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Q9WU78

- PDC6I_MOUSE

UniProt

Q9WU78 - PDC6I_MOUSE

Protein

Programmed cell death 6-interacting protein

Gene

Pdcd6ip

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. May play a role in the regulation of both apoptosis and cell proliferation.

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein dimerization activity Source: UniProtKB

    GO - Biological processi

    1. apoptotic process Source: MGI
    2. cell cycle Source: UniProtKB-KW
    3. cell division Source: UniProtKB-KW
    4. protein transport Source: UniProtKB-KW

    Keywords - Biological processi

    Apoptosis, Cell cycle, Cell division, Protein transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Programmed cell death 6-interacting protein
    Alternative name(s):
    ALG-2-interacting protein 1
    ALG-2-interacting protein X
    E2F1-inducible protein
    Eig2
    Gene namesi
    Name:Pdcd6ip
    Synonyms:Aip1, Alix
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:1333753. Pdcd6ip.

    Subcellular locationi

    Cytoplasmcytosol By similarity. Melanosome By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
    Note: Colocalized with CEP55 in the midbody during cytokinesis. Colocalized with CEP55 at centrosomes of non-dividing cells By similarity.By similarity

    GO - Cellular componenti

    1. cytosol Source: MGI
    2. melanosome Source: UniProtKB-SubCell
    3. microtubule organizing center Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 869868Programmed cell death 6-interacting proteinPRO_0000218892Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei215 – 2151N6-acetyllysineBy similarity
    Modified residuei730 – 7301PhosphoserineBy similarity
    Modified residuei741 – 7411PhosphothreonineBy similarity

    Post-translational modificationi

    May be phosphorylated on tyrosine residues by activated PDGFRB.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ9WU78.
    PaxDbiQ9WU78.
    PRIDEiQ9WU78.

    2D gel databases

    REPRODUCTION-2DPAGEQ9WU78.
    UCD-2DPAGEQ9WU78.

    PTM databases

    PhosphoSiteiQ9WU78.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed.

    Gene expression databases

    ArrayExpressiQ9WU78.
    BgeeiQ9WU78.
    CleanExiMM_PDCD6IP.
    GenevestigatoriQ9WU78.

    Interactioni

    Subunit structurei

    Interacts with SH3KBP1. Interacts with PDCD6; the interaction is calcium-dependent. Interacts with TSG101 By similarity. Interacts with SGSM3. Self-associates. Interacts with CHMP4A; the interaction is direct. Interacts with CHMP4B; the interaction is direct. Interacts with CHMP4C; the interaction is direct. Interacts with CEP55; the interaction is direct; CEP55 binds PDCD6IP in a 2:1 stoichiometry. Interacts with SH3GL1 and SH3GL2. Interacts with PDGFRB By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Plcg2Q8CIH57EBI-641897,EBI-617954

    Protein-protein interaction databases

    BioGridi202072. 5 interactions.
    DIPiDIP-41418N.
    IntActiQ9WU78. 10 interactions.
    MINTiMINT-238635.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9WU78.
    SMRiQ9WU78. Positions 2-698.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 392390BRO1PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni176 – 503328Interaction with CHMP4A, CHMP4B and CHMP4CBy similarityAdd
    BLAST
    Regioni503 – 869367Self-associationBy similarityAdd
    BLAST
    Regioni717 – 7204Interaction with TSG101By similarity
    Regioni798 – 80710Interaction with CEP55By similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi717 – 861145Pro-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 BRO1 domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG325528.
    GeneTreeiENSGT00750000117459.
    HOVERGENiHBG053533.
    KOiK12200.
    OMAiQVKECYQ.
    OrthoDBiEOG7V49XV.
    TreeFamiTF323502.

