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Protein

Programmed cell death 6-interacting protein

Gene

Pdcd6ip

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. May play a role in the regulation of both apoptosis and cell proliferation. Regulates exosome biogenesis in concert with SDC1/4 and SDCBP (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis, Cell cycle, Cell division, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Programmed cell death 6-interacting protein
Alternative name(s):
ALG-2-interacting protein 1
ALG-2-interacting protein X
E2F1-inducible protein
Eig2
Gene namesi
Name:Pdcd6ip
Synonyms:Aip1, Alix
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1333753. Pdcd6ip.

Subcellular locationi

  • Cytoplasmcytosol By similarity
  • Melanosome By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
  • Secretedexosome By similarity

  • Note: Colocalized with CEP55 in the midbody during cytokinesis. Colocalized with CEP55 at centrosomes of non-dividing cells (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002188922 – 869Programmed cell death 6-interacting proteinAdd BLAST868

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei215N6-acetyllysineBy similarity1
Modified residuei479PhosphothreonineBy similarity1
Modified residuei481PhosphoserineBy similarity1
Modified residuei730PhosphoserineBy similarity1
Modified residuei741PhosphothreonineBy similarity1
Modified residuei745Omega-N-methylarginineBy similarity1

Post-translational modificationi

May be phosphorylated on tyrosine residues by activated PDGFRB.By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ9WU78.
PaxDbiQ9WU78.
PeptideAtlasiQ9WU78.
PRIDEiQ9WU78.

2D gel databases

REPRODUCTION-2DPAGEQ9WU78.
UCD-2DPAGEQ9WU78.

PTM databases

iPTMnetiQ9WU78.
PhosphoSitePlusiQ9WU78.
SwissPalmiQ9WU78.

Expressioni

Tissue specificityi

Ubiquitously expressed.

Gene expression databases

BgeeiENSMUSG00000032504.
CleanExiMM_PDCD6IP.
GenevisibleiQ9WU78. MM.

Interactioni

Subunit structurei

Interacts with SH3KBP1. Interacts with PDCD6; the interaction is calcium-dependent. Interacts with TSG101 (By similarity). Interacts with SGSM3. Self-associates. Interacts with CHMP4A; the interaction is direct. Interacts with CHMP4B; the interaction is direct. Interacts with CHMP4C; the interaction is direct. Interacts with CEP55; the interaction is direct; CEP55 binds PDCD6IP in a 2:1 stoichiometry. Interacts with SH3GL1 and SH3GL2. Interacts with PDGFRB (By similarity). Interacts with SDCBP (PubMed:22660413). Forms a complex with SDCBP and SDC2 (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Plcg2Q8CIH57EBI-641897,EBI-617954
SdcbpO089923EBI-641897,EBI-538265

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202072. 5 interactors.
DIPiDIP-41418N.
IntActiQ9WU78. 12 interactors.
MINTiMINT-238635.
STRINGi10090.ENSMUSP00000107492.

Structurei

3D structure databases

ProteinModelPortaliQ9WU78.
SMRiQ9WU78.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 392BRO1PROSITE-ProRule annotationAdd BLAST390

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni176 – 503Interaction with CHMP4A, CHMP4B and CHMP4CBy similarityAdd BLAST328
Regioni383 – 869Interaction with SDCBP1 PublicationAdd BLAST487
Regioni503 – 869Self-associationBy similarityAdd BLAST367
Regioni717 – 720Interaction with TSG101By similarity4
Regioni798 – 807Interaction with CEP55By similarity10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi717 – 861Pro-richAdd BLAST145

Sequence similaritiesi

Contains 1 BRO1 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2220. Eukaryota.
ENOG410XQX6. LUCA.
GeneTreeiENSGT00780000121909.
HOVERGENiHBG053533.
InParanoidiQ9WU78.
KOiK12200.
OMAiQCIMQAN.
OrthoDBiEOG091G04NC.
TreeFamiTF323502.

