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Protein

ATP-dependent 6-phosphofructokinase, platelet type

Gene

Pfkp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (Gpi), Glucose-6-phosphate isomerase (Gpi1), Glucose-6-phosphate isomerase (Gpi1), Glucose-6-phosphate isomerase (Gpi1)
  3. ATP-dependent 6-phosphofructokinase, liver type (Pfkl), ATP-dependent 6-phosphofructokinase (Pfkp), ATP-dependent 6-phosphofructokinase (Pfkm), ATP-dependent 6-phosphofructokinase, platelet type (Pfkp), ATP-dependent 6-phosphofructokinase, muscle type (Pfkm)
  4. Fructose-bisphosphate aldolase (Aldoart1), Fructose-bisphosphate aldolase (Aldoa), Fructose-bisphosphate aldolase (Aldoa), Fructose-bisphosphate aldolase (Aldoart2), Fructose-bisphosphate aldolase (Aldoart1), Fructose-bisphosphate aldolase (Aldoa), Fructose-bisphosphate aldolase (Aldoa), Fructose-bisphosphate aldolase (Aldob), Fructose-bisphosphate aldolase C (Aldoc), Fructose-bisphosphate aldolase A (Aldoa), Fructose-bisphosphate aldolase B (Aldob), Fructose-bisphosphate aldolase (Aldob)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33ATP; via amide nitrogenUniRule annotation1
Metal bindingi127Magnesium; catalyticUniRule annotation1
Active sitei174Proton acceptorUniRule annotation1
Binding sitei209Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei272SubstrateUniRule annotation1
Binding sitei300Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei480Allosteric activator fructose 2,6-bisphosphateUniRule annotation1
Binding sitei575Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei638Allosteric activator fructose 2,6-bisphosphateUniRule annotation1
Binding sitei664Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation1
Binding sitei743Allosteric activator fructose 2,6-bisphosphateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi96 – 97ATPUniRule annotation2
Nucleotide bindingi126 – 129ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.11. 3474.
ReactomeiR-MMU-70171. Glycolysis.
SABIO-RKQ9WUA3.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase, platelet typeUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PFK-P
Alternative name(s):
6-phosphofructokinase type C
Phosphofructo-1-kinase isozyme C
Short name:
PFK-C
PhosphohexokinaseUniRule annotation
Gene namesi
Name:Pfkp
Synonyms:Pfkc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1891833. Pfkp.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • 6-phosphofructokinase complex Source: MGI
  • cell-cell adherens junction Source: MGI
  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • extracellular exosome Source: MGI
  • extracellular matrix Source: MGI
  • membrane Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001120251 – 784ATP-dependent 6-phosphofructokinase, platelet typeAdd BLAST784

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2PhosphoserineBy similarity1
Modified residuei6PhosphoserineBy similarity1
Modified residuei20PhosphoserineBy similarity1
Modified residuei141PhosphoserineBy similarity1
Modified residuei394N6-acetyllysineBy similarity1
Modified residuei485N6-acetyllysineBy similarity1
Glycosylationi539O-linked (GlcNAc)By similarity1
Modified residuei650PhosphotyrosineCombined sources1
Modified residuei687N6-acetyllysineCombined sources1

Post-translational modificationi

GlcNAcylation decreases enzyme activity.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ9WUA3.
PaxDbiQ9WUA3.
PeptideAtlasiQ9WUA3.
PRIDEiQ9WUA3.

PTM databases

iPTMnetiQ9WUA3.
PhosphoSitePlusiQ9WUA3.
SwissPalmiQ9WUA3.

Expressioni

Tissue specificityi

Expression is constant during tumor growth and markedly decreases when cell proliferation stops.1 Publication

Gene expression databases

BgeeiENSMUSG00000021196.
CleanExiMM_PFKP.
ExpressionAtlasiQ9WUA3. baseline and differential.
GenevisibleiQ9WUA3. MM.

Interactioni

Subunit structurei

Homo- and heterotetramers.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207967. 3 interactors.
IntActiQ9WUA3. 5 interactors.
MINTiMINT-1676159.
STRINGi10090.ENSMUSP00000117030.

