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Q9WU78

- PDC6I_MOUSE

UniProt

Q9WU78 - PDC6I_MOUSE

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Protein
Programmed cell death 6-interacting protein
Gene
Pdcd6ip, Aip1, Alix
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. May play a role in the regulation of both apoptosis and cell proliferation.

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. protein dimerization activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. apoptotic process Source: MGI
  2. cell cycle Source: UniProtKB-KW
  3. cell division Source: UniProtKB-KW
  4. protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Apoptosis, Cell cycle, Cell division, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Programmed cell death 6-interacting protein
Alternative name(s):
ALG-2-interacting protein 1
ALG-2-interacting protein X
E2F1-inducible protein
Eig2
Gene namesi
Name:Pdcd6ip
Synonyms:Aip1, Alix
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:1333753. Pdcd6ip.

Subcellular locationi

Cytoplasmcytosol By similarity. Melanosome By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
Note: Colocalized with CEP55 in the midbody during cytokinesis. Colocalized with CEP55 at centrosomes of non-dividing cells By similarity.

GO - Cellular componenti

  1. cytosol Source: MGI
  2. melanosome Source: UniProtKB-SubCell
  3. microtubule organizing center Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 869868Programmed cell death 6-interacting protein
PRO_0000218892Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Modified residuei215 – 2151N6-acetyllysine By similarity
Modified residuei730 – 7301Phosphoserine By similarity
Modified residuei741 – 7411Phosphothreonine By similarity

Post-translational modificationi

May be phosphorylated on tyrosine residues by activated PDGFRB By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9WU78.
PaxDbiQ9WU78.
PRIDEiQ9WU78.

2D gel databases

REPRODUCTION-2DPAGEQ9WU78.
UCD-2DPAGEQ9WU78.

PTM databases

PhosphoSiteiQ9WU78.

Expressioni

Tissue specificityi

Ubiquitously expressed.

Gene expression databases

ArrayExpressiQ9WU78.
BgeeiQ9WU78.
CleanExiMM_PDCD6IP.
GenevestigatoriQ9WU78.

Interactioni

Subunit structurei

Interacts with SH3KBP1. Interacts with PDCD6; the interaction is calcium-dependent. Interacts with TSG101 By similarity. Interacts with SGSM3. Self-associates. Interacts with CHMP4A; the interaction is direct. Interacts with CHMP4B; the interaction is direct. Interacts with CHMP4C; the interaction is direct. Interacts with CEP55; the interaction is direct; CEP55 binds PDCD6IP in a 2:1 stoichiometry. Interacts with SH3GL1 and SH3GL2. Interacts with PDGFRB By similarity.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Plcg2Q8CIH57EBI-641897,EBI-617954

Protein-protein interaction databases

BioGridi202072. 5 interactions.
DIPiDIP-41418N.
IntActiQ9WU78. 10 interactions.
MINTiMINT-238635.

Structurei

3D structure databases

ProteinModelPortaliQ9WU78.
SMRiQ9WU78. Positions 2-698.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 392390BRO1
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni176 – 503328Interaction with CHMP4A, CHMP4B and CHMP4C By similarity
Add
BLAST
Regioni503 – 869367Self-association By similarity
Add
BLAST
Regioni717 – 7204Interaction with TSG101 By similarity
Regioni798 – 80710Interaction with CEP55 By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi717 – 861145Pro-rich
Add
BLAST

Sequence similaritiesi

Contains 1 BRO1 domain.

Phylogenomic databases

eggNOGiNOG325528.
GeneTreeiENSGT00750000117459.
HOVERGENiHBG053533.
KOiK12200.
OMAiQVKECYQ.
OrthoDBiEOG7V49XV.
TreeFamiTF323502.

