Q9WU70 (STXB5_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Syntaxin-binding protein 5

Alternative name(s):
Lethal(2) giant larvae protein homolog 3
Tomosyn-1

Gene names

Name:	Stxbp5
Synonyms:	Llgl3

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1152 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits translocation of GLUT4 from intracellular vesicles to the plasma membrane By similarity. Plays a regulatory role in calcium-dependent exocytosis and neurotransmitter release. Inhibits membrane fusion between transport vesicles and the plasma membrane. May modulate the assembly of trans-SNARE complexes between transport vesicles and the plasma membrane. Competes with STXBP1 for STX1 binding. Ref.1
Subunit structure	Part of a complex that contains STXBP5, STX4A and SNAP23 By similarity. Interacts with STX1A and STX4A via its v-SNARE homology domain. Part of a complex that contains STX1, STXBP5, SNAP25 and SYT1. Ref.1 Ref.2
Subcellular location	Cytoplasm. Cell membrane; Peripheral membrane protein. Cytoplasmic vesicle membrane; Peripheral membrane protein. Cell junction › synapse. Cytoplasmic vesicle › secretory vesicle › synaptic vesicle. Note: Cytoplasmic, and associated with vesicular membranes and the plasma membrane. Detected at synapses and on synaptic vesicles. Ref.1
Tissue specificity	Isoform 1 is detected in heart, brain, lung, liver, skeletal muscle, kidney and testis. Isoform 2 is detected in brain and in testis. Isoform 3 is detected in testis. Ref.1 Ref.2
Post-translational modification	Phosphorylation by PKA reduces interaction with STX1A and enhances synaptic neurotransmitter release.
Sequence similarities	Belongs to the WD repeat L(2)GL family. Contains 1 v-SNARE coiled-coil homology domain. Contains 14 WD repeats.

Ontologies

Keywords
Biological process	Exocytosis Protein transport Transport
Cellular component	Cell junction Cell membrane Cytoplasm Cytoplasmic vesicle Membrane Synapse
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Repeat WD repeat
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	exocytosis Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of exocytosis Traceable author statement PubMed 16787939. Source: BHF-UCL protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	acetylcholine-gated channel complex Inferred from electronic annotation. Source: Compara cell junction Inferred from electronic annotation. Source: UniProtKB-KW cytoplasmic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell secretory granule Traceable author statement PubMed 16787939. Source: BHF-UCL synaptic vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	syntaxin-1 binding Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9WU70-1)

Also known as: B-tomosyn;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9WU70-2)

Also known as: M-tomosyn;

The sequence of this isoform differs from the canonical sequence as follows:
     717-752: Missing.

Isoform 3 (identifier: Q9WU70-3)

Also known as: S-tomosyn;

The sequence of this isoform differs from the canonical sequence as follows:
     717-769: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1152

1152

Syntaxin-binding protein 5

PRO_0000051246

Regions

Repeat

62 – 95

34

WD 1

Repeat

102 – 141

40

WD 2

Repeat

146 – 182

37

WD 3

Repeat

201 – 235

35

WD 4

Repeat

241 – 273

33

WD 5

Repeat

295 – 337

43

WD 6

Repeat

345 – 379

35

WD 7

Repeat

401 – 478

78

WD 8

Repeat

506 – 620

115

WD 9

Repeat

634 – 696

63

WD 10

Repeat

795 – 852

58

WD 11

Repeat

861 – 935

75

WD 12

Repeat

940 – 984

45

WD 13

Repeat

998 – 1021

24

WD 14

Domain

1087 – 1147

61

v-SNARE coiled-coil homology

Compositional bias

22 – 28

7

Poly-Gln

Compositional bias

585 – 591

7

Poly-Ser

Amino acid modifications

Modified residue

724

1

Phosphoserine; by PKA Ref.3

Modified residue

760

1

Phosphoserine By similarity

Modified residue

763

1

Phosphothreonine By similarity

Modified residue

783

1

Phosphoserine By similarity

Modified residue

786

1

Phosphoserine By similarity

Modified residue

1059

1

Phosphoserine By similarity

Natural variations

Alternative sequence

717 – 769

53

Missing in isoform 3.

VSP_016476

Alternative sequence

717 – 752

36

Missing in isoform 2.

VSP_016477

Experimental info

Mutagenesis

724

1

S → A: Strongly reduces phosphorylation. Ref.3

Mutagenesis

724

1

S → D: Reduces interaction with STX1A. Ref.3

Secondary structure

1

.

.

.

1152

Helix Strand Turn

Details...

