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Q9WUC6

- TIMP2_CAVPO

UniProt

Q9WUC6 - TIMP2_CAVPO

Protein

Metalloproteinase inhibitor 2

Gene

TIMP2

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi27 – 271Zinc; via amino nitrogen and carbonyl oxygen; shared with metalloproteinase partnerBy similarity

    GO - Molecular functioni

    1. enzyme activator activity Source: Ensembl
    2. metal ion binding Source: UniProtKB-KW
    3. metalloendopeptidase inhibitor activity Source: UniProtKB-KW

    GO - Biological processi

    1. cellular response to organic substance Source: Ensembl
    2. negative regulation of cell proliferation Source: Ensembl
    3. regulation of cAMP metabolic process Source: Ensembl
    4. regulation of MAPK cascade Source: Ensembl
    5. regulation of neuron differentiation Source: Ensembl

    Keywords - Molecular functioni

    Metalloenzyme inhibitor, Metalloprotease inhibitor, Protease inhibitor

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiI35.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Metalloproteinase inhibitor 2
    Alternative name(s):
    Tissue inhibitor of metalloproteinases 2
    Short name:
    TIMP-2
    Gene namesi
    Name:TIMP2
    OrganismiCavia porcellus (Guinea pig)
    Taxonomic identifieri10141 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
    ProteomesiUP000005447: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. basement membrane Source: Ensembl
    2. cell surface Source: Ensembl

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Sequence AnalysisAdd
    BLAST
    Chaini27 – 220194Metalloproteinase inhibitor 2PRO_0000034333Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi27 ↔ 98PROSITE-ProRule annotation
    Disulfide bondi29 ↔ 127PROSITE-ProRule annotation
    Disulfide bondi39 ↔ 152PROSITE-ProRule annotation
    Disulfide bondi154 ↔ 201PROSITE-ProRule annotation
    Disulfide bondi159 ↔ 164PROSITE-ProRule annotation
    Disulfide bondi172 ↔ 193PROSITE-ProRule annotation

    Post-translational modificationi

    The activity of TIMP2 is dependent on the presence of disulfide bonds.

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Tissue specificityi

    Predominantly expressed in the lung in alveolar macrophages and epithelial cells. Also found in brain, kidney, intestine, spleen and heart.

    Interactioni

    Subunit structurei

    Interacts (via the C-terminal) with MMP2 (via the C-terminal PEX domain); the interaction inhibits the MMP2 activity.By similarity

    Protein-protein interaction databases

    STRINGi10141.ENSCPOP00000007780.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9WUC6.
    SMRiQ9WUC6. Positions 27-218.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini27 – 152126NTRPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni27 – 315Involved in metalloproteinase-bindingBy similarity
    Regioni95 – 962Involved in metalloproteinase-bindingBy similarity

    Sequence similaritiesi

    Contains 1 NTR domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG243625.
    GeneTreeiENSGT00390000004555.
    HOGENOMiHOG000285981.
    HOVERGENiHBG068749.
    InParanoidiQ9WUC6.
    OMAiSAPEECL.
    OrthoDBiEOG79GT74.
    TreeFamiTF317409.

    Family and domain databases

    Gene3Di3.90.370.10. 1 hit.
    InterProiIPR001134. Netrin_domain.
    IPR001820. Prot_inh_TIMP.
    IPR008993. TIMP-like_OB-fold.
    IPR015613. TIMP2.
    IPR027465. TIMP_C_dom.
    [Graphical view]
    PANTHERiPTHR11844. PTHR11844. 1 hit.
    PTHR11844:SF7. PTHR11844:SF7. 1 hit.
    PfamiPF00965. TIMP. 1 hit.
    [Graphical view]
    SMARTiSM00206. NTR. 1 hit.
    [Graphical view]
    SUPFAMiSSF50242. SSF50242. 1 hit.
    PROSITEiPS50189. NTR. 1 hit.
    PS00288. TIMP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9WUC6-1 [UniParc]FASTAAdd to Basket

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    MGATARSLRL ALGLLLLGTL PRGADACSCS PVHPQQAFCN ADVVIRAKAV    50
    SEKEVDSGND IYGNPIKRIQ YEIKQIKMFK GPEKDIEFIY TAPSSAVCGV 100
    SLDVGGKKEY LIAGKAEGDG KMHITLCDFI VPWDTLSTTQ KKSLNHRYQM 150
    GCECKITRCP MIPCYISSPD ECLWMDWVTE KSINGHQAKF FACIKRSDGS 200
    CAWYRGAAPP KQEFLDIEDP 220
    Length:220
    Mass (Da):24,318
    Last modified:November 1, 1999 - v1
    Checksum:i2A2541764755B9AF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF127803 mRNA. Translation: AAD28252.1.
    RefSeqiNP_001166495.1. NM_001173024.1.

    Genome annotation databases

    EnsembliENSCPOT00000008741; ENSCPOP00000007780; ENSCPOG00000008663.
    GeneIDi100135629.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF127803 mRNA. Translation: AAD28252.1 .
    RefSeqi NP_001166495.1. NM_001173024.1.

    3D structure databases

    ProteinModelPortali Q9WUC6.
    SMRi Q9WUC6. Positions 27-218.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10141.ENSCPOP00000007780.

    Protein family/group databases

    MEROPSi I35.002.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSCPOT00000008741 ; ENSCPOP00000007780 ; ENSCPOG00000008663 .
    GeneIDi 100135629.

    Organism-specific databases

    CTDi 7077.

    Phylogenomic databases

    eggNOGi NOG243625.
    GeneTreei ENSGT00390000004555.
    HOGENOMi HOG000285981.
    HOVERGENi HBG068749.
    InParanoidi Q9WUC6.
    OMAi SAPEECL.
    OrthoDBi EOG79GT74.
    TreeFami TF317409.

    Family and domain databases

    Gene3Di 3.90.370.10. 1 hit.
    InterProi IPR001134. Netrin_domain.
    IPR001820. Prot_inh_TIMP.
    IPR008993. TIMP-like_OB-fold.
    IPR015613. TIMP2.
    IPR027465. TIMP_C_dom.
    [Graphical view ]
    PANTHERi PTHR11844. PTHR11844. 1 hit.
    PTHR11844:SF7. PTHR11844:SF7. 1 hit.
    Pfami PF00965. TIMP. 1 hit.
    [Graphical view ]
    SMARTi SM00206. NTR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50242. SSF50242. 1 hit.
    PROSITEi PS50189. NTR. 1 hit.
    PS00288. TIMP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of guinea pig TIMP-2. Expression in normal and hyperoxic lung injury."
      Melendez J., Maldonado V., Selman M., Pardo A.
      Am. J. Physiol. 278:L737-L743(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Hartley.
      Tissue: Lung.

    Entry informationi

    Entry nameiTIMP2_CAVPO
    AccessioniPrimary (citable) accession number: Q9WUC6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3