitei70GTP; via amide nitrogen1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 31GTP1 Publication8
Nucleotide bindingi67 – 71GTPBy similarity5
Nucleotide bindingi126 – 129GTP1 Publication4
Nucleotide bindingi159 – 161GTP1 Publication3

GO - Molecular functioni

  • GDP binding Source: UniProtKB
  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • microtubule binding Source: UniProtKB

GO - Biological processi

  • cilium morphogenesis Source: UniProtKB
  • cytokinesis Source: UniProtKB
  • intraciliary transport Source: MGI
  • kidney development Source: UniProtKB
  • photoreceptor cell development Source: UniProtKB
  • post-Golgi vesicle-mediated transport Source: MGI
  • small GTPase mediated signal transduction Source: UniProtKB
  • smoothened signaling pathway Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-5624138. Trafficking of myristoylated proteins to the cilium.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor-like protein 3
Gene namesi
Name:Arl3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1929699. Arl3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

Lethality by 3 weeks of age. Mice exhibit abnormal development of renal, hepatic, and pancreatic epithelial tubule structures. Mice show abnormal epithelial cell proliferation and cyst formation. Moreover, they exhibit photoreceptor degeneration as early as postnatal day 14.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi31T → N: Inhibits interaction with PDE6D. 1 Publication1
Mutagenesisi49Q → L: Does not reduce the interaction with RP2. 1 Publication1
Mutagenesisi71Q → L: Does not inhibit interaction with PDE6D. 2 Publications1
Mutagenesisi71Q → L: Inhibits RP2-dependent GTP-hydrolysis rate. 2 Publications1
Mutagenesisi98K → Q: Does not reduce the interaction with RP2. 1 Publication1
Mutagenesisi164E → A: Reduces the interaction with RP2; when associated with A-168. 1 Publication1
Mutagenesisi168D → A: Reduces the interaction with RP2; when associated with A-164. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00002074572 – 182ADP-ribosylation factor-like protein 3Add BLAST181

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineSequence analysis1
Modified residuei5PhosphoserineBy similarity1

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

EPDiQ9WUL7.
MaxQBiQ9WUL7.
PaxDbiQ9WUL7.
PRIDEiQ9WUL7.

2D gel databases

REPRODUCTION-2DPAGEIPI00124787.
Q9WUL7.

PTM databases

iPTMnetiQ9WUL7.
PhosphoSitePlusiQ9WUL7.
SwissPalmiQ9WUL7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000025035.
CleanExiMM_ARL3.
ExpressionAtlasiQ9WUL7. baseline and differential.
GenevisibleiQ9WUL7. MM.

Interactioni

Subunit structurei

Found in a complex with ARL3, RP2 and UNC119 (or UNC119B); RP2 induces hydrolysis of GTP ARL3 in the complex, leading to the release of UNC119 (or UNC119B). Interacts with SYS1. Interacts with ARL2BP; the GTP-bound form interacts with ARL2BP. Interacts with RP2; interaction is direct and stimulated with the activated GTP-bound form of ARL3. Microtubule-associated protein. Does not interact with TBCC (By similarity). Interacts with RP2 and PDE6D. Interacts with PDE6D; the interaction occurs specifically with the GTP-bound form of ARL3.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PDE6DO439244EBI-6860857,EBI-712685From a different organism.
UNC119Q134323EBI-6860857,EBI-711260From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207916. 2 interactors.
DIPiDIP-29025N.
IntActiQ9WUL7. 5 interactors.
MINTiMINT-4088340.
STRINGi10090.ENSMUSP00000026009.

Structurei

Secondary structure

1182
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 9Combined sources4
Beta strandi11 – 13Combined sources3
Helixi14 – 16Combined sources3
Beta strandi18 – 25Combined sources8
Helixi30 – 37Combined sources8
Beta strandi43 – 48Combined sources6
Beta strandi51 – 58Combined sources8
Beta strandi61 – 67Combined sources7
Helixi72 – 74Combined sources3
Helixi75 – 82Combined sources8
Beta strandi86 – 93Combined sources8
Helixi97 – 99Combined sources3
Helixi100 – 110Combined sources11
Helixi114 – 116Combined sources3
Beta strandi121 – 126Combined sources6
Beta strandi130 – 132Combined sources3
Helixi136 – 142Combined sources7
Helixi145 – 147Combined sources3
Beta strandi153 – 157Combined sources5
Turni160 – 162Combined sources3
Helixi166 – 175Combined sources10
Turni176 – 178Combined sources3
Helixi179 – 182Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FZQX-ray1.70A2-182[»]
3BH6X-ray2.60A17-177[»]
3BH7X-ray1.90A17-177[»]
4GOJX-ray2.10A/B1-182[»]
4ZI2X-ray2.20A/B1-182[»]
4ZI3X-ray2.00A/B1-182[»]
ProteinModelPortaliQ9WUL7.
SMRiQ9WUL7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WUL7.