Structurei

3D structure databases

ProteinModelPortaliQ9WUA3.
SMRiQ9WUA3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 398N-terminal catalytic PFK domain 1Add BLAST398
Regioni172 – 174Substrate bindingUniRule annotation3
Regioni216 – 218Substrate bindingUniRule annotation3
Regioni306 – 309Substrate bindingUniRule annotation4
Regioni399 – 410Interdomain linkerAdd BLAST12
Regioni411 – 784C-terminal regulatory PFK domain 2Add BLAST374
Regioni537 – 541Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation5
Regioni582 – 584Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation3
Regioni670 – 673Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2440. Eukaryota.
COG0205. LUCA.
GeneTreeiENSGT00390000013209.
HOVERGENiHBG000976.
InParanoidiQ9WUA3.
KOiK00850.
OMAiKMCQKLS.
OrthoDBiEOG091G01YN.
TreeFamiTF300411.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E. 1 hit.
InterProiIPR009161. 6-Pfructokinase_euk.
IPR022953. ATP_PFK.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9WUA3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDLDSSSSS AYPKYLEHLS GDGKAIGVLT SGGDAQGMNA AVRAVVRMGI
60 70 80 90 100
YTGAKVYFIY EGYQGLVDGG SNIVEAKWDC VSSILQVGGT IIGSARCKAF
110 120 130 140 150
RSREGRLKAA CNLARLGITN LCVIGGDGSL TGANLFRKEW SGLLEELARN
160 170 180 190 200
GDIDNDTVQK YSYLNVVGMV GSIDNDFCGT DMTIGTDSAL HRIIEVVDAI
210 220 230 240 250
MTTAQSHQRT FVLEVMGRHC GYLALVSALT CGADWVFLPE SPPEEDWEEN
260 270 280 290 300
MCLKLSENRA RKKRLNIIIV SEGAIDMQNK PITSEKIKEL VVKNLGFDTR
310 320 330 340 350
VTILGHVQRG GTPSAFDRIL ASRMGVEAVI ALLEATPETP ACVVSLRGNQ
360 370 380 390 400
AVRLPLMECV QMTQDVQKAM DERRFKEAVK LRGRRFEGNL NTYKRLAIKL
410 420 430 440 450
PDEKIVKSNC NVAVINVGAP AAGMNAAVRS AVRVGIADGH KMFAIYDGFE
460 470 480 490 500
GFANGQIKEI GWADVGGWTG QGGSILGTKR TLPGKYLEKI AEQMHSHSIN
510 520 530 540 550
ALLIIGGFEA YLGLLELAAA REKHEAFCVP MVMVPATVSN NVPGSDFSIG
560 570 580 590 600
ADTALNTITD TCDRIKQSAS GTKRRVFIIE TMGGYCGYLA NMGALAAGAD
610 620 630 640 650
AAYIFEEPFD IGDLQSNVVH LTEKMKTSIQ RGLVLRNESC SVNYTTDFIY
660 670 680 690 700
QLYSEEGKGV FDCRKNVLGH MQQGGAPSPF DRNFGTKISA KAMEWISAKL
710 720 730 740 750
KGSQGTGKKF VSDDSICVLG ICKRDLLFQP VAELKKVTDF EHRIPKEQWW
760 770 780
LKLRPIMKIL AKYEASYDMS DSGKLESLQH HEEL
Length:784
Mass (Da):85,455
Last modified:November 1, 1999 - v1
Checksum:iE9C5AAABF26FCA65
GO
Isoform 2 (identifier: Q9WUA3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     457-496: IKEIGWADVG...LEKIAEQMHS → VSLPGVLGML...LVVGLYQILI
     497-784: Missing.

Note: May be due to intron retention. No experimental confirmation available.
Show »
Length:496
Mass (Da):53,682
Checksum:i22E3CB5080FD748C
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti104E → V in strain: NOD. 1 Publication1
Natural varianti145E → G in strain: C57BL/6J. 1 Publication1
Natural varianti180T → P in strain: C57BL/6. 1 Publication1
Natural varianti261R → Q in strain: LG/J and NOD. 2 Publications1
Natural varianti292V → M in strain: LG/J and NOD. 2 Publications1
Natural varianti402D → E in strain: C57BL/6J. 1 Publication1
Natural varianti679P → S in strain: C57BL/6J. 1 Publication1
Natural varianti694E → D in strain: LG/J and NOD. 2 Publications1
Natural varianti696I → F in strain: NOD. 1 Publication1
Natural varianti777S → P in strain: LG/J and NOD. 2 Publications1
Natural varianti782E → G in strain: LG/J and NOD. 2 Publications1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016664457 – 496IKEIG…EQMHS → VSLPGVLGMLKCYCIWGGHP PLTAPTKSRFLVVGLYQILI in isoform 2. 1 PublicationAdd BLAST40
Alternative sequenceiVSP_016665497 – 784Missing in isoform 2. 1 PublicationAdd BLAST288