Family and domain databases

Gene3Di1.25.40.280. 1 hit.
InterProiIPR025304. ALIX_V_dom.
IPR004328. BRO1_dom.
[Graphical view]
PfamiPF13949. ALIX_LYPXL_bnd. 1 hit.
PF03097. BRO1. 1 hit.
[Graphical view]
SMARTiSM01041. BRO1. 1 hit.
[Graphical view]
PROSITEiPS51180. BRO1. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9WU78-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MASFIWVQLK KTSEVDLAKP LVKFIQQTYP SGGEEQAQYC RAAEELSKLR    50
RSALGRPLDK HEGALETLLR YYDQICSIEP KFPFSENQIC LTFTWKDAFD 100
KGSLFGGSVK LALASLGYEK SCVLFNCAAL ASQIAAEQNL DNDEGLKTAA 150
KQYQFASGAF LHIKDTVLSA LSREPTVDIS PDTVGTLSLI MLAQAQEVFF 200
LKATRDKMKD AIIAKLANQA ADYFGDAFKQ CQYKDTLPKE VFPTLAAKQC 250
IMQANAEYHQ SILAKQQKKF GEEIARLQHA AELIKNVASR YDEYVNVKDF 300
SDKINRALTA AKKDNDFIYH DRVPDLKDLD PIGKATLVKP TPVNVPVSQK 350
FTDLFEKMVP VSVQQSLAVF SQRKADLVNR SIAQMREATT LANGVLASLN 400
LPAAIEDVSG DTVPQSILTK STSVVEQGGI QTVDQLIKEL PELLQRNREI 450
LEESLRLLDE EEATDNDLRA KFKDRWQRTP SNDLYKPLRA EGAKFRAVLD 500
KAVQADGQVK ERYQSHRDTI ALLCKPEPEL NAAIPSANPA KTMQGSEVVS 550
VLKSLLSNLD EIKKERESLE NDLKSVNFDM TSKFLTALAQ DGVINEEALS 600
VTELDRIYGG LTSKVQESLK KQEGLLKNIQ VSHQEFSKMK QSNNEANLRE 650
EVLKNLATAY DNFVELVANL KEGTKFYNEL TEILVRFQNK CSDIVFARKT 700
ERDELLKDLQ QSIAREPSAP SIPPPAYQSS PAAGHAAAPP TPAPRTMPPA 750
KPQPPARPPP PVLPANRVPP ASAAAAPAGV GTASAAPPQT PGSAPPPQAQ 800
GPPYPTYPGY PGYCQMPMPM GYNPYAYGQY NMPYPPVYHQ SPGQAPYPGP 850
QQPTYPFPQP PQQSYYPQQ 869
Length:869
Mass (Da):96,024
Last modified:July 27, 2011 - v3
Checksum:iBFDC87ACA183C5D4
GO
Isoform 2 (identifier: Q9WU78-2) [UniParc]FASTAAdd to Basket

Also known as: Alix-SF, Short

The sequence of this isoform differs from the canonical sequence as follows:
     159-805: Missing.

Note: Does not interact with ALG-2.

Show »
Length:222
Mass (Da):24,924
Checksum:i35BEBFF783AF1519
GO
Isoform 3 (identifier: Q9WU78-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     239-239: K → KYFYFQ

Show »
Length:874
Mass (Da):96,773
Checksum:i4B3AA18F84E39723
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei159 – 805647Missing in isoform 2.
VSP_007502Add
BLAST
Alternative sequencei239 – 2391K → KYFYFQ in isoform 3.
VSP_007501

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti329 – 3335LDPIG → SGSYR in AAD26813. 1 Publication
Sequence conflicti530 – 5301L → V in CAA06329. 1 Publication
Sequence conflicti547 – 5482EV → DL in AAD26813. 1 Publication
Sequence conflicti595 – 5951N → T in AAH26823. 1 Publication
Sequence conflicti625 – 6251L → V in AAD26813. 1 Publication
Sequence conflicti640 – 6412KQ → NE in AAD26813. 1 Publication
Sequence conflicti821 – 8211G → R in AAD26813. 1 Publication
Sequence conflicti853 – 8531P → L in CAA06330. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ005073 mRNA. Translation: CAA06329.1.
AJ005074 mRNA. Translation: CAA06330.1.
AF119955 mRNA. Translation: AAD26813.1.
AK031256 mRNA. Translation: BAC27323.1.
AK167574 mRNA. Translation: BAE39637.1.
AK169704 mRNA. Translation: BAE41316.1.
CT025751, AC162177, AC167246 Genomic DNA. Translation: CAX15748.1.
CH466621 Genomic DNA. Translation: EDL08950.1.
BC002261 mRNA. Translation: AAH02261.1.
BC026823 mRNA. Translation: AAH26823.1.
AF176514 mRNA. Translation: AAD53115.1.
CCDSiCCDS23588.1. [Q9WU78-1]
CCDS52947.1. [Q9WU78-3]
RefSeqiNP_001158149.1. NM_001164677.1. [Q9WU78-3]
NP_001158150.1. NM_001164678.1.
NP_035182.2. NM_011052.2. [Q9WU78-1]
UniGeneiMm.29816.

