Q9WU72 (TN13B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tumor necrosis factor ligand superfamily member 13B

Alternative name(s):
B-cell-activating factor
BAFF
CD_antigen=CD257

Cleaved into the following 2 chains:

1. Tumor necrosis factor ligand superfamily member 13b, membrane form
2. Tumor necrosis factor ligand superfamily member 13b, soluble form

Gene names

Name:	Tnfsf13b
Synonyms:	Baff

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. Ref.3 Isoform 2 seems to inhibit isoform 1 secretion and bioactivity. Ref.3
Subunit structure	Homotrimer. Isoform 2 heteromultimerizes with isoform 1, probably limiting the amount of functional isoform 1 on the cell surface. Ref.3
Subcellular location	Cell membrane; Single-pass type II membrane protein. Tumor necrosis factor ligand superfamily member 13b, soluble form: Secreted.
Tissue specificity	Isoform 2 is expressed in many myeloid cell lines. Ref.3
Post-translational modification	The soluble form derives from the membrane form by proteolytic processing. Isoform 2 is not efficiently shed from the membrane unlike isoform 1.
Sequence similarities	Belongs to the tumor necrosis factor family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Cytokine
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	B cell costimulation Inferred from direct assay. Source: MGI B cell homeostasis Inferred from mutant phenotype. Source: MGI T cell costimulation Inferred from direct assay. Source: MGI immune response Inferred from electronic annotation. Source: InterPro immunoglobulin secretion Inferred from genetic interaction. Source: MGI positive regulation of B cell proliferation Inferred from direct assay. Source: MGI positive regulation of T cell proliferation Inferred from direct assay. Source: MGI positive regulation of germinal center formation Inferred from mutant phenotype. Source: MGI positive regulation of survival gene product expression Inferred from direct assay. Source: MGI
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cytokine activity Inferred from electronic annotation. Source: UniProtKB-KW tumor necrosis factor receptor binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9WU72-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9WU72-2)

Also known as: DeltaBAFF;

The sequence of this isoform differs from the canonical sequence as follows:
     166-185: IIQDCLQLIADSDTPTIRKG → R

