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Protein

Eukaryotic translation initiation factor 4H

Gene

Eif4h

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Stimulates the RNA helicase activity of EIF4A in the translation initiation complex. Binds weakly mRNA (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • cytoplasmic translation Source: GO_Central
  • developmental growth Source: MGI
  • eukaryotic translation initiation factor 4F complex assembly Source: GO_Central
  • formation of translation preinitiation complex Source: GO_Central
  • sexual reproduction Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-MMU-72649. Translation initiation complex formation.
R-MMU-72662. Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S.
R-MMU-72702. Ribosomal scanning and start codon recognition.
R-MMU-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 4H
Short name:
eIF-4H
Alternative name(s):
Williams-Beuren syndrome chromosomal region 1 protein homolog
Gene namesi
Name:Eif4h
Synonyms:Wbscr1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1341822. Eif4h.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000816202 – 248Eukaryotic translation initiation factor 4HAdd BLAST247

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei13PhosphoserineBy similarity1
Modified residuei14PhosphoserineBy similarity1
Modified residuei19Omega-N-methylarginineCombined sources1
Modified residuei21PhosphoserineBy similarity1
Modified residuei22Omega-N-methylarginineCombined sources1
Modified residuei24PhosphoserineBy similarity1
Modified residuei32PhosphoserineBy similarity1
Modified residuei136Omega-N-methylarginineCombined sources1
Modified residuei166Omega-N-methylarginineCombined sources1
Modified residuei175Omega-N-methylarginineCombined sources1
Modified residuei230PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ9WUK2.
PaxDbiQ9WUK2.
PRIDEiQ9WUK2.

PTM databases

iPTMnetiQ9WUK2.
PhosphoSitePlusiQ9WUK2.
SwissPalmiQ9WUK2.

Miscellaneous databases

PMAP-CutDBQ9WUK2.

Expressioni

Tissue specificityi

Expressed at high levels in heart, liver and testis and at lower levels in brain, spleen, lung, skeletal muscle, kidney and embryonic tissues. Both isoforms are expressed at similar levels.1 Publication

Gene expression databases

BgeeiENSMUSG00000040731.
CleanExiMM_EIF4H.
ExpressionAtlasiQ9WUK2. baseline and differential.
GenevisibleiQ9WUK2. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204551. 1 interactor.
MINTiMINT-1869870.
STRINGi10090.ENSMUSP00000048833.

Structurei

Secondary structure

1248
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi38 – 40Combined sources3
Beta strandi42 – 48Combined sources7
Helixi55 – 61Combined sources7
Turni62 – 64Combined sources3
Beta strandi67 – 74Combined sources8
Beta strandi76 – 78Combined sources3
Beta strandi80 – 91Combined sources12
Helixi92 – 98Combined sources7
Helixi99 – 101Combined sources3
Beta strandi112 – 115Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DNGNMR-A34-123[»]
ProteinModelPortaliQ9WUK2.
SMRiQ9WUK2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WUK2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini42 – 118RRMPROSITE-ProRule annotationAdd BLAST77

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0118. Eukaryota.
COG0724. LUCA.
GeneTreeiENSGT00530000063406.
HOGENOMiHOG000013063.
HOVERGENiHBG018193.
InParanoidiQ9WUK2.
OMAiPRTVSEP.
OrthoDBiEOG091G0PIF.
PhylomeDBiQ9WUK2.
TreeFamiTF313897.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: Q9WUK2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADFDTYDDR AYSSFGGGRG SRGSAGGHGS RSQKELPTEP PYTAYVGNLP
60 70 80 90 100
FNTVQGDIDA IFKDLSIRSV RLVRDKDTDK FKGFCYVEFD EVDSLKEALT
110 120 130 140 150
YDGALLGDRS LRVDIAEGRK QDKGGFGFRK GGPDDRGMGG SRESRGGWDS
160 170 180 190 200
RDDFNSGYRD DFLGGRGGSR PGDRRAGPPM GSRFRDGPPL RGSNMDFREP
210 220 230 240
TEEERAQRPR LQLKPRTVAT PLNQVANPNS AIFGGARPRE EVVQKEQE
Length:248
Mass (Da):27,341
Last modified:January 23, 2007 - v3
Checksum:i23E5393BAF183477
GO
Isoform Short (identifier: Q9WUK2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     137-156: Missing.

