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Q9WU70

- STXB5_RAT

UniProt

Q9WU70 - STXB5_RAT

Protein

Syntaxin-binding protein 5

Gene

Stxbp5

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Inhibits translocation of GLUT4 from intracellular vesicles to the plasma membrane By similarity. Plays a regulatory role in calcium-dependent exocytosis and neurotransmitter release. Inhibits membrane fusion between transport vesicles and the plasma membrane. May modulate the assembly of trans-SNARE complexes between transport vesicles and the plasma membrane. Competes with STXBP1 for STX1 binding.By similarity1 Publication

    GO - Molecular functioni

    1. syntaxin-1 binding Source: RGD

    GO - Biological processi

    1. exocytosis Source: UniProtKB-KW
    2. positive regulation of exocytosis Source: BHF-UCL
    3. protein transport Source: UniProtKB-KW

    Keywords - Biological processi

    Exocytosis, Protein transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Syntaxin-binding protein 5
    Alternative name(s):
    Lethal(2) giant larvae protein homolog 3
    Tomosyn-1
    Gene namesi
    Name:Stxbp5
    Synonyms:Llgl3
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi708517. Stxbp5.

    Subcellular locationi

    Cytoplasm 1 Publication. Cell membrane 1 Publication; Peripheral membrane protein 1 Publication. Cytoplasmic vesicle membrane 1 Publication; Peripheral membrane protein 1 Publication. Cell junctionsynapse 1 Publication. Cytoplasmic vesiclesecretory vesiclesynaptic vesicle 1 Publication
    Note: Cytoplasmic, and associated with vesicular membranes and the plasma membrane. Detected at synapses and on synaptic vesicles.

    GO - Cellular componenti

    1. acetylcholine-gated channel complex Source: Ensembl
    2. cell junction Source: UniProtKB-KW
    3. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
    4. secretory granule Source: BHF-UCL
    5. synaptic vesicle Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Membrane, Synapse

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi724 – 7241S → A: Strongly reduces phosphorylation. 1 Publication
    Mutagenesisi724 – 7241S → D: Reduces interaction with STX1A. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11521152Syntaxin-binding protein 5PRO_0000051246Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei724 – 7241Phosphoserine; by PKA1 Publication
    Modified residuei760 – 7601PhosphoserineBy similarity
    Modified residuei763 – 7631PhosphothreonineBy similarity
    Modified residuei783 – 7831PhosphoserineBy similarity
    Modified residuei786 – 7861PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylation by PKA reduces interaction with STX1A and enhances synaptic neurotransmitter release.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9WU70.
    PRIDEiQ9WU70.

    PTM databases

    PhosphoSiteiQ9WU70.

    Expressioni

    Tissue specificityi

    Isoform 1 is detected in heart, brain, lung, liver, skeletal muscle, kidney and testis. Isoform 2 is detected in brain and in testis. Isoform 3 is detected in testis.2 Publications

    Gene expression databases

    GenevestigatoriQ9WU70.

    Interactioni

    Subunit structurei

    Part of a complex that contains STXBP5, STX4A and SNAP23 By similarity. Interacts with STX1A and STX4A via its v-SNARE homology domain. Part of a complex that contains STX1, STXBP5, SNAP25 and SYT1.By similarity3 Publications

    Protein-protein interaction databases

    BioGridi249498. 2 interactions.
    IntActiQ9WU70. 1 interaction.