    Family and domain databases

    Gene3Di1.25.40.280. 1 hit.
    InterProiIPR025304. ALIX_V_dom.
    IPR004328. BRO1_dom.
    [Graphical view]
    PfamiPF13949. ALIX_LYPXL_bnd. 1 hit.
    PF03097. BRO1. 1 hit.
    [Graphical view]
    SMARTiSM01041. BRO1. 1 hit.
    [Graphical view]
    PROSITEiPS51180. BRO1. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9WU78-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MASFIWVQLK KTSEVDLAKP LVKFIQQTYP SGGEEQAQYC RAAEELSKLR    50
    RSALGRPLDK HEGALETLLR YYDQICSIEP KFPFSENQIC LTFTWKDAFD 100
    KGSLFGGSVK LALASLGYEK SCVLFNCAAL ASQIAAEQNL DNDEGLKTAA 150
    KQYQFASGAF LHIKDTVLSA LSREPTVDIS PDTVGTLSLI MLAQAQEVFF 200
    LKATRDKMKD AIIAKLANQA ADYFGDAFKQ CQYKDTLPKE VFPTLAAKQC 250
    IMQANAEYHQ SILAKQQKKF GEEIARLQHA AELIKNVASR YDEYVNVKDF 300
    SDKINRALTA AKKDNDFIYH DRVPDLKDLD PIGKATLVKP TPVNVPVSQK 350
    FTDLFEKMVP VSVQQSLAVF SQRKADLVNR SIAQMREATT LANGVLASLN 400
    LPAAIEDVSG DTVPQSILTK STSVVEQGGI QTVDQLIKEL PELLQRNREI 450
    LEESLRLLDE EEATDNDLRA KFKDRWQRTP SNDLYKPLRA EGAKFRAVLD 500
    KAVQADGQVK ERYQSHRDTI ALLCKPEPEL NAAIPSANPA KTMQGSEVVS 550
    VLKSLLSNLD EIKKERESLE NDLKSVNFDM TSKFLTALAQ DGVINEEALS 600
    VTELDRIYGG LTSKVQESLK KQEGLLKNIQ VSHQEFSKMK QSNNEANLRE 650
    EVLKNLATAY DNFVELVANL KEGTKFYNEL TEILVRFQNK CSDIVFARKT 700
    ERDELLKDLQ QSIAREPSAP SIPPPAYQSS PAAGHAAAPP TPAPRTMPPA 750
    KPQPPARPPP PVLPANRVPP ASAAAAPAGV GTASAAPPQT PGSAPPPQAQ 800
    GPPYPTYPGY PGYCQMPMPM GYNPYAYGQY NMPYPPVYHQ SPGQAPYPGP 850
    QQPTYPFPQP PQQSYYPQQ 869
    Length:869
    Mass (Da):96,024
    Last modified:July 27, 2011 - v3
    Checksum:iBFDC87ACA183C5D4
    GO
    Isoform 2 (identifier: Q9WU78-2) [UniParc]FASTAAdd to Basket

    Also known as: Alix-SF, Short

    The sequence of this isoform differs from the canonical sequence as follows:
         159-805: Missing.

    Note: Does not interact with ALG-2.

    Show »
    Length:222
    Mass (Da):24,924
    Checksum:i35BEBFF783AF1519
    GO
    Isoform 3 (identifier: Q9WU78-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         239-239: K → KYFYFQ

    Show »
    Length:874
    Mass (Da):96,773
    Checksum:i4B3AA18F84E39723
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti329 – 3335LDPIG → SGSYR in AAD26813. (PubMed:9880530)Curated
    Sequence conflicti530 – 5301L → V in CAA06329. (PubMed:10200558)Curated
    Sequence conflicti547 – 5482EV → DL in AAD26813. (PubMed:9880530)Curated
    Sequence conflicti595 – 5951N → T in AAH26823. (PubMed:15489334)Curated
    Sequence conflicti625 – 6251L → V in AAD26813. (PubMed:9880530)Curated
    Sequence conflicti640 – 6412KQ → NE in AAD26813. (PubMed:9880530)Curated
    Sequence conflicti821 – 8211G → R in AAD26813. (PubMed:9880530)Curated
    Sequence conflicti853 – 8531P → L in CAA06330. (PubMed:10200558)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei159 – 805647Missing in isoform 2. 1 PublicationVSP_007502Add
    BLAST
    Alternative sequencei239 – 2391K → KYFYFQ in isoform 3. 1 PublicationVSP_007501

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ005073 mRNA. Translation: CAA06329.1.
    AJ005074 mRNA. Translation: CAA06330.1.
    AF119955 mRNA. Translation: AAD26813.1.
    AK031256 mRNA. Translation: BAC27323.1.
    AK167574 mRNA. Translation: BAE39637.1.
    AK169704 mRNA. Translation: BAE41316.1.
    CT025751, AC162177, AC167246 Genomic DNA. Translation: CAX15748.1.
    CH466621 Genomic DNA. Translation: EDL08950.1.
    BC002261 mRNA. Translation: AAH02261.1.
    BC026823 mRNA. Translation: AAH26823.1.
    AF176514 mRNA. Translation: AAD53115.1.
    CCDSiCCDS23588.1. [Q9WU78-1]
    CCDS52947.1. [Q9WU78-3]
    RefSeqiNP_001158149.1. NM_001164677.1. [Q9WU78-3]
    NP_001158150.1. NM_001164678.1.
    NP_035182.2. NM_011052.2. [Q9WU78-1]
    UniGeneiMm.29816.