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0074. Eukaryota.
ENOG410Y825. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiQ9WUL7.
KOiK07944.
OMAiIQEGMNW.
OrthoDBiEOG091G0O6V.
PhylomeDBiQ9WUL7.
TreeFamiTF105463.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9WUL7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLLSILRKL KSAPDQEVRI LLLGLDNAGK TTLLKQLASE DISHITPTQG
60 70 80 90 100
FNIKSVQSQG FKLNVWDIGG QRKIRPYWRS YFENTDILIY VIDSADRKRF
110 120 130 140 150
EETGQELTEL LEEEKLSCVP VLIFANKQDL LTAAPASEIA EGLNLHTIRD
160 170 180
RVWQIQSCSA LTGEGVQDGM NWVCKNVNAK KK
Length:182
Mass (Da):20,487
Last modified:November 1, 1999 - v1
Checksum:i16B85B2268ADB9DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF143241 mRNA. Translation: AAD33067.1.
BC042941 mRNA. Translation: AAH42941.1.
CCDSiCCDS38010.1.
RefSeqiNP_062692.1. NM_019718.2.
UniGeneiMm.22085.

Genome annotation databases

EnsembliENSMUST00000026009; ENSMUSP00000026009; ENSMUSG00000025035.
GeneIDi56350.
KEGGimmu:56350.
UCSCiuc008htt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF143241 mRNA. Translation: AAD33067.1.
BC042941 mRNA. Translation: AAH42941.1.
CCDSiCCDS38010.1.
RefSeqiNP_062692.1. NM_019718.2.
UniGeneiMm.22085.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FZQX-ray1.70A2-182[»]
3BH6X-ray2.60A17-177[»]
3BH7X-ray1.90A17-177[»]
4GOJX-ray2.10A/B1-182[»]
4ZI2X-ray2.20A/B1-182[»]
4ZI3X-ray2.00A/B1-182[»]
ProteinModelPortaliQ9WUL7.
SMRiQ9WUL7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207916. 2 interactors.
DIPiDIP-29025N.
IntActiQ9WUL7. 5 interactors.
MINTiMINT-4088340.
STRINGi10090.ENSMUSP00000026009.

PTM databases

iPTMnetiQ9WUL7.
PhosphoSitePlusiQ9WUL7.
SwissPalmiQ9WUL7.

2D gel databases

REPRODUCTION-2DPAGEIPI00124787.
Q9WUL7.

Proteomic databases

EPDiQ9WUL7.
MaxQBiQ9WUL7.
PaxDbiQ9WUL7.
PRIDEiQ9WUL7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026009; ENSMUSP00000026009; ENSMUSG00000025035.
GeneIDi56350.
KEGGimmu:56350.
UCSCiuc008htt.1. mouse.

Organism-specific databases

CTDi403.
MGIiMGI:1929699. Arl3.

Phylogenomic databases

eggNOGiKOG0074. Eukaryota.
ENOG410Y825. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiQ9WUL7.
KOiK07944.
OMAiIQEGMNW.
OrthoDBiEOG091G0O6V.
PhylomeDBiQ9WUL7.
TreeFamiTF105463.

Enzyme and pathway databases

ReactomeiR-MMU-5624138. Trafficking of myristoylated proteins to the cilium.

Miscellaneous databases

EvolutionaryTraceiQ9WUL7.
PROiQ9WUL7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025035.
CleanExiMM_ARL3.
ExpressionAtlasiQ9WUL7. baseline and differential.
GenevisibleiQ9WUL7. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARL3_MOUSE
AccessioniPrimary (citable) accession number: Q9WUL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: November 30, 2016
This is version 150 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.