Helix

1089 – 1143

55

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (B-tomosyn) [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 1C07CABD8421CBD5	FASTA	1,152	127,659
	10 20 30 40 50 60 MRKFNIRKVL DGLTAGSSSA SQQQQQQQHP PGNREPEIQE TLQSEHFQLC KTVRHGFPYQ 70 80 90 100 110 120 PSALAFDPVQ KILAVGTQTG ALRLFGRPGV ECYCQHDSGA AVIQLQFLIN EGALVSALAD 130 140 150 160 170 180 DTLHLWNLRQ KRPAVLHSLK FCRERVTFCH LPFQSKWLYV GTERGNIHIV NVESFTLSGY 190 200 210 220 230 240 VIMWNKAIEL SSKSHPGPVV HISDNPMDEG KLLIGFESGT VVLWDLKSKK ADYRYTYDEA 250 260 270 280 290 300 IHSVAWHHEG KQFICSHSDG TLTIWNVRSP TKPVQTITPH GKQLKDGKKP EPCKPILKVE 310 320 330 340 350 360 FKTTRSGEPF IILSGGLSYD TVGRRPCLTV MHGKSTAVLE MDYSIVDFLT LCETPYPNDF 370 380 390 400 410 420 QEPYAVVVLL EKDLVLIDLA QNGYPIFENP YPLSIHESPV TCCEYFADCP VDLIPALYSV 430 440 450 460 470 480 GARQKRQGYS KKEWPINGGN WGLGAQSYPE IIITGHADGS IKFWDASAIT LQVLYKLKTS 490 500 510 520 530 540 KVFEKSRNKD DRQNTDIVDE DPYAIQIISW CPESRMLCIA GVSAHVIIYR FSKQEVVTEV 550 560 570 580 590 600 IPMLEVRLLY EINDVETPEG EQPPPLSTPV GSSTSQPIPP QSHPSTSSSS SDGLRDNVPC 610 620 630 640 650 660 LKVKNSPLKQ SPGYQTELVI QLVWVGGEPP QQITSLALNS SYGLVVFGNS NGIAMVDYLQ 670 680 690 700 710 720 KAVLLNLSTI ELYGSNDPYR REPRSPRKSR QPSGAGLCDI TEGTVVPEDR CKSPTSGSSS 730 740 750 760 770 780 PHNSDDEQKV NNFIEKVKTQ SRKFSKMVAS DLAKMSRKLS LPTDLKPDLD VKDNSFSRSR 790 800 810 820 830 840 SSSVTSIDKE SREAISALHF CETFTRKADS SPSPCLWVGT TVGTAFVITL NLPLGPEQRL 850 860 870 880 890 900 LQPVIVSPSG TILRLKGAIL RMAFLDAAGC LMPPAYEPWT EHNVPEEKDE KEKLKKRRPV 910 920 930 940 950 960 SVSPSSSQEI SENQYAVICS EKQAKVISLP TQNCAYKQNI TETSFVLRGD IVALSNSVCL 970 980 990 1000 1010 1020 ACFCANGHIM TFSLPSLRPL LDVYYLPLTN MRIARTFCFA NSGQALYLVS PTEIQRLTYS 1030 1040 1050 1060 1070 1080 QETCENLQEM LGELFTPVET PEAPNRGFFK GLFGGGAQSL DREELFGESS SGKASRSLAQ 1090 1100 1110 1120 1130 1140 HIPGPGGIEG VKGAASGVVG ELARARLALD ERGQKLSDLE ERTAAMMSSA DSFSKHAHEM 1150 MLKYKDKKWY QF
	Isoform 2 (M-tomosyn) [UniParc]. Checksum: 034B99A939A987EA	FASTA	1,116	123,638
	10 20 30 40 50 60 MRKFNIRKVL DGLTAGSSSA SQQQQQQQHP PGNREPEIQE TLQSEHFQLC KTVRHGFPYQ 70 80 90 100 110 120 PSALAFDPVQ KILAVGTQTG ALRLFGRPGV ECYCQHDSGA AVIQLQFLIN EGALVSALAD 130 140 150 160 170 180 DTLHLWNLRQ KRPAVLHSLK FCRERVTFCH LPFQSKWLYV GTERGNIHIV NVESFTLSGY 190 200 210 220 230 240 VIMWNKAIEL SSKSHPGPVV HISDNPMDEG KLLIGFESGT VVLWDLKSKK ADYRYTYDEA 250 260 270 280 290 300 IHSVAWHHEG KQFICSHSDG TLTIWNVRSP TKPVQTITPH GKQLKDGKKP EPCKPILKVE 310 320 330 340 350 360 FKTTRSGEPF IILSGGLSYD TVGRRPCLTV MHGKSTAVLE MDYSIVDFLT LCETPYPNDF 370 380 390 400 410 420 QEPYAVVVLL EKDLVLIDLA QNGYPIFENP YPLSIHESPV TCCEYFADCP VDLIPALYSV 430 440 450 460 470 480 GARQKRQGYS KKEWPINGGN WGLGAQSYPE IIITGHADGS IKFWDASAIT