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Protein

ADP-ribosylation factor-like protein 3

Gene

Arl3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Required for targeting proteins such as NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium (By similarity). Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms.By similarity2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi31 – 311Magnesium
Metal bindingi48 – 481Magnesium
Binding sitei48 – 481GTP1 Publication
Binding sitei70 – 701GTP; via amide nitrogen1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi24 – 318GTP1 Publication
Nucleotide bindingi67 – 715GTPBy similarity
Nucleotide bindingi126 – 1294GTP1 Publication
Nucleotide bindingi159 – 1613GTP1 Publication

GO - Molecular functioni

  • GDP binding Source: UniProtKB
  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • microtubule binding Source: UniProtKB

GO - Biological processi

  • cilium morphogenesis Source: UniProtKB
  • cytokinesis Source: UniProtKB
  • intraciliary transport Source: MGI
  • kidney development Source: UniProtKB
  • photoreceptor cell development Source: UniProtKB
  • post-Golgi vesicle-mediated transport Source: MGI
  • small GTPase mediated signal transduction Source: UniProtKB
  • smoothened signaling pathway Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-5624138. Trafficking of myristoylated proteins to the cilium.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor-like protein 3
Gene namesi
Name:Arl3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1929699. Arl3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

Lethality by 3 weeks of age. Mice exhibit abnormal development of renal, hepatic, and pancreatic epithelial tubule structures. Mice show abnormal epithelial cell proliferation and cyst formation. Moreover, they exhibit photoreceptor degeneration as early as postnatal day 14.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi31 – 311T → N: Inhibits interaction with PDE6D. 1 Publication
Mutagenesisi49 – 491Q → L: Does not reduce the interaction with RP2. 1 Publication
Mutagenesisi71 – 711Q → L: Does not inhibit interaction with PDE6D. 2 Publications
Mutagenesisi71 – 711Q → L: Inhibits RP2-dependent GTP-hydrolysis rate. 2 Publications
Mutagenesisi98 – 981K → Q: Does not reduce the interaction with RP2. 1 Publication
Mutagenesisi164 – 1641E → A: Reduces the interaction with RP2; when associated with A-168. 1 Publication
Mutagenesisi168 – 1681D → A: Reduces the interaction with RP2; when associated with A-164. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedSequence analysis
Chaini2 – 182181ADP-ribosylation factor-like protein 3PRO_0000207457Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineSequence analysis
Modified residuei5 – 51PhosphoserineBy similarity

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

EPDiQ9WUL7.
MaxQBiQ9WUL7.
PaxDbiQ9WUL7.
PRIDEiQ9WUL7.

2D gel databases

REPRODUCTION-2DPAGEIPI00124787.
Q9WUL7.

PTM databases

iPTMnetiQ9WUL7.
PhosphoSiteiQ9WUL7.
SwissPalmiQ9WUL7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000025035.
CleanExiMM_ARL3.
ExpressionAtlasiQ9WUL7. baseline and differential.
GenevisibleiQ9WUL7. MM.

Interactioni

Subunit structurei

Found in a complex with ARL3, RP2 and UNC119 (or UNC119B); RP2 induces hydrolysis of GTP ARL3 in the complex, leading to the release of UNC119 (or UNC119B). Interacts with SYS1. Interacts with ARL2BP; the GTP-bound form interacts with ARL2BP. Interacts with RP2; interaction is direct and stimulated with the activated GTP-bound form of ARL3. Microtubule-associated protein. Does not interact with TBCC (By similarity). Interacts with RP2 and PDE6D. Interacts with PDE6D; the interaction occurs specifically with the GTP-bound form of ARL3.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PDE6DO439244EBI-6860857,EBI-712685From a different organism.
UNC119Q134323EBI-6860857,EBI-711260From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207916. 2 interactions.
DIPiDIP-29025N.
IntActiQ9WUL7. 5 interactions.
MINTiMINT-4088340.
STRINGi10090.ENSMUSP00000026009.

