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Q9WU70

- STXB5_RAT

UniProt

Q9WU70 - STXB5_RAT

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Protein

Syntaxin-binding protein 5

Gene

Stxbp5

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Inhibits translocation of GLUT4 from intracellular vesicles to the plasma membrane (By similarity). Plays a regulatory role in calcium-dependent exocytosis and neurotransmitter release. Inhibits membrane fusion between transport vesicles and the plasma membrane. May modulate the assembly of trans-SNARE complexes between transport vesicles and the plasma membrane. Competes with STXBP1 for STX1 binding.By similarity1 Publication

GO - Molecular functioni

  1. syntaxin-1 binding Source: RGD

GO - Biological processi

  1. exocytosis Source: UniProtKB-KW
  2. positive regulation of exocytosis Source: BHF-UCL
  3. protein transport Source: UniProtKB-KW
  4. regulation of blood coagulation Source: Ensembl
  5. regulation of gene expression Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Exocytosis, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin-binding protein 5
Alternative name(s):
Lethal(2) giant larvae protein homolog 3
Tomosyn-1
Gene namesi
Name:Stxbp5
Synonyms:Llgl3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi708517. Stxbp5.

Subcellular locationi

Cytoplasm 1 Publication. Cell membrane 1 Publication; Peripheral membrane protein 1 Publication. Cytoplasmic vesicle membrane 1 Publication; Peripheral membrane protein 1 Publication. Cell junctionsynapse 1 Publication. Cytoplasmic vesiclesecretory vesiclesynaptic vesicle 1 Publication
Note: Cytoplasmic, and associated with vesicular membranes and the plasma membrane. Detected at synapses and on synaptic vesicles.

GO - Cellular componenti

  1. acetylcholine-gated channel complex Source: Ensembl
  2. cell junction Source: UniProtKB-KW
  3. secretory granule Source: BHF-UCL
  4. synapse Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi724 – 7241S → A: Strongly reduces phosphorylation. 1 Publication
Mutagenesisi724 – 7241S → D: Reduces interaction with STX1A. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11521152Syntaxin-binding protein 5PRO_0000051246Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei724 – 7241Phosphoserine; by PKA1 Publication
Modified residuei760 – 7601PhosphoserineBy similarity
Modified residuei763 – 7631PhosphothreonineBy similarity
Modified residuei783 – 7831PhosphoserineBy similarity
Modified residuei786 – 7861PhosphoserineBy similarity

Post-translational modificationi

Phosphorylation by PKA reduces interaction with STX1A and enhances synaptic neurotransmitter release.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9WU70.
PRIDEiQ9WU70.

PTM databases

PhosphoSiteiQ9WU70.

Expressioni

Tissue specificityi

Isoform 1 is detected in heart, brain, lung, liver, skeletal muscle, kidney and testis. Isoform 2 is detected in brain and in testis. Isoform 3 is detected in testis.2 Publications

Gene expression databases

GenevestigatoriQ9WU70.

Interactioni

Subunit structurei

Part of a complex that contains STXBP5, STX4A and SNAP23 (By similarity). Interacts with STX1A and STX4A via its v-SNARE homology domain. Part of a complex that contains STX1, STXBP5, SNAP25 and SYT1.By similarity3 Publications

Protein-protein interaction databases

BioGridi249498. 2 interactions.
IntActiQ9WU70. 1 interaction.

Structurei

Secondary structure

1
1152
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi1089 – 114355Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1URQX-ray2.00A1086-1145[»]
ProteinModelPortaliQ9WU70.
SMRiQ9WU70. Positions 1088-1144.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WU70.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati62 – 9534WD 1Add
BLAST
Repeati102 – 14140WD 2Add
BLAST
Repeati146 – 18237WD 3Add
BLAST
Repeati201 – 23535WD 4Add
BLAST
Repeati241 – 27333WD 5Add
BLAST
Repeati295 – 33743WD 6Add
BLAST
Repeati345 – 37935WD 7Add
BLAST
Repeati401 – 47878WD 8Add
BLAST
Repeati506 – 620115WD 9Add
BLAST
Repeati634 – 69663WD 10Add
BLAST
Repeati795 – 85258WD 11Add
BLAST
Repeati861 – 93575WD 12Add
BLAST
Repeati940 – 98445WD 13Add
BLAST
Repeati998 – 102124WD 14Add
BLAST
Domaini1087 – 114761v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi22 – 287Poly-Gln
Compositional biasi585 – 5917Poly-Ser

Sequence similaritiesi

Belongs to the WD repeat L(2)GL family.Curated
Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation
Contains 14 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

eggNOGiNOG289584.
GeneTreeiENSGT00390000000018.
HOGENOMiHOG000110929.
HOVERGENiHBG083064.
InParanoidiQ9WU70.
KOiK08518.
OMAiRYTHDEA.
OrthoDBiEOG7DVD96.
PhylomeDBiQ9WU70.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR000664. Lethal2_giant.
IPR013905. Lgl_C_dom.
IPR013577. LLGL2.
IPR001388. Synaptobrevin.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08596. Lgl_C. 1 hit.
PF08366. LLGL. 1 hit.
PF00400. WD40. 2 hits.
[Graphical view]
PRINTSiPR00962. LETHAL2GIANT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 6 hits.
PROSITEiPS50892. V_SNARE. 1 hit.
PS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9WU70-1) [UniParc]FASTAAdd to Basket

