Reviewed, UniProtKB/Swiss-Prot Q9WUL0 (TOP1_RAT)

Names and origin

Protein names

Recommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I

Gene names

Name:

Top1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	767 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subunit structure	Monomer By similarity.
Subcellular location	Nucleus › nucleolus. Nucleus › nucleoplasm. Note: Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli By similarity.
Post-translational modification	Sumoylated. Lys-119 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment By similarity.
Miscellaneous	Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils. When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Sequence similarities	Belongs to the eukaryotic type I topoisomerase family.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
PTM	Acetylation Isopeptide bond Ubl conjugation
Gene Ontology (GO)
Biological process	DNA topological change Inferred from direct assay. Source: RGD DNA unwinding during replication Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell nucleoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro DNA topoisomerase type I activity Inferred from direct assay. Source: RGD chromatin DNA binding Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 767	766	DNA topoisomerase 1		PRO_0000145203
Regions
	Compositional bias	23 – 206	184	Lys-rich
Sites
	Active site	725	1	O-(3'-phospho-DNA)-tyrosine intermediate By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	282	1	N6-acetyllysine By similarity
	Cross-link	105		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) Probable
	Cross-link	119		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
	Cross-link	155		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) Probable

Sequences

	Sequence		Length	Mass (Da)
	Q9WUL0-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 79103F06DA61D73D	FASTA	767	90,760
	10 20 30 40 50 60 MSGDHLHNDS QIEADFRLND SHKHKDKHKD REHRHKEHKK DKDKDREKSK HSNSEHKDSE 70 80 90 100 110 120 KKHKEKEKTK HKDGSSDKHK DKHKDRDKEK RKEEKIRAAG DAKIKKEKEN GFSSPPRIKD 130 140 150 160 170 180 EPEDDGYFAP PKEDIKPLKR PRDEDDADYK PKKIKTEDIK KEKKRKLEEE EDGKLKKPKN 190 200 210 220 230 240 KDKDKKVAEP DNKKKKAKKE EEQKWKWWEE ERYPEGIKWK FLEHKGPVFA PPYEPLPEGV 250 260 270 280 290 300 KFYYDGKVMK LSPKAEEVAT FFAKMLDHEY TTKEIFRKNF FKDWRKEMTN DEKNTITNLS 310 320 330 340 350 360 KCDFTQMSQY FKAQSEARKQ MSKEEKLKIK EENEKLLKEY GFCVMDNHRE RIANFKIEPP 370 380 390 400 410 420 GLFRGRGNHP KMGMLKRRIM PEDIIINCSK DAKVPSPPPG HKWKEVRHDN KVTWLVSWTE 430 440 450 460 470 480 NIQGSIKYIM LNPSSRIKGE KDWQKYETAR RLKKCVDKIR NQYREDWKSK EMKVRQRAVA 490 500 510 520 530 540 LYFIDKLALR AGNEKEEGET ADTVGCCSLR VEHINLHPEL DGQEYVVEFD FPGKDSIRYY 550 560 570 580 590 600 NKVPVEKRVF KNLQLFMENK QPEDDLFDRL NTGILNKHLQ DLMEGLTAKV FRTYNASITL 610 620 630 640 650 660 QQQLKELTAP DENVPAKILS YNRANRAVAI LCNHQRAPPK TFEKSMMNLQ SKIDAKKDQL 670 680 690 700 710 720 ADARKDLKSA KADAKVMKDA KTKKVVESKK KAVQRLEEQL MKLEVQATDR EENKQIALGT 730 740 750 760 SKLNYLDPRI TVAWCKKWGV PIEKIYNKTQ REKFAWAIDM TDEDYEF

References

[1]	"Sequencing of rat topoisomerase I between sensitive and camptothecin-resistant C6 glioblastoma cell lines." Pourquier P., Gioffre C., Ueng L.-M., Robert J., Pommier Y. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Glioblastoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF140782 mRNA. Translation: AAD30137.1.
IPI	IPI00204133.
RefSeq	NP_072137.1.
UniGene	Rn.91572
3D structure databases
HSSP	HSSP built from PDB template 1EJ9 based on UniProtKB P11387.
SMR	Q9WUL0. Positions 203-767.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9WUL0.
PTM databases
PhosphoSite	Q9WUL0.
Proteomic databases
PRIDE	Q9WUL0.
Genome annotation databases
Ensembl	ENSRNOT00000021691; ENSRNOP00000021691; ENSRNOG00000030310; Rattus norvegicus. [Genome view] ENSRNOT00000034781; ENSRNOP00000034639; ENSRNOG00000030310; Rattus norvegicus. [Genome view]
GeneID	64550.
KEGG	rno:64550.
Organism-specific databases
CTD	64550.
RGD	621246. Top1.
Phylogenomic databases
HOVERGEN	Q9WUL0.
Enzyme and pathway databases
BRENDA	5.99.1.2. 248.
Gene expression databases
Genevestigator	Q9WUL0.
GermOnline	ENSRNOG00000030310. Rattus norvegicus.
Family and domain databases
InterPro	IPR018521. TopoI_AS. IPR001631. TopoI_C. IPR013499. TopoI_C_euk. IPR014711. TopoI_cat_a-hlx-sub_euk. IPR014727. TopoI_cat_a/b-sub_euk. IPR013500. TopoI_cat_euk. IPR013030. TopoI_DNA-bd_mixed-a/b_euk. IPR008336. TopoI_DNA_bd_euk. [Graphical view]
Gene3D	G3DSA:3.90.15.10. TopoI_cat_a-hlx-sub_euk. 1 hit. G3DSA:1.10.132.10. TopoI_cat_a/b-sub_euk. 1 hit. G3DSA:2.170.11.10. TopoI_DNA-bd_mixed-a/b_euk. 1 hit.
Pfam	PF01028. Topoisom_I. 1 hit. PF02919. Topoisom_I_N. 1 hit. [Graphical view]
PRINTS	PR00416. EUTPISMRASEI.
SMART	SM00435. TOPEUc. 1 hit. [Graphical view]
PROSITE	PS00176. TOPOISOMERASE_I_EUK. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	613440.

Entry information

Entry name

TOP1_RAT

Accession

Primary (citable) accession number: Q9WUL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	November 1, 1999
Last modified:	October 13, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)