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 94Combined sources
Beta strandi11 – 133Combined sources
Helixi14 – 163Combined sources
Beta strandi18 – 258Combined sources
Helixi30 – 378Combined sources
Beta strandi43 – 486Combined sources
Beta strandi51 – 588Combined sources
Beta strandi61 – 677Combined sources
Helixi72 – 743Combined sources
Helixi75 – 828Combined sources
Beta strandi86 – 938Combined sources
Helixi97 – 993Combined sources
Helixi100 – 11011Combined sources
Helixi114 – 1163Combined sources
Beta strandi121 – 1266Combined sources
Beta strandi130 – 1323Combined sources
Helixi136 – 1427Combined sources
Helixi145 – 1473Combined sources
Beta strandi153 – 1575Combined sources
Turni160 – 1623Combined sources
Helixi166 – 17510Combined sources
Turni176 – 1783Combined sources
Helixi179 – 1824Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FZQX-ray1.70A2-182[»]
3BH6X-ray2.60A17-177[»]
3BH7X-ray1.90A17-177[»]
4GOJX-ray2.10A/B1-182[»]
4ZI2X-ray2.20A/B1-182[»]
4ZI3X-ray2.00A/B1-182[»]
ProteinModelPortaliQ9WUL7.
SMRiQ9WUL7. Positions 2-177.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WUL7.

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0074. Eukaryota.
ENOG410Y825. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiQ9WUL7.
KOiK07944.
OMAiIQEGMNW.
OrthoDBiEOG091G0O6V.
PhylomeDBiQ9WUL7.
TreeFamiTF105463.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9WUL7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLLSILRKL KSAPDQEVRI LLLGLDNAGK TTLLKQLASE DISHITPTQG
60 70 80 90 100
FNIKSVQSQG FKLNVWDIGG QRKIRPYWRS YFENTDILIY VIDSADRKRF
110 120 130 140 150
EETGQELTEL LEEEKLSCVP VLIFANKQDL LTAAPASEIA EGLNLHTIRD
160 170 180
RVWQIQSCSA LTGEGVQDGM NWVCKNVNAK KK
Length:182
Mass (Da):20,487
Last modified:November 1, 1999 - v1
Checksum:i16B85B2268ADB9DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF143241 mRNA. Translation: AAD33067.1.
BC042941 mRNA. Translation: AAH42941.1.
CCDSiCCDS38010.1.
RefSeqiNP_062692.1. NM_019718.2.
UniGeneiMm.22085.

Genome annotation databases

EnsembliENSMUST00000026009; ENSMUSP00000026009; ENSMUSG00000025035.
GeneIDi56350.
KEGGimmu:56350.
UCSCiuc008htt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF143241 mRNA. Translation: AAD33067.1.
BC042941 mRNA. Translation: AAH42941.1.
CCDSiCCDS38010.1.
RefSeqiNP_062692.1. NM_019718.2.
UniGeneiMm.22085.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FZQX-ray1.70A2-182[»]
3BH6X-ray2.60A17-177[»]
3BH7X-ray1.90A17-177[»]
4GOJX-ray2.10A/B1-182[»]
4ZI2X-ray2.20A/B1-182[»]
4ZI3X-ray2.00A/B1-182[»]
ProteinModelPortaliQ9WUL7.
SMRiQ9WUL7. Positions 2-177.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207916. 2 interactions.
DIPiDIP-29025N.
IntActiQ9WUL7. 5 interactions.
MINTiMINT-4088340.
STRINGi10090.ENSMUSP00000026009.

PTM databases

iPTMnetiQ9WUL7.
PhosphoSiteiQ9WUL7.
SwissPalmiQ9WUL7.

2D gel databases

REPRODUCTION-2DPAGEIPI00124787.
Q9WUL7.

Proteomic databases

EPDiQ9WUL7.
MaxQBiQ9WUL7.
PaxDbiQ9WUL7.
PRIDEiQ9WUL7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026009; ENSMUSP00000026009; ENSMUSG00000025035.
GeneIDi56350.
KEGGimmu:56350.
UCSCiuc008htt.1. mouse.

Organism-specific databases

CTDi403.
MGIiMGI:1929699. Arl3.

Phylogenomic databases

eggNOGiKOG0074. Eukaryota.
ENOG410Y825. LUCA.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiQ9WUL7.
KOiK07944.
OMAiIQEGMNW.
OrthoDBiEOG091G0O6V.
PhylomeDBiQ9WUL7.
TreeFamiTF105463.

Enzyme and pathway databases

ReactomeiR-MMU-5624138. Trafficking of myristoylated proteins to the cilium.

Miscellaneous databases

EvolutionaryTraceiQ9WUL7.
PROiQ9WUL7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025035.
CleanExiMM_ARL3.
ExpressionAtlasiQ9WUL7. baseline and differential.
GenevisibleiQ9WUL7. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARL3_MOUSE
AccessioniPrimary (citable) accession number: Q9WUL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: September 7, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.