Reviewed, UniProtKB/Swiss-Prot Q9WUB3 (PYGM_MOUSE)

Names and origin

Protein names

Recommended name:
    Glycogen phosphorylase, muscle form
    EC=2.4.1.1
Alternative name(s):
    Myophosphorylase

Gene names

Name:

Pygm

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	842 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
Catalytic activity	(1,4-alpha-D-glucosyl)(n) + phosphate = (1,4-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate.
Cofactor	Pyridoxal phosphate.
Subunit structure	Homodimer. Dimers associate into a tetramer to form the enzymatically active phosphorylase A By similarity.
Post-translational modification	Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A By similarity.
Sequence similarities	Belongs to the glycogen phosphorylase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen metabolism
Ligand	Nucleotide-binding Pyridoxal phosphate
Molecular function	Glycosyltransferase Transferase
PTM	Acetylation Phosphoprotein
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological process	glycogen catabolic process Inferred from direct assay. Source: MGI
Molecular function	glycogen phosphorylase activity Inferred from direct assay. Source: MGI nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 842	841	Glycogen phosphorylase, muscle form		PRO_0000188531
Sites
	Binding site	76	1	AMP By similarity
	Site	109	1	Involved in the association of subunits By similarity
	Site	143	1	Involved in the association of subunits By similarity
	Site	156	1	May be involved in allosteric control By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	15	1	Phosphoserine; by PHK; in form phosphorylase A By similarity
	Modified residue	316	1	N6-acetyllysine By similarity
	Modified residue	473	1	Phosphotyrosine Ref.3
	Modified residue	681	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9WUB3-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: B55CC8EFF517DCD9	FASTA	842	97,286
	10 20 30 40 50 60 MSRPLSDQDK RKQISVRGLA GVENVSELKK NFNRHLHFTL VKDRNVATPR DYYFALAHTV 70 80 90 100 110 120 RDHLVGRWIR TQQHYYEKDP KRIYYLSLEF YMGRTLQNTM VNLALENACD EATYQLGLDM 130 140 150 160 170 180 EELEEIEEDA GLGNGGLGRL AACFLDSMAT LGLAAYGYGI RYEFGIFNQK ICGGWQMEEA 190 200 210 220 230 240 DDWLRYGNPW EKARPEFTLP VHFYGRVEHT SQGAKWVDTQ VVLAMPYDTP VPGYRNNVVN 250 260 270 280 290 300 TMRLWSAKAP NDFNLKDFNV GGYIQAVLDR NLAENISRVL YPNDNFFEGK ELRLKQEYFV 310 320 330 340 350 360 VAATLQDIIR RFKSSKFGSR DPVRTNFDAF PDKVAIQLND THPSLAIPEL MRILVDLERL 370 380 390 400 410 420 DWDKAWDVTV KTCAYTNHTV LPEALERWPV HLMETLLPRH LQIIYEINQR FLNRVAAAFP 430 440 450 460 470 480 GDVDRLRRMS LVEEGAVKRI NMAHLCIAGS HAVNGVARIH SEILKKTIFK DFYELEPHKF 490 500 510 520 530 540 QNKTNGITPR RWLVLCNPGL AEVIAERIGE DYISDLDQLR KLLSYVDDEA FIRDVAKVKQ 550 560 570 580 590 600 ENKLKFSAYL EREYKVHINP NSLFDVQVKR IHEYKRQLLN CLHIITLYNR IKREPNRFMV 610 620 630 640 650 660 PRTIMIGGKA APGYHMAKMI IKLITAIGDV VNHDPAVGDR LRVIFLENYR VSLAEKVIPA 670 680 690 700 710 720 ADLSEQISTA GTEASGTGNM KFMLNGALTI GTMDGANVEM AEEAGEENFF IFGMRVEDVE 730 740 750 760 770 780 RLDQRGYNAQ EYYDRIPELR QIIEQLSSGF FSPKQPDLFK DIVNMLMHHD RFKVFADYEE 790 800 810 820 830 840 YIKCQDKVSE LYKNPREWTR MVIRNIATSG KFSSDRTIAQ YAREIWGVEP SRQRLPAPDE KI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Up-regulation of nuclear and mitochondrial genes in the skeletal muscle of mice lacking the heart/muscle isoform of the adenine nucleotide translocator." Murdock D.G., Boone B.E., Esposito L.A., Wallace D.C. J. Biol. Chem. 274:14429-14433(1999) [PubMed: 10318868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Skeletal muscle.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. Tissue: Mammary gland.
[3]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed: 18034455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-473, MASS SPECTROMETRY. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF124787 mRNA. Translation: AAD30476.1. BC012961 mRNA. Translation: AAH12961.1.
IPI	IPI00225275.
RefSeq	NP_035354.1.
UniGene	Mm.27806
3D structure databases
HSSP	HSSP built from PDB template 1K08 based on UniProtKB P00489.
SMR	Q9WUB3. Positions 13-836.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9WUB3.
Protein family/group databases
CAZy	GT35. Glycosyltransferase Family 35.
PTM databases
PhosphoSite	Q9WUB3.
Proteomic databases
PRIDE	Q9WUB3.
Genome annotation databases
Ensembl	ENSMUST00000035269; ENSMUSP00000047564; ENSMUSG00000032648; Mus musculus. [Genome view]
GeneID	19309.
KEGG	mmu:19309.
UCSC	uc008gio.1. mouse.
Organism-specific databases
CTD	19309.
MGI	MGI:97830. Pygm.
Phylogenomic databases
HOGENOM	Q9WUB3.
HOVERGEN	Q9WUB3.
OMA	NQKISGG.
Enzyme and pathway databases
BRENDA	2.4.1.1. 244.
Gene expression databases
ArrayExpress	Q9WUB3.
Bgee	Q9WUB3.
CleanEx	MM_PYGM.
Genevestigator	Q9WUB3.
GermOnline	ENSMUSG00000032648. Mus musculus.
Family and domain databases
InterPro	IPR011833. Glycg_phsphrylas. IPR000811. Glyco_trans_35. [Graphical view]
PANTHER	PTHR11468. Glyco_trans_35. 1 hit.
Pfam	PF00343. Phosphorylase. 1 hit. [Graphical view]
PIRSF	PIRSF000460. Pprylas_GlgP. 1 hit.
TIGRFAMs	TIGR02093. P_ylase. 1 hit.
PROSITE	PS00102. PHOSPHORYLASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	296275.
SOURCE	Search...

Entry information

Entry name

PYGM_MOUSE

Accession

Primary (citable) accession number: Q9WUB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2001
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 81 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)