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Protein

Vesicle-associated membrane protein 8

Gene

Vamp8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex (By similarity). Also required for dense-granule secretion in platelets (By similarity). Plays also a role in regulated enzyme secretion in pancreatic acinar cells (By similarity). Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells (PubMed:12737809). Involved in the homotypic fusion of early and late endosomes (PubMed:10982406, PubMed:11029036).By similarity3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei32 – 321Binding to STX81 Publication

GO - Molecular functioni

  • SNAP receptor activity Source: FlyBase
  • syntaxin binding Source: RGD

GO - Biological processi

  • autophagosome maturation Source: UniProtKB
  • membrane fusion Source: RGD
  • protein complex assembly Source: RGD
  • protein transport Source: UniProtKB-KW
  • vesicle fusion Source: RGD
  • vesicle-mediated transport Source: RGD
Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 81 Publication
Short name:
VAMP-81 Publication
Alternative name(s):
EndobrevinBy similarity
Short name:
EDBBy similarity
Gene namesi
Name:Vamp81 Publication
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620421. Vamp8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7474CytoplasmicSequence analysisAdd
BLAST
Transmembranei75 – 9521Helical; Anchor for type IV membrane proteinSequence analysisAdd
BLAST
Topological domaini96 – 1005VesicularSequence analysis

GO - Cellular componenti

  • early endosome membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • late endosome membrane Source: RGD
  • lysosomal membrane Source: RGD
  • midbody Source: UniProtKB-SubCell
  • plasma membrane Source: UniProtKB-SubCell
  • recycling endosome Source: RGD
  • SNARE complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 100100Vesicle-associated membrane protein 8PRO_0000206739Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei53 – 531PhosphothreonineBy similarity
Modified residuei54 – 541PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9WUF4.
PRIDEiQ9WUF4.

Expressioni

Tissue specificityi

Expressed (at protein level) at a high level in kidney, lung and spleen; at a lower level in testis, liver, brain and heart. Expressed in kidney and retinal pigment epithelium derived cell line.2 Publications

Interactioni

Subunit structurei

Forms a SNARE complex composed of VAMP8, SNAP29 and STX17 involved in fusion of autophagosome with lysosome (By similarity). Found in a number of SNARE complexes with NAPA, SNAP23, SNAP25, STX1A, STX4, STX7, STX8 and VTI1B (PubMed:10336434, PubMed:9614193, PubMed:11786915).By similarity3 Publications

GO - Molecular functioni

  • SNAP receptor activity Source: FlyBase
  • syntaxin binding Source: RGD

Protein-protein interaction databases

DIPiDIP-43964N.
IntActiQ9WUF4. 7 interactions.
MINTiMINT-4570149.
STRINGi10116.ENSRNOP00000017301.

Structurei

Secondary structure

1
100
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi12 – 6251Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GL2X-ray1.90A6-66[»]
ProteinModelPortaliQ9WUF4.
SMRiQ9WUF4. Positions 11-63.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WUF4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 7161v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated
Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiQ9WUF4.
KOiK08512.
PhylomeDBiQ9WUF4.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WUF4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEASGSAGND RVRNLQSEVE GVKNIMTQNV ERILARGENL DHLRNKTEDL
60 70 80 90 100
EATSEHFKTT SQKVARKFWW KNVKMIVIIC VIVLIILILI ILFATGTIPT
Length:100
Mass (Da):11,320
Last modified:November 1, 1999 - v1
Checksum:iD4449EAF44F03672
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132812 mRNA. Translation: AAD33595.1.
RefSeqiNP_114015.1. NM_031827.1.
UniGeneiRn.82672.

Genome annotation databases

GeneIDi83730.
KEGGirno:83730.
UCSCiRGD:620421. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132812 mRNA. Translation: AAD33595.1.
RefSeqiNP_114015.1. NM_031827.1.
UniGeneiRn.82672.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GL2X-ray1.90A6-66[»]
ProteinModelPortaliQ9WUF4.
SMRiQ9WUF4. Positions 11-63.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-43964N.
IntActiQ9WUF4. 7 interactions.
MINTiMINT-4570149.
STRINGi10116.ENSRNOP00000017301.

Proteomic databases

PaxDbiQ9WUF4.
PRIDEiQ9WUF4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi83730.
KEGGirno:83730.
UCSCiRGD:620421. rat.

Organism-specific databases

CTDi8673.
RGDi620421. Vamp8.

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiQ9WUF4.
KOiK08512.
PhylomeDBiQ9WUF4.

Miscellaneous databases

EvolutionaryTraceiQ9WUF4.
NextBioi616321.
PROiQ9WUF4.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Mixed and non-cognate SNARE complexes. Characterization of assembly and biophysical properties."
    Fasshauer D., Antonin W., Margittai M., Pabst S., Jahn R.
    J. Biol. Chem. 274:15440-15446(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION IN A TERNARY COMPLEX WITH STX1A AND SNAP25.
  2. "Endobrevin, a novel synaptobrevin/VAMP-like protein preferentially associated with the early endosome."
    Wong S.H., Zhang T., Xu Y., Subramaniam V.N., Griffiths G., Hong W.
    Mol. Biol. Cell 9:1549-1563(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN A COMPLEX WITH NAPA.
  3. "SNARE protein trafficking in polarized MDCK cells."
    Steegmaier M., Lee K.C., Prekeris R., Scheller R.H.
    Traffic 1:553-560(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  4. "Syntaxin 7 is localized to late endosome compartments, associates with Vamp 8, and Is required for late endosome-lysosome fusion."
    Mullock B.M., Smith C.W., Ihrke G., Bright N.A., Lindsay M., Parkinson E.J., Brooks D.A., Parton R.G., James D.E., Luzio J.P., Piper R.C.
    Mol. Biol. Cell 11:3137-3153(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH STX7, SUBCELLULAR LOCATION, FUNCTION.
  5. "The R-SNARE endobrevin/VAMP-8 mediates homotypic fusion of early endosomes and late endosomes."
    Antonin W., Holroyd C., Tikkanen R., Honing S., Jahn R.
    Mol. Biol. Cell 11:3289-3298(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  6. "Retinal pigment epithelial cells exhibit unique expression and localization of plasma membrane syntaxins which may contribute to their trafficking phenotype."
    Low S.H., Marmorstein L.Y., Miura M., Li X., Kudo N., Marmorstein A.D., Weimbs T.
    J. Cell Sci. 115:4545-4553(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  7. "Syntaxin 2 and endobrevin are required for the terminal step of cytokinesis in mammalian cells."
    Low S.H., Li X., Miura M., Kudo N., Quinones B., Weimbs T.
    Dev. Cell 4:753-759(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  8. "Crystal structure of the endosomal SNARE complex reveals common structural principles of all SNAREs."
    Antonin W., Fasshauer D., Becker S., Jahn R., Schneider T.R.
    Nat. Struct. Biol. 9:107-111(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 6-66 IN COMPLEX WITH STX7; STX8 AND VTI1B, INTERACTION WITH STX8.

Entry informationi

Entry nameiVAMP8_RAT
AccessioniPrimary (citable) accession number: Q9WUF4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: November 1, 1999
Last modified: February 17, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.