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Q9WUK6 (ZBT18_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger and BTB domain-containing protein 18
Alternative name(s):
58 kDa repressor protein
Transcriptional repressor RP58
Zinc finger protein 238
Short name=Zfp-238
Gene names
Name:Zbtb18
Synonyms:Rp58, Zfp238, Znf238
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length522 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcriptional repressor that plays a role in various developmental processes such as myogenesis and brain development. Specifically binds the consensus DNA sequence 5'-[AC]ACATCTG[GT][AC]-3' which contains the E box core, and acts by recruiting chromatin remodeling multiprotein complexes. Plays a key role in myogenesis by directly repressing the expression of ID2 and ID3, 2 inhibitors of skeletal myogenesis. Also involved in controlling cell division of progenitor cells and regulating the survival of postmitotic cortical neurons. May also play a role in the organization of chromosomes in the nucleus. Ref.5 Ref.6

Subunit structure

Interacts with DNMT3A By similarity.

Subcellular location

Nucleus. Note: Associates with condensed chromatin.

Developmental stage

In the developing brain, expression is detected at embryonic day (E) 10 in the neuroepithelium, and subsequently in the ventricular zones of the cerebral cortex in the E12 embryo. Strong expression is observed in the preplate in the cerebral cortex from this stage onward. High levels of expression continue to be detected in the cortical plate and subventricular zone of the neocortex, hippocampus, and parts of the amygdala, but not in the thalamus or striatum. In the developing cerebral cortex, it is expressed both in postmitotic glutamatergic projection neurons and in their progenitor cells, but not in GABAergic interneurons. Also expressed in the adult neocortex, hippocampus, parts of the amygdala and granule cells in the cerebellum. Ref.5

Disruption phenotype

Death shortly after birth. Brain of mutant mice display dysplasia of the neocortex and of the hippocampus, reduction of the number of mature cortical neurons and defects of laminar organization. In the cortex, an impaired cell-division patterning during the late embryonic stage and an enhancement of apoptosis of the postmitotic neurons are observed. Ref.5

Sequence similarities

Belongs to the krueppel C2H2-type zinc-finger protein family. ZBTB18 subfamily.

Contains 1 BTB (POZ) domain.

Contains 4 C2H2-type zinc fingers.

Sequence caution

The sequence AAH50909.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionDevelopmental protein
Repressor
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcerebellum development

Inferred from mutant phenotype Ref.5. Source: MGI

cerebral cortex development

Inferred from mutant phenotype Ref.5. Source: MGI

hippocampus development

Inferred from mutant phenotype Ref.5. Source: MGI

homeostasis of number of cells

Inferred from mutant phenotype Ref.5. Source: MGI

in utero embryonic development

Inferred from mutant phenotype Ref.5. Source: MGI

negative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay Ref.6. Source: UniProtKB

negative regulation of transcription, DNA-templated

Inferred from direct assay Ref.6. Source: UniProtKB

neuron development

Inferred from mutant phenotype Ref.5. Source: MGI

regulation of cell division

Inferred from mutant phenotype Ref.5. Source: MGI

skeletal muscle tissue development

Inferred from mutant phenotype Ref.6. Source: UniProtKB

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from direct assay PubMed 18262495Ref.5. Source: MGI

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

sequence-specific DNA binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 522522Zinc finger and BTB domain-containing protein 18
PRO_0000047478

Regions

Domain24 – 9168BTB
Zinc finger370 – 39223C2H2-type 1
Zinc finger410 – 43223C2H2-type 2
Zinc finger438 – 46023C2H2-type 3
Zinc finger466 – 48924C2H2-type 4
Region310 – 427118Interaction with DNMT3A By similarity

Amino acid modifications

Modified residue5161Phosphoserine By similarity
Modified residue5171Phosphoserine By similarity

Experimental info

Sequence conflict421L → V in AAH54742. Ref.3
Sequence conflict421L → V in AAH54529. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9WUK6 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 41E169E690D8B849

FASTA52258,385
        10         20         30         40         50         60 
MEFPDHSRHL LQCLSEQRHQ GFLCDCTVLV GDAQFRAHRA VLASCSMYFH LFYKDQLDKR 

        70         80         90        100        110        120 
DIVHLNSDIV TAPAFALLLE FMYEGKLQFK DLPIEDVLAA ASYLHMYDIV KVCKKKLKEK 

       130        140        150        160        170        180 
ATTEADSTKK EEDASSCSDK VESLSDGSSH MAGDLPSDED EGEDDKLNIL PSKRDLAAEP 

       190        200        210        220        230        240 
GNMWMRLPSD SAGIPQAGGE AEPHATAAGK TVASPCSSTE SLSQRSVTSV RDSADVDCVL 

