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Q9WUC6 (TIMP2_CAVPO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Metalloproteinase inhibitor 2
Alternative name(s):
Tissue inhibitor of metalloproteinases 2
Short name=TIMP-2
Gene names
Name:TIMP2
OrganismCavia porcellus (Guinea pig) [Reference proteome]
Taxonomic identifier10141 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia

Protein attributes

Sequence length220 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.

Subunit structure

Interacts (via the C-terminal) with MMP2 (via the C-terminal PEX domain); the interaction inhibits the MMP2 activity By similarity.

Subcellular location

Secreted.

Tissue specificity

Predominantly expressed in the lung in alveolar macrophages and epithelial cells. Also found in brain, kidney, intestine, spleen and heart.

Post-translational modification

The activity of TIMP2 is dependent on the presence of disulfide bonds.

Sequence similarities

Belongs to the protease inhibitor I35 (TIMP) family. [View classification]

Contains 1 NTR domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 220194Metalloproteinase inhibitor 2
PRO_0000034333

Regions

Domain27 – 152126NTR
Region27 – 315Involved in metalloproteinase-binding By similarity
Region95 – 962Involved in metalloproteinase-binding By similarity

Sites

Metal binding271Zinc; via amino nitrogen and carbonyl oxygen; shared with metalloproteinase partner By similarity

Amino acid modifications

Disulfide bond27 ↔ 98 By similarity
Disulfide bond29 ↔ 127 By similarity
Disulfide bond39 ↔ 152 By similarity
Disulfide bond154 ↔ 201 By similarity
Disulfide bond159 ↔ 164 By similarity
Disulfide bond172 ↔ 193 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9WUC6 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 2A2541764755B9AF

FASTA22024,318
        10         20         30         40         50         60 
MGATARSLRL ALGLLLLGTL PRGADACSCS PVHPQQAFCN ADVVIRAKAV SEKEVDSGND 

        70         80         90        100        110        120 
IYGNPIKRIQ YEIKQIKMFK GPEKDIEFIY TAPSSAVCGV SLDVGGKKEY LIAGKAEGDG 

       130        140        150        160        170        180 
KMHITLCDFI VPWDTLSTTQ KKSLNHRYQM GCECKITRCP MIPCYISSPD ECLWMDWVTE 

       190        200        210        220 
KSINGHQAKF FACIKRSDGS CAWYRGAAPP KQEFLDIEDP 

« Hide

References

[1]"Cloning and expression of guinea pig TIMP-2. Expression in normal and hyperoxic lung injury."
Melendez J., Maldonado V., Selman M., Pardo A.
Am. J. Physiol. 278:L737-L743(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Hartley.
Tissue: Lung.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF127803 mRNA. Translation: AAD28252.1.
RefSeqNP_001166495.1. NM_001173024.1.

3D structure databases

ProteinModelPortalQ9WUC6.
SMRQ9WUC6. Positions 27-218.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10141.ENSCPOP00000007780.

Protein family/group databases

MEROPSI35.002.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSCPOT00000008741; ENSCPOP00000007780; ENSCPOG00000008663.
GeneID100135629.

Organism-specific databases

CTD7077.

Phylogenomic databases

eggNOGNOG243625.
GeneTreeENSGT00390000004555.
HOGENOMHOG000285981.
HOVERGENHBG068749.
InParanoidQ9WUC6.
OMASAPEECL.
OrthoDBEOG79GT74.
TreeFamTF317409.

Family and domain databases

Gene3D3.90.370.10. 1 hit.
InterProIPR001134. Netrin_domain.
IPR001820. Prot_inh_TIMP.
IPR008993. TIMP-like_OB-fold.
IPR015613. TIMP2.
IPR027465. TIMP_C_dom.
[Graphical view]
PANTHERPTHR11844. PTHR11844. 1 hit.
PTHR11844:SF7. PTHR11844:SF7. 1 hit.
PfamPF00965. TIMP. 1 hit.
[Graphical view]
SMARTSM00206. NTR. 1 hit.
[Graphical view]
SUPFAMSSF50242. SSF50242. 1 hit.
PROSITEPS50189. NTR. 1 hit.
PS00288. TIMP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTIMP2_CAVPO
AccessionPrimary (citable) accession number: Q9WUC6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1999
Last modified: May 14, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families