Family and domain databases

Gene3Di1.25.40.280. 1 hit.
InterProiIPR025304. ALIX_V_dom.
IPR004328. BRO1_dom.
[Graphical view]
PfamiPF13949. ALIX_LYPXL_bnd. 1 hit.
PF03097. BRO1. 1 hit.
[Graphical view]
SMARTiSM01041. BRO1. 1 hit.
[Graphical view]
PROSITEiPS51180. BRO1. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9WU78-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASFIWVQLK KTSEVDLAKP LVKFIQQTYP SGGEEQAQYC RAAEELSKLR
60 70 80 90 100
RSALGRPLDK HEGALETLLR YYDQICSIEP KFPFSENQIC LTFTWKDAFD
110 120 130 140 150
KGSLFGGSVK LALASLGYEK SCVLFNCAAL ASQIAAEQNL DNDEGLKTAA
160 170 180 190 200
KQYQFASGAF LHIKDTVLSA LSREPTVDIS PDTVGTLSLI MLAQAQEVFF
210 220 230 240 250
LKATRDKMKD AIIAKLANQA ADYFGDAFKQ CQYKDTLPKE VFPTLAAKQC
260 270 280 290 300
IMQANAEYHQ SILAKQQKKF GEEIARLQHA AELIKNVASR YDEYVNVKDF
310 320 330 340 350
SDKINRALTA AKKDNDFIYH DRVPDLKDLD PIGKATLVKP TPVNVPVSQK
360 370 380 390 400
FTDLFEKMVP VSVQQSLAVF SQRKADLVNR SIAQMREATT LANGVLASLN
410 420 430 440 450
LPAAIEDVSG DTVPQSILTK STSVVEQGGI QTVDQLIKEL PELLQRNREI
460 470 480 490 500
LEESLRLLDE EEATDNDLRA KFKDRWQRTP SNDLYKPLRA EGAKFRAVLD
510 520 530 540 550
KAVQADGQVK ERYQSHRDTI ALLCKPEPEL NAAIPSANPA KTMQGSEVVS
560 570 580 590 600
VLKSLLSNLD EIKKERESLE NDLKSVNFDM TSKFLTALAQ DGVINEEALS
610 620 630 640 650
VTELDRIYGG LTSKVQESLK KQEGLLKNIQ VSHQEFSKMK QSNNEANLRE
660 670 680 690 700
EVLKNLATAY DNFVELVANL KEGTKFYNEL TEILVRFQNK CSDIVFARKT
710 720 730 740 750
ERDELLKDLQ QSIAREPSAP SIPPPAYQSS PAAGHAAAPP TPAPRTMPPA
760 770 780 790 800
KPQPPARPPP PVLPANRVPP ASAAAAPAGV GTASAAPPQT PGSAPPPQAQ
810 820 830 840 850
GPPYPTYPGY PGYCQMPMPM GYNPYAYGQY NMPYPPVYHQ SPGQAPYPGP
860
QQPTYPFPQP PQQSYYPQQ
Length:869
Mass (Da):96,024
Last modified:July 27, 2011 - v3
Checksum:iBFDC87ACA183C5D4
GO
Isoform 2 (identifier: Q9WU78-2) [UniParc]FASTAAdd to basket
Also known as: Alix-SF, Short

The sequence of this isoform differs from the canonical sequence as follows:
     159-805: Missing.

Note: Does not interact with ALG-2.
Show »
Length:222
Mass (Da):24,924
Checksum:i35BEBFF783AF1519
GO
Isoform 3 (identifier: Q9WU78-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     239-239: K → KYFYFQ

Show »
Length:874
Mass (Da):96,773
Checksum:i4B3AA18F84E39723
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti329 – 333LDPIG → SGSYR in AAD26813 (PubMed:9880530).Curated5
Sequence conflicti530L → V in CAA06329 (PubMed:10200558).Curated1
Sequence conflicti547 – 548EV → DL in AAD26813 (PubMed:9880530).Curated2
Sequence conflicti595N → T in AAH26823 (PubMed:15489334).Curated1
Sequence conflicti625L → V in AAD26813 (PubMed:9880530).Curated1
Sequence conflicti640 – 641KQ → NE in AAD26813 (PubMed:9880530).Curated2
Sequence conflicti821G → R in AAD26813 (PubMed:9880530).Curated1
Sequence conflicti853P → L in CAA06330 (PubMed:10200558).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007502159 – 805Missing in isoform 2. 1 PublicationAdd BLAST647
Alternative sequenceiVSP_007501239K → KYFYFQ in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005073 mRNA. Translation: CAA06329.1.
AJ005074 mRNA. Translation: CAA06330.1.
AF119955 mRNA. Translation: AAD26813.1.
AK031256 mRNA. Translation: BAC27323.1.
AK167574 mRNA. Translation: BAE39637.1.
AK169704 mRNA. Translation: BAE41316.1.
CT025751, AC162177, AC167246 Genomic DNA. Translation: CAX15748.1.
CH466621 Genomic DNA. Translation: EDL08950.1.
BC002261 mRNA. Translation: AAH02261.1.
BC026823 mRNA. Translation: AAH26823.1.
AF176514 mRNA. Translation: AAD53115.1.
CCDSiCCDS23588.1. [Q9WU78-1]
CCDS52947.1. [Q9WU78-3]
RefSeqiNP_001158149.1. NM_001164677.1. [Q9WU78-3]
NP_001158150.1. NM_001164678.1.
NP_035182.2. NM_011052.2. [Q9WU78-1]
UniGeneiMm.29816.