LQVLYKLKTS 490 500 510 520 530 540 KVFEKSRNKD DRQNTDIVDE DPYAIQIISW CPESRMLCIA GVSAHVIIYR FSKQEVVTEV 550 560 570 580 590 600 IPMLEVRLLY EINDVETPEG EQPPPLSTPV GSSTSQPIPP QSHPSTSSSS SDGLRDNVPC 610 620 630 640 650 660 LKVKNSPLKQ SPGYQTELVI QLVWVGGEPP QQITSLALNS SYGLVVFGNS NGIAMVDYLQ 670 680 690 700 710 720 KAVLLNLSTI ELYGSNDPYR REPRSPRKSR QPSGAGLCDI TEGTVVPEDR CKSPTSAKMS 730 740 750 760 770 780 RKLSLPTDLK PDLDVKDNSF SRSRSSSVTS IDKESREAIS ALHFCETFTR KADSSPSPCL 790 800 810 820 830 840 WVGTTVGTAF VITLNLPLGP EQRLLQPVIV SPSGTILRLK GAILRMAFLD AAGCLMPPAY 850 860 870 880 890 900 EPWTEHNVPE EKDEKEKLKK RRPVSVSPSS SQEISENQYA VICSEKQAKV ISLPTQNCAY 910 920 930 940 950 960 KQNITETSFV LRGDIVALSN SVCLACFCAN GHIMTFSLPS LRPLLDVYYL PLTNMRIART 970 980 990 1000 1010 1020 FCFANSGQAL YLVSPTEIQR LTYSQETCEN LQEMLGELFT PVETPEAPNR GFFKGLFGGG 1030 1040 1050 1060 1070 1080 AQSLDREELF GESSSGKASR SLAQHIPGPG GIEGVKGAAS GVVGELARAR LALDERGQKL 1090 1100 1110 SDLEERTAAM MSSADSFSKH AHEMMLKYKD KKWYQF
	Isoform 3 (S-tomosyn) [UniParc]. Checksum: B199B25F2FE117CE	FASTA	1,099	121,743
	10 20 30 40 50 60 MRKFNIRKVL DGLTAGSSSA SQQQQQQQHP PGNREPEIQE TLQSEHFQLC KTVRHGFPYQ 70 80 90 100 110 120 PSALAFDPVQ KILAVGTQTG ALRLFGRPGV ECYCQHDSGA AVIQLQFLIN EGALVSALAD 130 140 150 160 170 180 DTLHLWNLRQ KRPAVLHSLK FCRERVTFCH LPFQSKWLYV GTERGNIHIV NVESFTLSGY 190 200 210 220 230 240 VIMWNKAIEL SSKSHPGPVV HISDNPMDEG KLLIGFESGT VVLWDLKSKK ADYRYTYDEA 250 260 270 280 290 300 IHSVAWHHEG KQFICSHSDG TLTIWNVRSP TKPVQTITPH GKQLKDGKKP EPCKPILKVE 310 320 330 340 350 360 FKTTRSGEPF IILSGGLSYD TVGRRPCLTV MHGKSTAVLE MDYSIVDFLT LCETPYPNDF 370 380 390 400 410 420 QEPYAVVVLL EKDLVLIDLA QNGYPIFENP YPLSIHESPV TCCEYFADCP VDLIPALYSV 430 440 450 460 470 480 GARQKRQGYS KKEWPINGGN WGLGAQSYPE IIITGHADGS IKFWDASAIT LQVLYKLKTS 490 500 510 520 530 540 KVFEKSRNKD DRQNTDIVDE DPYAIQIISW CPESRMLCIA GVSAHVIIYR FSKQEVVTEV 550 560 570 580 590 600 IPMLEVRLLY EINDVETPEG EQPPPLSTPV GSSTSQPIPP QSHPSTSSSS SDGLRDNVPC 610 620 630 640 650 660 LKVKNSPLKQ SPGYQTELVI QLVWVGGEPP QQITSLALNS SYGLVVFGNS NGIAMVDYLQ 670 680 690 700 710 720 KAVLLNLSTI ELYGSNDPYR REPRSPRKSR QPSGAGLCDI TEGTVVPEDR CKSPTSDVKD 730 740 750 760 770 780 NSFSRSRSSS VTSIDKESRE AISALHFCET FTRKADSSPS PCLWVGTTVG TAFVITLNLP 790 800 810 820 830 840 LGPEQRLLQP VIVSPSGTIL RLKGAILRMA FLDAAGCLMP PAYEPWTEHN VPEEKDEKEK 850 860 870 880 890 900 LKKRRPVSVS PSSSQEISEN QYAVICSEKQ AKVISLPTQN CAYKQNITET SFVLRGDIVA 910 920 930 940 950 960 LSNSVCLACF CANGHIMTFS LPSLRPLLDV YYLPLTNMRI ARTFCFANSG QALYLVSPTE 970 980 990 1000 1010 1020 IQRLTYSQET CENLQEMLGE LFTPVETPEA PNRGFFKGLF GGGAQSLDRE ELFGESSSGK 1030 1040 1050 1060 1070 1080 ASRSLAQHIP GPGGIEGVKG AASGVVGELA RARLALDERG QKLSDLEERT AAMMSSADSF 1090 SKHAHEMMLK YKDKKWYQF

