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Protein

Chloride channel protein ClC-Ka

Gene

Clcnka

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. May be important in urinary concentrating mechanisms. May be the basolateral chloride channel mediating net chloride absorption in CTAL cells.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi259CalciumBy similarity1
Metal bindingi261CalciumBy similarity1
Metal bindingi278CalciumBy similarity1
Metal bindingi281CalciumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • regulation of body fluid levels Source: MGI
  • renal inner medulla development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Calcium, Chloride, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride channel protein ClC-Ka
Short name:
Chloride channel Ka
Alternative name(s):
ClC-K1
Gene namesi
Name:Clcnka
Synonyms:Clcnk1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1329026. Clcnka.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei52 – 72HelicalSequence analysisAdd BLAST21
Transmembranei94 – 114HelicalSequence analysisAdd BLAST21
Transmembranei161 – 181HelicalSequence analysisAdd BLAST21
Transmembranei204 – 224HelicalSequence analysisAdd BLAST21
Transmembranei236 – 256HelicalSequence analysisAdd BLAST21
Transmembranei282 – 302HelicalSequence analysisAdd BLAST21
Transmembranei325 – 345HelicalSequence analysisAdd BLAST21
Transmembranei396 – 416HelicalSequence analysisAdd BLAST21
Transmembranei417 – 437HelicalSequence analysisAdd BLAST21
Transmembranei458 – 478HelicalSequence analysisAdd BLAST21
Transmembranei486 – 506HelicalSequence analysisAdd BLAST21
Topological domaini507 – 687CytoplasmicSequence analysisAdd BLAST181

GO - Cellular componenti

  • basolateral plasma membrane Source: MGI
  • chloride channel complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000944561 – 687Chloride channel protein ClC-KaAdd BLAST687

Proteomic databases

PaxDbiQ9WUB7.
PRIDEiQ9WUB7.

PTM databases

PhosphoSitePlusiQ9WUB7.

Expressioni

Tissue specificityi

Specifically expressed in the kidney. All nephron segments expressing BSND also express CLCNK proreins.2 Publications

Gene expression databases

BgeeiENSMUSG00000033770.
CleanExiMM_CLCNKA.
ExpressionAtlasiQ9WUB7. baseline and differential.
GenevisibleiQ9WUB7. MM.

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with BSND. Forms heteromers with BSND in the thin ascending limb of Henle.By similarity1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048520.

Structurei

3D structure databases

ProteinModelPortaliQ9WUB7.
SMRiQ9WUB7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini551 – 609CBS 1PROSITE-ProRule annotationAdd BLAST59
Domaini626 – 687CBS 2PROSITE-ProRule annotationAdd BLAST62

Sequence similaritiesi

Contains 2 CBS domains.PROSITE-ProRule annotation

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0476. Eukaryota.
COG0038. LUCA.
GeneTreeiENSGT00760000119109.
HOGENOMiHOG000231297.
HOVERGENiHBG005332.
InParanoidiQ9WUB7.
KOiK05017.
OMAiPCPHIRR.
OrthoDBiEOG091G01RJ.
TreeFamiTF300522.