Genome annotation databases

EnsembliENSMUST00000035086; ENSMUSP00000035086; ENSMUSG00000032504. [Q9WU78-1]
ENSMUST00000111861; ENSMUSP00000107492; ENSMUSG00000032504. [Q9WU78-3]
GeneIDi18571.
KEGGimmu:18571.
UCSCiuc009rwn.2. mouse. [Q9WU78-1]
uc009rwo.2. mouse. [Q9WU78-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005073 mRNA. Translation: CAA06329.1.
AJ005074 mRNA. Translation: CAA06330.1.
AF119955 mRNA. Translation: AAD26813.1.
AK031256 mRNA. Translation: BAC27323.1.
AK167574 mRNA. Translation: BAE39637.1.
AK169704 mRNA. Translation: BAE41316.1.
CT025751, AC162177, AC167246 Genomic DNA. Translation: CAX15748.1.
CH466621 Genomic DNA. Translation: EDL08950.1.
BC002261 mRNA. Translation: AAH02261.1.
BC026823 mRNA. Translation: AAH26823.1.
AF176514 mRNA. Translation: AAD53115.1.
CCDSiCCDS23588.1. [Q9WU78-1]
CCDS52947.1. [Q9WU78-3]
RefSeqiNP_001158149.1. NM_001164677.1. [Q9WU78-3]
NP_001158150.1. NM_001164678.1.
NP_035182.2. NM_011052.2. [Q9WU78-1]
UniGeneiMm.29816.

3D structure databases

ProteinModelPortaliQ9WU78.
SMRiQ9WU78.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202072. 5 interactors.
DIPiDIP-41418N.
IntActiQ9WU78. 12 interactors.
MINTiMINT-238635.
STRINGi10090.ENSMUSP00000107492.

PTM databases

iPTMnetiQ9WU78.
PhosphoSitePlusiQ9WU78.
SwissPalmiQ9WU78.

2D gel databases

REPRODUCTION-2DPAGEQ9WU78.
UCD-2DPAGEQ9WU78.

Proteomic databases

EPDiQ9WU78.
PaxDbiQ9WU78.
PeptideAtlasiQ9WU78.
PRIDEiQ9WU78.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035086; ENSMUSP00000035086; ENSMUSG00000032504. [Q9WU78-1]
ENSMUST00000111861; ENSMUSP00000107492; ENSMUSG00000032504. [Q9WU78-3]
GeneIDi18571.
KEGGimmu:18571.
UCSCiuc009rwn.2. mouse. [Q9WU78-1]
uc009rwo.2. mouse. [Q9WU78-3]

Organism-specific databases

CTDi10015.
MGIiMGI:1333753. Pdcd6ip.

Phylogenomic databases

eggNOGiKOG2220. Eukaryota.
ENOG410XQX6. LUCA.
GeneTreeiENSGT00780000121909.
HOVERGENiHBG053533.
InParanoidiQ9WU78.
KOiK12200.
OMAiQCIMQAN.
OrthoDBiEOG091G04NC.
TreeFamiTF323502.

Miscellaneous databases

ChiTaRSiPdcd6ip. mouse.
PROiQ9WU78.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032504.
CleanExiMM_PDCD6IP.
GenevisibleiQ9WU78. MM.

Family and domain databases

Gene3Di1.25.40.280. 1 hit.
InterProiIPR025304. ALIX_V_dom.
IPR004328. BRO1_dom.
[Graphical view]
PfamiPF13949. ALIX_LYPXL_bnd. 1 hit.
PF03097. BRO1. 1 hit.
[Graphical view]
SMARTiSM01041. BRO1. 1 hit.
[Graphical view]
PROSITEiPS51180. BRO1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPDC6I_MOUSE
AccessioniPrimary (citable) accession number: Q9WU78
Secondary accession number(s): O88695
, O89014, Q3TED2, Q8BSL8, Q8R0H5, Q99LR3, Q9QZN8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 132 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.