    Structurei

    Secondary structure

    1
    1152
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi1089 – 114355

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1URQX-ray2.00A1086-1145[»]
    ProteinModelPortaliQ9WU70.
    SMRiQ9WU70. Positions 1088-1144.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9WU70.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati62 – 9534WD 1Add
    BLAST
    Repeati102 – 14140WD 2Add
    BLAST
    Repeati146 – 18237WD 3Add
    BLAST
    Repeati201 – 23535WD 4Add
    BLAST
    Repeati241 – 27333WD 5Add
    BLAST
    Repeati295 – 33743WD 6Add
    BLAST
    Repeati345 – 37935WD 7Add
    BLAST
    Repeati401 – 47878WD 8Add
    BLAST
    Repeati506 – 620115WD 9Add
    BLAST
    Repeati634 – 69663WD 10Add
    BLAST
    Repeati795 – 85258WD 11Add
    BLAST
    Repeati861 – 93575WD 12Add
    BLAST
    Repeati940 – 98445WD 13Add
    BLAST
    Repeati998 – 102124WD 14Add
    BLAST
    Domaini1087 – 114761v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi22 – 287Poly-Gln
    Compositional biasi585 – 5917Poly-Ser

    Sequence similaritiesi

    Belongs to the WD repeat L(2)GL family.Curated
    Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation
    Contains 14 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Repeat, WD repeat

    Phylogenomic databases

    eggNOGiNOG289584.
    GeneTreeiENSGT00390000000018.
    HOGENOMiHOG000110929.
    HOVERGENiHBG083064.
    InParanoidiQ9WU70.
    KOiK08518.
    OMAiRYTHDEA.
    OrthoDBiEOG7DVD96.
    PhylomeDBiQ9WU70.

    Family and domain databases

    Gene3Di2.130.10.10. 2 hits.
    InterProiIPR000664. Lethal2_giant.
    IPR013905. Lgl_C_dom.
    IPR013577. LLGL2.
    IPR001388. Synaptobrevin.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF08596. Lgl_C. 1 hit.
    PF08366. LLGL. 1 hit.
    PF00400. WD40. 2 hits.
    [Graphical view]
    PRINTSiPR00962. LETHAL2GIANT.
    SMARTiSM00320. WD40. 7 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 6 hits.
    PROSITEiPS50892. V_SNARE. 1 hit.
    PS00678. WD_REPEATS_1. 2 hits.
    PS50082. WD_REPEATS_2. 1 hit.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9WU70-1) [UniParc]FASTAAdd to Basket

    Also known as: B-tomosyn

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRKFNIRKVL DGLTAGSSSA SQQQQQQQHP PGNREPEIQE TLQSEHFQLC     50
    KTVRHGFPYQ PSALAFDPVQ KILAVGTQTG ALRLFGRPGV ECYCQHDSGA 100
    AVIQLQFLIN EGALVSALAD DTLHLWNLRQ KRPAVLHSLK FCRERVTFCH 150
    LPFQSKWLYV GTERGNIHIV NVESFTLSGY VIMWNKAIEL SSKSHPGPVV 200
    HISDNPMDEG KLLIGFESGT VVLWDLKSKK ADYRYTYDEA IHSVAWHHEG 250
    KQFICSHSDG TLTIWNVRSP TKPVQTITPH GKQLKDGKKP EPCKPILKVE 300
    FKTTRSGEPF IILSGGLSYD TVGRRPCLTV MHGKSTAVLE MDYSIVDFLT 350
    LCETPYPNDF QEPYAVVVLL EKDLVLIDLA QNGYPIFENP YPLSIHESPV 400
    TCCEYFADCP VDLIPALYSV GARQKRQGYS KKEWPINGGN WGLGAQSYPE 450
    IIITGHADGS IKFWDASAIT LQVLYKLKTS KVFEKSRNKD DRQNTDIVDE 500
    DPYAIQIISW CPESRMLCIA GVSAHVIIYR FSKQEVVTEV IPMLEVRLLY 550
    EINDVETPEG EQPPPLSTPV GSSTSQPIPP QSHPSTSSSS SDGLRDNVPC 600
    LKVKNSPLKQ SPGYQTELVI QLVWVGGEPP QQITSLALNS SYGLVVFGNS 650
    NGIAMVDYLQ KAVLLNLSTI ELYGSNDPYR REPRSPRKSR QPSGAGLCDI 700
    TEGTVVPEDR CKSPTSGSSS PHNSDDEQKV NNFIEKVKTQ SRKFSKMVAS 750
    DLAKMSRKLS LPTDLKPDLD VKDNSFSRSR SSSVTSIDKE SREAISALHF 800
    CETFTRKADS SPSPCLWVGT TVGTAFVITL NLPLGPEQRL LQPVIVSPSG 850
    TILRLKGAIL RMAFLDAAGC LMPPAYEPWT EHNVPEEKDE KEKLKKRRPV 900
    SVSPSSSQEI SENQYAVICS EKQAKVISLP TQNCAYKQNI TETSFVLRGD 950
    IVALSNSVCL ACFCANGHIM TFSLPSLRPL LDVYYLPLTN MRIARTFCFA 1000
    NSGQALYLVS PTEIQRLTYS QETCENLQEM LGELFTPVET PEAPNRGFFK 1050
    GLFGGGAQSL DREELFGESS SGKASRSLAQ HIPGPGGIEG VKGAASGVVG 1100
    ELARARLALD ERGQKLSDLE ERTAAMMSSA DSFSKHAHEM MLKYKDKKWY 1150
    QF 1152
    Length:1,152
    Mass (Da):127,659
    Last modified:November 1, 1999 - v1
    Checksum:i1C07CABD8421CBD5
    GO
    Isoform 2 (identifier: Q9WU70-2) [UniParc]FASTAAdd to Basket

