Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glycogen phosphorylase, muscle form

Gene

Pygm

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.

Catalytic activityi

((1->4)-alpha-D-glucosyl)(n) + phosphate = ((1->4)-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei76 – 761AMPBy similarity
Sitei109 – 1091Involved in the association of subunitsBy similarity
Sitei143 – 1431Involved in the association of subunitsBy similarity
Sitei156 – 1561May be involved in allosteric controlBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen metabolism

Keywords - Ligandi

Nucleotide-binding, Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-MMU-70221. Glycogen breakdown (glycogenolysis).

Protein family/group databases

CAZyiGT35. Glycosyltransferase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogen phosphorylase, muscle form (EC:2.4.1.1)
Alternative name(s):
Myophosphorylase
Gene namesi
Name:Pygm
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:97830. Pygm.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 842841Glycogen phosphorylase, muscle formPRO_0000188531Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei15 – 151Phosphoserine; by PHK; in form phosphorylase ABy similarity
Modified residuei204 – 2041PhosphotyrosineBy similarity
Modified residuei227 – 2271PhosphotyrosineBy similarity
Modified residuei364 – 3641N6-succinyllysineBy similarity
Modified residuei430 – 4301PhosphoserineCombined sources
Modified residuei473 – 4731PhosphotyrosineCombined sources
Modified residuei514 – 5141PhosphoserineBy similarity
Modified residuei524 – 5241PhosphoserineBy similarity
Modified residuei681 – 6811N6-(pyridoxal phosphate)lysineBy similarity
Modified residuei747 – 7471PhosphoserineBy similarity
Modified residuei748 – 7481PhosphoserineBy similarity

Post-translational modificationi

Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9WUB3.
MaxQBiQ9WUB3.
PaxDbiQ9WUB3.
PRIDEiQ9WUB3.

PTM databases

iPTMnetiQ9WUB3.
PhosphoSiteiQ9WUB3.
SwissPalmiQ9WUB3.

Expressioni

Gene expression databases

BgeeiQ9WUB3.
CleanExiMM_PYGM.
ExpressionAtlasiQ9WUB3. baseline and differential.
GenevisibleiQ9WUB3. MM.

Interactioni

Subunit structurei

Homodimer. Dimers associate into a tetramer to form the enzymatically active phosphorylase A (By similarity).By similarity

Protein-protein interaction databases

BioGridi202528. 1 interaction.
IntActiQ9WUB3. 5 interactions.
MINTiMINT-4107503.
STRINGi10090.ENSMUSP00000047564.

Structurei

3D structure databases

ProteinModelPortaliQ9WUB3.
SMRiQ9WUB3. Positions 14-842.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycogen phosphorylase family.Curated

Phylogenomic databases

eggNOGiKOG2099. Eukaryota.
COG0058. LUCA.
GeneTreeiENSGT00390000016886.
HOGENOMiHOG000278444.
HOVERGENiHBG006848.
InParanoidiQ9WUB3.
KOiK00688.
OMAiRSCAYTN.
OrthoDBiEOG7JQBMK.
PhylomeDBiQ9WUB3.
TreeFamiTF300309.

Family and domain databases

InterProiIPR011833. Glycg_phsphrylas.
IPR000811. Glyco_trans_35.
[Graphical view]
PANTHERiPTHR11468. PTHR11468. 1 hit.
PfamiPF00343. Phosphorylase. 1 hit.
[Graphical view]
PIRSFiPIRSF000460. Pprylas_GlgP. 1 hit.
TIGRFAMsiTIGR02093. P_ylase. 1 hit.
PROSITEiPS00102. PHOSPHORYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9WUB3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRPLSDQDK RKQISVRGLA GVENVSELKK NFNRHLHFTL VKDRNVATPR
60 70 80 90 100
DYYFALAHTV RDHLVGRWIR TQQHYYEKDP KRIYYLSLEF YMGRTLQNTM
110 120 130 140 150
VNLALENACD EATYQLGLDM EELEEIEEDA GLGNGGLGRL AACFLDSMAT
160 170 180 190 200
LGLAAYGYGI RYEFGIFNQK ICGGWQMEEA DDWLRYGNPW EKARPEFTLP
210 220 230 240 250
VHFYGRVEHT SQGAKWVDTQ VVLAMPYDTP VPGYRNNVVN TMRLWSAKAP
260 270 280 290 300
NDFNLKDFNV GGYIQAVLDR NLAENISRVL YPNDNFFEGK ELRLKQEYFV
310 320 330 340 350
VAATLQDIIR RFKSSKFGSR DPVRTNFDAF PDKVAIQLND THPSLAIPEL
360 370 380 390 400
MRILVDLERL DWDKAWDVTV KTCAYTNHTV LPEALERWPV HLMETLLPRH
410 420 430 440 450
LQIIYEINQR FLNRVAAAFP GDVDRLRRMS LVEEGAVKRI NMAHLCIAGS
460 470 480 490 500
HAVNGVARIH SEILKKTIFK DFYELEPHKF QNKTNGITPR RWLVLCNPGL
510 520 530 540 550
AEVIAERIGE DYISDLDQLR KLLSYVDDEA FIRDVAKVKQ ENKLKFSAYL
560 570 580 590 600
EREYKVHINP NSLFDVQVKR IHEYKRQLLN CLHIITLYNR IKREPNRFMV
610 620 630 640 650
PRTIMIGGKA APGYHMAKMI IKLITAIGDV VNHDPAVGDR LRVIFLENYR
660 670 680 690 700
VSLAEKVIPA ADLSEQISTA GTEASGTGNM KFMLNGALTI GTMDGANVEM
710 720 730 740 750
AEEAGEENFF IFGMRVEDVE RLDQRGYNAQ EYYDRIPELR QIIEQLSSGF
760 770 780 790 800
FSPKQPDLFK DIVNMLMHHD RFKVFADYEE YIKCQDKVSE LYKNPREWTR
810 820 830 840
MVIRNIATSG KFSSDRTIAQ YAREIWGVEP SRQRLPAPDE KI
Length:842
Mass (Da):97,286
Last modified:January 23, 2007 - v3
Checksum:iB55CC8EFF517DCD9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124787 mRNA. Translation: AAD30476.1.
BC012961 mRNA. Translation: AAH12961.1.
CCDSiCCDS29504.1.
RefSeqiNP_035354.1. NM_011224.2.
UniGeneiMm.27806.