Also known as: B-tomosyn

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRKFNIRKVL DGLTAGSSSA SQQQQQQQHP PGNREPEIQE TLQSEHFQLC
60 70 80 90 100
KTVRHGFPYQ PSALAFDPVQ KILAVGTQTG ALRLFGRPGV ECYCQHDSGA
110 120 130 140 150
AVIQLQFLIN EGALVSALAD DTLHLWNLRQ KRPAVLHSLK FCRERVTFCH
160 170 180 190 200
LPFQSKWLYV GTERGNIHIV NVESFTLSGY VIMWNKAIEL SSKSHPGPVV
210 220 230 240 250
HISDNPMDEG KLLIGFESGT VVLWDLKSKK ADYRYTYDEA IHSVAWHHEG
260 270 280 290 300
KQFICSHSDG TLTIWNVRSP TKPVQTITPH GKQLKDGKKP EPCKPILKVE
310 320 330 340 350
FKTTRSGEPF IILSGGLSYD TVGRRPCLTV MHGKSTAVLE MDYSIVDFLT
360 370 380 390 400
LCETPYPNDF QEPYAVVVLL EKDLVLIDLA QNGYPIFENP YPLSIHESPV
410 420 430 440 450
TCCEYFADCP VDLIPALYSV GARQKRQGYS KKEWPINGGN WGLGAQSYPE
460 470 480 490 500
IIITGHADGS IKFWDASAIT LQVLYKLKTS KVFEKSRNKD DRQNTDIVDE
510 520 530 540 550
DPYAIQIISW CPESRMLCIA GVSAHVIIYR FSKQEVVTEV IPMLEVRLLY
560 570 580 590 600
EINDVETPEG EQPPPLSTPV GSSTSQPIPP QSHPSTSSSS SDGLRDNVPC
610 620 630 640 650
LKVKNSPLKQ SPGYQTELVI QLVWVGGEPP QQITSLALNS SYGLVVFGNS
660 670 680 690 700
NGIAMVDYLQ KAVLLNLSTI ELYGSNDPYR REPRSPRKSR QPSGAGLCDI
710 720 730 740 750
TEGTVVPEDR CKSPTSGSSS PHNSDDEQKV NNFIEKVKTQ SRKFSKMVAS
760 770 780 790 800
DLAKMSRKLS LPTDLKPDLD VKDNSFSRSR SSSVTSIDKE SREAISALHF
810 820 830 840 850
CETFTRKADS SPSPCLWVGT TVGTAFVITL NLPLGPEQRL LQPVIVSPSG
860 870 880 890 900
TILRLKGAIL RMAFLDAAGC LMPPAYEPWT EHNVPEEKDE KEKLKKRRPV
910 920 930 940 950
SVSPSSSQEI SENQYAVICS EKQAKVISLP TQNCAYKQNI TETSFVLRGD
960 970 980 990 1000
IVALSNSVCL ACFCANGHIM TFSLPSLRPL LDVYYLPLTN MRIARTFCFA
1010 1020 1030 1040 1050
NSGQALYLVS PTEIQRLTYS QETCENLQEM LGELFTPVET PEAPNRGFFK
1060 1070 1080 1090 1100
GLFGGGAQSL DREELFGESS SGKASRSLAQ HIPGPGGIEG VKGAASGVVG
1110 1120 1130 1140 1150
ELARARLALD ERGQKLSDLE ERTAAMMSSA DSFSKHAHEM MLKYKDKKWY

QF
Length:1,152
Mass (Da):127,659
Last modified:November 1, 1999 - v1
Checksum:i1C07CABD8421CBD5
GO
Isoform 2 (identifier: Q9WU70-2) [UniParc]FASTAAdd to Basket

Also known as: M-tomosyn

The sequence of this isoform differs from the canonical sequence as follows:
     717-752: Missing.

Show »
Length:1,116
Mass (Da):123,638
Checksum:i034B99A939A987EA
GO
Isoform 3 (identifier: Q9WU70-3) [UniParc]FASTAAdd to Basket

Also known as: S-tomosyn

The sequence of this isoform differs from the canonical sequence as follows:
     717-769: Missing.

Show »
Length:1,099
Mass (Da):121,743
Checksum:iB199B25F2FE117CE
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei717 – 76953Missing in isoform 3. 1 PublicationVSP_016476Add
BLAST
Alternative sequencei717 – 75236Missing in isoform 2. 2 PublicationsVSP_016477Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92072 mRNA. Translation: AAD04756.1.
AF118889 mRNA. Translation: AAD27818.1.
AF118890 mRNA. Translation: AAD27819.1.
PIRiT42213.
RefSeqiNP_110470.1. NM_030843.2. [Q9WU70-2]
NP_848035.1. NM_178345.1. [Q9WU70-1]
NP_848036.1. NM_178346.1. [Q9WU70-3]
UniGeneiRn.96029.