References

[1]	"Tomosyn: a syntaxin-1-binding protein that forms a novel complex in the neurotransmitter release process." Fujita Y., Shirataki H., Sakisaka T., Asakura T., Ohya T., Kotani H., Yokoyama S., Nishioka H., Matsuura Y., Mizoguchi A., Scheller R.H., Takai Y. Neuron 20:905-915(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), PARTIAL PROTEIN SEQUENCE, FUNCTION, INTERACTION WITH STX1A AND STX1B, IDENTIFICATION IN A COMPLEX WITH STX1A; SNAP25 AND SYT1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Brain.
[2]	"Three splicing variants of tomosyn and identification of their syntaxin-binding region." Yokoyama S., Shirataki H., Sakisaka T., Takai Y. Biochem. Biophys. Res. Commun. 256:218-222(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), INTERACTION WITH STX1A, TISSUE SPECIFICITY. Tissue: Kidney.
[3]	"PKA-catalyzed phosphorylation of tomosyn and its implication in Ca2+-dependent exocytosis of neurotransmitter." Baba T., Sakisaka T., Mochida S., Takai Y. J. Cell Biol. 170:1113-1125(2005) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF SER-724, PHOSPHORYLATION AT SER-724.
[4]	"Structural basis for the inhibitory role of tomosyn in exocytosis." Pobbati A.V., Razeto A., Boeddener M., Becker S., Fasshauer D. J. Biol. Chem. 279:47192-47200(2004) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1010-1145 IN COMPLEX WITH STX1A AND SNAP25.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U92072 mRNA. Translation: AAD04756.1.
AF118889 mRNA. Translation: AAD27818.1.
AF118890 mRNA. Translation: AAD27819.1.

IPI

IPI00203457.
IPI00212278.
IPI00564719.

PIR

T42213.

RefSeq

NP_110470.1. NM_030843.1.
NP_848035.1. NM_178345.1.
NP_848036.1. NM_178346.1.

UniGene

Rn.96029.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1URQ	X-ray	2.00	A	1086-1145	[»]

ProteinModelPortal

Q9WU70.

SMR

Q9WU70. Positions 1088-1144.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9WU70. 1 interaction.

PTM databases

PhosphoSite

Q9WU70.

Proteomic databases

PaxDb

Q9WU70.

PRIDE

Q9WU70.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSRNOT00000040559; ENSRNOP00000044738; ENSRNOG00000013351.
ENSRNOT00000044778; ENSRNOP00000039502; ENSRNOG00000013351.
ENSRNOT00000049699; ENSRNOP00000049322; ENSRNOG00000013351.

GeneID

81022.

KEGG

rno:81022.

UCSC

RGD:708517. rat.

Organism-specific databases

CTD

134957.

RGD

708517. Stxbp5.

Phylogenomic databases

eggNOG

NOG289584.

GeneTree

ENSGT00390000000018.

HOGENOM

HOG000110929.

HOVERGEN

HBG083064.

InParanoid

Q9WU70.

KO

K08518.

OrthoDB

EOG4JHCDX.

Gene expression databases

ArrayExpress

Q9WU70.

Genevestigator

Q9WU70.

GermOnline

ENSRNOG00000013351. Rattus norvegicus.

Family and domain databases

Gene3D

2.130.10.10. 2 hits.

InterPro

IPR000664. Lethal2_giant.
IPR013905. Lgl_C.
IPR013577. LLGL2.
IPR001388. Synaptobrevin.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]

Pfam

PF08596. Lgl_C. 1 hit.
PF08366. LLGL. 1 hit.
PF00400. WD40. 2 hits.
[Graphical view]

PRINTS

PR00962. LETHAL2GIANT.

SMART

SM00320. WD40. 7 hits.
[Graphical view]

SUPFAM

SSF50978. WD40_like. 2 hits.

PROSITE

PS50892. V_SNARE. 1 hit.
PS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9WU70.

NextBio

614943.

Entry information

Entry name

STXB5_RAT

Accession

Primary (citable) accession number: Q9WU70
Secondary accession number(s): Q9WU71, Q9Z152

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	November 1, 1999
Last modified:	April 3, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program