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Protein

Proline dehydrogenase 1, mitochondrial

Gene

Prodh

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts proline to delta-1-pyrroline-5-carboxylate.

Catalytic activityi

L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol.

Cofactori

Pathwayi: L-proline degradation into L-glutamate

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate from L-proline.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable proline dehydrogenase 2 (Prodh2), Proline dehydrogenase 1, mitochondrial (Prodh)
  2. Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial (Aldh4a1)
This subpathway is part of the pathway L-proline degradation into L-glutamate, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate from L-proline, the pathway L-proline degradation into L-glutamate and in Amino-acid degradation.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Proline metabolism

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

ReactomeiR-MMU-70688. Proline catabolism.
UniPathwayiUPA00261; UER00373.

Names & Taxonomyi

Protein namesi
Recommended name:
Proline dehydrogenase 1, mitochondrial (EC:1.5.5.2)
Alternative name(s):
Proline oxidase
Gene namesi
Name:Prodh
Synonyms:Pro1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:97770. Prodh.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial inner membrane Source: MGI
  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Involvement in diseasei

Pro/re mice that have a premature termination on Prodh are sluggish in their movement.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 599Proline dehydrogenase 1, mitochondrialPRO_0000025801
Transit peptidei1 – ?MitochondrionSequence analysis

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei356 – 3561N6-acetyllysineCombined sources
Modified residuei367 – 3671N6-acetyllysineCombined sources
Modified residuei485 – 4851N6-acetyllysineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9WU79.
MaxQBiQ9WU79.
PaxDbiQ9WU79.
PeptideAtlasiQ9WU79.
PRIDEiQ9WU79.

PTM databases

iPTMnetiQ9WU79.
PhosphoSiteiQ9WU79.
SwissPalmiQ9WU79.

Expressioni

Tissue specificityi

Expressed in liver, kidney, heart and to a lesser extent in brain, lung and muscle.

Gene expression databases

BgeeiENSMUSG00000003526.
CleanExiMM_PRODH.
ExpressionAtlasiQ9WU79. baseline and differential.
GenevisibleiQ9WU79. MM.

Interactioni

Protein-protein interaction databases

IntActiQ9WU79. 3 interactions.
MINTiMINT-4130517.
STRINGi10090.ENSMUSP00000003620.

Structurei

3D structure databases

ProteinModelPortaliQ9WU79.
SMRiQ9WU79. Positions 349-580.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the proline oxidase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0186. Eukaryota.
COG0506. LUCA.
GeneTreeiENSGT00390000006265.
HOGENOMiHOG000233406.
HOVERGENiHBG008262.
InParanoidiQ9WU79.
KOiK00318.
OMAiRKEMESC.
OrthoDBiEOG091G05G3.
PhylomeDBiQ9WU79.
TreeFamiTF313544.

Family and domain databases

Gene3Di3.20.20.220. 3 hits.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR002872. Proline_DH_dom.
IPR015659. Proline_oxidase.
[Graphical view]
PANTHERiPTHR13914. PTHR13914. 2 hits.
PfamiPF01619. Pro_dh. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 3 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9WU79-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALKRVFLLR SVAPRVAALS TKPQAQEQPP ASPEALRGCG AAKAVRPPVP
60 70 80 90 100
AVDFTNTQEA YRSRRSWELV RNLLVLRLCA SPVLLAHHEQ LFQVARKLLG
110 120 130 140 150
QRMFERLMKM TFYGHFVAGE DQESIRPLIR HNKAFGVGFI LDYGVEEDLS
160 170 180 190 200
PEEAERKEME SCTSEAERDG SGANKREKQY QVHPAFGDRR DGVISARTYF
210 220 230 240 250
YANEAKCDNY MENLLQCIKA SGGASDGGFS AIKLTALGRP QFLLQFSDVL
260 270 280 290 300
TRWRRFFHQM AAEQGQAGRA AVDTKLEVAV LQDSIAKMGI ASRAEIEGWF
310 320 330 340 350
TPETLGVSGT VDLLDWNSLI DSRTRLSRHL VVPNVQTGQL EPLLSRFTEE
360 370 380 390 400
EEQQMKRMLQ RMDVLAKKAK EAGVRLMIDA EQSYFQPAIS RLTLEMQRRF
410 420 430 440 450
NVDKPFIFNT FQCYLKDAYD NVTLDMELAR REGWCFGAKL VRGAYMAQER
460 470 480 490 500
VRAAEIGYED PINPTYEATN AMYHRCLNYV LEELKHSTKA EVMVASHNED
510 520 530 540 550
TVHFTLCRMK EIGLHPADGQ VCFGQLLGMC DQISFPLGQA GFPVYKYVPY
560 570 580 590
GPVMEVLPYL SRRALENSSI MKGAQRERQL LWQELRRRLR TGSLFHHPA
Length:599
Mass (Da):68,036
Last modified:April 5, 2011 - v2
Checksum:i528AA436920CE5DD
GO

