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Protein

Glycerol kinase 2

Gene

Gk2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Key enzyme in the regulation of glycerol uptake and metabolism.

Catalytic activityi

ATP + glycerol = ADP + sn-glycerol 3-phosphate.

Pathwayi: glycerol degradation via glycerol kinase pathway

This protein is involved in step 1 of the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol.
Proteins known to be involved in this subpathway in this organism are:
  1. Glycerol kinase 2 (Gk2), Glycerol kinase (Gk), Putative glycerol kinase 5 (Gk5)
This subpathway is part of the pathway glycerol degradation via glycerol kinase pathway, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol, the pathway glycerol degradation via glycerol kinase pathway and in Polyol metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei20 – 201SubstrateBy similarity
Binding sitei24 – 241ATPBy similarity
Binding sitei94 – 941SubstrateBy similarity
Binding sitei148 – 1481SubstrateBy similarity
Binding sitei259 – 2591SubstrateBy similarity
Binding sitei281 – 2811ATPBy similarity
Binding sitei326 – 3261ATP; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi427 – 4315ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycerol metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00618; UER00672.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol kinase 2 (EC:2.7.1.30)
Short name:
GK 2
Short name:
Glycerokinase 2
Alternative name(s):
ATP:glycerol 3-phosphotransferase 2
Glycerol kinase, testis specific 2
Gene namesi
Name:Gk2
Synonyms:Gk-rs2, Gkrs2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1329027. Gk2.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • mitochondrial outer membrane Source: UniProtKB-SubCell
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 554554Glycerol kinase 2PRO_0000343678Add
BLAST

Proteomic databases

EPDiQ9WU65.
MaxQBiQ9WU65.
PaxDbiQ9WU65.
PRIDEiQ9WU65.

PTM databases

iPTMnetiQ9WU65.
PhosphoSiteiQ9WU65.

Expressioni

Gene expression databases

BgeeiQ9WU65.
GenevisibleiQ9WU65. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000052226.

Structurei

3D structure databases

ProteinModelPortaliQ9WU65.
SMRiQ9WU65. Positions 13-514.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FGGY kinase family.Curated

Phylogenomic databases

eggNOGiKOG2517. Eukaryota.
COG0554. LUCA.
GeneTreeiENSGT00530000063143.
HOGENOMiHOG000222134.
HOVERGENiHBG002451.
InParanoidiQ9WU65.
KOiK00864.
OMAiKASMPET.
OrthoDBiEOG7VTDMZ.
PhylomeDBiQ9WU65.
TreeFamiTF321504.

Family and domain databases

InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WU65-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASKQTSAG PLVGAVVQGT NSTRFLVFNS KTAELVCSHQ VELTQEYPKE
60 70 80 90 100
GWVEQDPKEI LKSVYECIAK ACEKLAEVNI DISNIKAIGV SNQRETTVVW
110 120 130 140 150
DKFTGDPLYN AVVWLDLRTQ STVETLTKKI PGNSNFVKSK TGLPLSTYFS
160 170 180 190 200
AVKLRWMLDN LRPIQKAVEE GRAMFGTIDS WLIWCMTGGV NGGIHCTDVT
210 220 230 240 250
NACRTMLFNI HSLEWDKDLC DFFEIPMSIL PNVCSSSEIY GLMTSGALEG
260 270 280 290 300
VPISGCLGDQ SAALVGQMCF HEGQAKNTYG TGCFLLCNTG QKCVFSEHGL
310 320 330 340 350
LTTLAYKLGK NKPVFYALEG SVAIAGAVIR WLRDNFEIIT TSGEVENLAR
360 370 380 390 400
EVGTSYGCYF VPAFSGLYAP YWEPSARGII CGLTQFTNKC HIAFAALEAV
410 420 430 440 450
CFQTREILDA MNRDCGIPLS HLQVDGGMTN NRILMQLQAD ILHIPVVKSV
460 470 480 490 500
MPETTALGAA MAAGAAEGVN VWSLEPEDLS TILMERYEPQ IQATESEIRF
510 520 530 540 550
STWKRAVMKS MGWVTAKDPE NGDNPVFSCL PLGFFIVSSM TLLIGARCVS

TAEE
Length:554
Mass (Da):60,630
Last modified:November 1, 1999 - v1
Checksum:i8DFD5E85E74F5198
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF117734 mRNA. Translation: AAD24551.1.
BC050764 mRNA. Translation: AAH50764.1.
BC061147 mRNA. Translation: AAH61147.1.
CCDSiCCDS19454.1.
RefSeqiNP_034424.1. NM_010294.1.
UniGeneiMm.61206.

Genome annotation databases

EnsembliENSMUST00000059657; ENSMUSP00000052226; ENSMUSG00000050553.
GeneIDi14626.
KEGGimmu:14626.
UCSCiuc008yfz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF117734 mRNA. Translation: AAD24551.1.
BC050764 mRNA. Translation: AAH50764.1.
BC061147 mRNA. Translation: AAH61147.1.
CCDSiCCDS19454.1.
RefSeqiNP_034424.1. NM_010294.1.
UniGeneiMm.61206.

3D structure databases

ProteinModelPortaliQ9WU65.
SMRiQ9WU65. Positions 13-514.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000052226.

PTM databases

iPTMnetiQ9WU65.
PhosphoSiteiQ9WU65.

Proteomic databases

EPDiQ9WU65.
MaxQBiQ9WU65.
PaxDbiQ9WU65.
PRIDEiQ9WU65.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000059657; ENSMUSP00000052226; ENSMUSG00000050553.
GeneIDi14626.
KEGGimmu:14626.
UCSCiuc008yfz.1. mouse.

Organism-specific databases

CTDi2712.
MGIiMGI:1329027. Gk2.

Phylogenomic databases

eggNOGiKOG2517. Eukaryota.
COG0554. LUCA.
GeneTreeiENSGT00530000063143.
HOGENOMiHOG000222134.
HOVERGENiHBG002451.
InParanoidiQ9WU65.
KOiK00864.
OMAiKASMPET.
OrthoDBiEOG7VTDMZ.
PhylomeDBiQ9WU65.
TreeFamiTF321504.

Enzyme and pathway databases

UniPathwayiUPA00618; UER00672.

Miscellaneous databases

NextBioi286466.
PROiQ9WU65.
SOURCEiSearch...

Gene expression databases

BgeeiQ9WU65.
GenevisibleiQ9WU65. MM.

Family and domain databases

InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Retrotransposition of glycerol kinase-related genes from the X chromosome to autosomes: functional and evolutionary aspects."
    Pan Y., Decker W.K., Huq A.H., Craigen W.J.
    Genomics 59:282-290(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 129/Sv.
    Tissue: Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.

Entry informationi

Entry nameiGLPK2_MOUSE
AccessioniPrimary (citable) accession number: Q9WU65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: November 1, 1999
Last modified: May 11, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.