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Protein

Syntaxin-binding protein 5

Gene

Stxbp5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits translocation of GLUT4 from intracellular vesicles to the plasma membrane (By similarity). Plays a regulatory role in calcium-dependent exocytosis and neurotransmitter release. Inhibits membrane fusion between transport vesicles and the plasma membrane. May modulate the assembly of trans-SNARE complexes between transport vesicles and the plasma membrane. Competes with STXBP1 for STX1 binding.By similarity1 Publication

GO - Molecular functioni

  • GTPase activator activity Source: GO_Central
  • Rab GTPase binding Source: GO_Central
  • syntaxin-1 binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Exocytosis, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin-binding protein 5
Alternative name(s):
Lethal(2) giant larvae protein homolog 3
Tomosyn-1
Gene namesi
Name:Stxbp5
Synonyms:Llgl3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi708517. Stxbp5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi724S → A: Strongly reduces phosphorylation. 1 Publication1
Mutagenesisi724S → D: Reduces interaction with STX1A. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000512461 – 1152Syntaxin-binding protein 5Add BLAST1152

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei693PhosphoserineBy similarity1
Modified residuei724Phosphoserine; by PKA1 Publication1
Modified residuei760PhosphoserineCombined sources1
Modified residuei763PhosphothreonineBy similarity1
Modified residuei783PhosphoserineCombined sources1
Modified residuei785PhosphothreonineBy similarity1
Modified residuei786PhosphoserineBy similarity1
Modified residuei901PhosphoserineBy similarity1
Modified residuei903PhosphoserineBy similarity1
Modified residuei1040PhosphothreonineBy similarity1
Modified residuei1059PhosphoserineBy similarity1
Modified residuei1132PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation by PKA reduces interaction with STX1A and enhances synaptic neurotransmitter release.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9WU70.
PRIDEiQ9WU70.

PTM databases

iPTMnetiQ9WU70.
PhosphoSitePlusiQ9WU70.
SwissPalmiQ9WU70.

Expressioni

Tissue specificityi

Isoform 1 is detected in heart, brain, lung, liver, skeletal muscle, kidney and testis. Isoform 2 is detected in brain and in testis. Isoform 3 is detected in testis.2 Publications

Gene expression databases

BgeeiENSRNOG00000013351.
GenevisibleiQ9WU70. RN.

Interactioni

Subunit structurei

Part of a complex that contains STXBP5, STX4A and SNAP23 (By similarity). Interacts with STX1A and STX4A via its v-SNARE homology domain. Part of a complex that contains STX1, STXBP5, SNAP25 and SYT1.By similarity3 Publications

GO - Molecular functioni

  • Rab GTPase binding Source: GO_Central
  • syntaxin-1 binding Source: RGD

Protein-protein interaction databases

BioGridi249498. 2 interactors.
IntActiQ9WU70. 1 interactor.
STRINGi10116.ENSRNOP00000049322.

Structurei

Secondary structure

11152
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1089 – 1143Combined sources55

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1URQX-ray2.00A1086-1145[»]
ProteinModelPortaliQ9WU70.
SMRiQ9WU70.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WU70.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati62 – 95WD 1Add BLAST34
Repeati102 – 141WD 2Add BLAST40
Repeati146 – 182WD 3Add BLAST37
Repeati201 – 235WD 4Add BLAST35
Repeati241 – 273WD 5Add BLAST33
Repeati295 – 337WD 6Add BLAST43
Repeati345 – 379WD 7Add BLAST35
Repeati401 – 478WD 8Add BLAST78
Repeati506 – 620WD 9Add BLAST115
Repeati634 – 696WD 10Add BLAST63
Repeati795 – 852WD 11Add BLAST58
Repeati861 – 935WD 12Add BLAST75
Repeati940 – 984WD 13Add BLAST45
Repeati998 – 1021WD 14Add BLAST24
Domaini1087 – 1147v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST61

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi22 – 28Poly-Gln7
Compositional biasi585 – 591Poly-Ser7