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Tumor necrosis factor ligand superfamily member 13b, membrane form		PRO_0000034530
	Chain	127 – 309	183	Tumor necrosis factor ligand superfamily member 13b, soluble form		PRO_0000034531
Regions
	Topological domain	1 – 47	47	Cytoplasmic Potential
	Transmembrane	48 – 68	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	69 – 309	241	Extracellular Potential
Sites
	Site	126 – 127	2	Cleavage By similarity
Amino acid modifications
	Glycosylation	117	1	N-linked (GlcNAc...) Potential
	Glycosylation	266	1	N-linked (GlcNAc...) Potential
	Disulfide bond	256 ↔ 269		By similarity
Natural variations
	Alternative sequence	166 – 185	20	IIQDC…TIRKG → R in isoform 2.		VSP_041184
	Natural variant	79	1	N → S in strain: NZB. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: F3DE6056E66034B4	FASTA	309	34,192
	10 20 30 40 50 60 MDESAKTLPP PCLCFCSEKG EDMKVGYDPI TPQKEEGAWF GICRDGRLLA ATLLLALLSS 70 80 90 100 110 120 SFTAMSLYQL AALQADLMNL RMELQSYRGS ATPAAAGAPE LTAGVKLLTP AAPRPHNSSR 130 140 150 160 170 180 GHRNRRAFQG PEETEQDVDL SAPPAPCLPG CRHSQHDDNG MNLRNIIQDC LQLIADSDTP 190 200 210 220 230 240 TIRKGTYTFV PWLLSFKRGN ALEEKENKIV VRQTGYFFIY SQVLYTDPIF AMGHVIQRKK 250 260 270 280 290 300 VHVFGDELSL VTLFRCIQNM PKTLPNNSCY SAGIARLEEG DEIQLAIPRE NAQISRNGDD TFFGALKLL
	Isoform 2 (DeltaBAFF) [UniParc]. Checksum: BC289F9FF8187C9C	FASTA	290	32,166
	10 20 30 40 50 60 MDESAKTLPP PCLCFCSEKG EDMKVGYDPI TPQKEEGAWF GICRDGRLLA ATLLLALLSS 70 80 90 100 110 120 SFTAMSLYQL AALQADLMNL RMELQSYRGS ATPAAAGAPE LTAGVKLLTP AAPRPHNSSR 130 140 150 160 170 180 GHRNRRAFQG PEETEQDVDL SAPPAPCLPG CRHSQHDDNG MNLRNRTYTF VPWLLSFKRG 190 200 210 220 230 240 NALEEKENKI VVRQTGYFFI YSQVLYTDPI FAMGHVIQRK KVHVFGDELS LVTLFRCIQN 250 260 270 280 290 MPKTLPNNSC YSAGIARLEE GDEIQLAIPR ENAQISRNGD DTFFGALKLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth." Schneider P., MacKay F., Steiner V., Hofmann K., Bodmer J.-L., Holler N., Ambrose C., Lawton P., Bixler S., Acha-Orbea H., Valmori D., Romero P., Werner-Favre C., Zubler R.H., Browning J.L., Tschopp J. J. Exp. Med. 189:1747-1756(1999) [PubMed: 10359578] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Polymorphism and chromosomal mapping of the mouse gene for B-cell activating factor belonging to the tumor necrosis factor family (Baff) and association with the autoimmune phenotype." Jiang Y., Ohtsuji M., Abe M., Li N., Xiu Y., Wen X.S., Shirai T., Hirose S. Immunogenetics 53:810-813(2001) [PubMed: 11862414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT SER-79. Strain: NZB.
[3]	"DeltaBAFF, an alternate splice isoform that regulates receptor binding and biopresentation of the B cell survival cytokine, BAFF." Gavin A.L., Ait-Azzouzene D., Ware C.F., Nemazee D. J. Biol. Chem. 278:38220-38228(2003) [PubMed: 12867412] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, FUNCTION, POST-TRANSLATIONAL MODIFICATION, SUBUNIT. Strain: BALB/c.
[4]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF119383 mRNA. Translation: AAD22475.1. AF352245 mRNA. Translation: AAL83939.1. AY290823 mRNA. Translation: AAP82036.1. CH466566 Genomic DNA. Translation: EDL22044.1. BC106840 mRNA. Translation: AAI06841.1.
IPI	IPI00124038. IPI00987994.
RefSeq	NP_296371.1. NM_033622.1.
UniGene	Mm.28835.
3D structure databases
ProteinModelPortal	Q9WU72.
SMR	Q9WU72. Positions 166-309.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9WU72.
Proteomic databases
PRIDE	Q9WU72.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000033892; ENSMUSP00000033892; ENSMUSG00000031497. ENSMUST00000164814; ENSMUSP00000129091; ENSMUSG00000031497.
GeneID	24099.
KEGG	mmu:24099.
Organism-specific databases
CTD	10673.
MGI	MGI:1344376. Tnfsf13b.
Phylogenomic databases
GeneTree	ENSGT00530000063837.
HOGENOM	HBG715020.
HOVERGEN	HBG061472.
InParanoid	Q9WU72.
OMA	ILPQKES.
OrthoDB	EOG47M206.
PhylomeDB	Q9WU72.
Gene expression databases
ArrayExpress	Q9WU72.
Bgee	Q9WU72.
Genevestigator	Q9WU72.
GermOnline	ENSMUSG00000031497. Mus musculus.
Family and domain databases
InterPro	IPR006052. TNF. IPR008983. Tumour_necrosis_fac-like. [Graphical view]
Gene3D	G3DSA:2.60.120.40. Tumour_necrosis_fac-like. 1 hit.
KO	K05476.
Pfam	PF00229. TNF. 1 hit. [Graphical view]
SMART	SM00207. TNF. 1 hit. [Graphical view]
SUPFAM	SSF49842. TNF_like. 1 hit.
PROSITE	PS00251. TNF_1. False negative. PS50049. TNF_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	304105.
SOURCE	Search...

Entry information

Entry name

TN13B_MOUSE

Accession

Primary (citable) accession number: Q9WU72
Secondary accession number(s): Q7TQ58

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	November 1, 1999
Last modified:	January 25, 2012
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program