Genome annotation databases

EnsembliENSRNOT00000040559; ENSRNOP00000044738; ENSRNOG00000013351. [Q9WU70-2]
ENSRNOT00000044778; ENSRNOP00000039502; ENSRNOG00000013351. [Q9WU70-3]
ENSRNOT00000049699; ENSRNOP00000049322; ENSRNOG00000013351. [Q9WU70-1]
GeneIDi81022.
KEGGirno:81022.
UCSCiRGD:708517. rat. [Q9WU70-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92072 mRNA. Translation: AAD04756.1 .
AF118889 mRNA. Translation: AAD27818.1 .
AF118890 mRNA. Translation: AAD27819.1 .
PIRi T42213.
RefSeqi NP_110470.1. NM_030843.2. [Q9WU70-2 ]
NP_848035.1. NM_178345.1. [Q9WU70-1 ]
NP_848036.1. NM_178346.1. [Q9WU70-3 ]
UniGenei Rn.96029.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1URQ X-ray 2.00 A 1086-1145 [» ]
ProteinModelPortali Q9WU70.
SMRi Q9WU70. Positions 1088-1144.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 249498. 2 interactions.
IntActi Q9WU70. 1 interaction.

PTM databases

PhosphoSitei Q9WU70.

Proteomic databases

PaxDbi Q9WU70.
PRIDEi Q9WU70.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000040559 ; ENSRNOP00000044738 ; ENSRNOG00000013351 . [Q9WU70-2 ]
ENSRNOT00000044778 ; ENSRNOP00000039502 ; ENSRNOG00000013351 . [Q9WU70-3 ]
ENSRNOT00000049699 ; ENSRNOP00000049322 ; ENSRNOG00000013351 . [Q9WU70-1 ]
GeneIDi 81022.
KEGGi rno:81022.
UCSCi RGD:708517. rat. [Q9WU70-1 ]

Organism-specific databases

CTDi 134957.
RGDi 708517. Stxbp5.

Phylogenomic databases

eggNOGi NOG289584.
GeneTreei ENSGT00390000000018.
HOGENOMi HOG000110929.
HOVERGENi HBG083064.
InParanoidi Q9WU70.
KOi K08518.
OMAi RYTHDEA.
OrthoDBi EOG7DVD96.
PhylomeDBi Q9WU70.

Miscellaneous databases

EvolutionaryTracei Q9WU70.
NextBioi 614943.
PROi Q9WU70.

Gene expression databases

Genevestigatori Q9WU70.

Family and domain databases

Gene3Di 2.130.10.10. 2 hits.
InterProi IPR000664. Lethal2_giant.
IPR013905. Lgl_C_dom.
IPR013577. LLGL2.
IPR001388. Synaptobrevin.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF08596. Lgl_C. 1 hit.
PF08366. LLGL. 1 hit.
PF00400. WD40. 2 hits.
[Graphical view ]
PRINTSi PR00962. LETHAL2GIANT.
SMARTi SM00320. WD40. 7 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 6 hits.
PROSITEi PS50892. V_SNARE. 1 hit.
PS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Tomosyn: a syntaxin-1-binding protein that forms a novel complex in the neurotransmitter release process."
    Fujita Y., Shirataki H., Sakisaka T., Asakura T., Ohya T., Kotani H., Yokoyama S., Nishioka H., Matsuura Y., Mizoguchi A., Scheller R.H., Takai Y.
    Neuron 20:905-915(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), PARTIAL PROTEIN SEQUENCE, FUNCTION, INTERACTION WITH STX1A AND STX1B, IDENTIFICATION IN A COMPLEX WITH STX1A; SNAP25 AND SYT1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Brain.
  2. "Three splicing variants of tomosyn and identification of their syntaxin-binding region."
    Yokoyama S., Shirataki H., Sakisaka T., Takai Y.
    Biochem. Biophys. Res. Commun. 256:218-222(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), INTERACTION WITH STX1A, TISSUE SPECIFICITY.
    Tissue: Kidney.
  3. "PKA-catalyzed phosphorylation of tomosyn and its implication in Ca2+-dependent exocytosis of neurotransmitter."
    Baba T., Sakisaka T., Mochida S., Takai Y.
    J. Cell Biol. 170:1113-1125(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF SER-724, PHOSPHORYLATION AT SER-724.
  4. "Structural basis for the inhibitory role of tomosyn in exocytosis."
    Pobbati A.V., Razeto A., Boeddener M., Becker S., Fasshauer D.
    J. Biol. Chem. 279:47192-47200(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1010-1145 IN COMPLEX WITH STX1A AND SNAP25.

Entry informationi

Entry nameiSTXB5_RAT
AccessioniPrimary (citable) accession number: Q9WU70
Secondary accession number(s): Q9WU71, Q9Z152
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: November 1, 1999
Last modified: November 26, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3