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Protein

Insulin-like 3

Gene

Insl3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Seems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor (By similarity).By similarity

GO - Molecular functioni

  • G-protein coupled receptor binding Source: RGD

GO - Biological processi

  • adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Source: RGD
  • gonad development Source: RGD
  • male gonad development Source: RGD
  • negative regulation of apoptotic process Source: RGD
  • negative regulation of cell proliferation Source: RGD
  • oocyte maturation Source: RGD
  • positive regulation of cAMP biosynthetic process Source: RGD
  • positive regulation of cell proliferation Source: RGD
  • positive regulation of epithelial cell migration Source: Ensembl
  • positive regulation of wound healing Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hormone

Enzyme and pathway databases

ReactomeiR-RNO-418555. G alpha (s) signalling events.
R-RNO-444821. Relaxin receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin-like 3
Alternative name(s):
Leydig insulin-like peptide
Short name:
Ley-I-L
Relaxin-like factor
Cleaved into the following 2 chains:
Gene namesi
Name:Insl3
Synonyms:Rlf
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 16

Organism-specific databases

RGDi620117. Insl3.

Subcellular locationi

GO - Cellular componenti

  • extracellular space Source: RGD
  • perinuclear region of cytoplasm Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei? – 100C peptide likeSequence analysisPRO_0000016153
Signal peptidei1 – 1515Sequence analysisAdd
BLAST
Peptidei16 – ?Insulin-like 3 B chainPRO_0000016152
Peptidei103 – 12826Insulin-like 3 A chainPRO_0000016154Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi29 ↔ 113Interchain (between B and A chains)By similarity
Disulfide bondi41 ↔ 126Interchain (between B and A chains)By similarity
Disulfide bondi112 ↔ 117By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

PaxDbiQ9WUK0.

Expressioni

Tissue specificityi

Expressed in Leydig cells of the testis, and weakly in the theca interna cells of antral follicles and the corpus luteum of the ovary.1 Publication

Developmental stagei

Highly expressed in adult testis and low expression in the ovary. Highly up-regulated in testes of day 19 embryos, but not in later neonatal stages, nor any ovarian tissue from this period.1 Publication

Gene expression databases

GenevisibleiQ9WUK0. RN.

Interactioni

Subunit structurei

Heterodimer of a B chain and an A chain linked by two disulfide bonds.By similarity

GO - Molecular functioni

  • G-protein coupled receptor binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025349.

Structurei

3D structure databases

ProteinModelPortaliQ9WUK0.
SMRiQ9WUK0. Positions 22-50.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the insulin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J0IS. Eukaryota.
ENOG411140J. LUCA.
GeneTreeiENSGT00510000050366.
HOGENOMiHOG000113077.
HOVERGENiHBG052133.
InParanoidiQ9WUK0.
KOiK05255.
OMAiPARHCCL.
OrthoDBiEOG7P5T43.
PhylomeDBiQ9WUK0.
TreeFamiTF106361.

Family and domain databases

Gene3Di1.10.100.10. 2 hits.
InterProiIPR016179. Insulin-like.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamiPF00049. Insulin. 1 hit.
[Graphical view]
PRINTSiPR00276. INSULINFAMLY.
SMARTiSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMiSSF56994. SSF56994. 2 hits.
PROSITEiPS00262. INSULIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9WUK0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHALLLLLLL ALGSALRSPQ PPEARAKLCG HHLVRALVRV CGGPRWSPEA
60 70 80 90 100
TQPVDTRDRE LLQWLEQRHL LHALVADADP ALDPDPALDP QLPHQASQRQ
110 120
RRSVATNAVH RCCLTGCTQQ DLLGLCPH
Length:128
Mass (Da):14,110
Last modified:August 22, 2003 - v2
Checksum:iABF2EE3AE30ADD53
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF139918 mRNA. Translation: AAD33663.2.
AF139920 Genomic DNA. Translation: AAD33851.1.
RefSeqiNP_446132.1. NM_053680.1.
UniGeneiRn.48700.

Genome annotation databases

EnsembliENSRNOT00000025347; ENSRNOP00000025349; ENSRNOG00000018757.
GeneIDi114215.
KEGGirno:114215.
UCSCiRGD:620117. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF139918 mRNA. Translation: AAD33663.2.
AF139920 Genomic DNA. Translation: AAD33851.1.
RefSeqiNP_446132.1. NM_053680.1.
UniGeneiRn.48700.

3D structure databases

ProteinModelPortaliQ9WUK0.
SMRiQ9WUK0. Positions 22-50.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025349.

Proteomic databases

PaxDbiQ9WUK0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000025347; ENSRNOP00000025349; ENSRNOG00000018757.
GeneIDi114215.
KEGGirno:114215.
UCSCiRGD:620117. rat.

Organism-specific databases

CTDi3640.
RGDi620117. Insl3.

Phylogenomic databases

eggNOGiENOG410J0IS. Eukaryota.
ENOG411140J. LUCA.
GeneTreeiENSGT00510000050366.
HOGENOMiHOG000113077.
HOVERGENiHBG052133.
InParanoidiQ9WUK0.
KOiK05255.
OMAiPARHCCL.
OrthoDBiEOG7P5T43.
PhylomeDBiQ9WUK0.
TreeFamiTF106361.

Enzyme and pathway databases

ReactomeiR-RNO-418555. G alpha (s) signalling events.
R-RNO-444821. Relaxin receptors.

Miscellaneous databases

NextBioi618433.
PROiQ9WUK0.

Gene expression databases

GenevisibleiQ9WUK0. RN.

Family and domain databases

Gene3Di1.10.100.10. 2 hits.
InterProiIPR016179. Insulin-like.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamiPF00049. Insulin. 1 hit.
[Graphical view]
PRINTSiPR00276. INSULINFAMLY.
SMARTiSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMiSSF56994. SSF56994. 2 hits.
PROSITEiPS00262. INSULIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Structure and expression of the rat relaxin-like factor (RLF) gene."
    Spiess A.-N., Balvers M., Tena-Sempere M., Huhtaniemi I., Parry L., Ivell R.
    Mol. Reprod. Dev. 54:319-325(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: Sprague-Dawley.
    Tissue: Testis.

Entry informationi

Entry nameiINSL3_RAT
AccessioniPrimary (citable) accession number: Q9WUK0
Secondary accession number(s): Q9WUK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 22, 2003
Last modified: May 11, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.