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Protein

Phenylalanine--tRNA ligase beta subunit

Gene

Farsb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase beta subunit (EC:6.1.1.20)
Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunit
Short name:
PheRS
Gene namesi
Name:Farsb
Synonyms:Farsl, Farslb, Frsb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1346035. Farsb.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 589589Phenylalanine--tRNA ligase beta subunitPRO_0000127017Add
BLAST

Proteomic databases

EPDiQ9WUA2.
MaxQBiQ9WUA2.
PaxDbiQ9WUA2.
PRIDEiQ9WUA2.

PTM databases

iPTMnetiQ9WUA2.
PhosphoSiteiQ9WUA2.
SwissPalmiQ9WUA2.

Expressioni

Gene expression databases

BgeeiQ9WUA2.
CleanExiMM_FARSB.
ExpressionAtlasiQ9WUA2. baseline and differential.
GenevisibleiQ9WUA2. MM.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.By similarity

Protein-protein interaction databases

BioGridi204768. 1 interaction.
MINTiMINT-1865201.
STRINGi10090.ENSMUSP00000069508.

Structurei

3D structure databases

ProteinModelPortaliQ9WUA2.
SMRiQ9WUA2. Positions 1-589.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini302 – 37978B5PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 B5 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2472. Eukaryota.
COG0072. LUCA.
GeneTreeiENSGT00530000063489.
HOGENOMiHOG000105095.
HOVERGENiHBG009523.
InParanoidiQ9WUA2.
KOiK01890.
OMAiERPYVTG.
OrthoDBiEOG7R2BJ6.
TreeFamiTF105681.

Family and domain databases

Gene3Di3.30.56.20. 1 hit.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR004531. Phe-tRNA-synth_IIc_bsu_arc.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 2 hits.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR00471. pheT_arch. 1 hit.
PROSITEiPS51483. B5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WUA2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTVSVKRDL LFQALGRTYT DEEFDELCFE FGLELDEITS EKQIISKEQG
60 70 80 90 100
HGKAQGASDV VLYKIDVPAN RYDLLCLEGL ARGLQVFKER IKAPVYKRVM
110 120 130 140 150
PKGDIQKLVI TEETAKVRPF AVAAVLRNIK FTKDRYDSFI ELQEKLHQNI
160 170 180 190 200
CRKRALVAIG THDLDTLSGP FTYTAKRPSD IKFKPLNKTK EYTACELMNI
210 220 230 240 250
YKTDNHLKHY LHIIESKPLY PVIYDSNGVV LSMPPIINGN HSKITVNTRN
260 270 280 290 300
IFIECTGTDF TKAKIVLDII VTMFSEHCEN QFTVEAVEVV SPNGKSSTFP
310 320 330 340 350
ELPYRKEMVR ADLINKKVGI RETPANLAKL LTRMCLKSEV IGDGNQIEVE
360 370 380 390 400
IPPTRADVIH ACDIVEDAAI AYGYNNIQMT LPKTYTIANQ FPLNKLTELL
410 420 430 440 450
RLDMAAAGFT EALTFALCSQ EDIADKLGLD ISATKAVHIS NPKTAEFQVA
460 470 480 490 500
RTTLLPGLLK TIAANRKMPL PLKLFEISDV VVKDSGKDVG AKNYRHLCAV
510 520 530 540 550
YYNKTPGFEI IHGLLDRIMQ LLDVPPGEES GGYMIKASAG SAFFPGRCAE
560 570 580
IFVGGQSIGK LGVLHPDVIT KFELTMPCSS LEINIEPFL
Length:589
Mass (Da):65,697
Last modified:July 27, 2011 - v2
Checksum:i5EEE8FF0DF48E751
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti503 – 5031N → S in AAD26855 (PubMed:10375616).Curated
Sequence conflicti503 – 5031N → S in AAH16428 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF123263 mRNA. Translation: AAD26855.1.
AK010271 mRNA. Translation: BAB26810.1.
AK045432 mRNA. Translation: BAC32363.1.
BC016428 mRNA. Translation: AAH16428.1.
CCDSiCCDS15084.1.
RefSeqiNP_001265004.1. NM_001278075.1.
NP_035941.2. NM_011811.4.
UniGeneiMm.17898.
Mm.28922.
Mm.447792.

Genome annotation databases

EnsembliENSMUST00000068333; ENSMUSP00000069508; ENSMUSG00000026245.
ENSMUST00000170217; ENSMUSP00000129828; ENSMUSG00000026245.
GeneIDi23874.
KEGGimmu:23874.
UCSCiuc007bqf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF123263 mRNA. Translation: AAD26855.1.
AK010271 mRNA. Translation: BAB26810.1.
AK045432 mRNA. Translation: BAC32363.1.
BC016428 mRNA. Translation: AAH16428.1.
CCDSiCCDS15084.1.
RefSeqiNP_001265004.1. NM_001278075.1.
NP_035941.2. NM_011811.4.
UniGeneiMm.17898.
Mm.28922.
Mm.447792.

3D structure databases

ProteinModelPortaliQ9WUA2.
SMRiQ9WUA2. Positions 1-589.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204768. 1 interaction.
MINTiMINT-1865201.
STRINGi10090.ENSMUSP00000069508.

PTM databases

iPTMnetiQ9WUA2.
PhosphoSiteiQ9WUA2.
SwissPalmiQ9WUA2.

Proteomic databases

EPDiQ9WUA2.
MaxQBiQ9WUA2.
PaxDbiQ9WUA2.
PRIDEiQ9WUA2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000068333; ENSMUSP00000069508; ENSMUSG00000026245.
ENSMUST00000170217; ENSMUSP00000129828; ENSMUSG00000026245.
GeneIDi23874.
KEGGimmu:23874.
UCSCiuc007bqf.2. mouse.

Organism-specific databases

CTDi10056.
MGIiMGI:1346035. Farsb.

Phylogenomic databases

eggNOGiKOG2472. Eukaryota.
COG0072. LUCA.
GeneTreeiENSGT00530000063489.
HOGENOMiHOG000105095.
HOVERGENiHBG009523.
InParanoidiQ9WUA2.
KOiK01890.
OMAiERPYVTG.
OrthoDBiEOG7R2BJ6.
TreeFamiTF105681.

Miscellaneous databases

NextBioi303591.
PROiQ9WUA2.
SOURCEiSearch...

Gene expression databases

BgeeiQ9WUA2.
CleanExiMM_FARSB.
ExpressionAtlasiQ9WUA2. baseline and differential.
GenevisibleiQ9WUA2. MM.

Family and domain databases

Gene3Di3.30.56.20. 1 hit.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR004531. Phe-tRNA-synth_IIc_bsu_arc.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 2 hits.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR00471. pheT_arch. 1 hit.
PROSITEiPS51483. B5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the cDNA encoding phenylalanyl tRNA synthetase regulatory alpha-subunit-like protein whose expression is down-regulated during differentiation."
    Zhou X.-B., Richon V.M., Ngo L., Rifkind R.A., Marks P.A.
    Gene 233:13-19(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Corpora quadrigemina.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiSYFB_MOUSE
AccessioniPrimary (citable) accession number: Q9WUA2
Secondary accession number(s): Q9CWZ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.