Sequence cautioni

The sequence AAD24776 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAI25328 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence EDK97501 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti7 – 82FL → HE in AAF21467 (Ref. 5) Curated
Sequence conflicti303 – 3031E → V in BAE25683 (PubMed:16141072).Curated
Sequence conflicti379 – 3791D → G in BAE25683 (PubMed:16141072).Curated
Sequence conflicti436 – 4361F → S in AAD24776 (PubMed:10192398).Curated
Sequence conflicti443 – 4431G → R in AAD24776 (PubMed:10192398).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK144068 mRNA. Translation: BAE25683.1.
AC087064 Genomic DNA. No translation available.
CH466521 Genomic DNA. Translation: EDK97501.1. Sequence problems.
AF120279 mRNA. Translation: AAD24776.1. Different initiation.
U80020 mRNA. Translation: AAF21467.1.
BC125327 mRNA. Translation: AAI25328.1. Different initiation.
CCDSiCCDS37278.1.
RefSeqiNP_035302.2. NM_011172.2.
UniGeneiMm.28456.

Genome annotation databases

EnsembliENSMUST00000003620; ENSMUSP00000003620; ENSMUSG00000003526.
GeneIDi19125.
KEGGimmu:19125.
UCSCiuc007ymu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK144068 mRNA. Translation: BAE25683.1.
AC087064 Genomic DNA. No translation available.
CH466521 Genomic DNA. Translation: EDK97501.1. Sequence problems.
AF120279 mRNA. Translation: AAD24776.1. Different initiation.
U80020 mRNA. Translation: AAF21467.1.
BC125327 mRNA. Translation: AAI25328.1. Different initiation.
CCDSiCCDS37278.1.
RefSeqiNP_035302.2. NM_011172.2.
UniGeneiMm.28456.

3D structure databases

ProteinModelPortaliQ9WU79.
SMRiQ9WU79. Positions 349-580.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9WU79. 3 interactions.
MINTiMINT-4130517.
STRINGi10090.ENSMUSP00000003620.

PTM databases

iPTMnetiQ9WU79.
PhosphoSiteiQ9WU79.
SwissPalmiQ9WU79.

Proteomic databases

EPDiQ9WU79.
MaxQBiQ9WU79.
PaxDbiQ9WU79.
PeptideAtlasiQ9WU79.
PRIDEiQ9WU79.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003620; ENSMUSP00000003620; ENSMUSG00000003526.
GeneIDi19125.
KEGGimmu:19125.
UCSCiuc007ymu.1. mouse.

Organism-specific databases

CTDi5625.
MGIiMGI:97770. Prodh.

Phylogenomic databases

eggNOGiKOG0186. Eukaryota.
COG0506. LUCA.
GeneTreeiENSGT00390000006265.
HOGENOMiHOG000233406.
HOVERGENiHBG008262.
InParanoidiQ9WU79.
KOiK00318.
OMAiRKEMESC.
OrthoDBiEOG091G05G3.
PhylomeDBiQ9WU79.
TreeFamiTF313544.

Enzyme and pathway databases

UniPathwayiUPA00261; UER00373.
ReactomeiR-MMU-70688. Proline catabolism.

Miscellaneous databases

PROiQ9WU79.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000003526.
CleanExiMM_PRODH.
ExpressionAtlasiQ9WU79. baseline and differential.
GenevisibleiQ9WU79. MM.

Family and domain databases

Gene3Di3.20.20.220. 3 hits.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR002872. Proline_DH_dom.
IPR015659. Proline_oxidase.
[Graphical view]
PANTHERiPTHR13914. PTHR13914. 2 hits.
PfamiPF01619. Pro_dh. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiPROD_MOUSE
AccessioniPrimary (citable) accession number: Q9WU79
Secondary accession number(s): A0JLW6, Q3UNR4, Q9QX61
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: April 5, 2011
Last modified: September 7, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.