Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9WUK6

- ZBT18_MOUSE

UniProt

Q9WUK6 - ZBT18_MOUSE

Protein

Zinc finger and BTB domain-containing protein 18

Gene

Zbtb18

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Transcriptional repressor that plays a role in various developmental processes such as myogenesis and brain development. Specifically binds the consensus DNA sequence 5'-[AC]ACATCTG[GT][AC]-3' which contains the E box core, and acts by recruiting chromatin remodeling multiprotein complexes. Plays a key role in myogenesis by directly repressing the expression of ID2 and ID3, 2 inhibitors of skeletal myogenesis. Also involved in controlling cell division of progenitor cells and regulating the survival of postmitotic cortical neurons. May also play a role in the organization of chromosomes in the nucleus.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri370 – 39223C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri410 – 43223C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri438 – 46023C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri466 – 48924C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. sequence-specific DNA binding Source: UniProtKB

    GO - Biological processi

    1. cerebellum development Source: MGI
    2. cerebral cortex development Source: MGI
    3. hippocampus development Source: MGI
    4. homeostasis of number of cells Source: MGI
    5. in utero embryonic development Source: MGI
    6. negative regulation of transcription, DNA-templated Source: UniProtKB
    7. negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    8. neuron development Source: MGI
    9. regulation of cell division Source: MGI
    10. skeletal muscle tissue development Source: UniProtKB
    11. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Zinc finger and BTB domain-containing protein 18
    Alternative name(s):
    58 kDa repressor protein
    Transcriptional repressor RP58
    Zinc finger protein 238
    Short name:
    Zfp-238
    Gene namesi
    Name:Zbtb18
    Synonyms:Rp58, Zfp238, Znf238
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:1353609. Zbtb18.

    Subcellular locationi

    Nucleus
    Note: Associates with condensed chromatin.

    GO - Cellular componenti

    1. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Death shortly after birth. Brain of mutant mice display dysplasia of the neocortex and of the hippocampus, reduction of the number of mature cortical neurons and defects of laminar organization. In the cortex, an impaired cell-division patterning during the late embryonic stage and an enhancement of apoptosis of the postmitotic neurons are observed.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 522522Zinc finger and BTB domain-containing protein 18PRO_0000047478Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei516 – 5161PhosphoserineBy similarity
    Modified residuei517 – 5171PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9WUK6.
    PRIDEiQ9WUK6.

    PTM databases

    PhosphoSiteiQ9WUK6.

    Expressioni

    Developmental stagei

    In the developing brain, expression is detected at embryonic day (E) 10 in the neuroepithelium, and subsequently in the ventricular zones of the cerebral cortex in the E12 embryo. Strong expression is observed in the preplate in the cerebral cortex from this stage onward. High levels of expression continue to be detected in the cortical plate and subventricular zone of the neocortex, hippocampus, and parts of the amygdala, but not in the thalamus or striatum. In the developing cerebral cortex, it is expressed both in postmitotic glutamatergic projection neurons and in their progenitor cells, but not in GABAergic interneurons. Also expressed in the adult neocortex, hippocampus, parts of the amygdala and granule cells in the cerebellum.1 Publication

    Gene expression databases

    ArrayExpressiQ9WUK6.
    BgeeiQ9WUK6.
    CleanExiMM_ZFP238.
    GenevestigatoriQ9WUK6.

    Interactioni

    Subunit structurei

    Interacts with DNMT3A.By similarity

    Protein-protein interaction databases

    BioGridi206006. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9WUK6.
    SMRiQ9WUK6. Positions 2-113, 370-520.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini24 – 9168BTBPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni310 – 427118Interaction with DNMT3ABy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
    Contains 4 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri370 – 39223C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri410 – 43223C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri438 – 46023C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri466 – 48924C2H2-type 4PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5048.
    GeneTreeiENSGT00750000117353.
    HOGENOMiHOG000234147.
    HOVERGENiHBG059113.
    InParanoidiQ80YQ4.
    PhylomeDBiQ9WUK6.

    Family and domain databases

    Gene3Di3.30.160.60. 3 hits.
    3.30.710.10. 1 hit.
    InterProiIPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR013069. BTB_POZ.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    PfamiPF00651. BTB. 1 hit.
    PF00096. zf-C2H2. 1 hit.
    [Graphical view]
    SMARTiSM00225. BTB. 1 hit.
    SM00355. ZnF_C2H2. 4 hits.
    [Graphical view]
    SUPFAMiSSF54695. SSF54695. 1 hit.
    PROSITEiPS50097. BTB. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 4 hits.
    PS50157. ZINC_FINGER_C2H2_2. 4 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9WUK6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEFPDHSRHL LQCLSEQRHQ GFLCDCTVLV GDAQFRAHRA VLASCSMYFH    50
    LFYKDQLDKR DIVHLNSDIV TAPAFALLLE FMYEGKLQFK DLPIEDVLAA 100
    ASYLHMYDIV KVCKKKLKEK ATTEADSTKK EEDASSCSDK VESLSDGSSH 150
    MAGDLPSDED EGEDDKLNIL PSKRDLAAEP GNMWMRLPSD SAGIPQAGGE 200
    AEPHATAAGK TVASPCSSTE SLSQRSVTSV RDSADVDCVL DLSVKSSLSG 250
    VENLNSSYFS SQDVLRSNLV QVKVEKEASC DESDVGTNDY DMEHSTVKES 300
    VSTNNRVQYE PAHLAPLRED SVLRELDRED KASDDEMMTP ESERVQVEGG 350
    MENSLLPYVS NILSPAGQIF MCPLCNKVFP SPHILQIHLS THFREQDGIR 400
    SKPAADVNVP TCSLCGKTFS CMYTLKRHER THSGEKPYTC TQCGKSFQYS 450
    HNLSRHAVVH TREKPHACKW CERRFTQSGD LYRHIRKFHC ELVNSLSVKS 500
    EALSLPTVRD WTLEDSSQEL WK 522
    Length:522
    Mass (Da):58,385
    Last modified:November 1, 1999 - v1
    Checksum:i41E169E690D8B849
    GO