    Also known as: M-tomosyn

    The sequence of this isoform differs from the canonical sequence as follows:
         717-752: Missing.

    Show »
    Length:1,116
    Mass (Da):123,638
    Checksum:i034B99A939A987EA
    GO
    Isoform 3 (identifier: Q9WU70-3) [UniParc]FASTAAdd to Basket

    Also known as: S-tomosyn

    The sequence of this isoform differs from the canonical sequence as follows:
         717-769: Missing.

    Show »
    Length:1,099
    Mass (Da):121,743
    Checksum:iB199B25F2FE117CE
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei717 – 76953Missing in isoform 3. 1 PublicationVSP_016476Add
    BLAST
    Alternative sequencei717 – 75236Missing in isoform 2. 2 PublicationsVSP_016477Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U92072 mRNA. Translation: AAD04756.1.
    AF118889 mRNA. Translation: AAD27818.1.
    AF118890 mRNA. Translation: AAD27819.1.
    PIRiT42213.
    RefSeqiNP_110470.1. NM_030843.2. [Q9WU70-2]
    NP_848035.1. NM_178345.1. [Q9WU70-1]
    NP_848036.1. NM_178346.1. [Q9WU70-3]
    UniGeneiRn.96029.

    Genome annotation databases

    EnsembliENSRNOT00000040559; ENSRNOP00000044738; ENSRNOG00000013351. [Q9WU70-2]
    ENSRNOT00000044778; ENSRNOP00000039502; ENSRNOG00000013351. [Q9WU70-3]
    ENSRNOT00000049699; ENSRNOP00000049322; ENSRNOG00000013351. [Q9WU70-1]
    GeneIDi81022.
    KEGGirno:81022.
    UCSCiRGD:708517. rat. [Q9WU70-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U92072 mRNA. Translation: AAD04756.1 .
    AF118889 mRNA. Translation: AAD27818.1 .
    AF118890 mRNA. Translation: AAD27819.1 .
    PIRi T42213.
    RefSeqi NP_110470.1. NM_030843.2. [Q9WU70-2 ]
    NP_848035.1. NM_178345.1. [Q9WU70-1 ]
    NP_848036.1. NM_178346.1. [Q9WU70-3 ]
    UniGenei Rn.96029.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1URQ X-ray 2.00 A 1086-1145 [» ]
    ProteinModelPortali Q9WU70.
    SMRi Q9WU70. Positions 1088-1144.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 249498. 2 interactions.
    IntActi Q9WU70. 1 interaction.

