Reviewed, UniProtKB/Swiss-Prot Q9WU65 (GLPK2_MOUSE)

Names and origin

Protein names

Recommended name:
    Glycerol kinase 2
      Short name=Glycerokinase 2
      Short name=GK 2
    EC=2.7.1.30
Alternative name(s):
    Glycerol kinase, testis specific 2
    ATP:glycerol 3-phosphotransferase

Gene names

Name:	Gk2
Synonyms:	Gk-rs2, Gkrs2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	554 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism.
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate.
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.
Subcellular location	Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cytoplasm By similarity. Note: In sperm the majority of the enzyme is bound to mitochondria By similarity.
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Cellular component	Cytoplasm Membrane Mitochondrion Mitochondrion outer membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	internal side of plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 554	554	Glycerol kinase 2		PRO_0000343678
Regions
	Nucleotide binding	427 – 431	5	ATP By similarity
Sites
	Binding site	20	1	Substrate By similarity
	Binding site	24	1	ATP By similarity
	Binding site	94	1	Substrate By similarity
	Binding site	148	1	Substrate By similarity
	Binding site	259	1	Substrate By similarity
	Binding site	281	1	ATP By similarity
	Binding site	326	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9WU65-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 8DFD5E85E74F5198	FASTA	554	60,630
	10 20 30 40 50 60 MAASKQTSAG PLVGAVVQGT NSTRFLVFNS KTAELVCSHQ VELTQEYPKE GWVEQDPKEI 70 80 90 100 110 120 LKSVYECIAK ACEKLAEVNI DISNIKAIGV SNQRETTVVW DKFTGDPLYN AVVWLDLRTQ 130 140 150 160 170 180 STVETLTKKI PGNSNFVKSK TGLPLSTYFS AVKLRWMLDN LRPIQKAVEE GRAMFGTIDS 190 200 210 220 230 240 WLIWCMTGGV NGGIHCTDVT NACRTMLFNI HSLEWDKDLC DFFEIPMSIL PNVCSSSEIY 250 260 270 280 290 300 GLMTSGALEG VPISGCLGDQ SAALVGQMCF HEGQAKNTYG TGCFLLCNTG QKCVFSEHGL 310 320 330 340 350 360 LTTLAYKLGK NKPVFYALEG SVAIAGAVIR WLRDNFEIIT TSGEVENLAR EVGTSYGCYF 370 380 390 400 410 420 VPAFSGLYAP YWEPSARGII CGLTQFTNKC HIAFAALEAV CFQTREILDA MNRDCGIPLS 430 440 450 460 470 480 HLQVDGGMTN NRILMQLQAD ILHIPVVKSV MPETTALGAA MAAGAAEGVN VWSLEPEDLS 490 500 510 520 530 540 TILMERYEPQ IQATESEIRF STWKRAVMKS MGWVTAKDPE NGDNPVFSCL PLGFFIVSSM 550 TLLIGARCVS TAEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Retrotransposition of glycerol kinase-related genes from the X chromosome to autosomes: functional and evolutionary aspects." Pan Y., Decker W.K., Huq A.H., Craigen W.J. Genomics 59:282-290(1999) [PubMed: 10444329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 129/Sv. Tissue: Testis.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF117734 mRNA. Translation: AAD24551.1. BC050764 mRNA. Translation: AAH50764.1. BC061147 mRNA. Translation: AAH61147.1.
IPI	IPI00123975.
RefSeq	NP_034424.1.
UniGene	Mm.61206
3D structure databases
HSSP	HSSP built from PDB template 1GLJ based on UniProtKB P08859.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9WU65.
PTM databases
PhosphoSite	Q9WU65.
Proteomic databases
PRIDE	Q9WU65.
Genome annotation databases
Ensembl	ENSMUST00000059657; ENSMUSP00000052226; ENSMUSG00000050553; Mus musculus. [Genome view]
GeneID	14626.
KEGG	mmu:14626.
Organism-specific databases
CTD	14626.
MGI	MGI:1329027. Gk2.
Phylogenomic databases
HOGENOM	Q9WU65.
HOVERGEN	Q9WU65.
OMA	LMERYEP.
Gene expression databases
ArrayExpress	Q9WU65.
Bgee	Q9WU65.
Genevestigator	Q9WU65.
Family and domain databases
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	286466.
SOURCE	Search...

Entry information

Entry name

GLPK2_MOUSE

Accession

Primary (citable) accession number: Q9WU65

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	November 1, 1999
Last modified:	November 3, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)