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y19008 mRNA. Translation: CAB64347.1.
AF123533 mRNA. Translation: AAD23571.1.
AF249893
, AF250369, AF250370, AF250371, AF250372, AF251021 Genomic DNA. Translation: AAF75700.1.
AY779275 mRNA. Translation: AAX11357.1.
AY779276 mRNA. Translation: AAX11358.1.
AK049841 mRNA. Translation: BAC33949.1.
AK078254 mRNA. Translation: BAC37195.1.
AK152670 mRNA. Translation: BAE31405.1.
AK154125 mRNA. Translation: BAE32390.1.
AK165422 mRNA. Translation: BAE38177.1.
AK165425 mRNA. Translation: BAE38180.1.
AK170624 mRNA. Translation: BAE41918.1.
AK171062 mRNA. Translation: BAE42221.1.
CT010268 mRNA. Translation: CAJ18476.1.
BC006926 mRNA. Translation: AAH06926.1.
CCDSiCCDS26230.1. [Q9WUA3-1]
RefSeqiNP_001278000.1. NM_001291071.1.
NP_062677.1. NM_019703.4. [Q9WUA3-1]
UniGeneiMm.273874.

Genome annotation databases

EnsembliENSMUST00000138703; ENSMUSP00000117030; ENSMUSG00000021196. [Q9WUA3-1]
GeneIDi56421.
KEGGimmu:56421.
UCSCiuc007pjz.2. mouse. [Q9WUA3-1]
uc007pkc.2. mouse. [Q9WUA3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y19008 mRNA. Translation: CAB64347.1.
AF123533 mRNA. Translation: AAD23571.1.
AF249893
, AF250369, AF250370, AF250371, AF250372, AF251021 Genomic DNA. Translation: AAF75700.1.
AY779275 mRNA. Translation: AAX11357.1.
AY779276 mRNA. Translation: AAX11358.1.
AK049841 mRNA. Translation: BAC33949.1.
AK078254 mRNA. Translation: BAC37195.1.
AK152670 mRNA. Translation: BAE31405.1.
AK154125 mRNA. Translation: BAE32390.1.
AK165422 mRNA. Translation: BAE38177.1.
AK165425 mRNA. Translation: BAE38180.1.
AK170624 mRNA. Translation: BAE41918.1.
AK171062 mRNA. Translation: BAE42221.1.
CT010268 mRNA. Translation: CAJ18476.1.
BC006926 mRNA. Translation: AAH06926.1.
CCDSiCCDS26230.1. [Q9WUA3-1]
RefSeqiNP_001278000.1. NM_001291071.1.
NP_062677.1. NM_019703.4. [Q9WUA3-1]
UniGeneiMm.273874.

3D structure databases

ProteinModelPortaliQ9WUA3.
SMRiQ9WUA3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207967. 3 interactors.
IntActiQ9WUA3. 5 interactors.
MINTiMINT-1676159.
STRINGi10090.ENSMUSP00000117030.

PTM databases

iPTMnetiQ9WUA3.
PhosphoSitePlusiQ9WUA3.
SwissPalmiQ9WUA3.

Proteomic databases

EPDiQ9WUA3.
PaxDbiQ9WUA3.
PeptideAtlasiQ9WUA3.
PRIDEiQ9WUA3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000138703; ENSMUSP00000117030; ENSMUSG00000021196. [Q9WUA3-1]
GeneIDi56421.
KEGGimmu:56421.
UCSCiuc007pjz.2. mouse. [Q9WUA3-1]
uc007pkc.2. mouse. [Q9WUA3-2]

Organism-specific databases

CTDi5214.
MGIiMGI:1891833. Pfkp.

Phylogenomic databases

eggNOGiKOG2440. Eukaryota.
COG0205. LUCA.
GeneTreeiENSGT00390000013209.
HOVERGENiHBG000976.
InParanoidiQ9WUA3.
KOiK00850.
OMAiKMCQKLS.
OrthoDBiEOG091G01YN.
TreeFamiTF300411.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BRENDAi2.7.1.11. 3474.
ReactomeiR-MMU-70171. Glycolysis.
SABIO-RKQ9WUA3.

Miscellaneous databases

ChiTaRSiPfkp. mouse.
PROiQ9WUA3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021196.
CleanExiMM_PFKP.
ExpressionAtlasiQ9WUA3. baseline and differential.
GenevisibleiQ9WUA3. MM.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E. 1 hit.
InterProiIPR009161. 6-Pfructokinase_euk.
IPR022953. ATP_PFK.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPFKAP_MOUSE
AccessioniPrimary (citable) accession number: Q9WUA3
Secondary accession number(s): Q3TNA9
, Q3U4P1, Q3U7G4, Q4KUG1, Q543K8, Q8C5I6, Q9JI86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1999
Last modified: November 30, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.