Family and domain databases

Gene3Di1.10.3080.10. 2 hits.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002250. Cl_channel-K.
[Graphical view]
PfamiPF00571. CBS. 1 hit.
PF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01119. CLCHANNELKDY.
SMARTiSM00116. CBS. 1 hit.
[Graphical view]
SUPFAMiSSF81340. SSF81340. 2 hits.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WUB7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEELVGLREG SSGKPVTLQE LWGPCPRIRR GIRGGLEWLK ERLFRAREDW
60 70 80 90 100
YFLVALGVLM ALISYAMNFA IGRVVRAHKW LYREVGDGHL LRYLSWTVYP
110 120 130 140 150
VALLSFSSGF SQSITPFSGG SGLPELKTML SGVVLENYLD IKNFGAKVVG
160 170 180 190 200
LSCTLATGST IFLGKVGPFV HLSVMISAYL GRVRAKAVGD TESKAKEVEM
210 220 230 240 250
LSAAAAVGVA TVFAAPFSGV LFSIEVMSSH FSVWNYWRGF FAATCGAFMF
260 270 280 290 300
RLLAVFNSEQ ETITSIYKTR FRVDVPFDLP EIFFFVALGF ICGILSCVYL
310 320 330 340 350
FCQRNFLRFI KTNRYTSKLL ATSKPSYAAL VALVLASVTY PPGVGRFMAS
360 370 380 390 400
RLSMAEHLHS LFDNNSWALM TRNASPPWPS EPDPQNLWFE WYHPRFTIFG
410 420 430 440 450
TLAFFLIMKF WMLILATTIP MPAGYFMPIF IIGAAIGRLL GEALSVAFPE
460 470 480 490 500
GIVAGGEVYP IMPGGYALAG AAAFSGAVTH TISTALLAFE LTGQIVHALP
510 520 530 540 550
VLMAVLAANV ISQNLQPSFY DGTIMAKKLP YLPWIRGRQI GSYPVTVEHF
560 570 580 590 600
MNSNLTTLAK DMPLEEVVKV VTSTDVSQYP LVETRESQTL VGIVERTHLV
610 620 630 640 650
QALQTQPASW APGQERFLQD ILAGGCPTQP VTLQLSPETS LYQAHRLFEL
660 670 680
LTLQALFVTS RGRAVGSVSW VELKKAISTL INPPAPK
Length:687
Mass (Da):75,684
Last modified:July 27, 2011 - v2
Checksum:iA04ADFFC220224FA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti372R → W in AAH37077 (PubMed:15489334).Curated1
Sequence conflicti380S → A in AAD21083 (PubMed:11014860).Curated1
Sequence conflicti380S → A in AAH37077 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124848 mRNA. Translation: AAD21083.1.
AY373832 mRNA. Translation: AAQ81628.1.
AL670285 Genomic DNA. Translation: CAM20395.1.
BC037077 mRNA. Translation: AAH37077.1.
CCDSiCCDS18871.1.
RefSeqiNP_001139779.1. NM_001146307.1.
NP_077723.3. NM_024412.3.
UniGeneiMm.167900.

Genome annotation databases

EnsembliENSMUST00000042617; ENSMUSP00000048520; ENSMUSG00000033770.
ENSMUST00000105790; ENSMUSP00000101416; ENSMUSG00000033770.
GeneIDi12733.
KEGGimmu:12733.
UCSCiuc008voe.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124848 mRNA. Translation: AAD21083.1.
AY373832 mRNA. Translation: AAQ81628.1.
AL670285 Genomic DNA. Translation: CAM20395.1.
BC037077 mRNA. Translation: AAH37077.1.
CCDSiCCDS18871.1.
RefSeqiNP_001139779.1. NM_001146307.1.
NP_077723.3. NM_024412.3.
UniGeneiMm.167900.

3D structure databases

ProteinModelPortaliQ9WUB7.
SMRiQ9WUB7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048520.

PTM databases

PhosphoSitePlusiQ9WUB7.

Proteomic databases

PaxDbiQ9WUB7.
PRIDEiQ9WUB7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000042617; ENSMUSP00000048520; ENSMUSG00000033770.
ENSMUST00000105790; ENSMUSP00000101416; ENSMUSG00000033770.
GeneIDi12733.
KEGGimmu:12733.
UCSCiuc008voe.2. mouse.

Organism-specific databases

CTDi1187.
MGIiMGI:1329026. Clcnka.

Phylogenomic databases

eggNOGiKOG0476. Eukaryota.
COG0038. LUCA.
GeneTreeiENSGT00760000119109.
HOGENOMiHOG000231297.
HOVERGENiHBG005332.
InParanoidiQ9WUB7.
KOiK05017.
OMAiPCPHIRR.
OrthoDBiEOG091G01RJ.
TreeFamiTF300522.

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Miscellaneous databases

PROiQ9WUB7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033770.
CleanExiMM_CLCNKA.
ExpressionAtlasiQ9WUB7. baseline and differential.
GenevisibleiQ9WUB7. MM.

Family and domain databases

Gene3Di1.10.3080.10. 2 hits.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002250. Cl_channel-K.
[Graphical view]
PfamiPF00571. CBS. 1 hit.
PF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01119. CLCHANNELKDY.
SMARTiSM00116. CBS. 1 hit.
[Graphical view]
SUPFAMiSSF81340. SSF81340. 2 hits.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLCKA_MOUSE
AccessioniPrimary (citable) accession number: Q9WUB7
Secondary accession number(s): A2ADB3, Q6UB69, Q8JZU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.