Q9WUG6 (INSL5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Insulin-like peptide INSL5
Short name=Insulin-like peptide 5

Alternative name(s):
Relaxin/insulin-like factor 2
Relaxin/insulin-like protein

Cleaved into the following 2 chains:

1. Insulin-like peptide INSL5 B chain
2. Insulin-like peptide INSL5 A chain

Gene names

Name:	Insl5
Synonyms:	Rif, Rif2, Zins3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	135 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May have a role in gut contractility or in thymic development and regulation. Activates RXFP4 with high potency and appears to be the endogenous ligand for this receptor By similarity.
Subunit structure	Heterodimer of a B chain and an A chain linked by two disulfide bonds By similarity.
Subcellular location	Secreted By similarity.
Tissue specificity	Highest expression in colon with lower levels in thymus. Minimal levels in testis. Ref.1
Sequence similarities	Belongs to the insulin family.
Sequence caution	The sequence AAF09093.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hormone
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 46	24	Insulin-like peptide INSL5 B chain		PRO_0000016166
	Propeptide	49 – 114	66	Connecting peptide Potential		PRO_0000016167
	Peptide	115 – 135	21	Insulin-like peptide INSL5 A chain		PRO_0000016168
Amino acid modifications
	Disulfide bond	29 ↔ 122		Interchain (between B and A chains) By similarity
	Disulfide bond	41 ↔ 135		Interchain (between B and A chains) By similarity
	Disulfide bond	121 ↔ 126		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9WUG6 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 05FF9A0F613DBF92	FASTA	135	15,524
	10 20 30 40 50 60 MKGPTLALFL LLVLLAVVEV RSRQTVKLCG LDYVRTVIYI CASSRWRRHL EGHFHSQQAE 70 80 90 100 110 120 TRNYLQLLDR HEPSKKTLEH SLPKTDLSGQ ELVRDPQAPK EGLWELKKHS VVSRRDLQAL 130 CCREGCSMKE LSTLC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of INSL5, a new member of the insulin superfamily." Conklin D., Lofton-Day C.E., Haldeman B.A., Ching A., Whitmore T.E., Lok S., Jaspers S. Genomics 60:50-56(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Colon.
[2]	"Cloning of two novel mammalian paralogs of relaxin/insulin family proteins and their expression in testis and kidney." Hsu S.Y. Mol. Endocrinol. 13:2163-2174(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: FVB. Tissue: Ovary.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF133817 mRNA. Translation: AAD29687.1. AF076971 mRNA. Translation: AAD48089.1. AF054842 mRNA. Translation: AAF09093.1. Different initiation. AF054843 mRNA. Translation: AAF09094.1. BC010968 mRNA. Translation: AAH10968.1.
IPI	IPI00124628.
RefSeq	NP_035961.1. NM_011831.2.
UniGene	Mm.100163.
3D structure databases
ProteinModelPortal	Q9WUG6.
SMR	Q9WUG6. Positions 20-46, 110-135.
ModBase	Search...
Proteomic databases
PRIDE	Q9WUG6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000084382; ENSMUSP00000081416; ENSMUSG00000066090.
GeneID	23919.
KEGG	mmu:23919.
Organism-specific databases
CTD	10022.
MGI	MGI:1346085. Insl5.
Phylogenomic databases
eggNOG	NOG38979.
GeneTree	ENSGT00510000049372.
HOGENOM	HOG000111888.
HOVERGEN	HBG052135.
InParanoid	Q9WUG6.
OrthoDB	EOG40S0GZ.
Gene expression databases
ArrayExpress	Q9WUG6.
Bgee	Q9WUG6.
CleanEx	MM_INSL5.
Genevestigator	Q9WUG6.
GermOnline	ENSMUSG00000066090. Mus musculus.
Family and domain databases
Gene3D	1.10.100.10. 2 hits.
InterPro	IPR016179. Insulin-like. IPR022353. Insulin_CS. [Graphical view]
Pfam	PF00049. Insulin. 1 hit. [Graphical view]
SMART	SM00078. IlGF. 1 hit. [Graphical view]
SUPFAM	SSF56994. Insulin-like. 1 hit.
PROSITE	PS00262. INSULIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	303693.
SOURCE	Search...

Entry information

Entry name

INSL5_MOUSE

Accession

Primary (citable) accession number: Q9WUG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	April 3, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program