Genome annotation databases

EnsembliENSMUST00000035269; ENSMUSP00000047564; ENSMUSG00000032648.
ENSMUST00000201001; ENSMUSP00000143844; ENSMUSG00000106877.
GeneIDi19309.
KEGGimmu:19309.
UCSCiuc008gio.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124787 mRNA. Translation: AAD30476.1.
BC012961 mRNA. Translation: AAH12961.1.
CCDSiCCDS29504.1.
RefSeqiNP_035354.1. NM_011224.2.
UniGeneiMm.27806.

3D structure databases

ProteinModelPortaliQ9WUB3.
SMRiQ9WUB3. Positions 14-842.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202528. 1 interaction.
IntActiQ9WUB3. 5 interactions.
MINTiMINT-4107503.
STRINGi10090.ENSMUSP00000047564.

Protein family/group databases

CAZyiGT35. Glycosyltransferase Family 35.

PTM databases

iPTMnetiQ9WUB3.
PhosphoSiteiQ9WUB3.
SwissPalmiQ9WUB3.

Proteomic databases

EPDiQ9WUB3.
MaxQBiQ9WUB3.
PaxDbiQ9WUB3.
PRIDEiQ9WUB3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035269; ENSMUSP00000047564; ENSMUSG00000032648.
ENSMUST00000201001; ENSMUSP00000143844; ENSMUSG00000106877.
GeneIDi19309.
KEGGimmu:19309.
UCSCiuc008gio.1. mouse.

Organism-specific databases

CTDi5837.
MGIiMGI:97830. Pygm.

Phylogenomic databases

eggNOGiKOG2099. Eukaryota.
COG0058. LUCA.
GeneTreeiENSGT00390000016886.
HOGENOMiHOG000278444.
HOVERGENiHBG006848.
InParanoidiQ9WUB3.
KOiK00688.
OMAiRSCAYTN.
OrthoDBiEOG7JQBMK.
PhylomeDBiQ9WUB3.
TreeFamiTF300309.

Enzyme and pathway databases

ReactomeiR-MMU-70221. Glycogen breakdown (glycogenolysis).

Miscellaneous databases

PROiQ9WUB3.
SOURCEiSearch...

Gene expression databases

BgeeiQ9WUB3.
CleanExiMM_PYGM.
ExpressionAtlasiQ9WUB3. baseline and differential.
GenevisibleiQ9WUB3. MM.

Family and domain databases

InterProiIPR011833. Glycg_phsphrylas.
IPR000811. Glyco_trans_35.
[Graphical view]
PANTHERiPTHR11468. PTHR11468. 1 hit.
PfamiPF00343. Phosphorylase. 1 hit.
[Graphical view]
PIRSFiPIRSF000460. Pprylas_GlgP. 1 hit.
TIGRFAMsiTIGR02093. P_ylase. 1 hit.
PROSITEiPS00102. PHOSPHORYLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Up-regulation of nuclear and mitochondrial genes in the skeletal muscle of mice lacking the heart/muscle isoform of the adenine nucleotide translocator."
    Murdock D.G., Boone B.E., Esposito L.A., Wallace D.C.
    J. Biol. Chem. 274:14429-14433(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
    Tissue: Skeletal muscle.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary gland.
  3. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-473, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-430, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Lung, Spleen and Testis.

Entry informationi

Entry nameiPYGM_MOUSE
AccessioniPrimary (citable) accession number: Q9WUB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 137 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.