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Protein

Vesicle-associated membrane protein 8

Gene

Vamp8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t-SNARE complex (By similarity). Also required for dense-granule secretion in platelets (By similarity). Plays also a role in regulated enzyme secretion in pancreatic acinar cells (By similarity). Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells (PubMed:12737809). Involved in the homotypic fusion of early and late endosomes (PubMed:10982406, PubMed:11029036).By similarity3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei32Binding to STX81 Publication1

GO - Molecular functioni

  • SNAP receptor activity Source: FlyBase
  • syntaxin binding Source: RGD

GO - Biological processi

  • autophagosome maturation Source: UniProtKB
  • exocytosis Source: GO_Central
  • membrane fusion Source: RGD
  • protein complex assembly Source: RGD
  • protein transport Source: UniProtKB-KW
  • vesicle fusion Source: RGD
  • vesicle-mediated transport Source: RGD
Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 81 Publication
Short name:
VAMP-81 Publication
Alternative name(s):
EndobrevinBy similarity
Short name:
EDBBy similarity
Gene namesi
Name:Vamp81 Publication
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620421. Vamp8.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 74CytoplasmicSequence analysisAdd BLAST74
Transmembranei75 – 95Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini96 – 100VesicularSequence analysis5

GO - Cellular componenti

  • early endosome membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • late endosome membrane Source: RGD
  • lysosomal membrane Source: RGD
  • midbody Source: UniProtKB-SubCell
  • plasma membrane Source: UniProtKB-SubCell
  • recycling endosome Source: RGD
  • SNARE complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002067391 – 100Vesicle-associated membrane protein 8Add BLAST100

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4PhosphoserineBy similarity1
Modified residuei17PhosphoserineBy similarity1
Modified residuei27PhosphothreonineBy similarity1
Modified residuei47PhosphothreonineBy similarity1
Modified residuei53PhosphothreonineBy similarity1
Modified residuei54PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9WUF4.
PRIDEiQ9WUF4.

PTM databases

iPTMnetiQ9WUF4.
PhosphoSitePlusiQ9WUF4.

Expressioni

Tissue specificityi

Expressed (at protein level) at a high level in kidney, lung and spleen; at a lower level in testis, liver, brain and heart. Expressed in kidney and retinal pigment epithelium derived cell line.2 Publications

Gene expression databases

BgeeiENSRNOG00000012748.

Interactioni

Subunit structurei

Forms a SNARE complex composed of VAMP8, SNAP29 and STX17 involved in fusion of autophagosome with lysosome (By similarity). Found in a number of SNARE complexes with NAPA, SNAP23, SNAP25, STX1A, STX4, STX7, STX8 and VTI1B (PubMed:10336434, PubMed:9614193, PubMed:11786915).By similarity3 Publications

GO - Molecular functioni

  • SNAP receptor activity Source: FlyBase
  • syntaxin binding Source: RGD

Protein-protein interaction databases

DIPiDIP-43964N.
IntActiQ9WUF4. 7 interactors.
MINTiMINT-4570149.
STRINGi10116.ENSRNOP00000017301.

Structurei

Secondary structure

1100
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 62Combined sources51

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GL2X-ray1.90A6-66[»]
ProteinModelPortaliQ9WUF4.
SMRiQ9WUF4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WUF4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 71v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST61

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated
Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiQ9WUF4.
KOiK08512.
PhylomeDBiQ9WUF4.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WUF4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEASGSAGND RVRNLQSEVE GVKNIMTQNV ERILARGENL DHLRNKTEDL
60 70 80 90 100
EATSEHFKTT SQKVARKFWW KNVKMIVIIC VIVLIILILI ILFATGTIPT
Length:100
Mass (Da):11,320
Last modified:November 1, 1999 - v1
Checksum:iD4449EAF44F03672
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132812 mRNA. Translation: AAD33595.1.
RefSeqiNP_114015.1. NM_031827.1.
UniGeneiRn.82672.

Genome annotation databases

GeneIDi83730.
KEGGirno:83730.
UCSCiRGD:620421. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132812 mRNA. Translation: AAD33595.1.
RefSeqiNP_114015.1. NM_031827.1.
UniGeneiRn.82672.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GL2X-ray1.90A6-66[»]
ProteinModelPortaliQ9WUF4.
SMRiQ9WUF4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-43964N.
IntActiQ9WUF4. 7 interactors.
MINTiMINT-4570149.
STRINGi10116.ENSRNOP00000017301.

PTM databases

iPTMnetiQ9WUF4.
PhosphoSitePlusiQ9WUF4.

Proteomic databases

PaxDbiQ9WUF4.
PRIDEiQ9WUF4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi83730.
KEGGirno:83730.
UCSCiRGD:620421. rat.

Organism-specific databases

CTDi8673.
RGDi620421. Vamp8.

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiQ9WUF4.
KOiK08512.
PhylomeDBiQ9WUF4.

Miscellaneous databases

EvolutionaryTraceiQ9WUF4.
PROiQ9WUF4.

Gene expression databases

BgeeiENSRNOG00000012748.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVAMP8_RAT
AccessioniPrimary (citable) accession number: Q9WUF4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.