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Protein

Homeobox protein MIXL1

Gene

Mixl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor that play a central role in proper axial mesendoderm morphogenesis and endoderm formation. Required for efficient differentiation of cells from the primitive streak stage to blood, by acting early in the recruitment and/or expansion of mesodermal progenitors to the hemangioblastic and hematopoietic lineages. Also involved in the morphogenesis of the heart and the gut during embryogenesis. Acts as a negative regulator of brachyury expression.6 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi86 – 145HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein homodimerization activity Source: BHF-UCL
  • proximal promoter sequence-specific DNA binding Source: BHF-UCL
  • RNA polymerase II distal enhancer sequence-specific DNA binding Source: BHF-UCL
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: BHF-UCL
  • RNA polymerase II repressing transcription factor binding Source: BHF-UCL
  • RNA polymerase II transcription factor binding Source: BHF-UCL
  • transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific DNA binding Source: BHF-UCL
  • transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: BHF-UCL
  • transcriptional activator activity, RNA polymerase II transcription factor binding Source: BHF-UCL

GO - Biological processi

  • cell migration involved in gastrulation Source: MGI
  • digestive tract development Source: MGI
  • endodermal cell differentiation Source: BHF-UCL
  • endoderm development Source: MGI
  • endoderm formation Source: MGI
  • gastrulation Source: MGI
  • heart development Source: MGI
  • hematopoietic progenitor cell differentiation Source: BHF-UCL
  • hemopoiesis Source: MGI
  • negative regulation of hematopoietic progenitor cell differentiation Source: BHF-UCL
  • positive regulation of mesoderm development Source: BHF-UCL
  • positive regulation of transcription by RNA polymerase II Source: BHF-UCL

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processDifferentiation, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein MIXL1
Alternative name(s):
Homeodomain protein MIX
Short name:
mMix
MIX1 homeobox-like protein 1
Mix.1 homeobox-like protein
Gene namesi
Name:Mixl1
Synonyms:Mix, Mml
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1351322 Mixl1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice display defects in mesendoderm morphogenesis and patterning during development. Embryos show a marked thickening of the primitive streak. By the early somite stage, embryonic development is arrested, with the formation of abnormal head folds, foreshortened body axis, absence of heart tube and gut, deficient paraxial mesoderm, and an enlarged midline tissue mass that replaces the notochord. Development of extra-embryonic structures is generally normal except that the allantois is often disproportionately large for the size of the mutant embryo.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003113341 – 231Homeobox protein MIXL1Add BLAST231

Proteomic databases

PaxDbiQ9WUI0
PeptideAtlasiQ9WUI0
PRIDEiQ9WUI0

PTM databases

PhosphoSitePlusiQ9WUI0

Expressioni

Tissue specificityi

Expressed in the primitive streak of the gastrulating embryo, and marks cells destined to form mesoderm and endoderm. Present in differentiating embryonic stem cells (at protein level).2 Publications

Developmental stagei

Initially expressed in visceral endoderm and becomes restricted to primitive streak and nascent mesoderm at gastrulation. This includes the hemangioblast, a precursor of hematopoietic and vascular stem cells. At E5.5, it is expressed symmetrically in the visceral endoderm but by E6.0 this expression is noticeably asymmetric. At E6.5, expression is restricted to the nascent primitive streak and persists in the primitive streak through E7.5-E9.5, marking those cells fated to form extra-embryonic and lateral mesoderm.2 Publications

Inductioni

By TGF-beta. Regulated by Nodal in mesendoderm morphogenesis. Regulated by FOXH1, which acts as a negative regulator by recruiting GSC to promoter during early development.2 Publications

Gene expression databases

BgeeiENSMUSG00000026497
CleanExiMM_MIXL1
GenevisibleiQ9WUI0 MM

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein homodimerization activity Source: BHF-UCL
  • RNA polymerase II repressing transcription factor binding Source: BHF-UCL
  • RNA polymerase II transcription factor binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi20513810 interactors.
IntActiQ9WUI0 6 interactors.
STRINGi10090.ENSMUSP00000027778

Structurei

3D structure databases

ProteinModelPortaliQ9WUI0
SMRiQ9WUI0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the paired homeobox family.Curated

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0849 Eukaryota
ENOG410XS01 LUCA
GeneTreeiENSGT00860000133740
HOGENOMiHOG000113594
HOVERGENiHBG106595
InParanoidiQ9WUI0
OMAiTMYPDIH
OrthoDBiEOG091G15I5
PhylomeDBiQ9WUI0
TreeFamiTF334098

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9WUI0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAGSQQLQ FAEGAAFPIF PAAHPGGQLL PAMRPASGLP AAPHDSRAPA
60 70 80 90 100
ATQCFPNRDS SPTAQTPAGL DPPGPSKGSA APSAPQRRKR TSFSSEQLQL
110 120 130 140 150
LELVFRQTMY PDIHLRERLA ALTLLPESRI QVWFQNRRAK SRRQSGKSFQ
160 170 180 190 200
PLSSRRGVFL HCPAPGTEAR CLKPQLPLEA DVNHVPDPSM TGGGVCTSGS
210 220 230
QSFETYSSLS EDIGSKLDSW EEHIFSALGN F
Length:231
Mass (Da):24,844
Last modified:November 1, 1999 - v1
Checksum:i59DA80A5E7E6EE33
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF135063 mRNA Translation: AAD25543.1
AF154573 mRNA Translation: AAK59867.1
AF201959 mRNA Translation: AAF08314.1
AF447591 Genomic DNA Translation: AAM77218.1
BC099876 mRNA Translation: AAH99876.1
BC099877 mRNA Translation: AAH99877.1
CCDSiCCDS15571.1
RefSeqiNP_038757.1, NM_013729.3
XP_006496934.1, XM_006496871.1
UniGeneiMm.103647

Genome annotation databases

EnsembliENSMUST00000027778; ENSMUSP00000027778; ENSMUSG00000026497
GeneIDi27217
KEGGimmu:27217
UCSCiuc007dwp.1 mouse

Similar proteinsi

Entry informationi

Entry nameiMIXL1_MOUSE
AccessioniPrimary (citable) accession number: Q9WUI0
Secondary accession number(s): Q9QZ61
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: November 1, 1999
Last modified: March 28, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome