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Protein

Insulin-like peptide INSL5

Gene

Insl5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

May have a role in gut contractility or in thymic development and regulation. Activates RXFP4 with high potency and appears to be the endogenous ligand for this receptor (By similarity).By similarity

Keywords - Molecular functioni

Hormone

Enzyme and pathway databases

ReactomeiREACT_198713. Relaxin receptors.
REACT_250376. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin-like peptide INSL5
Short name:
Insulin-like peptide 5
Alternative name(s):
Relaxin/insulin-like factor 2
Relaxin/insulin-like protein
Cleaved into the following 2 chains:
Gene namesi
Name:Insl5
Synonyms:Rif, Rif2, Zins3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:1346085. Insl5.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 4624Insulin-like peptide INSL5 B chainPRO_0000016166Add
BLAST
Propeptidei49 – 11466Connecting peptideSequence AnalysisPRO_0000016167Add
BLAST
Peptidei115 – 13521Insulin-like peptide INSL5 A chainPRO_0000016168Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi29 ↔ 122Interchain (between B and A chains)By similarity
Disulfide bondi41 ↔ 135Interchain (between B and A chains)By similarity
Disulfide bondi121 ↔ 126By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

PRIDEiQ9WUG6.

Expressioni

Tissue specificityi

Highest expression in colon with lower levels in thymus. Minimal levels in testis.1 Publication

Gene expression databases

BgeeiQ9WUG6.
CleanExiMM_INSL5.
ExpressionAtlasiQ9WUG6. baseline and differential.
GenevestigatoriQ9WUG6.

Interactioni

Subunit structurei

Heterodimer of a B chain and an A chain linked by two disulfide bonds.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9WUG6.
SMRiQ9WUG6. Positions 23-51, 110-135.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the insulin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG38979.
GeneTreeiENSGT00510000049372.
HOGENOMiHOG000111888.
HOVERGENiHBG052135.
InParanoidiQ9WUG6.
OrthoDBiEOG73V6N0.
TreeFamiTF333404.

Family and domain databases

Gene3Di1.10.100.10. 2 hits.
InterProiIPR016179. Insulin-like.
IPR022353. Insulin_CS.
[Graphical view]
PfamiPF00049. Insulin. 1 hit.
[Graphical view]
SMARTiSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMiSSF56994. SSF56994. 2 hits.
PROSITEiPS00262. INSULIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9WUG6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKGPTLALFL LLVLLAVVEV RSRQTVKLCG LDYVRTVIYI CASSRWRRHL
60 70 80 90 100
EGHFHSQQAE TRNYLQLLDR HEPSKKTLEH SLPKTDLSGQ ELVRDPQAPK
110 120 130
EGLWELKKHS VVSRRDLQAL CCREGCSMKE LSTLC
Length:135
Mass (Da):15,524
Last modified:November 1, 1999 - v1
Checksum:i05FF9A0F613DBF92
GO

Sequence cautioni

The sequence AAF09093.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF133817 mRNA. Translation: AAD29687.1.
AF076971 mRNA. Translation: AAD48089.1.
AF054842 mRNA. Translation: AAF09093.1. Different initiation.
AF054843 mRNA. Translation: AAF09094.1.
BC010968 mRNA. Translation: AAH10968.1.
CCDSiCCDS18406.1.
RefSeqiNP_001277577.1. NM_001290648.1.
NP_035961.1. NM_011831.2.
UniGeneiMm.100163.

Genome annotation databases

EnsembliENSMUST00000084382; ENSMUSP00000081416; ENSMUSG00000066090.
GeneIDi23919.
KEGGimmu:23919.
UCSCiuc008txa.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF133817 mRNA. Translation: AAD29687.1.
AF076971 mRNA. Translation: AAD48089.1.
AF054842 mRNA. Translation: AAF09093.1. Different initiation.
AF054843 mRNA. Translation: AAF09094.1.
BC010968 mRNA. Translation: AAH10968.1.
CCDSiCCDS18406.1.
RefSeqiNP_001277577.1. NM_001290648.1.
NP_035961.1. NM_011831.2.
UniGeneiMm.100163.

3D structure databases

ProteinModelPortaliQ9WUG6.
SMRiQ9WUG6. Positions 23-51, 110-135.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ9WUG6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000084382; ENSMUSP00000081416; ENSMUSG00000066090.
GeneIDi23919.
KEGGimmu:23919.
UCSCiuc008txa.1. mouse.

Organism-specific databases

CTDi10022.
MGIiMGI:1346085. Insl5.

Phylogenomic databases

eggNOGiNOG38979.
GeneTreeiENSGT00510000049372.
HOGENOMiHOG000111888.
HOVERGENiHBG052135.
InParanoidiQ9WUG6.
OrthoDBiEOG73V6N0.
TreeFamiTF333404.

Enzyme and pathway databases

ReactomeiREACT_198713. Relaxin receptors.
REACT_250376. G alpha (i) signalling events.

Miscellaneous databases

NextBioi303693.
PROiQ9WUG6.
SOURCEiSearch...

Gene expression databases

BgeeiQ9WUG6.
CleanExiMM_INSL5.
ExpressionAtlasiQ9WUG6. baseline and differential.
GenevestigatoriQ9WUG6.

Family and domain databases

Gene3Di1.10.100.10. 2 hits.
InterProiIPR016179. Insulin-like.
IPR022353. Insulin_CS.
[Graphical view]
PfamiPF00049. Insulin. 1 hit.
[Graphical view]
SMARTiSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMiSSF56994. SSF56994. 2 hits.
PROSITEiPS00262. INSULIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of INSL5, a new member of the insulin superfamily."
    Conklin D., Lofton-Day C.E., Haldeman B.A., Ching A., Whitmore T.E., Lok S., Jaspers S.
    Genomics 60:50-56(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Colon.
  2. "Cloning of two novel mammalian paralogs of relaxin/insulin family proteins and their expression in testis and kidney."
    Hsu S.Y.
    Mol. Endocrinol. 13:2163-2174(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: FVB.
    Tissue: Ovary.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Colon.

Entry informationi

Entry nameiINSL5_MOUSE
AccessioniPrimary (citable) accession number: Q9WUG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: January 7, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.