Show »
Length:228
Mass (Da):25,186
Checksum:iA8F5B0957AA856E9
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005800137 – 156Missing in isoform Short. 1 PublicationAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF139987 Genomic DNA. Translation: AAD34859.1.
AF139987 Genomic DNA. Translation: AAD34860.1.
AF289664 Genomic DNA. Translation: AAF99330.1.
BC014796 mRNA. Translation: AAH14796.1.
CCDSiCCDS19723.1. [Q9WUK2-1]
RefSeqiNP_001299796.1. NM_001312867.1. [Q9WUK2-2]
NP_291039.1. NM_033561.2. [Q9WUK2-1]
UniGeneiMm.27955.
Mm.351377.

Genome annotation databases

EnsembliENSMUST00000036125; ENSMUSP00000048833; ENSMUSG00000040731. [Q9WUK2-2]
ENSMUST00000202622; ENSMUSP00000143910; ENSMUSG00000040731. [Q9WUK2-1]
GeneIDi22384.
KEGGimmu:22384.
UCSCiuc008zwp.1. mouse. [Q9WUK2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF139987 Genomic DNA. Translation: AAD34859.1.
AF139987 Genomic DNA. Translation: AAD34860.1.
AF289664 Genomic DNA. Translation: AAF99330.1.
BC014796 mRNA. Translation: AAH14796.1.
CCDSiCCDS19723.1. [Q9WUK2-1]
RefSeqiNP_001299796.1. NM_001312867.1. [Q9WUK2-2]
NP_291039.1. NM_033561.2. [Q9WUK2-1]
UniGeneiMm.27955.
Mm.351377.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DNGNMR-A34-123[»]
ProteinModelPortaliQ9WUK2.
SMRiQ9WUK2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204551. 1 interactor.
MINTiMINT-1869870.
STRINGi10090.ENSMUSP00000048833.

PTM databases

iPTMnetiQ9WUK2.
PhosphoSitePlusiQ9WUK2.
SwissPalmiQ9WUK2.

Proteomic databases

EPDiQ9WUK2.
PaxDbiQ9WUK2.
PRIDEiQ9WUK2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036125; ENSMUSP00000048833; ENSMUSG00000040731. [Q9WUK2-2]
ENSMUST00000202622; ENSMUSP00000143910; ENSMUSG00000040731. [Q9WUK2-1]
GeneIDi22384.
KEGGimmu:22384.
UCSCiuc008zwp.1. mouse. [Q9WUK2-1]

Organism-specific databases

CTDi7458.
MGIiMGI:1341822. Eif4h.

Phylogenomic databases

eggNOGiKOG0118. Eukaryota.
COG0724. LUCA.
GeneTreeiENSGT00530000063406.
HOGENOMiHOG000013063.
HOVERGENiHBG018193.
InParanoidiQ9WUK2.
OMAiPRTVSEP.
OrthoDBiEOG091G0PIF.
PhylomeDBiQ9WUK2.
TreeFamiTF313897.

Enzyme and pathway databases

ReactomeiR-MMU-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-MMU-72649. Translation initiation complex formation.
R-MMU-72662. Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S.
R-MMU-72702. Ribosomal scanning and start codon recognition.
R-MMU-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.

Miscellaneous databases

ChiTaRSiEif4h. mouse.
EvolutionaryTraceiQ9WUK2.
PMAP-CutDBQ9WUK2.
PROiQ9WUK2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040731.
CleanExiMM_EIF4H.
ExpressionAtlasiQ9WUK2. baseline and differential.
GenevisibleiQ9WUK2. MM.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF4H_MOUSE
AccessioniPrimary (citable) accession number: Q9WUK2
Secondary accession number(s): Q9WUK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.