Sequence similaritiesi

Belongs to the WD repeat L(2)GL family.Curated
Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation
Contains 14 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410INFH. Eukaryota.
ENOG410XTER. LUCA.
GeneTreeiENSGT00390000000018.
HOGENOMiHOG000110929.
HOVERGENiHBG083064.
InParanoidiQ9WU70.
KOiK08518.
OMAiRYTHDEA.
OrthoDBiEOG091G0LY3.
PhylomeDBiQ9WU70.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR000664. Lethal2_giant.
IPR013905. Lgl_C_dom.
IPR013577. LLGL2.
IPR001388. Synaptobrevin.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08596. Lgl_C. 1 hit.
PF08366. LLGL. 1 hit.
PF00400. WD40. 1 hit.
[Graphical view]
PRINTSiPR00962. LETHAL2GIANT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 6 hits.
PROSITEiPS50892. V_SNARE. 1 hit.
PS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9WU70-1) [UniParc]FASTAAdd to basket
Also known as: B-tomosyn

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRKFNIRKVL DGLTAGSSSA SQQQQQQQHP PGNREPEIQE TLQSEHFQLC
60 70 80 90 100
KTVRHGFPYQ PSALAFDPVQ KILAVGTQTG ALRLFGRPGV ECYCQHDSGA
110 120 130 140 150
AVIQLQFLIN EGALVSALAD DTLHLWNLRQ KRPAVLHSLK FCRERVTFCH
160 170 180 190 200
LPFQSKWLYV GTERGNIHIV NVESFTLSGY VIMWNKAIEL SSKSHPGPVV
210 220 230 240 250
HISDNPMDEG KLLIGFESGT VVLWDLKSKK ADYRYTYDEA IHSVAWHHEG
260 270 280 290 300
KQFICSHSDG TLTIWNVRSP TKPVQTITPH GKQLKDGKKP EPCKPILKVE
310 320 330 340 350
FKTTRSGEPF IILSGGLSYD TVGRRPCLTV MHGKSTAVLE MDYSIVDFLT
360 370 380 390 400
LCETPYPNDF QEPYAVVVLL EKDLVLIDLA QNGYPIFENP YPLSIHESPV
410 420 430 440 450
TCCEYFADCP VDLIPALYSV GARQKRQGYS KKEWPINGGN WGLGAQSYPE
460 470 480 490 500
IIITGHADGS IKFWDASAIT LQVLYKLKTS KVFEKSRNKD DRQNTDIVDE
510 520 530 540 550
DPYAIQIISW CPESRMLCIA GVSAHVIIYR FSKQEVVTEV IPMLEVRLLY
560 570 580 590 600
EINDVETPEG EQPPPLSTPV GSSTSQPIPP QSHPSTSSSS SDGLRDNVPC
610 620 630 640 650
LKVKNSPLKQ SPGYQTELVI QLVWVGGEPP QQITSLALNS SYGLVVFGNS
660 670 680 690 700
NGIAMVDYLQ KAVLLNLSTI ELYGSNDPYR REPRSPRKSR QPSGAGLCDI
710 720 730 740 750
TEGTVVPEDR CKSPTSGSSS PHNSDDEQKV NNFIEKVKTQ SRKFSKMVAS
760 770 780 790 800
DLAKMSRKLS LPTDLKPDLD VKDNSFSRSR SSSVTSIDKE SREAISALHF
810 820 830 840 850
CETFTRKADS SPSPCLWVGT TVGTAFVITL NLPLGPEQRL LQPVIVSPSG
860 870 880 890 900
TILRLKGAIL RMAFLDAAGC LMPPAYEPWT EHNVPEEKDE KEKLKKRRPV
910 920 930 940 950
SVSPSSSQEI SENQYAVICS EKQAKVISLP TQNCAYKQNI TETSFVLRGD
960 970 980 990 1000
IVALSNSVCL ACFCANGHIM TFSLPSLRPL LDVYYLPLTN MRIARTFCFA
1010 1020 1030 1040 1050
NSGQALYLVS PTEIQRLTYS QETCENLQEM LGELFTPVET PEAPNRGFFK
1060 1070 1080 1090 1100
GLFGGGAQSL DREELFGESS SGKASRSLAQ HIPGPGGIEG VKGAASGVVG
1110 1120 1130 1140 1150
ELARARLALD ERGQKLSDLE ERTAAMMSSA DSFSKHAHEM MLKYKDKKWY

QF
Length:1,152
Mass (Da):127,659
Last modified:November 1, 1999 - v1
Checksum:i1C07CABD8421CBD5
GO
Isoform 2 (identifier: Q9WU70-2) [UniParc]FASTAAdd to basket
Also known as: M-tomosyn

The sequence of this isoform differs from the canonical sequence as follows:
     717-752: Missing.