    Genome annotation databases

    EnsembliENSMUST00000035086; ENSMUSP00000035086; ENSMUSG00000032504. [Q9WU78-1]
    ENSMUST00000111861; ENSMUSP00000107492; ENSMUSG00000032504. [Q9WU78-3]
    GeneIDi18571.
    KEGGimmu:18571.
    UCSCiuc009rwn.2. mouse. [Q9WU78-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ005073 mRNA. Translation: CAA06329.1 .
    AJ005074 mRNA. Translation: CAA06330.1 .
    AF119955 mRNA. Translation: AAD26813.1 .
    AK031256 mRNA. Translation: BAC27323.1 .
    AK167574 mRNA. Translation: BAE39637.1 .
    AK169704 mRNA. Translation: BAE41316.1 .
    CT025751 , AC162177 , AC167246 Genomic DNA. Translation: CAX15748.1 .
    CH466621 Genomic DNA. Translation: EDL08950.1 .
    BC002261 mRNA. Translation: AAH02261.1 .
    BC026823 mRNA. Translation: AAH26823.1 .
    AF176514 mRNA. Translation: AAD53115.1 .
    CCDSi CCDS23588.1. [Q9WU78-1 ]
    CCDS52947.1. [Q9WU78-3 ]
    RefSeqi NP_001158149.1. NM_001164677.1. [Q9WU78-3 ]
    NP_001158150.1. NM_001164678.1.
    NP_035182.2. NM_011052.2. [Q9WU78-1 ]
    UniGenei Mm.29816.

    3D structure databases

    ProteinModelPortali Q9WU78.
    SMRi Q9WU78. Positions 2-698.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202072. 5 interactions.
    DIPi DIP-41418N.
    IntActi Q9WU78. 10 interactions.
    MINTi MINT-238635.

    PTM databases

    PhosphoSitei Q9WU78.

    2D gel databases

    REPRODUCTION-2DPAGE Q9WU78.
    UCD-2DPAGE Q9WU78.

    Proteomic databases

    MaxQBi Q9WU78.
    PaxDbi Q9WU78.
    PRIDEi Q9WU78.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000035086 ; ENSMUSP00000035086 ; ENSMUSG00000032504 . [Q9WU78-1 ]
    ENSMUST00000111861 ; ENSMUSP00000107492 ; ENSMUSG00000032504 . [Q9WU78-3 ]
    GeneIDi 18571.
    KEGGi mmu:18571.
    UCSCi uc009rwn.2. mouse. [Q9WU78-1 ]

    Organism-specific databases

    CTDi 10015.
    MGIi MGI:1333753. Pdcd6ip.

    Phylogenomic databases

    eggNOGi NOG325528.
    GeneTreei ENSGT00750000117459.
    HOVERGENi HBG053533.
    KOi K12200.
    OMAi QVKECYQ.
    OrthoDBi EOG7V49XV.
    TreeFami TF323502.

    Miscellaneous databases

    ChiTaRSi PDCD6IP. mouse.
    NextBioi 294408.
    PROi Q9WU78.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9WU78.
    Bgeei Q9WU78.
    CleanExi MM_PDCD6IP.
    Genevestigatori Q9WU78.

    Family and domain databases

    Gene3Di 1.25.40.280. 1 hit.
    InterProi IPR025304. ALIX_V_dom.
    IPR004328. BRO1_dom.
    [Graphical view ]
    Pfami PF13949. ALIX_LYPXL_bnd. 1 hit.
    PF03097. BRO1. 1 hit.
    [Graphical view ]
    SMARTi SM01041. BRO1. 1 hit.
    [Graphical view ]
    PROSITEi PS51180. BRO1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Alix, a novel mouse protein undergoing calcium-dependent interaction with the apoptotsis-linked-gene 2 (ALG-2) protein."
      Missotten M., Nichols A., Rieger K., Sadoul R.
      Cell Death Differ. 6:124-129(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), INTERACTION WITH PDCD6.
      Tissue: Brain.
    2. "Cloning of AIP1, a novel protein that associates with the apoptosis-linked gene ALG-2 in a Ca2+-dependent reaction."
      Vito P., Pellegrini L., Guiet C., D'Adamio L.
      J. Biol. Chem. 274:1533-1540(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
      Strain: C57BL/6J and NOD.
      Tissue: Forelimb, Placenta and Thymus.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Mammary gland.
    7. "Rapid analysis of gene expression (RAGE) facilitates universal expression profiling."
      Wang A.J., Pierce A., Judson-Kremer K., Gaddis S., Aldaz C.M., Johnson D.G., MacLeod M.C.
      Nucleic Acids Res. 27:4609-4618(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 671-869.
      Strain: C57BL/6J.
      Tissue: Forelimb.

    Entry informationi

    Entry nameiPDC6I_MOUSE
    AccessioniPrimary (citable) accession number: Q9WU78
    Secondary accession number(s): O88695
    , O89014, Q3TED2, Q8BSL8, Q8R0H5, Q99LR3, Q9QZN8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 23, 2003
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 111 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3