       250        260        270        280        290        300 
DLSVKSSLSG VENLNSSYFS SQDVLRSNLV QVKVEKEASC DESDVGTNDY DMEHSTVKES 

       310        320        330        340        350        360 
VSTNNRVQYE PAHLAPLRED SVLRELDRED KASDDEMMTP ESERVQVEGG MENSLLPYVS 

       370        380        390        400        410        420 
NILSPAGQIF MCPLCNKVFP SPHILQIHLS THFREQDGIR SKPAADVNVP TCSLCGKTFS 

       430        440        450        460        470        480 
CMYTLKRHER THSGEKPYTC TQCGKSFQYS HNLSRHAVVH TREKPHACKW CERRFTQSGD 

       490        500        510        520 
LYRHIRKFHC ELVNSLSVKS EALSLPTVRD WTLEDSSQEL WK 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of the gene encoding RP58, a sequence-specific transrepressor associated with heterochromatin."
Meng G., Inazawa J., Ishida R., Tokura K., Nakahara K., Aoki K., Kasai M.
Gene 242:59-64(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver and Thymus.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Brain.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[4]"Spatial and temporal expression of RP58, a novel zinc finger transcriptional repressor, in mouse brain."
Ohtaka-Maruyama C., Miwa A., Kawano H., Kasai M., Okado H.
J. Comp. Neurol. 502:1098-1108(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[5]"The transcriptional repressor RP58 is crucial for cell-division patterning and neuronal survival in the developing cortex."
Okado H., Ohtaka-Maruyama C., Sugitani Y., Fukuda Y., Ishida R., Hirai S., Miwa A., Takahashi A., Aoki K., Mochida K., Suzuki O., Honda T., Nakajima K., Ogawa M., Terashima T., Matsuda J., Kawano H., Kasai M.
Dev. Biol. 331:140-151(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
[6]"A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58."
Yokoyama S., Ito Y., Ueno-Kudoh H., Shimizu H., Uchibe K., Albini S., Mitsuoka K., Miyaki S., Kiso M., Nagai A., Hikata T., Osada T., Fukuda N., Yamashita S., Harada D., Mezzano V., Kasai M., Puri P.L. expand/collapse author list , Hayashizaki Y., Okado H., Hashimoto M., Asahara H.
Dev. Cell 17:836-848(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF140224 mRNA. Translation: AAD34547.1.
AK147200 mRNA. Translation: BAE27758.1.
BC050909 mRNA. Translation: AAH50909.1. Different initiation.
BC054529 mRNA. Translation: AAH54529.1.
BC054742 mRNA. Translation: AAH54742.1.
CCDSCCDS15553.1.
RefSeqNP_001012330.1. NM_001012330.1.
NP_038943.3. NM_013915.3.
XP_006496956.1. XM_006496893.1.
XP_006496957.1. XM_006496894.1.
XP_006496958.1. XM_006496895.1.
XP_006496959.1. XM_006496896.1.
UniGeneMm.330700.

3D structure databases

ProteinModelPortalQ9WUK6.
SMRQ9WUK6. Positions 2-113, 370-520.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid206006. 1 interaction.

PTM databases

PhosphoSiteQ9WUK6.

Proteomic databases

PaxDbQ9WUK6.
PRIDEQ9WUK6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000077225; ENSMUSP00000076463; ENSMUSG00000063659.
GeneID30928.
KEGGmmu:30928.
UCSCuc007dul.2. mouse.

Organism-specific databases

CTD10472.
MGIMGI:1353609. Zbtb18.

Phylogenomic databases

eggNOGCOG5048.
GeneTreeENSGT00750000117353.
HOGENOMHOG000234147.
HOVERGENHBG059113.
InParanoidQ80YQ4.
PhylomeDBQ9WUK6.

Gene expression databases

ArrayExpressQ9WUK6.
BgeeQ9WUK6.
CleanExMM_ZFP238.
GenevestigatorQ9WUK6.

Family and domain databases

Gene3D3.30.160.60. 3 hits.
3.30.710.10. 1 hit.
InterProIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF00651. BTB. 1 hit.
PF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 4 hits.
[Graphical view]
SUPFAMSSF54695. SSF54695. 1 hit.
PROSITEPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio307332.
PROQ9WUK6.
SOURCESearch...

Entry information

Entry nameZBT18_MOUSE
AccessionPrimary (citable) accession number: Q9WUK6
Secondary accession number(s): Q3UHU9, Q7TMA6, Q80YQ4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2002
Last sequence update: November 1, 1999
Last modified: July 9, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot