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Protein

Chloride channel protein ClC-Kb

Gene

Clcnkb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. May be important in urinary concentrating mechanisms. May be the basolateral chloride channel mediating net chloride absorption in CTAL cells.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi259CalciumBy similarity1
Metal bindingi261CalciumBy similarity1
Metal bindingi278CalciumBy similarity1
Metal bindingi281CalciumBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Calcium, Chloride, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride channel protein ClC-Kb
Short name:
Chloride channel Kb
Alternative name(s):
ClC-K2
Gene namesi
Name:Clcnkb
Synonyms:Clckb, Clcnk1l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1930643. Clcnkb.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 51CytoplasmicSequence analysisAdd BLAST51
Transmembranei52 – 72HelicalSequence analysisAdd BLAST21
Transmembranei90 – 110HelicalSequence analysisAdd BLAST21
Transmembranei161 – 181HelicalSequence analysisAdd BLAST21
Transmembranei202 – 222HelicalSequence analysisAdd BLAST21
Transmembranei236 – 256HelicalSequence analysisAdd BLAST21
Transmembranei275 – 295HelicalSequence analysisAdd BLAST21
Transmembranei329 – 349HelicalSequence analysisAdd BLAST21
Transmembranei396 – 416HelicalSequence analysisAdd BLAST21
Transmembranei417 – 437HelicalSequence analysisAdd BLAST21
Transmembranei458 – 478HelicalSequence analysisAdd BLAST21
Transmembranei486 – 506HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000944601 – 687Chloride channel protein ClC-KbAdd BLAST687

Proteomic databases

PaxDbiQ9WUB6.
PRIDEiQ9WUB6.

PTM databases

iPTMnetiQ9WUB6.
PhosphoSitePlusiQ9WUB6.

Expressioni

Tissue specificityi

Specifically expressed in the kidney. All nephron segments expressing BSND also express CLCNK proreins.2 Publications

Gene expression databases

BgeeiENSMUSG00000006216.
CleanExiMM_CLCNKB.
ExpressionAtlasiQ9WUB6. baseline and differential.
GenevisibleiQ9WUB6. MM.

Interactioni

Subunit structurei

Interacts with BSND. Forms heteromers with BSND in the thick ascending limb of Henle and more distal segments.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000006378.

Structurei

3D structure databases

ProteinModelPortaliQ9WUB6.
SMRiQ9WUB6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini551 – 609CBS 1PROSITE-ProRule annotationAdd BLAST59
Domaini626 – 687CBS 2PROSITE-ProRule annotationAdd BLAST62

Sequence similaritiesi

Contains 2 CBS domains.PROSITE-ProRule annotation

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0476. Eukaryota.
COG0038. LUCA.
GeneTreeiENSGT00760000119109.
HOGENOMiHOG000231297.
HOVERGENiHBG005332.
InParanoidiQ9WUB6.
KOiK05018.
OMAiHETHNLF.
OrthoDBiEOG091G01RJ.
TreeFamiTF300522.

Family and domain databases

Gene3Di1.10.3080.10. 2 hits.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002250. Cl_channel-K.
[Graphical view]
PfamiPF00571. CBS. 1 hit.
PF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01119. CLCHANNELKDY.
SUPFAMiSSF81340. SSF81340. 2 hits.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9WUB6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEELVGLREG ASKKPVPLQE LWGPCPRIRR NIQGGLEWLK ERLFRVGEDW
60 70 80 90 100
YFLVALGVLM ALISYAMNFT IGRVVRAHKW LYREIGDGHL LRYLSWTVYP
110 120 130 140 150
VALLSFSSGF SQSITPSSGG SGIPEVKTIL TGVVLEDYLD IKNFGAKVVG
160 170 180 190 200
LSCTLATGST IFLGKLGPFV HLSVMIAAYL GRVRTKTVGE PESKTKEMEL
210 220 230 240 250
LAAGAAVGVA TVFAAPISGV LFSIEVMSSH FSVWDYWRGF FAATCGAFMF
260 270 280 290 300
HLLAVFNNEQ ETITSIYKTS FPVDIPFDLP EIFFFVALGA ICGILSCGYN
310 320 330 340 350
YSQRTFLFFL KANGFTSKLL ATSKPLYSAL AAVVLASITY PPGVGHFMAS
360 370 380 390 400
RLSMSEHLET LFDNNSWALM TKNSSPPWAA EPDPQKLWLE WCHPQLTVFG
410 420 430 440 450
TLVFFLVMKF WMLILATTIP IPAGYFLPIF IYGAVIGRLF GEVLSVAFPE
460 470 480 490 500
GIVAGGRVNP IMPGAYALAG AAAFSGAVTH TLSTALLAFE VTGQLVHALP
510 520 530 540 550
VLMAVLAANA ISQSFQPSFY DGTIIVKKLP YLPWIRGRQI GSHSVTVGHF
560 570 580 590 600
MNCALTTLAK DMPLEQVIQV VISTDVTQYP LVETTESQTL VGVVKRTHLV
610 620 630 640 650
QALQTEPASW APGQQPCLQD ILANGCPTQP VTLQLSLETS LHETHNLFEL
660 670 680
LNLQTLFVTS RGRAVGSVSW VELKKAISTL TNPPAPK
Length:687
Mass (Da):75,009
Last modified:July 27, 2011 - v2
Checksum:iBEF5A605DB3A3025
GO
Isoform 2 (identifier: Q9WUB6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     673-676: LKKA → VLGS
     677-687: Missing.

Note: Due to intron retention. No experimental confirmation available.
Show »
Length:676
Mass (Da):73,804
Checksum:i9C2B2351928BC5E5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti92R → L in AAD21082 (PubMed:11014860).Curated1
Sequence conflicti92R → L in AAH19983 (PubMed:15489334).Curated1
Sequence conflicti431I → V in AAD21082 (PubMed:11014860).Curated1
Sequence conflicti431I → V in AAH19983 (PubMed:15489334).Curated1
Sequence conflicti554A → T in AAD21082 (PubMed:11014860).Curated1
Sequence conflicti554A → T in AAH19983 (PubMed:15489334).Curated1
Sequence conflicti571V → M in AAD21082 (PubMed:11014860).Curated1
Sequence conflicti571V → M in AAH19983 (PubMed:15489334).Curated1
Sequence conflicti617C → Y in AAH19983 (PubMed:15489334).Curated1
Sequence conflicti637L → P in AAD21082 (PubMed:11014860).Curated1
Sequence conflicti637L → P in AAH19983 (PubMed:15489334).Curated1
Sequence conflicti667S → F in AAD21082 (PubMed:11014860).Curated1
Sequence conflicti667S → F in AAH19983 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_011757673 – 676LKKA → VLGS in isoform 2. 1 Publication4
Alternative sequenceiVSP_011758677 – 687Missing in isoform 2. 1 PublicationAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124847 mRNA. Translation: AAD21082.1.
AL670285 Genomic DNA. Translation: CAM20398.1.
CH466615 Genomic DNA. Translation: EDL13372.1.
BC019983 mRNA. Translation: AAH19983.1.
CCDSiCCDS18872.1. [Q9WUB6-1]
RefSeqiNP_062675.2. NM_019701.2. [Q9WUB6-1]
XP_011248609.1. XM_011250307.1. [Q9WUB6-1]
UniGeneiMm.24882.

Genome annotation databases

EnsembliENSMUST00000006378; ENSMUSP00000006378; ENSMUSG00000006216. [Q9WUB6-1]
ENSMUST00000105788; ENSMUSP00000101414; ENSMUSG00000006216. [Q9WUB6-2]
GeneIDi56365.
KEGGimmu:56365.
UCSCiuc008voh.2. mouse. [Q9WUB6-1]
uc008voi.2. mouse. [Q9WUB6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124847 mRNA. Translation: AAD21082.1.
AL670285 Genomic DNA. Translation: CAM20398.1.
CH466615 Genomic DNA. Translation: EDL13372.1.
BC019983 mRNA. Translation: AAH19983.1.
CCDSiCCDS18872.1. [Q9WUB6-1]
RefSeqiNP_062675.2. NM_019701.2. [Q9WUB6-1]
XP_011248609.1. XM_011250307.1. [Q9WUB6-1]
UniGeneiMm.24882.

3D structure databases

ProteinModelPortaliQ9WUB6.
SMRiQ9WUB6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000006378.

PTM databases

iPTMnetiQ9WUB6.
PhosphoSitePlusiQ9WUB6.

Proteomic databases

PaxDbiQ9WUB6.
PRIDEiQ9WUB6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000006378; ENSMUSP00000006378; ENSMUSG00000006216. [Q9WUB6-1]
ENSMUST00000105788; ENSMUSP00000101414; ENSMUSG00000006216. [Q9WUB6-2]
GeneIDi56365.
KEGGimmu:56365.
UCSCiuc008voh.2. mouse. [Q9WUB6-1]
uc008voi.2. mouse. [Q9WUB6-2]

Organism-specific databases

CTDi1188.
MGIiMGI:1930643. Clcnkb.

Phylogenomic databases

eggNOGiKOG0476. Eukaryota.
COG0038. LUCA.
GeneTreeiENSGT00760000119109.
HOGENOMiHOG000231297.
HOVERGENiHBG005332.
InParanoidiQ9WUB6.
KOiK05018.
OMAiHETHNLF.
OrthoDBiEOG091G01RJ.
TreeFamiTF300522.

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Miscellaneous databases

PROiQ9WUB6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000006216.
CleanExiMM_CLCNKB.
ExpressionAtlasiQ9WUB6. baseline and differential.
GenevisibleiQ9WUB6. MM.

Family and domain databases

Gene3Di1.10.3080.10. 2 hits.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002250. Cl_channel-K.
[Graphical view]
PfamiPF00571. CBS. 1 hit.
PF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01119. CLCHANNELKDY.
SUPFAMiSSF81340. SSF81340. 2 hits.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLCKB_MOUSE
AccessioniPrimary (citable) accession number: Q9WUB6
Secondary accession number(s): A2ADB6, Q8VCF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.