Genome annotation databases

EnsembliENSMUST00000035086; ENSMUSP00000035086; ENSMUSG00000032504. [Q9WU78-1]
ENSMUST00000111861; ENSMUSP00000107492; ENSMUSG00000032504. [Q9WU78-3]
GeneIDi18571.
KEGGimmu:18571.
UCSCiuc009rwn.2. mouse. [Q9WU78-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ005073 mRNA. Translation: CAA06329.1 .
AJ005074 mRNA. Translation: CAA06330.1 .
AF119955 mRNA. Translation: AAD26813.1 .
AK031256 mRNA. Translation: BAC27323.1 .
AK167574 mRNA. Translation: BAE39637.1 .
AK169704 mRNA. Translation: BAE41316.1 .
CT025751 , AC162177 , AC167246 Genomic DNA. Translation: CAX15748.1 .
CH466621 Genomic DNA. Translation: EDL08950.1 .
BC002261 mRNA. Translation: AAH02261.1 .
BC026823 mRNA. Translation: AAH26823.1 .
AF176514 mRNA. Translation: AAD53115.1 .
CCDSi CCDS23588.1. [Q9WU78-1 ]
CCDS52947.1. [Q9WU78-3 ]
RefSeqi NP_001158149.1. NM_001164677.1. [Q9WU78-3 ]
NP_001158150.1. NM_001164678.1.
NP_035182.2. NM_011052.2. [Q9WU78-1 ]
UniGenei Mm.29816.

3D structure databases

ProteinModelPortali Q9WU78.
SMRi Q9WU78. Positions 2-698.
ModBasei Search...

Protein-protein interaction databases

BioGridi 202072. 5 interactions.
DIPi DIP-41418N.
IntActi Q9WU78. 10 interactions.
MINTi MINT-238635.

PTM databases

PhosphoSitei Q9WU78.

2D gel databases

REPRODUCTION-2DPAGE Q9WU78.
UCD-2DPAGE Q9WU78.

Proteomic databases

MaxQBi Q9WU78.
PaxDbi Q9WU78.
PRIDEi Q9WU78.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000035086 ; ENSMUSP00000035086 ; ENSMUSG00000032504 . [Q9WU78-1 ]
ENSMUST00000111861 ; ENSMUSP00000107492 ; ENSMUSG00000032504 . [Q9WU78-3 ]
GeneIDi 18571.
KEGGi mmu:18571.
UCSCi uc009rwn.2. mouse. [Q9WU78-1 ]

Organism-specific databases

CTDi 10015.
MGIi MGI:1333753. Pdcd6ip.

Phylogenomic databases

eggNOGi NOG325528.
GeneTreei ENSGT00750000117459.
HOVERGENi HBG053533.
KOi K12200.
OMAi QVKECYQ.
OrthoDBi EOG7V49XV.
TreeFami TF323502.

Miscellaneous databases

ChiTaRSi PDCD6IP. mouse.
NextBioi 294408.
PROi Q9WU78.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9WU78.
Bgeei Q9WU78.
CleanExi MM_PDCD6IP.
Genevestigatori Q9WU78.

Family and domain databases

Gene3Di 1.25.40.280. 1 hit.
InterProi IPR025304. ALIX_V_dom.
IPR004328. BRO1_dom.
[Graphical view ]
Pfami PF13949. ALIX_LYPXL_bnd. 1 hit.
PF03097. BRO1. 1 hit.
[Graphical view ]
SMARTi SM01041. BRO1. 1 hit.
[Graphical view ]
PROSITEi PS51180. BRO1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Alix, a novel mouse protein undergoing calcium-dependent interaction with the apoptotsis-linked-gene 2 (ALG-2) protein."
    Missotten M., Nichols A., Rieger K., Sadoul R.
    Cell Death Differ. 6:124-129(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), INTERACTION WITH PDCD6.
    Tissue: Brain.
  2. "Cloning of AIP1, a novel protein that associates with the apoptosis-linked gene ALG-2 in a Ca2+-dependent reaction."
    Vito P., Pellegrini L., Guiet C., D'Adamio L.
    J. Biol. Chem. 274:1533-1540(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Strain: C57BL/6J and NOD.
    Tissue: Forelimb, Placenta and Thymus.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Mammary gland.
  7. "Rapid analysis of gene expression (RAGE) facilitates universal expression profiling."
    Wang A.J., Pierce A., Judson-Kremer K., Gaddis S., Aldaz C.M., Johnson D.G., MacLeod M.C.
    Nucleic Acids Res. 27:4609-4618(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 671-869.
    Strain: C57BL/6J.
    Tissue: Forelimb.

Entry informationi

Entry nameiPDC6I_MOUSE
AccessioniPrimary (citable) accession number: Q9WU78
Secondary accession number(s): O88695
, O89014, Q3TED2, Q8BSL8, Q8R0H5, Q99LR3, Q9QZN8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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