    Sequence cautioni

    The sequence AAH50909.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti42 – 421L → V in AAH54742. (PubMed:15489334)Curated
    Sequence conflicti42 – 421L → V in AAH54529. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF140224 mRNA. Translation: AAD34547.1.
    AK147200 mRNA. Translation: BAE27758.1.
    BC050909 mRNA. Translation: AAH50909.1. Different initiation.
    BC054529 mRNA. Translation: AAH54529.1.
    BC054742 mRNA. Translation: AAH54742.1.
    CCDSiCCDS15553.1.
    RefSeqiNP_001012330.1. NM_001012330.1.
    NP_038943.3. NM_013915.3.
    XP_006496956.1. XM_006496893.1.
    XP_006496957.1. XM_006496894.1.
    XP_006496958.1. XM_006496895.1.
    XP_006496959.1. XM_006496896.1.
    UniGeneiMm.330700.

    Genome annotation databases

    EnsembliENSMUST00000077225; ENSMUSP00000076463; ENSMUSG00000063659.
    GeneIDi30928.
    KEGGimmu:30928.
    UCSCiuc007dul.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF140224 mRNA. Translation: AAD34547.1 .
    AK147200 mRNA. Translation: BAE27758.1 .
    BC050909 mRNA. Translation: AAH50909.1 . Different initiation.
    BC054529 mRNA. Translation: AAH54529.1 .
    BC054742 mRNA. Translation: AAH54742.1 .
    CCDSi CCDS15553.1.
    RefSeqi NP_001012330.1. NM_001012330.1.
    NP_038943.3. NM_013915.3.
    XP_006496956.1. XM_006496893.1.
    XP_006496957.1. XM_006496894.1.
    XP_006496958.1. XM_006496895.1.
    XP_006496959.1. XM_006496896.1.
    UniGenei Mm.330700.

    3D structure databases

    ProteinModelPortali Q9WUK6.
    SMRi Q9WUK6. Positions 2-113, 370-520.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 206006. 1 interaction.

    PTM databases

    PhosphoSitei Q9WUK6.

    Proteomic databases

    PaxDbi Q9WUK6.
    PRIDEi Q9WUK6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000077225 ; ENSMUSP00000076463 ; ENSMUSG00000063659 .
    GeneIDi 30928.
    KEGGi mmu:30928.
    UCSCi uc007dul.2. mouse.

    Organism-specific databases

    CTDi 10472.
    MGIi MGI:1353609. Zbtb18.

    Phylogenomic databases

    eggNOGi COG5048.
    GeneTreei ENSGT00750000117353.
    HOGENOMi HOG000234147.
    HOVERGENi HBG059113.
    InParanoidi Q80YQ4.
    PhylomeDBi Q9WUK6.

    Miscellaneous databases

    NextBioi 307332.
    PROi Q9WUK6.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9WUK6.
    Bgeei Q9WUK6.
    CleanExi MM_ZFP238.
    Genevestigatori Q9WUK6.

    Family and domain databases

    Gene3Di 3.30.160.60. 3 hits.
    3.30.710.10. 1 hit.
    InterProi IPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR013069. BTB_POZ.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    Pfami PF00651. BTB. 1 hit.
    PF00096. zf-C2H2. 1 hit.
    [Graphical view ]
    SMARTi SM00225. BTB. 1 hit.
    SM00355. ZnF_C2H2. 4 hits.
    [Graphical view ]
    SUPFAMi SSF54695. SSF54695. 1 hit.
    PROSITEi PS50097. BTB. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 4 hits.
    PS50157. ZINC_FINGER_C2H2_2. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of the gene encoding RP58, a sequence-specific transrepressor associated with heterochromatin."
      Meng G., Inazawa J., Ishida R., Tokura K., Nakahara K., Aoki K., Kasai M.
      Gene 242:59-64(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver and Thymus.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Brain.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    4. "Spatial and temporal expression of RP58, a novel zinc finger transcriptional repressor, in mouse brain."
      Ohtaka-Maruyama C., Miwa A., Kawano H., Kasai M., Okado H.
      J. Comp. Neurol. 502:1098-1108(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    5. "The transcriptional repressor RP58 is crucial for cell-division patterning and neuronal survival in the developing cortex."
      Okado H., Ohtaka-Maruyama C., Sugitani Y., Fukuda Y., Ishida R., Hirai S., Miwa A., Takahashi A., Aoki K., Mochida K., Suzuki O., Honda T., Nakajima K., Ogawa M., Terashima T., Matsuda J., Kawano H., Kasai M.
      Dev. Biol. 331:140-151(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
    6. Cited for: FUNCTION.

    Entry informationi

    Entry nameiZBT18_MOUSE
    AccessioniPrimary (citable) accession number: Q9WUK6
    Secondary accession number(s): Q3UHU9, Q7TMA6, Q80YQ4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2002
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3