    PTM databases

    PhosphoSitei Q9WU70.

    Proteomic databases

    PaxDbi Q9WU70.
    PRIDEi Q9WU70.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000040559 ; ENSRNOP00000044738 ; ENSRNOG00000013351 . [Q9WU70-2 ]
    ENSRNOT00000044778 ; ENSRNOP00000039502 ; ENSRNOG00000013351 . [Q9WU70-3 ]
    ENSRNOT00000049699 ; ENSRNOP00000049322 ; ENSRNOG00000013351 . [Q9WU70-1 ]
    GeneIDi 81022.
    KEGGi rno:81022.
    UCSCi RGD:708517. rat. [Q9WU70-1 ]

    Organism-specific databases

    CTDi 134957.
    RGDi 708517. Stxbp5.

    Phylogenomic databases

    eggNOGi NOG289584.
    GeneTreei ENSGT00390000000018.
    HOGENOMi HOG000110929.
    HOVERGENi HBG083064.
    InParanoidi Q9WU70.
    KOi K08518.
    OMAi RYTHDEA.
    OrthoDBi EOG7DVD96.
    PhylomeDBi Q9WU70.

    Miscellaneous databases

    EvolutionaryTracei Q9WU70.
    NextBioi 614943.
    PROi Q9WU70.

    Gene expression databases

    Genevestigatori Q9WU70.

    Family and domain databases

    Gene3Di 2.130.10.10. 2 hits.
    InterProi IPR000664. Lethal2_giant.
    IPR013905. Lgl_C_dom.
    IPR013577. LLGL2.
    IPR001388. Synaptobrevin.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF08596. Lgl_C. 1 hit.
    PF08366. LLGL. 1 hit.
    PF00400. WD40. 2 hits.
    [Graphical view ]
    PRINTSi PR00962. LETHAL2GIANT.
    SMARTi SM00320. WD40. 7 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 6 hits.
    PROSITEi PS50892. V_SNARE. 1 hit.
    PS00678. WD_REPEATS_1. 2 hits.
    PS50082. WD_REPEATS_2. 1 hit.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Tomosyn: a syntaxin-1-binding protein that forms a novel complex in the neurotransmitter release process."
      Fujita Y., Shirataki H., Sakisaka T., Asakura T., Ohya T., Kotani H., Yokoyama S., Nishioka H., Matsuura Y., Mizoguchi A., Scheller R.H., Takai Y.
      Neuron 20:905-915(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), PARTIAL PROTEIN SEQUENCE, FUNCTION, INTERACTION WITH STX1A AND STX1B, IDENTIFICATION IN A COMPLEX WITH STX1A; SNAP25 AND SYT1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Tissue: Brain.
    2. "Three splicing variants of tomosyn and identification of their syntaxin-binding region."
      Yokoyama S., Shirataki H., Sakisaka T., Takai Y.
      Biochem. Biophys. Res. Commun. 256:218-222(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), INTERACTION WITH STX1A, TISSUE SPECIFICITY.
      Tissue: Kidney.
    3. "PKA-catalyzed phosphorylation of tomosyn and its implication in Ca2+-dependent exocytosis of neurotransmitter."
      Baba T., Sakisaka T., Mochida S., Takai Y.
      J. Cell Biol. 170:1113-1125(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF SER-724, PHOSPHORYLATION AT SER-724.
    4. "Structural basis for the inhibitory role of tomosyn in exocytosis."
      Pobbati A.V., Razeto A., Boeddener M., Becker S., Fasshauer D.
      J. Biol. Chem. 279:47192-47200(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1010-1145 IN COMPLEX WITH STX1A AND SNAP25.

    Entry informationi

    Entry nameiSTXB5_RAT
    AccessioniPrimary (citable) accession number: Q9WU70
    Secondary accession number(s): Q9WU71, Q9Z152
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3