Show »
Length:1,116
Mass (Da):123,638
Checksum:i034B99A939A987EA
GO
Isoform 3 (identifier: Q9WU70-3) [UniParc]FASTAAdd to basket
Also known as: S-tomosyn

The sequence of this isoform differs from the canonical sequence as follows:
     717-769: Missing.

Show »
Length:1,099
Mass (Da):121,743
Checksum:iB199B25F2FE117CE
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016476717 – 769Missing in isoform 3. 1 PublicationAdd BLAST53
Alternative sequenceiVSP_016477717 – 752Missing in isoform 2. 2 PublicationsAdd BLAST36

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92072 mRNA. Translation: AAD04756.1.
AF118889 mRNA. Translation: AAD27818.1.
AF118890 mRNA. Translation: AAD27819.1.
PIRiT42213.
RefSeqiNP_110470.1. NM_030843.2. [Q9WU70-2]
NP_848035.1. NM_178345.1. [Q9WU70-1]
NP_848036.1. NM_178346.1. [Q9WU70-3]
UniGeneiRn.96029.

Genome annotation databases

EnsembliENSRNOT00000040559; ENSRNOP00000044738; ENSRNOG00000013351. [Q9WU70-2]
ENSRNOT00000044778; ENSRNOP00000039502; ENSRNOG00000013351. [Q9WU70-3]
ENSRNOT00000049699; ENSRNOP00000049322; ENSRNOG00000013351. [Q9WU70-1]
GeneIDi81022.
KEGGirno:81022.
UCSCiRGD:708517. rat. [Q9WU70-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92072 mRNA. Translation: AAD04756.1.
AF118889 mRNA. Translation: AAD27818.1.
AF118890 mRNA. Translation: AAD27819.1.
PIRiT42213.
RefSeqiNP_110470.1. NM_030843.2. [Q9WU70-2]
NP_848035.1. NM_178345.1. [Q9WU70-1]
NP_848036.1. NM_178346.1. [Q9WU70-3]
UniGeneiRn.96029.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1URQX-ray2.00A1086-1145[»]
ProteinModelPortaliQ9WU70.
SMRiQ9WU70.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249498. 2 interactors.
IntActiQ9WU70. 1 interactor.
STRINGi10116.ENSRNOP00000049322.

PTM databases

iPTMnetiQ9WU70.
PhosphoSitePlusiQ9WU70.
SwissPalmiQ9WU70.

Proteomic databases

PaxDbiQ9WU70.
PRIDEiQ9WU70.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000040559; ENSRNOP00000044738; ENSRNOG00000013351. [Q9WU70-2]
ENSRNOT00000044778; ENSRNOP00000039502; ENSRNOG00000013351. [Q9WU70-3]
ENSRNOT00000049699; ENSRNOP00000049322; ENSRNOG00000013351. [Q9WU70-1]
GeneIDi81022.
KEGGirno:81022.
UCSCiRGD:708517. rat. [Q9WU70-1]

Organism-specific databases

CTDi134957.
RGDi708517. Stxbp5.

Phylogenomic databases

eggNOGiENOG410INFH. Eukaryota.
ENOG410XTER. LUCA.
GeneTreeiENSGT00390000000018.
HOGENOMiHOG000110929.
HOVERGENiHBG083064.
InParanoidiQ9WU70.
KOiK08518.
OMAiRYTHDEA.
OrthoDBiEOG091G0LY3.
PhylomeDBiQ9WU70.

Miscellaneous databases

EvolutionaryTraceiQ9WU70.
PROiQ9WU70.

Gene expression databases

BgeeiENSRNOG00000013351.
GenevisibleiQ9WU70. RN.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR000664. Lethal2_giant.
IPR013905. Lgl_C_dom.
IPR013577. LLGL2.
IPR001388. Synaptobrevin.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08596. Lgl_C. 1 hit.
PF08366. LLGL. 1 hit.
PF00400. WD40. 1 hit.
[Graphical view]
PRINTSiPR00962. LETHAL2GIANT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 6 hits.
PROSITEiPS50892. V_SNARE. 1 hit.
PS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTXB5_RAT
AccessioniPrimary (citable) accession number: Q9WU70
Secondary accession number(s): Q9WU71, Q9Z152
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.